当前位置： SCI文献检索 > JOURNAL OF VIROLOGY期刊下所有文献 > Production of infectious human cytomegalovirus virions is inhibited by drugs that disrupt calcium homeostasis in the endoplasmic reticulum.

Production of infectious human cytomegalovirus virions is inhibited by drugs that disrupt calcium homeostasis in the endoplasmic reticulum.

Abstract：

:We previously reported that human cytomegalovirus (HCMV) infection induces endoplasmic reticulum (ER) stress, resulting in activation of the unfolded protein response (UPR). Although some normal consequences of UPR activation (e.g., translation attenuation) are detrimental to viral infection, we have previously shown that HCMV infection adapts the UPR to benefit the viral infection (14). For example, UPR-induced translation attenuation is inhibited by viral infection, while potentially beneficial aspects of the UPR are maintained. In the present work, we tested the ability of HCMV to overcome a robust induction of the UPR by the drugs thapsigargin and clotrimazole (CLT), which disrupt ER calcium homeostasis. A 24-h treatment with these drugs beginning at 48, 72, or 96 h postinfection (hpi) completely inhibited further production of infectious virions. HCMV could not overcome the inhibition of global translation by CLT; however, between 48 and 72 hpi, HCMV overcame translational inhibition caused by thapsigargin. Despite the restoration of translation in thapsigargin, the accumulation of immediate-early and early gene products was modestly retarded (50% or less), whereas the accumulation of an early-late and late gene product was significantly retarded. Electron microscopic analysis shows that the drugs severely disrupt the maturation of HCMV virions. This can be accounted for by both the retarded accumulation of late gene products and the drug-induced depletion of ER calcium, which disrupts critical cellular functions needed for maturation.

journal_name

J Virol

journal_title

Journal of virology

authors

Isler JA,Maguire TG,Alwine JC

doi

10.1128/JVI.79.24.15388-15397.2005

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

15388-97

issue

24

eissn

0022-538X

issn

1098-5514

pii

79/24/15388

journal_volume

79

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGY文献大全
  • A novel protease processing site in the transframe protein of human T-cell leukemia virus type 1 PR76(gag-pro) defines the N terminus of RT.

    abstract：:The genomic RNA of human T-cell leukemia virus type 1 encodes three polyproteins, Gag, Gag-Pro, and Gag-Pro-Pol, which are translated as a result of no, one, and two frameshifts, respectively. In this report we demonstrate that the 77 residues encoded at the C terminus of the Gag-Pro precursor can be collectively dete...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.76.24.13101-13105.2002

    authors： Heidecker G,Hill S,Lloyd PA,Derse D

    更新日期：2002-12-01 00:00:00

  • Fine structure mapping of five temperature-sensitive mutants in the 22- and 147-kilodalton subunits of vaccinia virus DNA-dependent RNA polymerase.

    abstract：:We have mapped the temperature-sensitive (ts) lesions of three mutants, ts51, ts53, and ts65, and two other mutants, ts7 and ts20, to regions on the vaccinia virus genome that encode the 147- and 22-kilodalton subunits of the viral DNA-dependent RNA polymerase, respectively. Plasmid and bacteriophage clones from the H...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.63.2.705-713.1989

    authors： Thompson CL,Hooda-Dhingra U,Condit RC

    更新日期：1989-02-01 00:00:00

  • The barley stripe mosaic virus 58-kilodalton beta(b) protein is a multifunctional RNA binding protein.

    abstract：:The barley stripe mosaic virus (BSMV) beta(b) gene product is the major viral nonstructural protein synthesized during early stages of the infection cycle and is required for systemic movement of the virus. To examine the biochemical properties of beta(b), a histidine tag was engineered at the amino terminus and the p...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.71.2.1538-1546.1997

    authors： Donald RG,Lawrence DM,Jackson AO

    更新日期：1997-02-01 00:00:00

  • Membrane-associated DNase activity controlled by genes 46 and 47 of bacteriophage T4D and elevated DNase activity associated with the T4 das mutation.

