Intranasal delivery of recombinant parvovirus-like particles elicits cytotoxic T-cell and neutralizing antibody responses.

Abstract:

:We previously demonstrated that chimeric porcine parvovirus-like particles (PPV:VLP) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong CD4(+) and CD8(+) T-cell responses specific for the foreign epitopes. In the present study, we investigated the immunogenicity of PPV:VLP carrying a CD8(+) T-cell epitope from the lymphocytic choriomeningitis virus (LCMV) administered by mucosal routes. Mice immunized intranasally with recombinant PPV:VLP, in the absence of adjuvant, developed high levels of PPV-specific immunoglobulin G (IgG) and/or IgA in their serum, as well as in mucosal sites such as the bronchoalveolar and intestinal fluids. Antibodies in sera from mice immunized parenterally or intranasally with PPV:VLP were strongly neutralizing in vitro. Intranasal immunization with PPV:VLP carrying the LCMV CD8(+) T-cell epitope also elicited a strong peptide-specific cytotoxic-T-cell (CTL) response. In contrast, mice orally immunized with recombinant PPV:VLP did not develop any antibody or CTL responses. We also showed that mice primed with PPV:VLP are still able to develop strong CTL responses after subsequent immunization with chimeric PPV:VLP carrying a foreign CD8(+) T-cell epitope. These results highlight the attractive potential of PPV:VLP as a safe, nonreplicating antigen carrier to stimulate systemic and mucosal immunity after nasal administration.

journal_name

J Virol

journal_title

Journal of virology

authors

Sedlik C,Dridi A,Deriaud E,Saron MF,Rueda P,Sarraseca J,Casal JI,Leclerc C

doi

10.1128/JVI.73.4.2739-2744.1999

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

2739-44

issue

4

eissn

0022-538X

issn

1098-5514

journal_volume

73

pub_type

杂志文章
  • The region of the envelope gene of human immunodeficiency virus type 1 responsible for determination of cell tropism.

    abstract::Different isolates of human immunodeficiency virus type 1 (HIV-1) vary in the cell tropisms they display, i.e., the range of cell types in which they are able to establish a productive infection. Here, we report on the phenotypes of recombinants between two molecularly cloned strains of HIV-1. Our results prove that t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.1.305-309.1992

    authors: Cann AJ,Churcher MJ,Boyd M,O'Brien W,Zhao JQ,Zack J,Chen IS

    更新日期:1992-01-01 00:00:00

  • Detection of virus-specific RNA in simian sarcoma-leukemia virus-infected cells in in situ hybridization to viral complementary DNA.

    abstract::An in situ molecular hybridization system which will detect retrovirus RNA in the cytoplasm of individual virus-infected cells has been developed. The technique was applied to cells infected with simian sarcoma-leukemia virus, where the virus-specific RNA was detected by hybridization to simian sarcoma-leukemia virus ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.30.2.637-641.1979

    authors: Kaufman SL,Gallo RC,Miller NR

    更新日期:1979-05-01 00:00:00

  • Broadly Neutralizing Human Immunodeficiency Virus Type 1 Antibody Gene Transfer Protects Nonhuman Primates from Mucosal Simian-Human Immunodeficiency Virus Infection.

    abstract::Broadly neutralizing antibodies (bnAbs) can prevent lentiviral infection in nonhuman primates and may slow the spread of human immunodeficiency virus type 1 (HIV-1). Although protection by passive transfer of human bnAbs has been demonstrated in monkeys, durable expression is essential for its broader use in humans. G...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00908-15

    authors: Saunders KO,Wang L,Joyce MG,Yang ZY,Balazs AB,Cheng C,Ko SY,Kong WP,Rudicell RS,Georgiev IS,Duan L,Foulds KE,Donaldson M,Xu L,Schmidt SD,Todd JP,Baltimore D,Roederer M,Haase AT,Kwong PD,Rao SS,Mascola JR,Nabel

