Abstract:
:A conjugate of Pseudomonas exotoxin and epidermal growth factor (PE-EGF) inhibits proteins synthesis in KB cells, and this inhibition is increased by adenovirus. Protein synthesis inhibition is dependent on the amount of adenovirus and PE-EGF used and the time of incubation of cells with these agents. With 1 microgram of adenovirus and 0.5 micrograms of PE-EGF per ml, protein synthesis is inhibited about 80% in a 60-min experiment. Under these conditions neither adenovirus nor PE-EGF alone has any effect. In the presence of several weak bases or monensin, the enhancement of toxicity was substantially inhibited; half-maximal inhibition was achieved with 40 microM chloroquine, 10 mM ammonium chloride, 5 mM methylamine, 0.1 mM N-hexylamine and 1 microM monensin. At the concentrations employed, none of the inhibitors affected the amount of virus taken up or bound to the cell surface, and chloroquine had no effect on the amount of EGF taken up in 60 min. Chloroquine did not prevent the toxicity of the PE-EGF (5 micrograms/ml) alone. Because these compounds are known to elevate the pH in receptosomes, it seems likely that the acidification of the receptosome either enhances the lysis of the membrane by adenovirus or enhances some other step in the release of PE-EGF.
journal_name
J Viroljournal_title
Journal of virologyauthors
Seth P,Fitzgerald DJ,Willingham MC,Pastan Idoi
10.1128/JVI.51.3.650-655.1984subject
Has Abstractpub_date
1984-09-01 00:00:00pages
650-5issue
3eissn
0022-538Xissn
1098-5514journal_volume
51pub_type
杂志文章abstract::Papillomavirus disease poses a special challenge to people with compromised immune systems. Appropriate models to study infections in these individuals are lacking. We report here the development of a model that will help to address these deficiencies. The MmuPV1 genome was synthesized and used successfully to produce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00777-13
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abstract::Natural transmission of human immunodeficiency virus (HIV) occurs at mucosal surfaces. During acute infection, intestinal and other mucosae are preferential sites of virus replication and rapidly become depleted of CD4(+) T cells. Therefore, mucosal immunity may be critical to control both initial infection and the ma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00894-06
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abstract::The influenza A virus nucleoprotein (NP) is a multifunctional polypeptide which plays a pivotal role in virus replication. To get information on the domains and specific residues involved in the different NP activities, we describe here the preparation and characterization of 20 influenza A virus mutant NPs. The mutat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1186-1194.1999
更新日期:1999-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2723-2732.1998
更新日期:1998-04-01 00:00:00
abstract::The UL11 tegument protein of herpes simplex virus plays a critical role in the secondary envelopment; however, the mechanistic details remain elusive. Here, we report a new function of UL11 in the budding process in which it directs efficient acquisition of glycoprotein E (gE) via a direct interaction. In vitro bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05207-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10714-10718.2003
更新日期:2003-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.460-469.1986
更新日期:1986-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1959-1970.2002
更新日期:2002-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.611-614.1987
更新日期:1987-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1210-1218.1998
更新日期:1998-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.115-120.1975
更新日期:1975-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1011-1012.1984
更新日期:1984-12-01 00:00:00
abstract::RNA-dependent protein kinase PKR is an important regulator of gene expression in interferon (IFN)-treated and virus-infected cells. The 50-kb gene encoding human PKR kinase (pkr) is inducible by IFN. Transfection analyses, using chloramphenicol acetyltransferase (CAT) as the reporter in constructs possessing various 5...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9934-9939.1998
更新日期:1998-12-01 00:00:00
abstract::Current human immunodeficiency virus type 1 (HIV-1) envelope vaccine candidates elicit high antibody binding titers with neutralizing activity against T-cell line-adapted but not primary HIV-1 isolates. Serum antibodies from these human vaccine recipients were also found to be preferentially directed to linear epitope...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4319-4330.1997
更新日期:1997-06-01 00:00:00
abstract::Infection of macaque monkeys with simian immunodeficiency virus (SIV) is probably the best animal model currently available for studying acquired immunodeficiency syndrome. In this report, we describe three infectious molecular clones of SIVmac and one of human immunodeficiency virus type 2 (HIV-2) and their use in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4691-4696.1988
