Capsid size and deoxyribonucleic acid length: the petite variant of bacteriophage T4.

Abstract:

:A mutant which produces a small-headed ("petite") variant of bacteriophage T4 is described. The mutation (E920g) maps in a new gene (66) between genes 23 and 24. Petite phage particles composed up to 70% of the phage yield. The petite phage was nonviable upon single infection but produced progeny when two or more infected a cell. Its genome was shortened by a random deletion of about 30%, and deoxyribonucleic acid (DNA) extracted from the particles was 0.68 the length of normal T4 DNA. The reduction in DNA length was accompanied by a proportional reduction in head volume. Double mutants between E920g and head-defective mutants in gene 21 produced unusually high frequencies of spherical capsidlike structures (tau-particles).

journal_name

J Virol

journal_title

Journal of virology

authors

Eiserling FA,Geiduschek EP,Epstein RH,Metter EJ

doi

10.1128/JVI.6.6.865-876.1970

subject

Has Abstract

pub_date

1970-12-01 00:00:00

pages

865-76

issue

6

eissn

0022-538X

issn

1098-5514

journal_volume

6

pub_type

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