Abstract:
:It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity and led to in vivo attenuation. Pathogenicity was recovered in mutants that also regained E protein channel activity. In particular, V25F was rapidly compensated by changes at multiple V25F-facing TMD residues located on a neighboring monomer, consistent with a recovery of oligomerization. Here, we show using infected cells that the same mutations, T16A and A26F, in the gamma-CoV infectious bronchitis virus (IBV) lead to, in principle, similar results. However, IBV E A26F did not abolish oligomer formation and was compensated by mutations at N- and C-terminal extramembrane domains (EMDs). The C-terminal EMD mutations clustered along an insertion sequence specific to gamma-CoVs. Nuclear magnetic resonance data are consistent with the presence of only one TMD in IBV E, suggesting that recovery of channel activity and fitness in these IBV E revertant mutants is through an allosteric interaction between EMDs and TMD. The present results are important for the development of IBV live attenuated vaccines when channel-inactivating mutations are introduced in the E protein.IMPORTANCE The ion channel activity of SARS-CoV E protein is a determinant of virulence, and abolishment of channel activity leads to viral attenuation. E deletion may be a strategy for generating live attenuated vaccines but can trigger undesirable compensatory mechanisms through modifications of other viral proteins to regain virulence. Therefore, a more suitable approach may be to introduce small but critical attenuating mutations. For this, the stability of attenuating mutations should be examined to understand the mechanisms of reversion. Here, we show that channel-inactivating mutations of the avian infectious bronchitis virus E protein introduced in a recombinant virus system are deficient in viral release and fitness and that revertant mutations also restored channel activity. Unexpectedly, most of the revertant mutations appeared at extramembrane domains, particularly along an insertion specific for gammacoronaviruses. Our structural data propose a single transmembrane domain in IBV E, suggesting an allosteric interaction between extramembrane and transmembrane domains.
journal_name
J Viroljournal_title
Journal of virologyauthors
To J,Surya W,Fung TS,Li Y,Verdià-Bàguena C,Queralt-Martin M,Aguilella VM,Liu DX,Torres Jdoi
10.1128/JVI.02158-16subject
Has Abstractpub_date
2017-02-14 00:00:00issue
5eissn
0022-538Xissn
1098-5514pii
JVI.02158-16journal_volume
91pub_type
杂志文章abstract::Recent studies have established the presence of human T-cell lymphoma/leukemia virus type I (HTLV-I) in Melanesia. An HTLV-I strain, PNG-1, has now been isolated from a healthy member of the Hagahai, a remote, recently contacted group in Papua New Guinea. To further characterize PNG-1, we employed polymerase chain rea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2556-2563.1992
更新日期:1992-04-01 00:00:00
abstract::Ascoviruses (family Ascoviridae) are double-stranded DNA viruses with circular genomes that attack lepidopterans, where they produce large, enveloped virions, 150 by 400 nm, and cause a chronic, fatal disease with a cytopathology resembling that of apoptosis. After infection, host cell DNA is degraded, the nucleus fra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01639-06
更新日期:2006-12-01 00:00:00
abstract::We previously reported that CD4C/human immunodeficiency virus (HIV)(Nef) transgenic (Tg) mice, expressing Nef in CD4(+) T cells and cells of the macrophage/dendritic cell (DC) lineage, develop a severe AIDS-like disease, characterized by depletion of CD4(+) T cells, as well as lung, heart, and kidney diseases. In orde...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00125-09
更新日期:2009-10-01 00:00:00
abstract::To examine the contribution of the transmembrane envelope glycoprotein (TM) to the infectivity of the human T-cell leukemia virus type 1 (HTLV-1), single amino acid substitutions were introduced throughout its ectodomain. The mutated envelopes were tested for intracellular maturation and for functions, including abili...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7180-7186.1997
更新日期:1997-10-01 00:00:00
abstract:UNLABELLED:The expression of the antiviral host cell factor tetherin is induced by interferon and can inhibit the release of enveloped viruses from infected cells. The Vpu protein of HIV-1 antagonizes the antiviral activity of tetherin, and tetherin antagonists with Vpu-like activity have been identified in other virus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00615-15
更新日期:2015-09-01 00:00:00
abstract:UNLABELLED:A balance between the functions of the influenza virus surface proteins hemagglutinin (HA) and neuraminidase (NA) is thought to be important for the transmission of viruses between humans. Here we describe two pandemic H1N1 viruses, A/swine/Virginia/1814-1/2012 and A/swine/Virginia/1814-2/2012 (pH1N1low-1 an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02537-14
