Abstract:
:The 55-kDa E1B protein of adenovirus, which binds to and inactivates the tumor suppressor protein p53, is not expressed in the adenoviral mutant termed ONYX-015 (i.e., dl1520). It was reported that the mutant virus due to a deletion in E1B is able to replicate only in cells deficient for wild-type p53. Accordingly, dl1520 is currently being evaluated as a potential tool in the therapy of p53 deficient cancers. In contrast, we report here that dl1520 replicates independently of the p53 status in various tumor cell lines (U87, RKO, A549, H1299, and U373). In addition, the inhibition of p53-mediated transcriptional activation in wild-type p53 containing U2OS cells, by overexpression of a transdominant negative p53 mutant, did not render the cells permissive for dl1520 replication. Finally, we show that, depending on the multiplicity of infection, the deleted virus is able to replicate in and to kill primary human cells. Thus, the molecular basis for the growth differences of dl1520 within different cell types remains to be determined.
journal_name
J Viroljournal_title
Journal of virologyauthors
Rothmann T,Hengstermann A,Whitaker NJ,Scheffner M,zur Hausen Hdoi
10.1128/JVI.72.12.9470-9478.1998subject
Has Abstractpub_date
1998-12-01 00:00:00pages
9470-8issue
12eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mi...
journal_title:Journal of virology
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doi:10.1128/JVI.13.1.42-45.1974
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pub_type: 杂志文章
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更新日期:1986-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.67.10.6322-6326.1993
更新日期:1993-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.3.1074-1082.1980
更新日期:1980-03-01 00:00:00
abstract::Marek's disease virus (MDV) is an alphaherpesvirus for which infection is strictly cell associated in permissive cell culture systems. In contrast to most other alphaherpesviruses, no comprehensive ultrastructural study has been published to date describing the different stages of MDV morphogenesis. To circumvent prob...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01177-07
更新日期:2007-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.3.244-251.1969
更新日期:1969-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.73.1.1-10.1999
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.3.847-853.1982
更新日期:1982-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1739-1745.1993
更新日期:1993-03-01 00:00:00
abstract::Polyomaviruses are small nonenveloped particles with a circular double-stranded genome, approximately 5 kbp in size. The mammalian polyomaviruses mainly cause persistent subclinical infections in their natural nonimmunocompromised hosts. In contrast, the polyomaviruses of birds--avian polyomavirus (APV) and goose hemo...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.4.430-436.1971
更新日期:1971-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01510-17
更新日期:2018-03-28 00:00:00