    更新日期:2015-08-01 00:00:00

  • DC-SIGN facilitates fusion of dendritic cells with human T-cell leukemia virus type 1-infected cells.

    abstract::Interactions between the oncogenic retrovirus human T-cell leukemia virus type 1 (HTLV-1) and dendritic cells (DCs) are poorly characterized. We show here that monocyte-derived DCs form syncytia and are infected upon coculture with HTLV-1-infected lymphocytes. We examined the role of DC-specific ICAM-3-grabbing nonint...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.80.10.4771-4780.2006

    authors: Ceccaldi PE,Delebecque F,Prevost MC,Moris A,Abastado JP,Gessain A,Schwartz O,Ozden S

    更新日期:2006-05-01 00:00:00

  • Cultivation and characterization of three strains of murine rotavirus.

    abstract::Three distinct strains of murine rotavirus were adapted to growth in cell culture. These strains are genetically related but not identical; they are serotypically heterogeneous. The cultivatable strains were substantially more infectious (approximately 10(6)-fold) for suckling mice than heterologous simian rotaviruses...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.57.2.585-590.1986

    authors: Greenberg HB,Vo PT,Jones R

    更新日期:1986-02-01 00:00:00

  • Molecular cloning of a family of retroviral sequences found in chimpanzee but not human DNA.

    abstract::A number of retrovirus-like sequences have been cloned from chimpanzee DNA which constitute the chimpanzee homologs of the endogenous colobus type C virus CPC-1. One of the clones contains a nearly complete viral genome, but others have sustained deletions of 1 to 2 kilobases in the polymerase gene. The pattern of rel...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.43.3.914-924.1982

    authors: Bonner TI,Birkenmeier EH,Gonda MA,Mark GE,Searfoss GH,Todaro GJ

    更新日期:1982-09-01 00:00:00

  • Relationship between 3'-azido-3'-deoxythymidine resistance and primer unblocking activity in foscarnet-resistant mutants of human immunodeficiency virus type 1 reverse transcriptase.

    abstract::Phosphonoformate (foscarnet) is a pyrophosphate (PP(i)) analogue and a potent inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), acting through the PP(i) binding site on the enzyme. HIV-1 RT can unblock a chain-terminated DNA primer by phosphorolytic transfer of the terminal residue t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.11.6127-6137.2003

    authors: Meyer PR,Matsuura SE,Zonarich D,Chopra RR,Pendarvis E,Bazmi HZ,Mellors JW,Scott WA

    更新日期:2003-06-01 00:00:00

  • Clade-specific evolution mediated by HLA-B*57/5801 in human immunodeficiency virus type 1 clade A1 p24.

    abstract::HLA-B*57-mediated selection pressure leads to a typical escape pathway in human immunodeficiency virus type 1 (HIV-1) CD8 epitopes such as TW10. Whether this T242N pathway is shared by all clades remains unknown. We therefore assessed the nature of HLA-B*57 selection in a large, observational Kenyan cohort where clade...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01236-09

    authors: McKinnon LR,Capina R,Peters H,Mendoza M,Kimani J,Wachihi C,Kariri A,Kimani M,Richmond M,Kiazyk SK,Fowke KR,Jaoko W,Luo M,Ball TB,Plummer FA

    更新日期:2009-12-01 00:00:00

  • A chimeric vesiculo/alphavirus is an effective alphavirus vaccine.

    abstract::While a large number of mosquito-transmitted alphaviruses are known to cause serious human diseases, there are no licensed vaccines that protect against alphavirus infections. The alphavirus chikungunya virus (CHIKV) has caused multiple recent outbreaks of chikungunya fever. This virus has the potential to cause a wor...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01860-12

    authors: Chattopadhyay A,Wang E,Seymour R,Weaver SC,Rose JK

    更新日期:2013-01-01 00:00:00

  • The human immunodeficiency virus type 1 Vif protein modulates the postpenetration stability of viral nucleoprotein complexes.

