Abstract:
:Treatment of human immunodeficiency virus type 1 (HIV-1)-infected CEM cell cultures with escalating concentrations of the quinoxaline S-2720 resulted in an ordered appearance of single and multiple mutant virus strains that gradually became resistant to the quinoxaline and other nonnucleoside reverse transcriptase (RT) inhibitors (NNRTIs). A novel mutation, Pro225His, consistently appeared in a Val106Ala RT-mutated genetic background. The contribution of this mutation to the resistance of the mutant HIV-1 RT to NNRTIs was additive to the resistance caused by the Val106Ala mutation. Interestingly, site-directed mutagenesis studies revealed that the Pro225His-mutated RT had acquired markedly greater sensitivity to bis(heteroaryl)piperazine (BHAP U-90152) (delavirdine) but not to any of the other NNRTIs. The kinetics of inhibition of the Pro225His mutant RT by the NNRTIs (including BHAP U-90152) was not substantially different from that observed for the wild-type RT. The hypersensitivity of the mutant enzyme and virus to BHAP U-90152 could be rationally explained by the molecular-structural determinants of the RT-BHAP complex, which has recently been resolved by X-ray crystallography.
journal_name
J Viroljournal_title
Journal of virologyauthors
Pelemans H,Esnouf R,Dunkler A,Parniak MA,Vandamme AM,Karlsson A,De Clercq E,Kleim JP,Balzarini Jdoi
10.1128/JVI.71.11.8195-8203.1997subject
Has Abstractpub_date
1997-11-01 00:00:00pages
8195-203issue
11eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::The gene encoding the precursor polypeptide of the Marek's disease herpesvirus (MDHV) secretory glycoprotein gp57-65 (formerly identified as A antigen) has been sequenced. Previous results had localized the gene to a 4.6-kilobase (kb) segment of the BamHI B fragment in the unique long region of the MDHV genome. S1 nuc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2373-2379.1988
更新日期:1988-07-01 00:00:00
abstract::Virus-specific cytotoxic T lymphocytes (CTL) exert intense selection pressure on replicating simian immunodeficiency virus (SIV) and human immunodeficiency virus type 1 (HIV-1) in infected individuals. The immunodominant Mamu-A(*)01-restricted Gag p11C, C-M epitope is highly conserved among all sequenced isolates of S...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12572-12578.2003
更新日期:2003-12-01 00:00:00
abstract::Inclusion bodies are a characteristic feature of ebolavirus infections in cells. They contain large numbers of preformed nucleocapsids, but their biological significance has been debated, and they have been suggested to be aggregates of viral proteins without any further biological function. However, recent data for o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01525-12
更新日期:2012-11-01 00:00:00
abstract::Members of the tripartite interaction motif (TRIM) family of E3 ligases are emerging as critical regulators of innate immunity. To identify new regulators, we carried out a screen of 43 human TRIM proteins for the ability to activate NF-κB, AP-1, and interferon, hallmarks of many innate immune signaling pathways. We i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01804-12
更新日期:2013-01-01 00:00:00
abstract::p60src, the transforming protein of Rous sarcoma virus, was found to contain 0.5 to 0.9 mol of total phosphate per mol of polypeptide. The protein is known to be phosphorylated at two sites, a serine in the amino-terminal domain and a tyrosine in the carboxy-terminal domain. Because our indirect analysis suggests that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.3.813-820.1982
更新日期:1982-03-01 00:00:00
abstract::Empty capsids of foot-and-mouth disease virus (FMDV) type A22 Iraq 24/64, whose structure has been solved by X-ray crystallography, are unusual for picornaviruses since they contain VP2 and VP4, the cleavage products of the protein precursor VP0. Both the N terminus of VP1 and the C terminus of VP4, which pack togethe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9743-9752.1997
更新日期:1997-12-01 00:00:00
abstract::A prerequisite for understanding the molecular function of the human cytomegalovirus (HCMV) gH (UL75)-gL (UL115) complex is a detailed knowledge of the structure of this complex in its functional form, as it is present in mature virions. The gH protein is known to be a component of a 240-kDa envelope complex designate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5391-5398.1997
更新日期:1997-07-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV/human herpesvirus 8 [HHV-8]) is a gamma-2-herpesvirus responsible for Kaposi's sarcoma as well as primary effusion lymphoma (PEL). KSHV is a lymphotropic virus that has pirated many mammalian genes involved in inflammation, cell cycle control, and angiogenesis. Among these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.57-67.2003
