Abstract:
:Repeated passages of vaccinia virus in increasing concentrations of hydroxyurea followed by plaque purification resulted in the isolation of variants capable of growth in 5 mM hydroxyurea, a drug concentration which inhibited the reproduction of wild-type vaccinia virus 1,000-fold. Analyses of viral protein synthesis by using [35S]methionine pulse-labeling at intervals throughout the infection cycle revealed that all isolates overproduced a 34,000-molecular-weight (MW) early polypeptide. Measurement of ribonucleoside-diphosphate reductase (EC 1.17.4.1) activity after infection indicated that 4- to 10-fold more activity was induced by hydroxyurea-resistant viruses than by the wild-type virus. A two-step partial purification which yielded greater than 90% of the induced ribonucleotide reductase activity in the fraction obtained by 35% saturation with ammonium sulfate resulted in a substantial enrichment for the 34,000-MW protein from extracts of wild-type and hydroxyurea-resistant-virus-infected, but not mock-infected, cells. In the presence of the drug, the isolates incorporated [3H]thymidine into DNA earlier and at a rate substantially greater than that of the wild type, although the onset of DNA synthesis was delayed in both cases. In the absence of the drug, the attainment of a maximum viral DNA synthesis rate was accelerated after infection by drug-resistant isolates. The drug resistance trait was markedly unstable in all isolates. In the absence of selective pressure, plaque-purified isolates readily segregated progeny that displayed a wide range of resistance phenotypes. The results of this study indicate that vaccinia virus encodes a subunit of ribonucleotide reductase which is a 34,000-MW early protein whose overproduction confers hydroxyurea resistance on reproducing viruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Slabaugh MB,Mathews CKdoi
10.1128/JVI.60.2.506-514.1986subject
Has Abstractpub_date
1986-11-01 00:00:00pages
506-14issue
2eissn
0022-538Xissn
1098-5514journal_volume
60pub_type
杂志文章abstract::Mice and cattle injected with plasmids encoding bovine herpesvirus 1 (BHV-1) glycoproteins developed gene-specific antibody responses capable of neutralizing BHV-1. The ability of animals to respond serologically to DNA injections was in part dependent on the quantity of DNA injected and was also negatively affected b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5664-5667.1993
更新日期:1993-09-01 00:00:00
abstract::Several lines of evidence suggest that various cofactors may be required for prion replication. PrP binds to polyanions, and RNAs were shown to promote the conversion of PrP(C) into PrP(Sc) in vitro. In the present study, we investigated strain-specific differences in RNA requirement during in vitro conversion and the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01286-12
更新日期:2012-10-01 00:00:00
abstract::Neurotropic coronavirus induces acute encephalomyelitis and demyelination in mice. Infection of BALB/c (H-2(d)) mice expressing a dominant negative gamma interferon (IFN-gamma) receptor specifically in oligodendrocytes was examined to determine the influence of IFN-gamma signaling on pathogenesis. Inhibition of IFN-ga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02373-09
更新日期:2010-03-01 00:00:00
abstract::Infectious leukemia virus production by two chronically infected NIH/MOL lines was strongly inhibited by interferon treatment of the cells. The corresponding degree of inhibition in JLSV-11 cells was much lower. Multiplication of encephalomyocarditis virus in all three cell lines was barely affected by interferon trea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.3.1017-1027.1983
更新日期:1983-03-01 00:00:00
abstract::A series of recombinant molecules were constructed which direct the expression of the easily assayed gene chloramphenicol acetyltransferase. We have used these recombinants to show that the 73/72-base-pair tandem repeat unit from the Moloney murine sarcoma virus long terminal repeat shares a number of properties with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.183-189.1984
更新日期:1984-01-01 00:00:00
abstract::Infectious bursal disease virus (IBDV) is a double-stranded RNA (dsRNA) virus of the Birnaviridae family. Its two genome segments are encapsidated together with multiple copies of the viral RNA-dependent RNA polymerase, VP1, in a single-shell capsid that is composed of VP2 and VP3. In this study we identified the doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11301-11311.2002
