Abstract:
:The Epstein-Barr virus (EBV) nuclear antigen 3B (EBNA-3B) is considered nonessential for EBV-mediated B-cell growth transformation in vitro based on three virus isolates with EBNA-3B mutations. Two of these isolates could potentially express truncated EBNA-3B products, and, similarly, we now show that the third isolate, IB4, has a point mutation and in-frame deletion of 263 amino acids. In order to test whether a virus with EBNA-3B completely deleted can immortalize B-cell growth, we first cloned the EBV genome as a bacterial artificial chromosome (BAC) and showed that the BAC-derived virus was B-cell immortalization competent. Deletion of the entire EBNA-3B open reading frame from the EBV BAC had no adverse impact on growth of EBV-immortalized B cells, providing formal proof that EBNA-3B is not essential for EBV-mediated B-cell growth transformation in vitro.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chen A,Divisconte M,Jiang X,Quink C,Wang Fdoi
10.1128/JVI.79.7.4506-4509.2005subject
Has Abstractpub_date
2005-04-01 00:00:00pages
4506-9issue
7eissn
0022-538Xissn
1098-5514pii
79/7/4506journal_volume
79pub_type
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