更新日期:2003-01-01 00:00:00
abstract::Incorporation of the vaccinia virus A36R protein into the outer membrane of intracellular enveloped virions (IEV) is dependent on expression of the A33R protein. Possible interactions of the 200-amino-acid cytoplasmic domain of the A36R protein with itself or with the cytoplasmic domain of the A33R, A34R, B5R, or F12L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4113-4126.2003
更新日期:2003-04-01 00:00:00
abstract::The subgroup A to E avian sarcoma and leukosis viruses (ASLVs) are highly related and are thought to have evolved from a common ancestor. These viruses use distinct cell surface proteins as receptors to gain entry into avian cells. Chickens have evolved resistance to infection by the ASLVs. We have identified the muta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.24.13489-13500.2004
更新日期:2004-12-01 00:00:00
abstract::Polymorphic basic residues near the C terminus of the prion protein (PrP) in humans and sheep appear to protect against prion disease. In heterozygotes, inhibition of prion formation appears to be dominant negative and has been simulated in cultured cells persistently infected with scrapie prions. The results of nucle...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4351-4360.2000
更新日期:2000-05-01 00:00:00
abstract::Transient transfection experiments indicated (i) that E6 protein of non-cancer-associated cutaneous human papillomavirus type 1 (HPV-1) can inhibit p53-mediated transcriptional transactivation in both p53-deficient human cells (H358) and normal rat cells (3Y1), but those of cancer-associated cutaneous HPV-5, -8, and -...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4656-4661.1994
更新日期:1994-07-01 00:00:00
abstract::Human papillomavirus (HPV) type 16 E6 (16E6) is an oncogenic, multifunctional nuclear protein that induces p53 degradation and perturbs normal cell cycle control, leading to immortalization and transformation of infected keratinocytes and epithelial cells. Although it is unclear how 16E6 disrupts the epigenetic profil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13232-13247.2003
更新日期:2003-12-01 00:00:00
abstract::Previous studies with the herpes simplex virus type 1 X type 2 intertypic recombinant RS6 suggested that the genomic region from 0.11 to 0.14 map units is involved in neurovirulence (R. T. Javier, R. L. Thompson, and J. G. Stevens, J. Virol. 61:1978-1984, 1987). To study this further, we isolated an RS6-derived herpes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.4.1381-1387.1988
更新日期:1988-04-01 00:00:00
abstract::Chronic viruses such as herpes simplex virus 1 (HSV-1) evade the hosts' immune system by inducing the exhaustion of antiviral T cells. In the present study, we found that exhausted HSV-specific CD8+ T cells, with elevated expression of programmed death ligand-1 (PD-1) and lymphocyte activation gene-3 (LAG-3) receptors...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00827-19
更新日期:2019-08-28 00:00:00
abstract:UNLABELLED:The increasing number of zoonotic infections caused by influenza A virus (IAV) subtypes of avian origin (e.g., H5N1 and H7N9) in recent years underscores the need to better understand the factors driving IAV evolution and diversity. To evaluate the current feasibility of global analyses to contribute to this...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01573-15
更新日期:2015-11-01 00:00:00
abstract::The involvement of mouse mammary tumor virus (MTV) in the development of mammary tumors of nonviral etiology in BALB/c mice was studied by measuring the levels of MTV RNA, MTV DNA, and MTV proteins in spontaneously arising and hormonally, chemically, and/or physically induced mammary tumors of BALB/c females. The foll...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.1.63-72.1979
更新日期:1979-07-01 00:00:00
abstract::The isolation of a temperature phage specific for the photosynthetic bacterium Rhodopseudomonas spheroides is reported. This phage, Rphi-1, establishes a state of lysogeny and can be induced from the prophage state by exposure to mitomycin C or UV irradiation. Mutants of Rphi-1 which grow on a standard laboratory stra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1288-1292.1974
更新日期:1974-11-01 00:00:00
abstract::We previously showed that the cellular proteins ZEB1 and ZEB2/SIP1 both play key roles in regulating the latent-lytic switch of Epstein-Barr Virus (EBV) by repressing BZLF1 gene expression. We investigated here the effects of cellular microRNA (miRNA) 200 (miR200) family members on the EBV infection status of cells. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00923-10
