Abstract:
:Adeno-associated virus (AAV) is a human parvovirus currently being developed as a vector for gene therapy applications. Because the gene transfer vector commonly retains only the AAV terminal repeats, propagation of recombinant AAV (rAAV) requires that the viral replication (Rep) and capsid (Cap) proteins be supplied in trans. In an effort to optimize the production of these vectors, a panel of helper plasmids was constructed to determine if expression of the rep and/or cap genes is a limiting factor for rAAV packaging. Expression of the Rep and Cap proteins was increased by replacing the endogenous AAV promoters, p5 and p40, with the Rous sarcoma virus (RSV) long terminal repeat (LTR) and the cytomegalovirus immediate-early promoter, respectively. Increased synthesis of the Cap proteins resulted in an approximately 10-fold increase in the yield of rAAV, indicating that production of capsid proteins is one limiting factor for rAAV packaging. Expression of the rep gene from the RSV LTR not only failed to increase the yield of rAAV but also prevented activation of p40 transcription with adenovirus infection, resulting in a reduced level of capsid protein synthesis.
journal_name
J Viroljournal_title
Journal of virologyauthors
Vincent KA,Piraino ST,Wadsworth SCdoi
10.1128/JVI.71.3.1897-1905.1997subject
Has Abstractpub_date
1997-03-01 00:00:00pages
1897-905issue
3eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::Current adeno-associated virus (AAV) gene therapy vectors package a transgene flanked by the terminal repeats (TRs) of AAV type 2 (AAV2). Although these vectors are replication deficient, wild-type (wt) AAV2 prevalent in the human population could lead to replication and packaging of a type 2 TR (TR2)-flanked transgen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02466-08
更新日期:2009-04-01 00:00:00
abstract::Inhibition of host-directed gene expression by the matrix (M) protein of vesicular stomatitis virus (VSV) effectively blocks host antiviral responses, promotes virus replication, and disables the host cell. However, dendritic cells (DC) have the capacity to resist these effects and remain functional during VSV infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00680-13
更新日期:2013-11-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) alkaline nuclease, encoded by the UL12 gene, plays an important role in HSV-1 replication, as a UL12 null mutant displays a severe growth defect. The HSV-1 alkaline exonuclease UL12 interacts with the viral single-stranded DNA binding protein ICP8 and promotes strand exchange in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01506-10
更新日期:2010-12-01 00:00:00
abstract::The retroviral Gag precursor plays an important role in the assembly of virion particles. The capsid (CA) protein of the Gag molecule makes a major contribution to this process. In the crystal structure of the free CA protein of the human immunodeficiency virus type 1 (HIV-1), 11 residues of the C terminus were found ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9675-9688.2004
更新日期:2004-09-01 00:00:00
abstract::The identification of several simian immunodeficiency virus mac251 (SIV(mac251)) cytotoxic T-lymphocyte epitopes recognized by CD8(+) T cells of infected rhesus macaques carrying the Mamu-A*01 molecule and the use of peptide-major histocompatibility complex tetrameric complexes enable the study of the frequency, bread...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11483-11495.2001
更新日期:2001-12-01 00:00:00
abstract::Analyses of prototype vesicular stomatitis (VSV, Indiana serotype) mRNA-32P-labeled viral RNA duplexes have established the assignments of 65 of the 72 large oligonucleotides that are recovered by two-dimensional electrophoresis of RNase T1 digests of the viral RNA. Fifty of the oligonucleotides are recovered in the L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.116-123.1979
更新日期:1979-04-01 00:00:00
abstract::Gammaherpesviruses are important human and animal pathogens. Infection control has proven difficult because the key process of transmission is ill understood. Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus of mice, is transmitted sexually. We show that this depends on the major virion envelope glycoprotein gp150. gp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00235-17
更新日期:2017-06-09 00:00:00
abstract::Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has caused a pandemic of historic proportions and continues to spread globally, with enormous consequences to human health. Currently there is no vaccine, effective therapeutic, or prophylactic. As with other betacoronaviruses, attachment and entry o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01987-20
