Abstract:
:Hypervariable region 5 (HVR5) is a hydrophilic, serotypically nonconserved loop of the hexon monomer which extrudes from the adenovirus (Ad) capsid. We have replaced the HVR5 sequence of Ad5 with that of heterologous peptides and studied their effects on virus viability and peptide accessibility. A poliovirus model epitope was first inserted in a series of nine "isogenic" viruses that differed in their flanking spacers. Whereas virus productivity was not profoundly altered by any of these modifications, immunoprecipitation experiments under nondenaturing conditions demonstrated that epitope recognition by its cognate monoclonal antibody (C3 MAb) was strongly linker dependent and correlated perfectly with the ability of C3 MAb to inhibit transgene delivery and expression. An alphav-specific ligand (DCRGDCF) was then inserted in a suitable linker context to investigate whether hexon-modified capsids would enhance the transduction of cells displaying limiting amounts of the virus attachment receptors. Interestingly, although hexon has never been implicated in Ad entry, the modified virus significantly increased the transduction of human vascular smooth muscle cells in vitro. Competition experiments with 293 cells saturated with recombinant knob further indicated that the hexon-modified virus could use an additional, knob-independent pathway for entry. We concluded that genetic engineering of the Ad5 hexon monomer constitutes a novel and feasible approach to equip the virus with additional targeting ligands.
journal_name
J Viroljournal_title
Journal of virologyauthors
Vigne E,Mahfouz I,Dedieu JF,Brie A,Perricaudet M,Yeh Pdoi
10.1128/JVI.73.6.5156-5161.1999subject
Has Abstractpub_date
1999-06-01 00:00:00pages
5156-61issue
6eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::Mixed infection of a cell by vesicular stomatitis virus (VSV) and retroviruses results in the production of progeny virions bearing the genome of one virus encapsidated by the envelope proteins of the other. The mechanism for the phenomenon of pseudotype formation is not clear, although specific recognition of a viral...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1202-1207.1991
更新日期:1991-03-01 00:00:00
abstract::We followed the dynamics of capsid amino acid replacement among 403 Nigerian outbreak isolates of type 2 circulating vaccine-derived poliovirus (cVDPV2) from 2005 through 2011. Four different functional domains were analyzed: (i) neutralizing antigenic (NAg) sites, (ii) residues binding the poliovirus receptor (PVR), ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01949-17
更新日期:2018-04-13 00:00:00
abstract::Oncornavirus 60 to 70S ribonucleic acids (RNA), such as those from avian myeloblastosis virus, Schmidt-Ruppin virus, or mouse sarcoma-mouse leukemia viruses, isolated by conventional techniques, contain 4S transferlike RNA molecules that are released upon dissociation of the 60 to 70S RNA with heat. The 4S RNA represe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.2.254-256.1971
更新日期:1971-08-01 00:00:00
abstract::A number of human immunodeficiency type 1 (HIV-1)-based vectors have recently been shown to transduce nondividing cells in vivo as well as in vitro. However, if these vectors are to be considered for eventual clinical use, a major consideration is to reduce the probability of unintended generation of replication-compe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1828-1834.1999
更新日期:1999-03-01 00:00:00
abstract::Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.11890-11903.2004
更新日期:2004-11-01 00:00:00
abstract:UNLABELLED:Coronaviruses (CoVs) are unique in encoding a 3'→5' exoribonuclease within nonstructural protein 14 (nsp14-ExoN) that is required for high-fidelity replication, likely via proofreading. nsp14 associates with the CoV RNA-dependent RNA polymerase (nsp12-RdRp), and nsp14-ExoN activity is enhanced by binding nsp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00110-15
更新日期:2015-06-01 00:00:00
abstract::Reassortant viruses which possessed the hemagglutinin and neuraminidase genes of wild-type human influenza A viruses and the remaining six RNA segments (internal genes) of the avian A/Pintail/Alberta/119/79 (H4N6) virus were previously found to be attenuated in humans. To study the genetic basis of this attenuation, w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2857-2863.1987
更新日期:1987-09-01 00:00:00
abstract::The genome of a human papillomavirus (HPV) found in a patient with Bowen's disease of the skin was molecularly cloned. Blot hybridization experiments, performed under stringent conditions, revealed no cross-hybridization between this HPV DNA and the other known HPV DNAs, showing that this HPV represents a new type, te...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.2.688-692.1986