更新日期:2002-11-01 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68) is a gammaherpesvirus that was first isolated from murid rodents. MHV68 establishes a latent infection in the spleen and other lymphoid organs. Several gammaherpesviruses, including herpesvirus saimiri, human herpesvirus 8, and MHV68, encode proteins with extensive homology to the D-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7016-7023.2000
更新日期:2000-08-01 00:00:00
abstract::Interferon (IFN) is an important immune system molecule capable of inducing an antiviral state within cells. Herpes simplex virus type 1 (HSV-1) replication is somewhat reduced in tissue culture in the presence of IFN, presumably due to decreased viral transcription. Here, we show mutations that inactivate immediate-e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.2052-2056.2000
更新日期:2000-02-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) primary infection is characterized by the use of CCR5 as a coreceptor for viral entry, which is associated with the non-syncytium-inducing (NSI) phenotype in lymphoid cells. Syncytium-inducing (SI) variants of HIV-1 appear in advanced stages of HIV-1 infection and are charac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10319-10325.2001
更新日期:2001-11-01 00:00:00
abstract::The amount and distribution of viral DNA were established in a horse acutely infected with the Wyoming strain of equine infectious anemia virus (EIAV). The highest concentration of viral DNA were found in the liver, lymph nodes, bone marrow, and spleen. The kidney, choroid plexus, and peripheral blood leukocytes also ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5194-5200.1989
更新日期:1989-12-01 00:00:00
abstract::Recombinant retroviruses containing the trans activator genes of human T-cell leukemia virus (HTLV) type II and human T-cell lymphotropic virus type III were constructed. The trans activator genes tat II and tat III were inserted into the murine retroviral vector pZIPNEOSV(X)1. Recombinant plasmids were transfected in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.379-384.1986
更新日期:1986-01-01 00:00:00
abstract::The Rel/NF-kappa B family of proteins includes several interacting cellular transcription factors and the v-Rel oncoprotein of the avian Rev-T retrovirus. We report the isolation of a chicken cDNA for the NF-kappa B p52 precursor protein p100. Full-length p100 only weakly binds DNA in vitro; removal of the ankyrin-lik...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7612-7617.1993
更新日期:1993-12-01 00:00:00
abstract::Previous experiments have demonstrated that adenovirus infection of human and mouse cells leads to an E1A-dependent activation of the DNA-binding capacity of a cellular transcription factor termed E2F. E2F binds to two sites in the adenovirus E2 early promoter which have been shown to be critical for E1A-dependent E2 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3643-3650.1989
更新日期:1989-09-01 00:00:00
abstract::To examine the prerequisites for cleavage activation of the hemagglutinin of human influenza viruses, a cDNA clone obtained from strain A/Port Chalmers/1/73 (serotype H3) was subjected to site-directed mutagenesis and expressed in CV-1 cells by using a simian virus 40 vector. The number of basic residues at the cleava...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3530-3537.1991
更新日期:1991-07-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is treated with interferon (IFN)-based therapy. The mechanisms by which IFN suppresses HCV replication are not known, and only limited efficacy is achieved with therapy because the virus directs mechanisms to resist the host IFN response. In the present study we characterized the effe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.3898-3912.2003
更新日期:2003-04-01 00:00:00
abstract::A limited period of chemotherapy during primary immunodeficiency virus infection might provide a long-term clinical benefit even if treatment is initiated at a time point when virus is already detectable in plasma. To evaluate this strategy, we infected rhesus macaques with the pathogenic simian/human immunodeficiency...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1704-1711.2000
更新日期:2000-02-01 00:00:00
abstract::ICP4 is a herpesvirus-encoded protein that is expressed during the immediate-early phase of productive infection and is required for efficient transcription of viral genes during the early and late phases of infection. Previous studies have shown that ICP4 is a component of specific protein-DNA complexes but have not ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.696-704.1989
更新日期:1989-02-01 00:00:00
abstract::A restriction map was constructed for the 17.5-kilobase SalI C fragment of SP82 DNA. Unfractionated SP82 DNA, the SalI C fragment, and restriction fragments derived from SalI-C were used as templates for in vitro synthesis by the Bacillus subtilis RNA polymerase, and the resulting transcripts were analyzed by gel elec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.372-382.1981
