Abstract:
:Interactions between the oncogenic retrovirus human T-cell leukemia virus type 1 (HTLV-1) and dendritic cells (DCs) are poorly characterized. We show here that monocyte-derived DCs form syncytia and are infected upon coculture with HTLV-1-infected lymphocytes. We examined the role of DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN), a C-type lectin expressed in DCs, in HTLV-1-induced syncytium formation. DC-SIGN is known to bind with high affinity to various viral envelope glycoproteins, including human immunodeficiency virus (HIV) and hepatitis C virus, as well as to the cellular receptors ICAM-2 and ICAM-3. After cocultivating DCs and HTLV-1-infected cells, we found that anti-DC-SIGN monoclonal antibodies (MAbs) were able to decrease the number and size of HTLV-1-induced syncytia. Moreover, expression of the lectin in epithelial-cell lines dramatically enhanced the ability to fuse with HTLV-1-positive cells. Interestingly, in contrast to the envelope (Env) glycoproteins of HIV and other viruses, that of HTLV-1 does not bind directly to DC-SIGN. The facilitating role of the lectin in HTLV-1 syncytium formation is mediated by its interaction with ICAM-2 and ICAM-3, as demonstrated by use of MAbs directed against these adhesion molecules. Altogether, our results indicate that DC-SIGN facilitates HTLV-1 infection and fusion of DCs through an ICAM-dependent mechanism.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ceccaldi PE,Delebecque F,Prevost MC,Moris A,Abastado JP,Gessain A,Schwartz O,Ozden Sdoi
10.1128/JVI.80.10.4771-4780.2006subject
Has Abstractpub_date
2006-05-01 00:00:00pages
4771-80issue
10eissn
0022-538Xissn
1098-5514pii
80/10/4771journal_volume
80pub_type
杂志文章abstract::Rabies, caused by rabies virus (RABV), is a fatal encephalitis in humans and other mammals, which continues to present a public health threat in most parts of the world. Our previous study demonstrated that Toll-like receptor 7 (TLR7) is essential in the induction of anti-RABV antibodies via the facilitation of germin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00111-20
更新日期:2020-04-16 00:00:00
abstract::Enviroxime is an antienterovirus compound that targets viral protein 3A and/or 3AB and suppresses a step in enterovirus replication by unknown mechanism. To date, four antienterovirus compounds, i.e., GW5074, Flt3 inhibitor II, TTP-8307, and AN-12-H5, are known to have similar mutations in the 3A protein-encoding regi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02249-10
更新日期:2011-03-01 00:00:00
abstract::Several reports have shown that human cytomegalovirus (HCMV)-infected cells are resistant to NK lysis. These studies have focused on receptor-ligand interactions, and different HCMV proteins have been indicated to mediate inhibitory NK signals. Here, we report that the HCMV protein UL16 is of major importance for the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4539-4545.2003
更新日期:2003-04-01 00:00:00
abstract::Arenaviruses are rodent-borne viruses with a bisegmented RNA genome. A genetically unique arenavirus, Lujo virus, was recently discovered as the causal agent of a nosocomial outbreak of acute febrile illness with hemorrhagic manifestations in Zambia and South Africa. The outbreak had a case fatality rate of 80%. A rev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01144-12
更新日期:2012-10-01 00:00:00
abstract::Glycoprotein gIII of pseudorabies virus is a major antigen found in the envelopes of virus particles as well as in and on the surfaces of infected cells. It is not an essential gene product for virus growth in tissue culture. In this report, we provide evidence that, although it is not essential, the gIII protein is r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2512-2515.1988
更新日期:1988-07-01 00:00:00
abstract::There is an incomplete understanding of the differences between neonatal immune responses that contribute to the increased susceptibility of neonates to some viral infections. We tested the hypothesis that neonates are more susceptible than adults to mouse adenovirus type 1 (MAV-1) respiratory infection and are impair...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06967-11
更新日期:2012-04-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) typically displays two different phases in its life cycle, the default latent phase and the lytic phase. There is a short period of lytic gene expression in the early stage of KSHV primary infection. The factors involved in the shutdown process of lytic gene exp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00596-14
更新日期:2014-07-01 00:00:00
