Abstract:
:Bovine leukemia virus (BLV) is silent in most cells detectable in vivo, and the repression of its expression allows BLV to evade the host's immune response. In this study, we examined whether CpG methylation of DNA might be involved in the regulation of the expression of BLV in vivo. To investigate the effects of CpG methylation on the activity of the long terminal repeat (LTR) of BLV, we measured the transactivation activity of this region after treatment with the CpG methyltransferase SssI by using a luciferase reporter system. The activity of methylated LTR was significantly lower than that of nonmethylated LTR. Therefore, we examined the extent of CpG methylation of the U3 region and part of the R region of the LTR in BLV-infected cattle and in experimentally BLV-infected sheep at various clinical stages by the bisulfite genomic sequencing method. We detected no or minimal CpG methylation at all stages examined in cattle and sheep, and our results indicate that CpG methylation probably does not participate in the silencing of BLV in vivo.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tajima S,Tsukamoto M,Aida Ydoi
10.1128/jvi.77.7.4423-4430.2003subject
Has Abstractpub_date
2003-04-01 00:00:00pages
4423-30issue
7eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::The purpose of this study was to define the relationship between herpes simplex virus (HSV) latency and in vivo ganglionic reactivation. Groups of mice with numbers of latently infected neurons ranging from 1.9 to 24% were generated by varying the input titer of wild-type HSV type 1 strain 17syn+. Reactivation of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6888-6892.1998
更新日期:1998-08-01 00:00:00
abstract::The long terminal repeat U3 sequences were determined for multiple feline leukemia virus proviruses isolated from naturally occurring T-cell tumors. Heterogeneity was evident, even among proviruses cloned from individual tumors. Proviruses with one, two, or three repeats of the long terminal repeat enhancer sequences ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.4.1675-1682.1990
更新日期:1990-04-01 00:00:00
abstract::The ribonucleic acid (RNA) from Western equine encephalomyelitis (WEE) virions sedimented through sucrose gradients with a sedimentation coefficient of 40S. Another viral RNA which was always associated with infected cells possessed a sedimentation coefficient of 26S. Both 40S and 26S RNA had identical base compositio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.6.558-566.1968
更新日期:1968-06-01 00:00:00
abstract::Hepatitis A virus (HAV) 3C proteinase expressed in Escherichia coli was purified to homogeneity, and its cleavage specificity towards various parts of the viral polyprotein was analyzed. Intermolecular cleavage of the P2-P3 domain of the HAV polyprotein gave rise to proteins 2A, 2B, 2C, 3ABC, and 3D, suggesting that i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1727-1733.1995
更新日期:1995-03-01 00:00:00
abstract::Prior to the introduction of live-attenuated vaccines, mumps virus (MuV) was the leading cause of virus-induced meningitis. Although vaccination has been effective at controlling the disease, the use of insufficiently attenuated strains has been associated with high rates of aseptic meningitis in vaccinees. The molecu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00266-07
更新日期:2007-08-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV/human herpesvirus 8 [HHV-8]) is a gamma-2-herpesvirus responsible for Kaposi's sarcoma as well as primary effusion lymphoma (PEL). KSHV is a lymphotropic virus that has pirated many mammalian genes involved in inflammation, cell cycle control, and angiogenesis. Among these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.57-67.2003
更新日期:2003-01-01 00:00:00
abstract::Monomers of the genomic strand of hepatitis delta virus RNA were transcribed in vitro and then delivered to NIH 3T3 fibroblasts by using a liposome fusion technique. After 7 days, genome replication was detected, but only in fibroblasts that stably expressed the delta antigen. Sequence analysis of the replicated produ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3104-3107.1990
更新日期:1990-06-01 00:00:00
abstract::To analyze the biological activities of the alphavirus glycoproteins, eight different monoclonal antibodies against the two glycoproteins of western equine encephalitis virus were isolated. Five of the eight monoclonal antibodies were shown to be specific for E1 and three for E2 protein by an enzyme-linked immunosorbe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.840-842.1985