更新日期:1981-01-01 00:00:00
abstract::The family Caliciviridae is divided into four genera and consists of single-stranded RNA viruses with hosts ranging from humans to a wide variety of animals. Human caliciviruses are the major cause of outbreaks of acute nonbacterial gastroenteritis, whereas animal caliciviruses cause various host-dependent illnesses w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6469-6479.2004
更新日期:2004-06-01 00:00:00
abstract::Immunosuppressed patients are at risk for developing Epstein-Barr Virus (EBV)-positive lymphomas that express the major EBV oncoprotein, LMP1. Although increasing evidence suggests that a small number of lytically infected cells may promote EBV-positive lymphomas, the impact of enhanced lytic gene expression on the ab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00770-12
更新日期:2012-08-01 00:00:00
abstract::The mammalian APOBEC3 proteins are an important component of the cellular innate immune response to retroviral infection. APOBEC3G can extinguish HIV-1 infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity that attacks the nascent viral cDNA during reverse transcription, caus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00056-10
更新日期:2010-07-01 00:00:00
abstract::Viruses with spindle-shaped virions are abundant in diverse environments. Over the years, such viruses have been isolated from a wide range of archaeal hosts. Evolutionary relationships between them remained enigmatic, however. Here, using structural proteins as markers, we define familial ties among these "dark horse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02941-13
更新日期:2014-02-01 00:00:00
abstract::We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved;...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-15
更新日期:2015-07-01 00:00:00
abstract::The generation of cytopathic effects by murine leukemia viruses (MLVs) in different cell types correlates with the ability of the virus to induce thymic lymphoma. We showed that the induction of apoptosis in mink epithelial cells by mink cell focus-forming (MCF) MLV infection results in the accumulation of high levels...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02291-07
更新日期:2008-03-01 00:00:00
abstract::We have previously reported that infection with herpes simplex virus type 1 (HSV-1) activates expression of the human immunodeficiency virus type 1 (HIV-1) provirus in T cells. Activation of the HIV-1 provirus correlated with the activation of binding of 55- and 85-kDa proteins to the kappa B enhancer and binding of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4427-4431.1993
更新日期:1993-07-01 00:00:00
abstract:UNLABELLED:Bovine papillomavirus 1 E6 interacts with two similar proteins that regulate cell attachment and cell migration called paxillin (PXN) and HIC-5 (also known as HIC5, ARA55, HIC-5, TSC-5, and TGFB1I1). Despite the similarity between HIC-5 and paxillin, paxillin is required for E6 to transform mouse embryo fibr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00552-14
更新日期:2014-09-01 00:00:00
abstract::Resistance or susceptibility of various mouse strains to central nervous system disease caused by different strains of coronavirus is well known. Data from the present study draw attention to an additional, genetically determined mechanism controlling CV infections. The resistance to A59 and JHM virus (JHMV) associate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3371-3377.1988
更新日期:1988-09-01 00:00:00
abstract::Numerous deaths of koi and common carp (Cyprinus carpio) were observed on many farms throughout Israel, resulting in severe financial losses. The lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. Diseased fish exhib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.1983-1991.2005
更新日期:2005-02-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection is frequently associated with extrahepatic manifestations, including nonmalignant and malignant B-cell lymphoproliferative disorders. It has been reported that specific changes or recurring motifs in the amino acid sequence of the HCV hypervariable region 1 (HVR1) may be assoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02104-06
更新日期:2007-05-01 00:00:00
abstract::The size of the product RNA synthesized by the poliovirus RNA polymerase and host factor was significantly affected by the type of column chromatography used to purify the polymerase. Dimer length product RNA was synthesized by the polymerase purified by chromatography on hydroxylapatite. This contrasted with the mono...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.611-614.1987
更新日期:1987-02-01 00:00:00