更新日期:1986-02-01 00:00:00
abstract::Defective interfering (DI) RNAs of Tomato bushy stunt virus (TBSV), a plus-sense RNA virus, comprise four conserved noncontiguous regions (I through IV) derived from the viral genome. Region III, a 70-nucleotide-long sequence corresponding to a genomic segment located 378 nucleotides upstream of the 3' terminus of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.245-257.2003
更新日期:2003-01-01 00:00:00
abstract::Some animal rotaviruses require the presence of sialic acid (SA) on the cell surface to infect the cell. We have isolated variants of rhesus rotavirus (RRV) whose infectivity no longer depends on SA. Both the SA-dependent and -independent interactions of these viruses with the cell are mediated by the virus spike prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.593-599.2000
更新日期:2000-01-01 00:00:00
abstract:UNLABELLED:CD4+ T cells play a pivotal role in the control of chronic viral infections. Recently, nontraditional CD4+ T cell functions beyond helper effects have been described, and a role for cytolytic CD4+ T cells in the control of HIV infection has been suggested. We define here the transcriptional, phenotypic, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00438-15
更新日期:2015-08-01 00:00:00
abstract::Coinfection with HIV-1 and Kaposi's sarcoma-associated herpesvirus (KSHV) often leads to AIDS-related malignancies, including Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). The interaction between HIV and KSHV plays a pivotal role in the progression of these malignancies. We have previously demonstrated th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00580-18
更新日期:2018-08-29 00:00:00
abstract::Translation of early RNA specific for the leftmost early region 1 of adenovirus type 5 DNA in a rabbit reticulocyte cell-free system resulted in the synthesis of proteins with the following molecular weights: 65,000 (65K), 54K, 42 to 34K, 29K, 25K, 19K, and 18K. All of these proteins could be immunoprecipitated with h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.524-529.1981
更新日期:1981-01-01 00:00:00
abstract::The poliovirus-encoded, membrane-associated VPg-precursor polypeptide 3AB has been implicated in the initiation of viral RNA synthesis. We have expressed 3AB and 3A polypeptides in eukaryotic cells and examined their localization using indirect immunofluorescence and a direct in vitro membrane-binding assay. Results p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4468-4477.1994
更新日期:1994-07-01 00:00:00
abstract::Adeno-associated virus (AAV) is a human parvovirus currently being developed as a vector for gene therapy applications. Because the gene transfer vector commonly retains only the AAV terminal repeats, propagation of recombinant AAV (rAAV) requires that the viral replication (Rep) and capsid (Cap) proteins be supplied ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1897-1905.1997
更新日期:1997-03-01 00:00:00
abstract::Although the etiology of multiple sclerosis (MS) is not known, several factors play a role in this disease: genetic contributions, immunologic elements, and environmental factors. Viruses and virus infections have been associated with the initiation and/or enhancement of exacerbations in MS. Theiler's murine encephalo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.993-1000.1999
更新日期:1999-02-01 00:00:00
abstract::Interactions between the oncogenic retrovirus human T-cell leukemia virus type 1 (HTLV-1) and dendritic cells (DCs) are poorly characterized. We show here that monocyte-derived DCs form syncytia and are infected upon coculture with HTLV-1-infected lymphocytes. We examined the role of DC-specific ICAM-3-grabbing nonint...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.10.4771-4780.2006
更新日期:2006-05-01 00:00:00
abstract::The genome of the human immunodeficiency virus type 1 (HIV-1) is highly heterogeneous. Some of this genomic variability is reflected in the biologic and serologic differences observed among various strains of HIV-1. To map the viral determinants that correlate with pathogenicity of the virus, recombinant viruses were ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4390-4398.1990
更新日期:1990-09-01 00:00:00
abstract:UNLABELLED:PML is the organizer of cellular structures termed nuclear domain 10 (ND10) or PML-nuclear bodies (PML-NBs) that act as key mediators of intrinsic immunity against human cytomegalovirus (HCMV) and other viruses. The antiviral function of ND10 is antagonized by viral regulatory proteins such as the immediate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01973-15