更新日期:2021-01-13 00:00:00
abstract::Baculoviruses, although they infect insects in nature, can transduce a wide variety of mammalian cells and are therefore promising gene therapy vectors. However, baculovirus transduction into many mammalian cells is very inefficient, and the limiting stages and factors remain unknown. An important finding is that a sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00709-19
更新日期:2019-07-17 00:00:00
abstract::The 55-kDa E1B protein of adenovirus, which binds to and inactivates the tumor suppressor protein p53, is not expressed in the adenoviral mutant termed ONYX-015 (i.e., dl1520). It was reported that the mutant virus due to a deletion in E1B is able to replicate only in cells deficient for wild-type p53. Accordingly, dl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9470-9478.1998
更新日期:1998-12-01 00:00:00
abstract::Recombinant strains of replication-competent rhesus monkey rhadinovirus (RRV) were constructed in which strong promoter/enhancer elements were used to drive expression of simian immunodeficiency virus (SIV) Env or Gag or a Rev-Tat-Nef fusion protein. Cultured rhesus monkey fibroblasts infected with each recombinant st...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00865-11
更新日期:2011-12-01 00:00:00
abstract::The synthesis of membrane protein after infection with bacteriophage T4 was examined. Protein constituents of both the cytoplasmic and outer membrane are made during the infective cycle. In addition, newly synthesized membrane protein is found in material which has a buoyant density greater than that of either of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.73-80.1974
更新日期:1974-01-01 00:00:00
abstract::Understanding the mechanism(s) of action of the hepatitis B virus (HBV)-encoded protein HBx is fundamental to elucidating the underlying mechanisms of chronic liver disease and hepatocellular carcinoma caused by HBV infection. In our continued attempts to identify cellular targets of HBx, we have previously reported t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2840-2846.2000
更新日期:2000-03-01 00:00:00
abstract::The human papillomavirus (HPV) type 16 (HPV16) E6 protein can stimulate mechanistic target of rapamycin complex 1 (mTORC1) signaling and cap-dependent translation through activation of the PDK1 and mTORC2 kinases. Here we report that HPV18 E6 also enhances cap-dependent translation. The integrity of LXXLL and PDZ prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00487-12
更新日期:2012-07-01 00:00:00
abstract::A mutant which produces a small-headed ("petite") variant of bacteriophage T4 is described. The mutation (E920g) maps in a new gene (66) between genes 23 and 24. Petite phage particles composed up to 70% of the phage yield. The petite phage was nonviable upon single infection but produced progeny when two or more infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.6.865-876.1970
更新日期:1970-12-01 00:00:00
abstract::Treatment of influenza virus and its purified hemagglutining with glycosidases from Diplococcus pneumoniae, which included beta-galactosidase, beta-N-acetylglucosminidase, and endoglycosidase D, released amino and neutral sugars from the virus and these as well as large oligosaccharides from the purified hemagglutinin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.1.164-171.1978
更新日期:1978-07-01 00:00:00
abstract::Goat and rabbit antisera prepared against a purified Rauscher murine leukemia virus glycoprotein (gp69/71) rapidly neutralized spleen focus-forming virus in Rauscher and Friend virus preparations. Absorption studies revealed that most of the neutralizing activity of goat anti-Rauscher virus gp69/71 serum was directed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.187-189.1974
更新日期:1974-07-01 00:00:00
abstract::Productively infected bovine fibropapillomas were examined for bovine papillomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cytoplasm of basal and lower spinous epithelial cells, coexpressed in the cytoplasm of basal cells with the E5 oncoprotein. E7 was also observed in nucleoli throughout the b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.513-521.2001
更新日期:2001-01-01 00:00:00
abstract:UNLABELLED:To investigate the molecular mechanism(s) by which herpes simplex virus 1 (HSV-1) tegument protein UL51 promotes viral replication, we screened for viral proteins that interact with UL51 in infected cells. Affinity purification of tagged UL51 in HSV-1-infected Vero cells was coupled with immunoblotting of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01258-16