更新日期:2015-06-01 00:00:00
abstract::The retroviral Gag protein is capable of directing the production and release of virus-like particles in the absence of all other viral components. Budding normally occurs after Gag is transported to the plasma membrane by its membrane-targeting and -binding (M) domain. In the Rous sarcoma virus (RSV) Gag protein, the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8692-8699.2000
更新日期:2000-09-01 00:00:00
abstract::The synthesis of closed circular simian virus 40 (SV40) deoxyribonucleic acid (DNA) containing sequences homologous to host cell DNA depends upon the conditions under which the cells are infected. When BS-C-1 monkey cells were infected with non-plaque-purified virus at low multiplicity of infection [MOI, 0.032 plaque-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.2.309-316.1972
更新日期:1972-02-01 00:00:00
abstract::Tryptic peptide maps were prepared for four purified structural proteins derived from several murine leukemia viruses (MuLV's). Analyses of these peptide maps reveal that the p30 proteins of Rauscher, Moloney, and Gross MuLV's are very similar to each other, as are the p10's obtained from these three viruses. In contr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.5.1148-1157.1975
更新日期:1975-05-01 00:00:00
abstract::Understanding the mechanism(s) of action of the hepatitis B virus (HBV)-encoded protein HBx is fundamental to elucidating the underlying mechanisms of chronic liver disease and hepatocellular carcinoma caused by HBV infection. In our continued attempts to identify cellular targets of HBx, we have previously reported t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2840-2846.2000
更新日期:2000-03-01 00:00:00
abstract:UNLABELLED:The disease mechanisms associated with onset and secondary effects of rotavirus (RV) diarrhea remain to be determined and may not be identical. In this study, we investigated whether onset of RV diarrhea is associated with increased intestinal permeability and/or motility. To study the transit time, fluoresc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02927-13
更新日期:2014-03-01 00:00:00
abstract::Assembly of hepatitis delta virus (HDV) in infected human hepatocytes involves association of the 1,679- nucleotide single-stranded genomic RNA (deltaRNA) with multiple copies of both small and large forms of the delta protein (deltaAg) to form a ribonucleoprotein particle which in turn interacts with envelope protein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3709-3719.2002
更新日期:2002-04-01 00:00:00
abstract::Proteomic identification of human papillomavirus type 16 (HPV16) E6-interacting proteins revealed several proteins involved in ubiquitin-mediated proteolysis. In addition to the well-characterized E6AP ubiquitin-protein ligase, a second HECT domain protein (HERC2) and a deubiquitylating enzyme (USP15) were identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00605-09
更新日期:2009-09-01 00:00:00
abstract::We sequenced the leftmost 2,640 base pairs of bacteriophage P4 DNA, thus completing the sequence of the 11,627-base-pair P4 genome. The newly sequenced region encodes three nonessential genes, which are called gop, beta, and cII (in order, from left to right). The gop gene product kills Escherichia coli when the beta ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.24-36.1990
更新日期:1990-01-01 00:00:00
abstract::DNA was extracted from [(3)H]thymidine-labeled Marek's disease virus (MDV) and purified by two cycles of CsCl gradient centrifugation in a fixed-angle rotor. The DNA was transcribed in vitro into (32)P-labeled complementary RNA (cRNA). MDV cRNA did not hybridize with DNA from chicken embryo fibroblast cultures or from...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.4.841-846.1973
更新日期:1973-10-01 00:00:00
abstract::Recombinant adeno-associated virus (rAAV) vectors have been shown to be useful for efficient gene delivery to a variety of dividing and nondividing cells. Mechanisms responsible for the long-term, persistent expression of the rAAV transgene are not well understood. In this study we investigated the kinetics of rAAV-me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3555-3565.2000
更新日期:2000-04-01 00:00:00
abstract::The major immediate-early (MIE) transactivator proteins of cytomegaloviruses (CMV) play a pivotal role in the initiation of virus-host cell interactions. Therefore, cis- and trans-acting factors influencing the expression of these proteins through their upstream promoter-enhancer regions are important determinants of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.516-529.1993
更新日期:1993-01-01 00:00:00
abstract::The Roseobacter clade has been recognized as one of the abundant bacterial lineages in marine environments, which makes the characterization of bacteriophages infecting members of the clade important. Here we report the complete genome sequence of bacteriophage P12053L, which infects Celeribacter sp. strain IMCC12053,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01153-12
