Abstract:
UNLABELLED:The disease mechanisms associated with onset and secondary effects of rotavirus (RV) diarrhea remain to be determined and may not be identical. In this study, we investigated whether onset of RV diarrhea is associated with increased intestinal permeability and/or motility. To study the transit time, fluorescent fluorescein isothiocyanate (FITC)-dextran was given to RV-infected adult and infant mice. Intestinal motility was also studied with an opioid receptor agonist (loperamide) and a muscarinic receptor antagonist (atropine). To investigate whether RV increases permeability at the onset of diarrhea, fluorescent 4- and 10-kDa dextran doses were given to infected and noninfected mice, and fluorescence intensity was measured subsequently in serum. RV increased transit time in infant mice. Increased motility was detected at 24 h postinfection (h p.i.) and persisted up to 72 h p.i in pups. Both loperamide and atropine decreased intestinal motility and attenuated diarrhea. Analysis of passage of fluorescent dextran from the intestine into serum indicated unaffected intestinal permeability at the onset of diarrhea (24 to 48 h p.i.). We show that RV-induced diarrhea is associated with increased intestinal motility via an activation of the myenteric nerve plexus, which in turn stimulates muscarinic receptors on intestinal smooth muscles. IMPORTANCE:We show that RV-infected mice have increased intestinal motility at the onset of diarrhea, and that this is not associated with increased intestinal permeability. These new observations will contribute to a better understanding of the mechanisms involved in RV diarrhea.
journal_name
J Viroljournal_title
Journal of virologyauthors
Istrate C,Hagbom M,Vikström E,Magnusson KE,Svensson Ldoi
10.1128/JVI.02927-13subject
Has Abstractpub_date
2014-03-01 00:00:00pages
3161-9issue
6eissn
0022-538Xissn
1098-5514pii
JVI.02927-13journal_volume
88pub_type
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8762-8770.1999
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2083-2090.1993
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pub_type: 杂志文章
doi:10.1128/JVI.66.10.5914-5923.1992
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doi:10.1128/JVI.00266-07
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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doi:10.1128/JVI.78.16.8917-8921.2004
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1400-1407.1991
更新日期:1991-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6357-6364.1993
更新日期:1993-11-01 00:00:00
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journal_title:Journal of virology
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4390-4398.1990
更新日期:1990-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8669-8676.1999
更新日期:1999-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1976-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4729-4736.1996
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abstract::High-risk human papillomaviruses (HPVs) are the causative agents of certain human cancers. HPV type 16 (HPV16) is the papillomavirus most frequently associated with cervical cancer in women. The E6 and E7 genes of HPV are expressed in cells derived from these cancers and can transform cells in tissue culture. Animal e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5887-5893.1999
更新日期:1999-07-01 00:00:00
abstract::The Epstein-Barr virus (EBV) nuclear antigen 3C (EBNA-3C) protein is a transcriptional regulator of viral and cellular genes that is essential for EBV-mediated immortalization of B lymphocytes in vitro. EBNA-3C can inhibit transcription through an association with the cellular DNA-binding protein Jkappa, a function sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5151-5160.2000
更新日期:2000-06-01 00:00:00