abstract::Defensins are innate immune effector peptides expressed at mucosal surfaces throughout the human body and are potently antiviral in vitro The role of defensins in viral pathogenesis in vivo is poorly understood; however, recent studies have revealed that defensin-virus interactions in vivo are complicated and distinct...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00904-15
更新日期:2016-05-12 00:00:00
abstract::The sensitivity of highly purified human fibroblast interferon and partially purified human leukocyte interferon to several proteolytic and glycolytic enzymes was determined with respect to antiviral activity, isoelectric point, molecular weight, and thermal stability. Leucine aminopeptidase altered the distribution o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.390-399.1980
更新日期:1980-08-01 00:00:00
abstract::Previous in vitro analyses have shown that the human immunodeficiency virus type 1 (HIV-1) integrase uses either manganese or magnesium to assemble as a stable complex on the donor substrate and to catalyze strand transfer. We now demonstrate that subsequent to assembly, catalysis of both 3' end processing and strand ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.7005-7011.1997
更新日期:1997-09-01 00:00:00
abstract::Two essential gene products of herpes simplex virus type 1, the viral DNA polymerase (pol) and UL42, its accessory protein, physically and functionally interact to form the core of the viral DNA replication complex. Understanding this essential interaction would provide a basis from which to develop novel anti-herpesv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.5922-5931.1993
更新日期:1993-10-01 00:00:00
abstract::We examined cellular components which associate with p40tax, the viral transactivation molecule of human T-cell leukemia virus type I. Such molecules were searched by immunoprecipitation with polyclonal and monoclonal antibodies specific for p40tax. Two cellular proteins with molecular masses of 95 kDa (p95) and 60 kD...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.1040-1049.1992
更新日期:1992-02-01 00:00:00
abstract::The promoter P7535 of human papillomavirus type 8 and the promoter P7185 of bovine papillomavirus type 1 are negatively regulated by viral E2 proteins via the promoter proximal binding sites P2 and BS1, respectively. Mutations of these E2 binding sites can reduce basal promoter activity. This suggests binding of a tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.8029-8034.1997
更新日期:1997-10-01 00:00:00
abstract::Extracts made from human respiratory syncytial virus (RSV)-infected Hep-2 cells synthesized mRNAs encoded by all known viral genes. In contrast, RSV ribonucleoproteins purified from infected cells failed to transcribe in vitro; transcription was restored by addition of a cytoplasmic extract of uninfected Hep-2 cells, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6813-6818.1992
更新日期:1992-11-01 00:00:00
abstract::The limited efficiency of in vivo gene transfer by replication-deficient retroviral vectors remains an obstacle to achieving effective gene therapy for solid tumors. One approach to circumvent this problem is the use of replication-competent retroviral vectors. However, the application of such vectors is at a comparat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02470-06
更新日期:2007-07-01 00:00:00
abstract::Visna lentiviruses have a natural tropism for cells of the macrophage lineage of sheep and goats, but virus replication in these cells in vivo is restricted so that only small quantities of virus are produced. One restricting factor suggested in previous studies is that virus replication is dependent on the maturity o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.1.67-74.1986
更新日期:1986-04-01 00:00:00
abstract::Dendritic cells (DC) are considered to be important contributors to human immunodeficiency virus type 1 (HIV-1) transmission and pathogenesis. As the first target cells in mucosal tissues, they can be become productively infected and can also capture virions and transfer them efficiently to CD4(+) T cells located with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01420-08
更新日期:2009-03-01 00:00:00
abstract::The VP6 protein of bluetongue virus possesses a number of activities, including nucleoside triphosphatase, RNA binding, and helicase activity (N. Stauber, J. Martinez-Costas, G. Sutton, K. Monastyrskaya, and P. Roy, J. Virol. 71:7220-7226, 1997). Although the enzymatic functions of the protein have been documented, a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11347-11356.2003
更新日期:2003-11-01 00:00:00
abstract::African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal viral disease of domestic pigs that has significant economic consequences for the swine industry. The control of African swine fever (ASF) has been hampered by the unavailability of vaccines. Successful experimental vaccines hav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01760-16
