Intranasal delivery of recombinant parvovirus-like particles elicits cytotoxic T-cell and neutralizing antibody responses.


:We previously demonstrated that chimeric porcine parvovirus-like particles (PPV:VLP) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong CD4(+) and CD8(+) T-cell responses specific for the foreign epitopes. In the present study, we investigated the immunogenicity of PPV:VLP carrying a CD8(+) T-cell epitope from the lymphocytic choriomeningitis virus (LCMV) administered by mucosal routes. Mice immunized intranasally with recombinant PPV:VLP, in the absence of adjuvant, developed high levels of PPV-specific immunoglobulin G (IgG) and/or IgA in their serum, as well as in mucosal sites such as the bronchoalveolar and intestinal fluids. Antibodies in sera from mice immunized parenterally or intranasally with PPV:VLP were strongly neutralizing in vitro. Intranasal immunization with PPV:VLP carrying the LCMV CD8(+) T-cell epitope also elicited a strong peptide-specific cytotoxic-T-cell (CTL) response. In contrast, mice orally immunized with recombinant PPV:VLP did not develop any antibody or CTL responses. We also showed that mice primed with PPV:VLP are still able to develop strong CTL responses after subsequent immunization with chimeric PPV:VLP carrying a foreign CD8(+) T-cell epitope. These results highlight the attractive potential of PPV:VLP as a safe, nonreplicating antigen carrier to stimulate systemic and mucosal immunity after nasal administration.


J Virol


Journal of virology


Sedlik C,Dridi A,Deriaud E,Saron MF,Rueda P,Sarraseca J,Casal JI,Leclerc C




Has Abstract


1999-04-01 00:00:00












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    更新日期:2003-03-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of virology

    pub_type: 杂志文章


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    journal_title:Journal of virology

    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Guan Z,Zhong L,Li C,Wu W,Yuan M,Yang K

    更新日期:2016-03-28 00:00:00

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    pub_type: 杂志文章


    authors: Langeveld JP,Jacobs JG,Hunter N,van Keulen LJ,Lantier F,van Zijderveld FG,Bossers A

    更新日期:2015-10-28 00:00:00