更新日期:2012-08-01 00:00:00
abstract:UNLABELLED:An understanding of the antigen-specific B-cell response to the influenza virus hemagglutinin (HA) is critical to the development of universal influenza vaccines, but it has not been possible to examine these cells directly because HA binds to sialic acid (SA) on most cell types. Here, we use structure-based...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03422-13
更新日期:2014-04-01 00:00:00
abstract::Five monoclonal antibodies to the matrix (M) protein of Newcastle disease virus (NDV) Australia-Victoria (AV) strain were generated and characterized. In competitive antibody-binding assays, the antibodies fell into three discrete groups. The antigenic sites described by these antibody groups were designated M1, M2, a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.586-593.1988
更新日期:1988-02-01 00:00:00
abstract::An adenovirus vector encoding murine Fas ligand (mFasL) under an inducible control was derived. In vivo ectopic expression of mFasL in murine livers induced an inflammatory cellular infiltration. Furthermore, ectopic expression of mFasL by myocytes did not allow prolonged vector-mediated transgene expression. Thus, ec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2483-2490.1998
更新日期:1998-03-01 00:00:00
abstract::Papillomaviruses (PVs) are a large family of small DNA viruses infecting mammals, reptiles, and birds. PV infection induces cell proliferation that may lead to the formation of orogenital or skin tumors. PV-induced cell proliferation has been related mainly to the expression of two small oncoproteins, E6 and E7. In ma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01777-08
更新日期:2009-09-01 00:00:00
abstract::Foamy viruses (FVs) are the least known retroviruses commonly found in primates, cats, horses, and cattle. Although FVs are considered apathogenic, simian and feline FVs have been shown to be associated with some transient health abnormalities in animal models. Currently, data regarding the course of infection with bo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02447-12
更新日期:2013-03-01 00:00:00
abstract::Gene expression of human immunodeficiency virus (HIV) is modulated by both cellular transcription factors, which bind to cis-acting regulatory elements in the HIV-1 long terminal repeat (LTR) and the viral transactivator, tat. The enhancer element in the HIV-1 LTR which extends from -103 to -82 is critical for gene ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1002-1009.1994
更新日期:1994-02-01 00:00:00
abstract::Recombinant DNA clones of a viral clone of spleen necrosis virus, an avian retrovirus, were found to have long terminal repeats of different sizes. The variation was in the U3 region of the long terminal repeats, and any one clone had U3 of the same size in both long terminal repeats. The U3 regions in the 5' and 3' l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.163-171.1982
更新日期:1982-01-01 00:00:00
abstract::The DNA of normal chicken embryos contains sequences related to the avian leukosis-sarcoma viruses. RNA-dependent DNA polymerase of these viruses is encoded by a genetic element known as the pol gene. The nature of the endogenous virus pol gene in chicken cells was investigated by testing its ability to participate in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.856-862.1979
更新日期:1979-03-01 00:00:00
abstract::Eleven highly conserved proteins comprise the poxvirus entry-fusion complex (EFC). We focused on vaccinia virus (VACV) O3, a 35-amino acid, largely hydrophobic component of unknown specific function. Experimental evolution was carried out by blindly passaging a virus that was severely impaired in entry due to deletion...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02228-20
更新日期:2021-01-27 00:00:00
abstract::The filoviruses Ebola Zaire virus and Marburg virus are believed to infect target cells through endocytic vesicles, but the details of this pathway are unknown. We used a pseudotyping strategy to investigate the cell biology of filovirus entry. We observed that specific inhibitors of the caveola system, including chol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.5266-5270.2002
更新日期:2002-05-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) vpr inhibits the replication of tumor cell lines and peripheral blood mononuclear cells. Here it is demonstrated that expression of vpr, either in the context of a provirus or from an independent genetic element, induces a discrete cell cycle arrest, with cells containing 4N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6859-6864.1995
更新日期:1995-11-01 00:00:00
abstract::Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the complex cis-acting oriLyt region, which spans nearly 3 kb. DNA synthesis requires six core proteins together with UL84 and IE2. Previously, two essential regions were identified within oriLyt. Essential region I (nucleotides [nt] 92209 to 92573) ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00058-07
更新日期:2007-07-01 00:00:00