更新日期:1988-12-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) can infect nondividing cells productively because the nuclear import of viral nucleic acids occurs in the absence of cell division. A number of viral factors that are present in HIV-1 preintegration complexes (PICs) have been assigned functions in nuclear import, including a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12087-12096.2002
更新日期:2002-12-01 00:00:00
abstract::The temporal sequence of coronavirus plus-strand and minus-strand RNA synthesis was determined in 17CL1 cells infected with the A59 strain of mouse hepatitis virus (MHV). MHV-induced fusion was prevented by keeping the pH of the medium below pH 6.8. This had no effect on the MHV replication cycle, but gave 5- to 10-fo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.328-334.1986
更新日期:1986-01-01 00:00:00
abstract::Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I . J fragment (the left 7.8% of the viral genome) and in human KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.337-352.1980
更新日期:1980-11-01 00:00:00
abstract::The majority of HIV-1 infections around the world result from non-B clade HIV-1 strains. The CRF01_AE (AE) strain is seen principally in Southeast Asia. AE protease differs by approximately 10% in amino acid sequence from clade B protease and carries several naturally occurring polymorphisms that are associated with d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-10
更新日期:2010-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01140-16
更新日期:2016-10-14 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2615-2623.1994
更新日期:1994-04-01 00:00:00
abstract::To analyze the association of the human immunodeficiency virus type 1 integrase (IN) protein with DNA substrates, we applied a shortwave UV cross-linking method to the in vitro integration system. Three photoadduct bands were detected on sodium dodecyl sulfate-polyacrylamide gels. The appearances of these photoadducts...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5690-5697.1994
更新日期:1994-09-01 00:00:00
abstract::The human genome encodes over 500 microRNAs (miRNAs), small RNAs (19 to 26 nucleotides [nt]) that regulate the expressions of diverse cellular genes. Many cellular processes are altered through a variety of mechanisms by human cytomegalovirus (HCMV) infection. We asked whether HCMV infection leads to changes in the ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00961-08
更新日期:2008-09-01 00:00:00
abstract::The mechanism by which herpes simplex virus 1 (HSV-1) establishes latency in sensory neurons is largely unknown. Recent studies indicate that epigenetic modifications of the chromatin associated with the latent genome may play a key role in the transcriptional control of lytic genes during latency. In this study, we f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00686-09
更新日期:2009-08-01 00:00:00
abstract::A polymorphism in the gene encoding CCR2 is associated with a delay in progression to AIDS in human immunodeficiency virus (HIV)-infected individuals. The polymorphism, CCR2-64I, changes valine 64 of CCR2 to isoleucine. However, it is not clear whether the effect on AIDS progression results from the amino acid change ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2450-2459.1999
更新日期:1999-03-01 00:00:00
abstract::Reovirus attachment protein σ1 engages glycan receptors and junctional adhesion molecule-A (JAM-A) and is thought to undergo a conformational change during the proteolytic disassembly of virions to infectious subvirion particles (ISVPs) that accompanies cell entry. The σ1 protein is also the primary target of neutrali...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01621-16
更新日期:2017-01-31 00:00:00
abstract:UNLABELLED:Despite the nutritional and health benefits of breast milk, breast milk can serve as a vector for mother-to-child HIV transmission. Most HIV-infected infants acquire HIV through breastfeeding. Paradoxically, most infants breastfed by HIV-positive women do not become infected. This is potentially attributed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01702-15
更新日期:2015-11-01 00:00:00
abstract::ts5, a temperature-sensitive mutant of influenza B virus, belongs to one of seven recombination groups. When the mutant infected MDCK cells at the nonpermissive temperature (37.5 degrees C), infectious virus was produced at very low levels compared with the yield at the permissive temperature (32 degrees C) and hemagg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3264-3273.1993
更新日期:1993-06-01 00:00:00
abstract:UNLABELLED:Replication-deficient rabies virus (RABV)-based vaccines induce rapid and potent antibody responses via T cell-independent and T cell-dependent mechanisms. To further investigate early events in vaccine-induced antibody responses against RABV infections, we studied the role of macrophages as mediators of RAB...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03409-14
更新日期:2015-03-01 00:00:00