    abstract：:Lethal, amber mutations in T4 genes 46 and 47 cause incomplete degradation of host DNA, premature arrest of phage DNA synthesis, accumulation of abnormal DNA replication intermediates, and defective recombination. These phenotypes can be explained by the hypothesis that genes 46 and 47 control a DNA exonuclease, but i...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.40.1.65-77.1981

    authors： Mickelson C,Wiberg JS

    更新日期：1981-10-01 00:00:00

  • Switch to unusual amino acids at codon 215 of the human immunodeficiency virus type 1 reverse transcriptase gene in seroconvertors infected with zidovudine-resistant variants.

    abstract：:Sequences of the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) domain were determined by direct sequencing of HIV-1 RNA in successive plasma samples from eight seroconverting patients infected with virus bearing the T215Y/F amino acid substitution associated with zidovudine (ZDV) resistance. A...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.72.5.3520-3523.1998

    authors： Yerly S,Rakik A,De Loes SK,Hirschel B,Descamps D,Brun-Vézinet F,Perrin L

    更新日期：1998-05-01 00:00:00

  • Conserved determinants for membrane association of nonstructural protein 5A from hepatitis C virus and related viruses.

    abstract：:Nonstructural protein 5A (NS5A) is a membrane-associated essential component of the hepatitis C virus (HCV) replication complex. An N-terminal amphipathic alpha helix mediates in-plane membrane association of HCV NS5A and at the same time is likely involved in specific protein-protein interactions required for the ass...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01279-06

    authors： Brass V,Pal Z,Sapay N,Deléage G,Blum HE,Penin F,Moradpour D

    更新日期：2007-03-01 00:00:00

  • TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.

    abstract：:Members of the tripartite interaction motif (TRIM) family of E3 ligases are emerging as critical regulators of innate immunity. To identify new regulators, we carried out a screen of 43 human TRIM proteins for the ability to activate NF-κB, AP-1, and interferon, hallmarks of many innate immune signaling pathways. We i...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01804-12

    authors： Uchil PD,Hinz A,Siegel S,Coenen-Stass A,Pertel T,Luban J,Mothes W

    更新日期：2013-01-01 00:00:00

  • Imparting temperature sensitivity and attenuation in ferrets to A/Puerto Rico/8/34 influenza virus by transferring the genetic signature for temperature sensitivity from cold-adapted A/Ann Arbor/6/60.

    abstract：:The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.78.2.995-998.2004

    authors： Jin H,Zhou H,Lu B,Kemble G

    更新日期：2004-01-01 00:00:00

  • The major core protein P4a (A10L gene) of vaccinia virus is essential for correct assembly of viral DNA into the nucleoprotein complex to form immature viral particles.

    abstract：:The vaccinia virus (VV) A10L gene codes for a major core protein, P4a. This polypeptide is synthesized at late times during viral infection and is proteolytically cleaved during virion assembly. To investigate the role of P4a in the virus life cycle and morphogenesis, we have generated an inducer-dependent conditional...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.75.13.5778-5795.2001

    authors： Heljasvaara R,Rodríguez D,Risco C,Carrascosa JL,Esteban M,Rodríguez JR

    更新日期：2001-07-01 00:00:00

  • Characterization of murine gammaherpesvirus 68 glycoprotein B.

    abstract：:Murine gammaherpesvirus 68 (MHV-68) glycoprotein B (gB) was identified in purified virions by immunoblotting, immunoprecipitation, and immunoelectron microscopy. It was synthesized as a 120-kDa precursor in infected cells and cleaved into 65-kDa and 55-kDa disulfide-linked subunits close to the time of virion release....

