Persistent activation of STAT3 by latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells.


:Kaposi's sarcoma-associated herpesvirus (KSHV) is the infectious cause of Kaposi's sarcoma, primary effusion lymphoma, and plasmablastic multicentric Castleman's disease. STAT3 has been shown to be important for the maintenance of primary effusion lymphoma cells in culture and is chronically activated in many tumor cell lines. However, little is known about the role of KSHV in the activation of STAT3 or the role of STAT3 in KS tumors. We demonstrate that STAT3 is activated by KSHV infection of endothelial cells, the KS tumor cell type, in a biphasic fashion. Viral binding and entry activate STAT3 in the first 2 h after infection, but this activation dissipates by 4 h postinfection. By 12 h after KSHV infection, concomitant with the expression of latent genes, STAT3 is once again activated, and this activation persists for as long as latent infection is maintained. Activated STAT3 translocates to the nucleus, where it can bind to STAT3-specific DNA elements and can activate STAT3-dependent promoter activity. Conditioned medium from KSHV-infected endothelial cells is able to transiently activate STAT3, indicating the involvement of a secreted factor and that a latency-associated factor in KSHV-infected cells is necessary for sustained activation. KSHV upregulates gp130 receptor expression, and both gp130 and JAK2 are required for the activation of STAT3. However, neither human nor viral interleukin-6 is required for STAT3 activation. Persistent activation of the oncogenic signal transducer, STAT3, by KSHV may play a critical role in the viral pathogenesis of Kaposi's sarcoma, as well as in primary effusion lymphomas.


J Virol


Journal of virology


Punjabi AS,Carroll PA,Chen L,Lagunoff M




Has Abstract


2007-03-01 00:00:00














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    journal_title:Journal of virology

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    authors: Kessel M,Varon M

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    authors: Zhou S,Standring DN

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    authors: Zielecki F,Semmler I,Kalthoff D,Voss D,Mauel S,Gruber AD,Beer M,Wolff T

    更新日期:2010-10-01 00:00:00

  • Induction of human cell DNA synthesis by herpes simplex virus type 2.

    abstract::The effect of herpes simplex virus type 2 (HSV-2) infection on the synthesis of DNA in human embryonic fibroblast cells was determined at temperatures permissive (37 C) and nonpermissive (42 C) for virus multiplication. During incubation of HSV-2 infected cultures at 42 C for 2 to 4 days or after shift-down from 42 to...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Melvin P,Kucera LS

    更新日期:1975-03-01 00:00:00

  • E5 proteins of human papillomavirus types 11 and 16 transactivate the c-fos promoter through the NF1 binding element.

    abstract::Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or H...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Chen SL,Lin YK,Li LY,Tsao YP,Lo HY,Wang WB,Tsai TC

    更新日期:1996-12-01 00:00:00

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    abstract::Treatment of human immunodeficiency virus type 1 (HIV-1)-infected CEM cell cultures with escalating concentrations of the quinoxaline S-2720 resulted in an ordered appearance of single and multiple mutant virus strains that gradually became resistant to the quinoxaline and other nonnucleoside reverse transcriptase (RT...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pelemans H,Esnouf R,Dunkler A,Parniak MA,Vandamme AM,Karlsson A,De Clercq E,Kleim JP,Balzarini J

    更新日期:1997-11-01 00:00:00

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Iwamoto T,Okinaka Y,Mise K,Mori K,Arimoto M,Okuno T,Nakai T

    更新日期:2004-02-01 00:00:00

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    abstract::BALB/c mice treated with pristane and Abelson virus have been used as an animal model system for the rapid induction of plasmacytomas. Myelomonocytic tumors with helper Moloney murine leukemia virus clonally inserted into the c-myb locus were observed in about 10% of pristane-primed BALB/c mice infected with Abelson v...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Shen-Ong GL,Wolff L

    更新日期:1987-12-01 00:00:00

  • Activation of STAT transcription factors by herpesvirus Saimiri Tip-484 requires p56lck.

    abstract::Signal transducers and activators of transcription (STATs) relay signals from activated cell surface receptors directly to the nucleus. Previously, a protein required for T-cell transformation by the DNA tumor virus herpesvirus saimiri (HVS) and designated tyrosine kinase interacting protein (Tip-484) was shown to int...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lund TC,Garcia R,Medveczky MM,Jove R,Medveczky PG

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    abstract::Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Komoto S,Kugita M,Sasaki J,Taniguchi K

    更新日期:2008-07-01 00:00:00

  • RNA 3' readthrough of oncoretrovirus and lentivirus: implications for vector safety and efficacy.