更新日期:2015-11-11 00:00:00
abstract::Originally identified as the gamma interferon-inducing factor, interleukin-18 (IL-18) was rediscovered as a proinflammatory cytokine related to the IL-1 family of cytokines that plays an important role in both innate and adaptive immune responses against viruses and intracellular pathogens. Despite its importance in i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.12448-12456.2002
更新日期:2002-12-01 00:00:00
abstract::Murine leukemia viruses isolated from radiation-induced BALB/c leukemias were characterized with respect to viral proteins and RNA. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the viral structural proteins revealed that for p12, p15, p30, and gp70, three of four electrophoretic variants of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.661-670.1980
更新日期:1980-02-01 00:00:00
abstract::The use of adenovirus vectors for human gene therapy is limited by potent inflammatory responses that result in significant morbidity. In kidney-derived epithelial cells (REC), activation of extracellular signal-regulated kinase 1/2 (ERK) and p38 kinase (p38) pathways occurred within 20 min of transduction with the se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1559-1568.2002
更新日期:2002-02-01 00:00:00
abstract::Radioactive DNA complementary to nucleotide sequences in Moloney murine sarcoma virus (MSV) and Moloney leukemia virus (M-MuLV) complex was made by the endogenous reverse transcriptase reaction. These virus stocks contained a threefold excess of MSV over M-MuLV as measured by biological assay. The complementary DNA wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.481-490.1976
更新日期:1976-05-01 00:00:00
abstract:UNLABELLED:Following retrovirus entry, the viral capsid (CA) disassembles into its component capsid proteins. The rate of this uncoating process, which is regulated by CA-CA interactions and by the association of the capsid with host cell factors like cyclophilin A (CypA), can influence the efficiency of reverse transc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01388-14
更新日期:2014-09-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV) infects a number of different cell types, including mammary gland and lymphoid cells, in vivo. To identify the cellular receptor for this virus, a mouse cDNA expression library was transfected into Cos-7 monkey kidney cells, and those transfected cells able to bind virus were selected ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3066-3071.1998
更新日期:1998-04-01 00:00:00
abstract::Mutant recombinant respiratory syncytial viruses (RSV) which cannot express the NS1 and M2-2 proteins, designated rA2DeltaNS1 and rA2DeltaM2-2, respectively, were evaluated as live-attenuated RSV vaccines. The rA2DeltaNS1 virus contains a large deletion that should have the advantageous property of genetic stability d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9317-9321.2000
更新日期:2000-10-01 00:00:00
abstract::The 1957 A/H2N2 influenza virus caused an estimated 2 million fatalities during the pandemic. Since viruses of the H2 subtype continue to infect avian species and pigs, the threat of reintroduction into humans remains. To determine factors involved in the zoonotic origin of the 1957 pandemic, we performed analyses on ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02590-14
更新日期:2015-02-01 00:00:00
abstract::After entering a host cell, retroviruses such as simian immunodeficiency virus (SIV) uncoat, disassembling the viral capsid. Rates of uncoating that are too high and too low can be detrimental to the efficiency of infection. Rapid uncoating typically leads to blocks in reverse transcription, but the basis for replicat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03239-12
更新日期:2013-04-01 00:00:00
abstract::When human A431 cells, which carry high numbers of epidermal growth factor (EGF) receptors, are exposed to EGF, the total content of phosphotyrosine in cell protein is increased, the EGF receptor becomes phosphorylated at tyrosine, and new phosphotyrosine-containing 36,000- and 81,000-dalton proteins are detected. We ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.3.752-764.1983
更新日期:1983-12-01 00:00:00
abstract::ICP0 is a potent activator of herpes simplex virus type 1 gene expression in transient assays and in productive infection. A role for ICP0 in reactivation from latency in vivo has also been suggested on the basis of the observation that viruses with mutations in both copies of the diploid gene for ICP0 reactivate less...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7501-7512.1993
更新日期:1993-12-01 00:00:00