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.78.23.13370-13375.2004

    authors： Lopes FB,Colaco S,May JS,Stevenson PG

    更新日期：2004-12-01 00:00:00

  • Conserved GXXXG- and S/T-like motifs in the transmembrane domains of NS4B protein are required for hepatitis C virus replication.

    abstract：:Hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is an integral membrane protein, which plays an important role in the organization and function of the HCV replication complex (RC). Although much is understood about its amphipathic N-terminal and C-terminal domains, we know very little about the role of the tra...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02298-10

    authors： Han Q,Aligo J,Manna D,Belton K,Chintapalli SV,Hong Y,Patterson RL,van Rossum DB,Konan KV

    更新日期：2011-07-01 00:00:00

  • Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease.

    abstract：UNLABELLED:The emergence of Middle East respiratory syndrome-coronavirus (MERS-CoV) in the Middle East since 2012 has caused more than 900 human infections with ∼40% mortality to date. Animal models are needed for studying pathogenesis and for development of preventive and therapeutic agents against MERS-CoV infection....

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.03427-14

    authors： Agrawal AS,Garron T,Tao X,Peng BH,Wakamiya M,Chan TS,Couch RB,Tseng CT

    更新日期：2015-04-01 00:00:00

  • Systemic immunization with an ALVAC-HIV-1/protein boost vaccine strategy protects rhesus macaques from CD4+ T-cell loss and reduces both systemic and mucosal simian-human immunodeficiency virus SHIVKU2 RNA levels.

    abstract：:Transmission of human immunodeficiency virus type 1 (HIV-1) occurs primarily via the mucosal route, suggesting that HIV-1 vaccines may need to elicit mucosal immune responses. Here, we investigated the immunogenicity and relative efficacy of systemic immunization with two human ALVAC-HIV-1 recombinant vaccines express...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.80.8.3732-3742.2006

    authors： Pal R,Venzon D,Santra S,Kalyanaraman VS,Montefiori DC,Hocker L,Hudacik L,Rose N,Nacsa J,Edghill-Smith Y,Moniuszko M,Hel Z,Belyakov IM,Berzofsky JA,Parks RW,Markham PD,Letvin NL,Tartaglia J,Franchini G

    更新日期：2006-04-01 00:00:00

  • Modification to the capsid of the adenovirus vector that enhances dendritic cell infection and transgene-specific cellular immune responses.

    abstract：:Adenovirus (Ad) gene transfer vectors can be used to transfer and express antigens and function as strong adjuvants and thus are useful platforms for the development of genetic vaccines. Based on the hypothesis that Ad vectors with enhanced infectibility of dendritic cells (DC) may be able to evoke enhanced immune res...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.78.5.2572-2580.2004

    authors： Worgall S,Busch A,Rivara M,Bonnyay D,Leopold PL,Merritt R,Hackett NR,Rovelink PW,Bruder JT,Wickham TJ,Kovesdi I,Crystal RG

    更新日期：2004-03-01 00:00:00

  • Characterization of an extremely basic protein derived from granulosis virus nucleocapsids.

    abstract：:Nucleocapsids were isolated from purified enveloped nucleocapsids of Plodia interpunctella granulosis virus by treatment with Nonidet P-40. When analyzed on sodium dodecyl sulfate-polyacrylamide gels, the nucleocapsids consisted of eight polypeptides. One of these, a major component with a molecular weight of 12,500 (...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.33.2.866-876.1980

    authors： Tweeten KA,Bulla LA,Consigli RA

    更新日期：1980-02-01 00:00:00

  • Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses.

    abstract：:Minute virus of canines (MVC) is a member of the genus Bocavirus in the family Parvoviridae. We have molecularly cloned and sequenced the 5'- and 3'-terminal palindromes of MVC. The MVC genome, 5,404 nucleotides (nt) in length, shared an identity of 52.6% and 52.1% with that of human bocavirus and bovine parvovirus, r...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02569-08

    authors： Sun Y,Chen AY,Cheng F,Guan W,Johnson FB,Qiu J

    更新日期：2009-04-01 00:00:00

  • Extracellular vaccinia virus formation and cell-to-cell virus transmission are prevented by deletion of the gene encoding the 37,000-Dalton outer envelope protein.