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    authors: Zaiss AK,Son S,Chang LJ

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    abstract::We recently demonstrated that members of the c-ets proto-oncogene family, Ets1 and Ets2, are sequence-specific transcriptional activators of the human T-lymphotropic virus type I (HTLV-I) long terminal repeat (LTR). We now report that the HTLV-I LTR contains two distinct Ets1-responsive regions, ERR-1 and ERR-2. Expre...

    journal_title:Journal of virology

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    authors: Gitlin SD,Bosselut R,Gégonne A,Ghysdael J,Brady JN

    更新日期:1991-10-01 00:00:00

  • A novel mutation in murine hepatitis virus nsp5, the viral 3C-like proteinase, causes temperature-sensitive defects in viral growth and protein processing.

    abstract::Sequencing and reversion analysis of murine hepatitis virus (MHV) temperature-sensitive (ts) viruses has identified putative ts mutations in the replicase nonstructural proteins (nsp's) of these coronaviruses. In this study, reverse transcriptase PCR sequencing of the RNA genome of an isolate of the MHV ts virus Alb t...

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    authors: Sparks JS,Donaldson EF,Lu X,Baric RS,Denison MR

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    authors: O'Carroll IP,Thappeta Y,Fan L,Ramirez-Valdez EA,Smith S,Wang YX,Rein A

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  • Apoptosis induced by infection of primary brain cultures with diverse human immunodeficiency virus type 1 isolates: evidence for a role of the envelope.

    abstract::Apoptosis of neurons and astrocytes is induced by human immunodeficiency type 1 (HIV-1) infection in vitro and has been demonstrated in brain tissue from patients with AIDS. We analyzed a panel of diverse HIV-1 primary isolates for the ability to replicate and induce neuronal and astrocyte apoptosis in primary human b...

    journal_title:Journal of virology

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    authors: Ohagen A,Ghosh S,He J,Huang K,Chen Y,Yuan M,Osathanondh R,Gartner S,Shi B,Shaw G,Gabuzda D

    更新日期:1999-02-01 00:00:00

  • Identification and Characterization of a Human Coronavirus 229E Nonstructural Protein 8-Associated RNA 3'-Terminal Adenylyltransferase Activity.

    abstract::Coronavirus nonstructural protein 8 (nsp8) has been suggested to have diverse activities, including noncanonical template-dependent polymerase activities. Here, we characterized a recombinant form of the human coronavirus 229E (HCoV-229E) nsp8 and found that the protein has metal ion-dependent RNA 3'-terminal adenylyl...

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    authors: Tvarogová J,Madhugiri R,Bylapudi G,Ferguson LJ,Karl N,Ziebuhr J

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    journal_title:Journal of virology

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    authors: Rhim H,Rice AP

    更新日期:1993-02-01 00:00:00

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    abstract::Some of the properties of a new human papovavirus, BK, have been examined. Host range studies of BK virus (BKV) showed human cells to be more sensitive to infection than monkey cells; human fetal brain cells appear to be highly sensitive to BKV, with the production of extensive cytopathology characterized by cytoplasm...

    journal_title:Journal of virology

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    authors: Takemoto KK,Mullarkey MF

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Tellinghuisen TL,Foss KL,Treadaway JC,Rice CM

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  • Monoclonal antibodies against influenza virus PB2 and NP polypeptides interfere with the initiation step of viral mRNA synthesis in vitro.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bárcena J,Ochoa M,de la Luna S,Melero JA,Nieto A,Ortín J,Portela A

    更新日期:1994-11-01 00:00:00

  • Experimental coinfection of rhesus macaques with rhesus cytomegalovirus and simian immunodeficiency virus: pathogenesis.

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    authors: Sequar G,Britt WJ,Lakeman FD,Lockridge KM,Tarara RP,Canfield DR,Zhou SS,Gardner MB,Barry PA

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  • A specific region of 37 amino acid residues in the SPRY (B30.2) domain of African green monkey TRIM5alpha determines species-specific restriction of simian immunodeficiency virus SIVmac infection.

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    journal_title:Journal of virology

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Garnier L,Ratner L,Rovinski B,Cao SX,Wills JW

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Highlander SL,Dorney DJ,Gage PJ,Holland TC,Cai W,Person S,Levine M,Glorioso JC

    更新日期:1989-02-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: de Vries AA,Raamsman MJ,van Dijk HA,Horzinek MC,Rottier PJ

    更新日期:1995-06-01 00:00:00

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    abstract::Myxomatosis is a recurrent problem on rabbit farms throughout Europe despite the success of vaccines. To identify gene variations of field and vaccine strains that may be responsible for changes in virulence, immunomodulation, and immunoprotection, the genomes of 6 myxoma virus (MYXV) strains were sequenced: German fi...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Braun C,Thürmer A,Daniel R,Schultz AK,Bulla I,Schirrmeier H,Mayer D,Neubert A,Czerny CP

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Foghsgaard L,Jäättelä M

    更新日期:1997-10-01 00:00:00