    abstract::The vif gene of human immunodeficiency virus type 1 is absolutely required for productive infection of primary cells derived from human blood and certain immortalized T lymphoid cells, for example, H9. Cells with this restrictive phenotype are termed nonpermissive, whereas cell lines in which vif-deficient virus can r...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.8.5297-5305.1996

    authors: Simon JH,Malim MH

    更新日期:1996-08-01 00:00:00

  • Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase.

    abstract::A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Molon...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.38.1.239-248.1981

    authors: Goff S,Traktman P,Baltimore D

    更新日期:1981-04-01 00:00:00

  • Oligomerization and activity of the helicase domain of the tobacco mosaic virus 126- and 183-kilodalton replicase proteins.

    abstract::A protein-protein interaction within the helicase domain of the Tobacco mosaic virus (TMV) 126- and 183-kDa replicase proteins was previously implicated in virus replication (S. Goregaoker, D. Lewandowski, and J. Culver, Virology 282:320-328, 2001). To further characterize the interaction, polypeptides covering the in...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.6.3549-3556.2003

    authors: Goregaoker SP,Culver JN

    更新日期:2003-03-01 00:00:00

  • Modulation of a pore in the capsid of JC polyomavirus reduces infectivity and prevents exposure of the minor capsid proteins.

    abstract:UNLABELLED:JC polyomavirus (JCPyV) infection of immunocompromised individuals results in the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML). The viral capsid of JCPyV is composed primarily of the major capsid protein virus protein 1 (VP1), and pentameric arrangement of VP1 monomers results...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00089-15

    authors: Nelson CD,Ströh LJ,Gee GV,O'Hara BA,Stehle T,Atwood WJ

    更新日期:2015-04-01 00:00:00

  • An assembly defect as a result of an attenuating mutation in the capsid proteins of the poliovirus type 3 vaccine strain.

    abstract::The molecular basis of the temperature-sensitive (ts) phenotype of P3/Sabin, the type 3 vaccine strain of poliovirus, was investigated in light of the known correlation between ts and attenuation phenotypes. A phenylalanine at residue 91 of the capsid protein VP3 was a major determinant of both phenotypes, and attenua...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.65.10.5225-5231.1991

    authors: Macadam AJ,Ferguson G,Arnold C,Minor PD

    更新日期:1991-10-01 00:00:00

  • Complete genome sequence of the novel lytic avian pathogenic coliphage NJ01.

    abstract::Bacteriophages of the C3 morphotype, characterized by very long heads that exceed their width several times, are extremely rare among the Podoviridae family members and constitute only 0.5% of over 5,500 phages that have been examined by the electron microscope (H. W. Ackermann, Arch. Virol. 152:227-243, 2007; H. W. A...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02727-12

    authors: Li Y,Chen M,Tang F,Yao H,Lu C,Zhang W

    更新日期:2012-12-01 00:00:00

  • Segregation of EAV-HP ancient endogenous retroviruses within the chicken population.

    abstract::Avian leukosis virus subgroup J (ALV-J), an exogenous avian retrovirus, is thought to have evolved by recombination with the highly identical env gene of the endogenous avian retrovirus EAV-HP. Embryonic expression of EAV-HP env has been suggested to be associated with the induction of immunological tolerance, a featu...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.23.11935-11938.2001

    authors: Sacco MA,Venugopal K

    更新日期:2001-12-01 00:00:00

  • Antigenic analysis of equine infectious anemia virus (EIAV) variants by using monoclonal antibodies: epitopes of glycoprotein gp90 of EIAV stimulate neutralizing antibodies.

    abstract::Monoclonal antibodies produced against the prototype cell-adapted Wyoming strain of equine infectious anemia virus (EIAV), a lentivirus, were studied for reactivity with the homologous prototype and 16 heterologous isolates. Eighteen hybridomas producing monoclonal antibodies (MAbs) were isolated. Western blot (immuno...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.61.10.2956-2961.1987

    authors: Hussain KA,Issel CJ,Schnorr KL,Rwambo PM,Montelaro RC

    更新日期:1987-10-01 00:00:00

  • Altered central nervous system gene expression caused by congenitally acquired persistent infection with lymphocytic choriomeningitis virus.