    abstract：:There are two types of infectious vaccinia virus particles: intracellular naked virions and extracellular enveloped virions (EEV). To determine the biological role of the enveloped form of vaccinia virus, we produced and characterized a mutant that is defective in EEV formation. The strategy involved replacement by ho...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.65.11.5910-5920.1991

    authors： Blasco R,Moss B

    更新日期：1991-11-01 00:00:00

  • Bacteriophage T4 inhibits colicin E2-induced degradation of Escherichia coli deoxyribonucleic acid. I. Protein synthesis-dependent inhibition.

    abstract：:The deoxyribonucleic acid (DNA) of Escherichia coli B is converted by colicin E2 to products soluble in cold trichloroacetic acid; we show that this DNA degradation (hereafter termed solubilization) is subject to inhibition by infection with bacteriophage T4. At least two modes of inhibition may be differentiated on t...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.8.3.303-310.1971

    authors： Swift RL,Wiberg JS

    更新日期：1971-09-01 00:00:00

  • Adeno-associated virus proteins: origin of the capsid components.

    abstract：:The three primary capsid proteins (A, B, and C) of adeno-associated viruses have been shown previously to contain overlapping amino acid sequences (R. McPherson and J. Rose, J. Virol. 46:523-529, 1983). In the present study we demonstrate definitively that these proteins are encoded in the right half of the adeno-asso...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.52.2.591-597.1984

    authors： Janik JE,Huston MM,Rose JA

    更新日期：1984-11-01 00:00:00

  • Regulation of Sendai virus transcription: evidence for a single promoter in vivo.

    abstract：:The synthesis of Sendai virus RNA species was examined after UV irradiation of cells late in infection. Compared with the inactivation of 50S genomic RNA synthesis, the synthesis of the group of mRNA species that sediments at 18S was inactivated at an average rate consistent with a process of sequential transcription ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.21.3.863-871.1977

    authors： Glazier K,Raghow R,Kingsbury DW

    更新日期：1977-03-01 00:00:00

  • cis-Acting inhibitory elements within the pol-env region of human T-cell leukemia virus type 1 possibly involved in viral persistence.

    abstract：:Human T-cell leukemia virus type 1 (HTLV-1) remains latent throughout the life of the carrier, with cells containing the provirus and viral gene expression efficiently down-regulated. On a molecular level, exactly how viruses are down-regulated in vivo remains unresolved. We described here the possibility that down-re...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.71.6.4485-4494.1997

    authors： Saiga A,Orita S,Minoura-Tada N,Maeda M,Aono Y,Asakawa M,Nakahara K,Kubota R,Osame M,Igarashi H

    更新日期：1997-06-01 00:00:00

  • Accumulation of Cytotoxic CD16+ NK Cells in Simian Immunodeficiency Virus-Infected Lymph Nodes Associated with In Situ Differentiation and Functional Anergy.

    abstract：UNLABELLED:Recent evidence suggests that even in treated infections, human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) replication may continue in lymph nodes (LN), serving as a potential virus reservoir. Here we investigated the effects of lentivirus infection on natural killer (NK) cell frequ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00660-15

    authors： Schafer JL,Li H,Evans TI,Estes JD,Reeves RK

    更新日期：2015-07-01 00:00:00

  • Late domain function identified in the vesicular stomatitis virus M protein by use of rhabdovirus-retrovirus chimeras.

    abstract：:Little is known about the mechanisms used by enveloped viruses to separate themselves from the cell surface at the final step of budding. However, small sequences in the Gag proteins of several retroviruses (L domains) have been implicated in this process. A sequence has been identified in the M proteins of rhabdoviru...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.73.4.3359-3365.1999

    authors： Craven RC,Harty RN,Paragas J,Palese P,Wills JW

    更新日期：1999-04-01 00:00:00

  • Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus.

