Epstein-Barr virus with the latent infection nuclear antigen 3B completely deleted is still competent for B-cell growth transformation in vitro.

Abstract:

:The Epstein-Barr virus (EBV) nuclear antigen 3B (EBNA-3B) is considered nonessential for EBV-mediated B-cell growth transformation in vitro based on three virus isolates with EBNA-3B mutations. Two of these isolates could potentially express truncated EBNA-3B products, and, similarly, we now show that the third isolate, IB4, has a point mutation and in-frame deletion of 263 amino acids. In order to test whether a virus with EBNA-3B completely deleted can immortalize B-cell growth, we first cloned the EBV genome as a bacterial artificial chromosome (BAC) and showed that the BAC-derived virus was B-cell immortalization competent. Deletion of the entire EBNA-3B open reading frame from the EBV BAC had no adverse impact on growth of EBV-immortalized B cells, providing formal proof that EBNA-3B is not essential for EBV-mediated B-cell growth transformation in vitro.

journal_name

J Virol

journal_title

Journal of virology

authors

Chen A,Divisconte M,Jiang X,Quink C,Wang F

doi

10.1128/JVI.79.7.4506-4509.2005

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

4506-9

issue

7

eissn

0022-538X

issn

1098-5514

pii

79/7/4506

journal_volume

79

pub_type

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