Abstract:
:Small amounts of infectious simian virus 40 (SV40) were recovered from parental cultures of SV40-transformed human embryonic lung (WI38 Va13A) cells, from 12 primary clones, from 17 secondary clones, and from 18 tertiary clones. The cloning experiments demonstrated that the capacity for spontaneous virus production is a hereditary property of WI38 Va13A cells. Infectious virus was not recovered from every clone at every passage. Repeated trials at different passage levels were necessary to detect virus production. Approximately one in 10(5) to 10(6) of the cells of the clonal lines initiated plaque formation when plated on the CV-1 line of African green monkey kidney cells. No increase in infectious center formation was observed after the clonal lines were treated with bromodeoxyuridine, iododeoxyuridine, or mitomycin C or after heterokaryon formation of treated cells with CV-1 cells. The clonal lines of WI38 Va13A cells were susceptible to superinfection by SV40 deoxyribonucleic acid (DNA). To determine whether only those cells which spontaneously produced virus supported the replication of superinfecting SV40 DNA, cultures were infected with DNA from a plaque morphology mutant and a temperature-sensitive mutant of SV40. After infection by SV40 DNA, approximately 100 to 4,400 times more transformed cells formed infectious centers than were spontaneously producing virus. To determine whether the resident SV40 genome or the superinfecting SV40 genome was replicating, infectious centers produced by SV40 DNA-infected WI38 Va13A cells on CV-1 monolayers were picked and the progeny virus was analyzed. Only the superinfecting SV40 was recovered from the infectious centers, indicating that in the majority of superinfected cells the resident SV40 was not induced to replicate.
journal_name
J Viroljournal_title
Journal of virologyauthors
Dubbs DR,Kit Sdoi
10.1128/JVI.8.4.430-436.1971subject
Has Abstractpub_date
1971-10-01 00:00:00pages
430-6issue
4eissn
0022-538Xissn
1098-5514journal_volume
8pub_type
杂志文章abstract::Coronaviruses are the largest RNA viruses, and their genomes encode replication machinery capable of efficient replication of both positive- and negative-strand viral RNAs as well as enzymes capable of processing large viral polyproteins into putative replication intermediates and mature proteins. A model described re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00049-07
更新日期:2007-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.34-42.1979
更新日期:1979-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.65.10.5535-5538.1991
更新日期:1991-10-01 00:00:00
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pub_type: 杂志文章
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abstract::JC polyomavirus (JCV) infection is highly prevalent and usually kept in a persistent state without clinical signs and symptoms. It is only during immunocompromise and especially impaired CD4(+) T cell function in the brain, as seen in AIDS patients or natalizumab-treated multiple sclerosis patients, that JCV may cause...
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pub_type: 杂志文章
doi:10.1128/JVI.79.16.10171-10179.2005
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abstract::The human immunodeficiency virus (HIV) Tat protein has a critical role in viral transcription, but this study focuses on its additional role as an extracellular effector of lymphocyte cell death. It is well known that Tat induces tumor necrosis factor-related apoptosis-induced ligand (TRAIL) in peripheral blood mononu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.6700-6708.2003
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6768-6777.1993
更新日期:1993-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.626-628.1983
更新日期:1983-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.1.212-215.1986
更新日期:1986-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.12075-12081.2004
更新日期:2004-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01679-08
更新日期:2009-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.78.2.995-998.2004
更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00158-12
更新日期:2012-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6761-6764.1991
更新日期:1991-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.236-244.1998
更新日期:1998-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5189-5196.2001
更新日期:2001-06-01 00:00:00
abstract::Epstein-Barr virus (EBV)-induced B-cell growth transformation, a central feature of the virus' strategy for colonizing the human B-cell system, requires full virus latent gene expression and is initiated by transcription from the viral promoter Wp. Interestingly, when EBV accesses other cell types, this growth-transfo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10458-10467.2000
更新日期:2000-11-01 00:00:00
abstract:UNLABELLED:Hepatitis B, which caused by hepatitis B virus (HBV) infection, remains a major health threat worldwide. Hepatic injury and regeneration from chronic inflammation are the main driving factors of liver fibrosis and cirrhosis in chronic hepatitis B. Proinflammatory tumor necrosis factor alpha (TNF-α) has been ...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.591-593.1984
更新日期:1984-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.66.7.4191-4200.1992
更新日期:1992-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3837-3843.1989
更新日期:1989-09-01 00:00:00
abstract:UNLABELLED:The HIV-1 envelope protein (Env) is heavily glycosylated, with approximately 50% of the Env molecular mass being contributed by N-glycans. HIV-1 Env N-glycans shield the protein backbone and have been shown to play key roles in determining Env structure, surface exposure, and, consequently, antigenicity, inf...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00764-17
更新日期:2017-08-10 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00356-15
更新日期:2015-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5431-5437.1999
更新日期:1999-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7440-7449.1998
更新日期:1998-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3104-3107.1990
更新日期:1990-06-01 00:00:00
abstract::A number of herpes simplex virus (HSV) glycoproteins are found in oligomeric states: glycoprotein E (gE)-gI and gH-gL form heterodimers, and both gB and gC have been detected as homodimers. We have further explored the organization of glycoproteins in the virion envelope by using both purified virions to quantitate gl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6067-6070.1996
更新日期:1996-09-01 00:00:00