    abstract：:It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity and led to in vivo attenuation. Pathogenicity was recovered in mutants that also regained E prot...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02158-16

    authors： To J,Surya W,Fung TS,Li Y,Verdià-Bàguena C,Queralt-Martin M,Aguilella VM,Liu DX,Torres J

    更新日期：2017-02-14 00:00:00

  • Identification and Functional Analysis of Novel Nonstructural Proteins of Human Bocavirus 1.

    abstract：UNLABELLED:Human bocavirus 1 (HBoV1) is a single-stranded DNA parvovirus that causes lower respiratory tract infections in young children worldwide. In this study, we identified novel splice acceptor and donor sites, namely, A1' and D1', in the large nonstructural protein (NS1)-encoding region of the HBoV1 precursor mR...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01374-15

    authors： Shen W,Deng X,Zou W,Cheng F,Engelhardt JF,Yan Z,Qiu J

    更新日期：2015-10-01 00:00:00

  • The secondary structure of the human immunodeficiency virus type 1 transcript modulates viral splicing and infectivity.

    abstract：:Splicing of human immunodeficiency virus type 1 (HIV-1) exon 6D is regulated by the presence of a complex splicing regulatory element (SRE) sequence that interacts with the splicing factors hnRNP H and SC35. In this work, we show that, in the context of the wild-type viral sequence, hnRNP H acts as a repressor of exon...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00721-08

    authors： Jablonski JA,Buratti E,Stuani C,Caputi M

    更新日期：2008-08-01 00:00:00

  • Reversal of human cytomegalovirus major immediate-early enhancer/promoter silencing in quiescently infected cells via the cyclic AMP signaling pathway.

    abstract：:The human cytomegalovirus (HCMV) major immediate-early (MIE) enhancer contains five functional cyclic AMP (cAMP) response elements (CRE). Because the CRE in their native context do not contribute appreciably to MIE enhancer/promoter activity in lytically infected human fibroblasts and NTera2 (NT2)-derived neurons, we ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01524-06

    authors： Keller MJ,Wu AW,Andrews JI,McGonagill PW,Tibesar EE,Meier JL

    更新日期：2007-06-01 00:00:00

  • Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 4 (vIRF4) Perturbs the G1-S Cell Cycle Progression via Deregulation of the cyclin D1 Gene.

    abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) infection modulates the host cell cycle to create an environment optimal for its viral-DNA replication during the lytic life cycle. We report here that KSHV vIRF4 targets the β-catenin/CBP cofactor and blocks its occupancy on the cyclin D1 promoter, suppressing the G1-S c...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01897-15

    authors： Lee HR,Mitra J,Lee S,Gao SJ,Oh TK,Kim MH,Ha T,Jung JU

    更新日期：2015-10-21 00:00:00

  • The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A).

    abstract：:The influenza virus NS1 protein inhibits the nuclear export of a spliced viral mRNA, NS2 mRNA (F. V. Alonso-Caplen, M. E. Nemeroff, Y. Qiu, and R. M. Krug, Genes Dev. 6:255-267, 1992). To identify the sequence in NS2 mRNA that is recognized by the NS1 protein, we developed a gel shift assay for the formation of specif...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.68.4.2425-2432.1994

    authors： Qiu Y,Krug RM

    更新日期：1994-04-01 00:00:00

  • CD4 T Cells, CD8 T Cells, and Monocytes Coordinate To Prevent Rift Valley Fever Virus Encephalitis.

    abstract：:Rift Valley fever virus (RVFV) is an arbovirus that causes disease in livestock and humans in Africa and the Middle East. While human disease is typically mild and self-limiting, some individuals develop severe manifestations, such as hepatitis, hemorrhagic fever, or encephalitis. Encephalitis occurs 2 to 3 weeks afte...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01270-18

    authors： Harmon JR,Spengler JR,Coleman-McCray JD,Nichol ST,Spiropoulou CF,McElroy AK

    更新日期：2018-11-27 00:00:00