更新日期:1985-09-01 00:00:00
abstract::We previously demonstrated that chimeric porcine parvovirus-like particles (PPV:VLP) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong CD4(+) and CD8(+) T-cell responses specific for the foreign epitopes. In the present study, we investigated the immunogenicity...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2739-2744.1999
更新日期:1999-04-01 00:00:00
abstract::HIV-1 buds as an immature, noninfectious virion. Proteolysis of its main structural component, Gag, is required for morphological maturation and infectivity and leads to release of four functional domains and the spacer peptides SP1 and SP2. The N-terminal cleavages of Gag and the separation of SP1 from CA are all ess...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01704-12
更新日期:2012-12-01 00:00:00
abstract::The architecture of a retroviral genome presents some unusual features for transcriptional regulation because of duplication of the transcriptional control sequences in the 5' and 3' long terminal repeats (LTRs). We have studied the transcriptional activity of the 5' and 3' LTRs of human immunodeficiency virus type 1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3830-3840.1994
更新日期:1994-06-01 00:00:00
abstract::Nonconservative substitutions for Tyr-115 in the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) lead to enzymes displaying lower affinity for deoxynucleoside triphosphates (dNTPs) (A. M. Martín-Hernández, E. Domingo, and L. Menéndez-Arias, EMBO J. 15:4434-4442, 1996). Several mutations at th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6293-6298.1999
更新日期:1999-08-01 00:00:00
abstract::F proteins from baculovirus nucleopolyhedrovirus (NPV) group II members are the major budded virus (BV) viral envelope fusion proteins. They undergo furin-like proteolysis processing in order to be functional. F proteins from different baculovirus species have a long cytoplasmic tail domain (CTD), ranging from 48 (Spo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01178-06
更新日期:2006-11-01 00:00:00
abstract::The murine polyomavirus (Py) enters mouse fibroblasts and kidney epithelial cells via an endocytic pathway that is caveola-independent (as well as clathrin-independent). In contrast, uptake of simian virus 40 into the same cells is dependent on caveola. Following the initial uptake of Py, both microtubules and microfi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2615-2622.2003
更新日期:2003-02-01 00:00:00
abstract:UNLABELLED:Arthritogenic alphaviruses such as Ross River virus (RRV) and chikungunya virus (CHIKV) cause large-scale epidemics of severe musculoskeletal disease and have been progressively expanding their global distribution. Since its introduction in July 2014, CHIKV now circulates in the United States. The hallmark o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00224-15
更新日期:2015-08-01 00:00:00
abstract::The beginning of the second century of research in the field of virology (the first virus was discovered in 1898) was marked by its amalgamation with bioinformatics, resulting in the birth of a new domain--viroinformatics. The availability of more than 100 Web servers and databases embracing all or specific viruses (f...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.02027-14
更新日期:2015-02-01 00:00:00
abstract::Gene transfer to differentiated airway epithelia with existing viral vectors is very inefficient when they are applied to the apical surface. This largely reflects the polarized distribution of receptors on the basolateral surface. To identify new receptor-ligand interactions that might be used to redirect vectors to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9234-9239.2000
更新日期:2000-10-01 00:00:00
abstract::The herpes simplex virus type 1 latency-associated transcript (LAT) inhibits apoptosis. We demonstrate here that LAT influences the accumulation of the Bcl-x(L) transcript versus the Bcl-x(S) transcript in Neuro-2A cells. Bcl-x(L) encodes an antiapoptotic protein, whereas Bcl-x(S) encodes a proapoptotic protein. Promo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10714-10718.2003
更新日期:2003-10-01 00:00:00
abstract::Newcastle disease virus (NDV) belongs to serotype 1 of the avian paramyxoviruses (APMV-1) and causes severe disease in chickens. Current live attenuated NDV vaccines are not fully satisfactory. An alternative is to use a viral vector vaccine that infects chickens but does not cause disease. APMV serotype 3 infects a w...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/JVI.00367-11
更新日期:2011-07-01 00:00:00