更新日期:2016-09-12 00:00:00
abstract::To date, no vaccine that is safe and effective against herpes simplex virus 2 (HSV-2) disease has been licensed. In this study, we evaluated a DNA prime-formalin-inactivated-HSV-2 (FI-HSV2) boost vaccine approach in the guinea pig model of acute and recurrent HSV-2 genital disease. Five groups of guinea pigs were immu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02521-10
更新日期:2011-04-01 00:00:00
abstract::The E6 oncoprotein of high-risk human papillomavirus type 16 (HPV16) interacts with several nuclear transcription factors and coactivators in addition to cytoplasmic proteins, suggesting that nuclear import of HPV16 E6 plays a role in the cellular transformation process. In this study we have investigated the nuclear ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2330-2337.2003
更新日期:2003-02-01 00:00:00
abstract::Herpesvirus proteins pUL34 and pUL31 form a complex at the inner nuclear membrane (INM) which is necessary for efficient nuclear egress. Pseudorabies virus (PrV) pUL34 is a type II membrane protein of 262 amino acids (aa). The transmembrane region (TM) is predicted to be located between aa 245 and 261, leaving only on...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05484-11
更新日期:2012-02-01 00:00:00
abstract:UNLABELLED:The RNA genome of respiratory syncytial virus (RSV) is constitutively encapsidated by the viral nucleoprotein N, thus forming a helical nucleocapsid. Polymerization of N along the genomic and antigenomic RNAs is concomitant to replication and requires the preservation of an unassembled monomeric nucleoprotei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03666-14
更新日期:2015-04-01 00:00:00
abstract::A malignant glioma cell line was infected with the human T-lymphotropic virus type IIIB isolate of the human immunodeficiency virus. Infection appeared to be latent rather than productive. Through contact with monocytic or lymphoid cells, the virus present in the glioma cells could be transmitted and gave rise to a fu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.1244-1247.1987
更新日期:1987-04-01 00:00:00
abstract::Hepatitis E virus genotype 1 strain Sar55 replicated in subcloned Caco-2 intestinal cells and Huh7 hepatoma cells that had been transfected with in vitro transcribed viral genomes, and hepatitis E virions were released into the culture medium of both cell lines. Virus egress from cells depended on open reading frame 3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00593-10
更新日期:2010-09-01 00:00:00
abstract::Kaposi sarcoma-associated herpesvirus (KSHV) is necessary but not sufficient for primary effusion lymphoma (PEL) development. Alterations in cellular signaling pathways are also a characteristic of PEL. Other B cell lymphomas have acquired an oncogenic mutation in the myeloid differentiation primary response 88 (MYD88...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02123-19
更新日期:2020-05-04 00:00:00
abstract::Viruses with spindle-shaped virions are abundant in diverse environments. Over the years, such viruses have been isolated from a wide range of archaeal hosts. Evolutionary relationships between them remained enigmatic, however. Here, using structural proteins as markers, we define familial ties among these "dark horse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02941-13
更新日期:2014-02-01 00:00:00
abstract::A fibrosarcoma was induced in a hamster by bovine papilloma virus type 2 (BPV2). The content of BPV2 DNA sequences was measured by DNA-DNA and cRNA-DNA hybridizations. The tumor contained approximately 300 BPV2 genome equivalents per cell. Southern blot hybridization indicated that the viral DNA was in free form, the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.945-949.1981
更新日期:1981-09-01 00:00:00
abstract::We analyzed capsomeres of human papillomavirus type 16 (HPV16) consisting of the L1 major structural protein for their ability to trigger a cytotoxic T-cell (CTL) response. To this end, we immunized C57BL/6 mice and used the L1(165-173) peptide for ex vivo restimulation of splenocytes prior to analysis ((51)Cr release...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4635-4645.2003
更新日期:2003-04-01 00:00:00
abstract::Incorporation of the vaccinia virus A36R protein into the outer membrane of intracellular enveloped virions (IEV) is dependent on expression of the A33R protein. Possible interactions of the 200-amino-acid cytoplasmic domain of the A36R protein with itself or with the cytoplasmic domain of the A33R, A34R, B5R, or F12L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4113-4126.2003
更新日期:2003-04-01 00:00:00