    abstract::Neonatal infection of most mouse strains with lymphocytic choriomeningitis virus (LCMV) leads to a life-long persistent infection characterized by high virus loads in the central nervous system (CNS) in the absence of inflammation and tissue destruction. These mice, however, exhibit impaired learning and memory. The o...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00795-06

    authors: Kunz S,Rojek JM,Roberts AJ,McGavern DB,Oldstone MB,de la Torre JC

    更新日期:2006-09-01 00:00:00

  • Melanin or a Melanin-Like Substance Interacts with the N-Terminal Portion of Prion Protein and Inhibits Abnormal Prion Protein Formation in Prion-Infected Cells.

    abstract::Prion diseases are progressive fatal neurodegenerative illnesses caused by the accumulation of transmissible abnormal prion protein (PrP). To find treatments for prion diseases, we searched for substances from natural resources that inhibit abnormal PrP formation in prion-infected cells. We found that high-molecular-w...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01862-16

    authors: Hamanaka T,Nishizawa K,Sakasegawa Y,Oguma A,Teruya K,Kurahashi H,Hara H,Sakaguchi S,Doh-Ura K

    更新日期:2017-02-28 00:00:00

  • Antibody-dependent enhancement of hepatitis C virus infection.

    abstract::Hepatitis C virus (HCV) often causes a persistent infection associated with hypergammaglobulinemia, high levels of antiviral antibody and circulating immune complexes, and immune complex disease. We previously reported that only a limited neutralizing activity to vesicular stomatitis virus or HCV pseudotype is generat...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01867-07

    authors: Meyer K,Ait-Goughoulte M,Keck ZY,Foung S,Ray R

    更新日期:2008-03-01 00:00:00

  • The herpes simplex virus 1 Us11 protein inhibits autophagy through its interaction with the protein kinase PKR.

    abstract::Autophagy is now known to be an essential component of host innate and adaptive immunity. Several herpesviruses have developed various strategies to evade this antiviral host defense. Herpes simplex virus 1 (HSV-1) blocks autophagy in fibroblasts and in neurons, and the ICP34.5 protein is important for the resistance ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01158-12

    authors: Lussignol M,Queval C,Bernet-Camard MF,Cotte-Laffitte J,Beau I,Codogno P,Esclatine A

    更新日期:2013-01-01 00:00:00

  • Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA.

    abstract::The complete nucleotide sequence of the genome of classical swine fever virus (CSFV) strain Alfort/187 was determined from three cDNA libraries constructed by cloning of DNA fragments obtained from independent sets of reverse transcription and PCR. The cDNA fragments were then assembled and inserted downstream of a T7...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.6.3478-3487.1996

    authors: Ruggli N,Tratschin JD,Mittelholzer C,Hofmann MA

    更新日期:1996-06-01 00:00:00

  • Intermonomer Interactions in Hemagglutinin Subunits HA1 and HA2 Affecting Hemagglutinin Stability and Influenza Virus Infectivity.

    abstract:UNLABELLED:Influenza virus hemagglutinin (HA) mediates virus entry by binding to cell surface receptors and fusing the viral and endosomal membranes following uptake by endocytosis. The acidic environment of endosomes triggers a large-scale conformational change in the transmembrane subunit of HA (HA2) involving a loop...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00939-15

    authors: Wang W,DeFeo CJ,Alvarado-Facundo E,Vassell R,Weiss CD

    更新日期:2015-10-01 00:00:00

  • Two forms of VPg on poliovirus RNAs.