更新日期:2016-12-16 00:00:00
abstract::The replication of vaccinia virus is thought to take place exclusively in the cytoplasm of host cells. However, using DNA-DNA hybridization techniques, it can be shown that a significant fraction of the synthesis of vaccinia DNA takes place in the nucleus as well as the cytoplasm. The (3H) thymiding pulse-labeled vacc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.2.305-315.1975
更新日期:1975-02-01 00:00:00
abstract::We have investigated the molecular basis of biological differences observed among cell line-adapted isolates of the human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) and the simian immunodeficiency virus (SIV) in response to receptor binding by using a soluble form of CD4 (sCD4) as a receptor mimic. We find...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7383-7393.1993
更新日期:1993-12-01 00:00:00
abstract::Human CD317 (BST-2/tetherin) is an intrinsic immunity factor that blocks the release of retroviruses, filoviruses, herpesviruses, and arenaviruses. It is unclear whether CD317 expressed endogenously in rodent cells has the capacity to interfere with the replication of the retroviral rodent pathogen murine leukemia vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01067-10
更新日期:2010-11-01 00:00:00
abstract::E26 is a replication-defective avian retrovirus that causes an erythroblastic leukemia in vivo and transforms hematopoietic precursor cells of both the erythroid and the myeloid lineages in vitro. The E26 genome contains two sets of cell-derived sequences, ets and myb. myb sequences are also present in avian myeloblas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.398-402.1989
更新日期:1989-01-01 00:00:00
abstract::Human herpesvirus 6 (HHV-6)-specific monoclonal antibody (Mab) 9A5D12 reacted with the nucleus of HHV-6 strain GS-infected cells and immunoprecipitated a phosphorylated polypeptide with an approximate size of 41 kDa, designated HHV-6 P41. A 110-kDa polypeptide was also immunoprecipitated by the MAb. These polypeptides...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.2884-2894.1991
更新日期:1991-06-01 00:00:00
abstract:UNLABELLED:In a genome-wide small interfering RNA (siRNA) screen, we recently identified the interferon (IFN)-inducible protein 35 (IFI35; also known as IFP35) as a factor required for vesicular stomatitis virus (VSV) infection. Studies reported here were conducted to further understand the role and requirement of IFI3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03202-13
更新日期:2014-03-01 00:00:00
abstract::Alfalfa mosaic virus (AMV) and ilarvirus RNAs are infectious only in the presence of the viral coat protein; therefore, an understanding of coat protein's function is important for defining viral replication mechanisms. Based on in vitro replication experiments, the conformational switch model states that AMV coat pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5752-5761.2005
更新日期:2005-05-01 00:00:00
abstract::Bacteriophages of the C3 morphotype, characterized by very long heads that exceed their width several times, are extremely rare among the Podoviridae family members and constitute only 0.5% of over 5,500 phages that have been examined by the electron microscope (H. W. Ackermann, Arch. Virol. 152:227-243, 2007; H. W. A...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02727-12
更新日期:2012-12-01 00:00:00
abstract::Reverse transcription in hepatitis B viruses is initiated through a unique protein priming mechanism whereby the viral reverse transcriptase (RT) first assembles into a ribonucleoprotein (RNP) complex with its RNA template and then initiates DNA synthesis de novo using the RT itself as a protein primer. RNP formation ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11447-11455.2000
更新日期:2000-12-01 00:00:00
abstract::HA20, a mutant of Bacillus subtilis phage SPO1, is deficient in both DNA replication and late transcription. HA20 contains mutations in two different genes, which suggested that the two effects might be caused independently. However, single-mutation derivatives, affected only in gene 27, were deficient for both activi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.300-301.1984
更新日期:1984-01-01 00:00:00
abstract::Sequences present at the genomic termini of herpesviruses become linked during lytic-phase replication and provide the substrate for cleavage and packaging of unit length viral genomes. We have previously shown that homologs of the consensus herpesvirus cleavage-packaging signals, pac1 and pac2, are located at the lef...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.320-329.1998
更新日期:1998-01-01 00:00:00