abstract::The rate-limiting steps in infection by human immunodeficiency virus type 1 (HIV-1) deficient in the viral infectivity factor, Vif, are unknown. As a measurement of completion of the early stages of the HIV-1 life cycle, the levels of viral DNA were examined by polymerase chain reaction amplification during infection ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.6322-6326.1993
更新日期:1993-10-01 00:00:00
abstract::In an earlier study on minus-strand DNA synthesis catalyzed by murine leukemia virus reverse transcriptase, we described a prominent pause site near the polypurine tract (J. Guo, W. Wu, Z. Y. Yuan, K. Post, R. J. Crouch, and J. G . Levin, Biochemistry 34:5018-5029, 1995). We now report that pausing at this site is due...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.7132-7142.1996
更新日期:1996-10-01 00:00:00
abstract::Interferon-induced proteins (IFPs) exert multiple functions corresponding to diverse interferon signals. However, the intracellular functions of many IFPs are not fully characterized. Here, we report that IFP35, a member of the IFP family with a molecular mass of 35 kDa, can interact with the bovine Tas (BTas) regulat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02249-07
更新日期:2008-05-01 00:00:00
abstract::A large number of polyoma virus-transformed cells of rat, mouse, and hamster origin were examined for presence of T-antigen species. The results showed that all lines of cells contained middle and small T antigens, but not all contained a full-sized large T antigen, in some cell lines large T antigen was absent, where...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.219-232.1980
更新日期:1980-07-01 00:00:00
abstract::The replication of vaccinia virus is thought to take place exclusively in the cytoplasm of host cells. However, using DNA-DNA hybridization techniques, it can be shown that a significant fraction of the synthesis of vaccinia DNA takes place in the nucleus as well as the cytoplasm. The (3H) thymiding pulse-labeled vacc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.2.305-315.1975
更新日期:1975-02-01 00:00:00
abstract::Myxomatosis is a recurrent problem on rabbit farms throughout Europe despite the success of vaccines. To identify gene variations of field and vaccine strains that may be responsible for changes in virulence, immunomodulation, and immunoprotection, the genomes of 6 myxoma virus (MYXV) strains were sequenced: German fi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01570-16
更新日期:2017-01-31 00:00:00
abstract::A soluble system was developed that could support DNA replication in simian virus 40 (SV40) chromosomes. DNA synthesis in this system required the presence of purified SV40 large tumor antigen, SV40 chromosomes prepared from virus-infected monkey cells, a crude extract from HeLa cells, and several low-molecular-weight...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4820-4829.1990
更新日期:1990-10-01 00:00:00
abstract::Mounting evidence suggests that human immunodeficiency virus type 1 (HIV-1) Gag-specific T helper cells contribute to effective antiviral control, but their functional characteristics and the precise epitopes targeted by this response remain to be defined. In this study, we generated CD4(+) T-cell clones specific for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9771-9779.2001
更新日期:2001-10-01 00:00:00
abstract::The members of Flaviviridae utilize several endocytic pathways to enter a variety of host cells. Our previous work showed that classical swine fever virus (CSFV) enters porcine kidney (PK-15) cells through a clathrin-dependent pathway that requires Rab5 and Rab7. The entry mechanism for CSFV into other cell lines rema...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00797-18
更新日期:2018-07-17 00:00:00
abstract::We have analyzed three cases of hepatitis C virus (HCV)-infected recipients who received blood from HCV-infected donors. Two recipients were exposed to two different HCV RNA-positive donors, and one was exposed to a single donor. All parental genomes from the actual infecting units of blood and the recipients were def...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.5.2059-2066.2001
更新日期:2001-03-01 00:00:00
abstract::The interrelationship of viral ribonucleic acid (RNA) and protein synthesis in cells infected by Sindbis virus was investigated. When cultures were treated with puromycin early in the course of infection, the synthesis of interjacent RNA (26S) was preferentially inhibited. A similar result was obtained by shifting cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.2.117-122.1969
更新日期:1969-08-01 00:00:00