    abstract::The protein (VPg) linked to the 5' termini of poliovirus RNAs resolved into two species when subjected to non-equilibrium electrofocusing. The differently charged forms of VPg were not due to protein phosphorylation nor to variability of the number of phosphate residues associated with the nucleotide moiety remaining ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.38.3.863-871.1981

    authors: Richards OC,Hey TD,Ehrenfeld E

    更新日期:1981-06-01 00:00:00

  • The predominant mechanism by which ribavirin exerts its antiviral activity in vitro against flaviviruses and paramyxoviruses is mediated by inhibition of IMP dehydrogenase.

    abstract::It is not yet clear to what extent depletion of intracellular GTP pools contributes to the antiviral activity of ribavirin. Therefore, the antiviral activities of (i) ribavirin, (ii) its 5-ethynyl analogue, 5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide (EICAR), and (iii) mycophenolic acid (MPA) (a compound t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.3.1943-1947.2005

    authors: Leyssen P,Balzarini J,De Clercq E,Neyts J

    更新日期:2005-02-01 00:00:00

  • Downregulation of Poly(ADP-Ribose) Polymerase 1 by a Viral Processivity Factor Facilitates Lytic Replication of Gammaherpesvirus.

    abstract:UNLABELLED:In Kaposi's sarcoma-associated herpesvirus (KSHV), poly(ADP-ribose) polymerase 1 (PARP-1) acts as an inhibitor of lytic replication. Here, we demonstrate that KSHV downregulated PARP-1 upon reactivation. The viral processivity factor of KSHV (PF-8) interacted with PARP-1 and was sufficient to degrade PARP-1 ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00559-15

    authors: Chung WC,Park JH,Kang HR,Song MJ

    更新日期:2015-09-01 00:00:00

  • Use of a murine cytomegalovirus K181-derived bacterial artificial chromosome as a vaccine vector for immunocontraception.

    abstract::Cytomegaloviruses (CMVs) are members of the Betaherpesvirinae subfamily of the Herpesviridae, and their properties of latency, large DNA size, gene redundancy, and ability to be cloned as bacterial artificial chromosomes (BACs) suggest their utility as vaccine vectors. While the K181 strain of murine CMV (MCMV) is wid...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.5.2998-3008.2005

    authors: Redwood AJ,Messerle M,Harvey NL,Hardy CM,Koszinowski UH,Lawson MA,Shellam GR

    更新日期:2005-03-01 00:00:00

  • Characterizing functional domains for TIM-mediated enveloped virus entry.

    abstract:UNLABELLED:T-cell immunoglobulin and mucin domain 1 (TIM-1) and other TIM family members were recently identified as phosphatidylserine (PtdSer)-mediated virus entry-enhancing receptors (PVEERs). These proteins enhance entry of Ebola virus (EBOV) and other viruses by binding PtdSer on the viral envelope, concentrating ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00300-14

    authors: Moller-Tank S,Albritton LM,Rennert PD,Maury W

    更新日期:2014-06-01 00:00:00

  • The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly.

    abstract:UNLABELLED:Baculovirus DNAs are synthesized and inserted into preformed capsids to form nucleocapsids at a site in the infected cell nucleus, termed the virogenic stroma. Nucleocapsid assembly ofAutographa californicamultiple nucleopolyhedrovirus (AcMNPV) requires the major capsid protein VP39 and nine minor capsid pro...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02885-15

    authors: Guan Z,Zhong L,Li C,Wu W,Yuan M,Yang K

    更新日期:2016-03-28 00:00:00

  • Prion Type-Dependent Deposition of PRNP Allelic Products in Heterozygous Sheep.

    abstract:UNLABELLED:Susceptibility or resistance to prion infection in humans and animals depends on single prion protein (PrP) amino acid substitutions in the host, but the agent's modulating role has not been well investigated. Compared to disease incubation times in wild-type homozygous ARQ/ARQ (where each triplet represents...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02316-15

    authors: Langeveld JP,Jacobs JG,Hunter N,van Keulen LJ,Lantier F,van Zijderveld FG,Bossers A

    更新日期:2015-10-28 00:00:00