更新日期:2010-10-01 00:00:00
abstract::The Epstein-Barr virus (EBV) lytic activator genes bzlf1 and brlf1 are conventionally referred to as immediate-early (IE) genes. However, previous studies showed that the earliest expression of these genes was blocked by cycloheximide when the EBV lytic cycle was induced by histone deacetylase (HDAC) inhibitors and pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01415-10
更新日期:2010-12-01 00:00:00
abstract::We have isolated a new type of temperature-sensitive mutant of simian virus 40 (SV40) that is capable of productive infection in permissive cells but not of maintenance of viral DNA integration in transformed cells at the conditional temperature. Virus development is induced when cells transformed by this mutant are s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.374-381.1976
更新日期:1976-08-01 00:00:00
abstract::Retroviruses have been isolated from the tissues of human leukemia patients. Previous studies have shown that these isolates share some antigenic determinants with the family of viruses isolated from the woolly monkey and gibbon ape and that they exhibit partial nuclei acid homology with this same group of viruses. We...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.2.390-401.1980
更新日期:1980-05-01 00:00:00
abstract::Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus related to rhabdoviruses and paramyxoviruses. Unlike animal viruses of these two families, BDV transcribes RNAs in the nuclei of infected cells and produces high levels of transcripts containing multiple open reading frames. Previous Northern blot ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5007-5012.1994
更新日期:1994-08-01 00:00:00
abstract::In wild aquatic birds and poultry around the world, influenza A viruses carrying 15 antigenic subtypes of hemagglutinin (HA) and 9 antigenic subtypes of neuraminidase (NA) have been described. Here we describe a previously unidentified antigenic subtype of HA (H16), detected in viruses circulating in black-headed gull...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2814-2822.2005
更新日期:2005-03-01 00:00:00
abstract::Interferon (IFN) signal transduction involves interferon regulatory factors (IRF). Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four IRF homologues: viral IRF 1 (vIRF-1) to vIRF-4. Previous functional studies revealed that the first exon of vIRF-2 inhibited alpha/beta interferon (IFN-alpha/beta) signaling. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.6.3092-3097.2006
更新日期:2006-03-01 00:00:00
abstract::A panel of seven monoclonal antibodies against the relatively conserved CD4-binding domain on human immunodeficiency virus type 1 (HIV-1) gp120 was generated by immunizing mice with purified gp120. These monoclonal antibodies reacted specifically with gp120 in an enzyme-linked immunosorbent assay and Western blots (im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3579-3585.1989
更新日期:1989-09-01 00:00:00
abstract::Polyomavirus large T antigen has an N-terminal domain of approximately 260 amino acids which can immortalize primary cells but lacks sequences known to be required for DNA binding and replication. Treatment of full-length large T with either V8 protease or chymotrypsin yields an N-terminal fragment of 36 to 40 kDa and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.668-673.1994
更新日期:1994-02-01 00:00:00
abstract::The open reading frame III of Borna disease virus (BDV) codes for a protein with a mass of 16 kDa, named p16 or BDV-M. p16 was described as an N-glycosylated protein in several previous publications and therefore was termed gp18, although the amino acid sequence of p16 does not contain any regular consensus sequence f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.24.12098-12104.2001
更新日期:2001-12-01 00:00:00
abstract::Apoptosis of infected cells is critically involved in antiviral defense. Apoptosis, however, may also support the release and spread of viruses. Although the elimination of infected hepatocytes is required to combat hepatitis B virus (HBV) infection, it is still unknown which consequences hepatocyte apoptosis has for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00653-10
更新日期:2010-11-01 00:00:00
abstract::Human norovirus (HuNoV) is a leading cause of acute gastroenteritis in both developed and developing countries. Studies of HuNoV host cell interactions are limited by the lack of a simple, robust cell culture system. Due to their diverse HuNoV-like biological features, including histo-blood group antigen (HBGA) bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00869-19
更新日期:2019-10-29 00:00:00