Abstract:
:The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of the putative fusion peptide (PEDV-SFCS). PEDV-SFCS exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independently of trypsin, though to low titer.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li W,Wicht O,van Kuppeveld FJ,He Q,Rottier PJ,Bosch BJdoi
10.1128/JVI.00356-15subject
Has Abstractpub_date
2015-08-01 00:00:00pages
8077-81issue
15eissn
0022-538Xissn
1098-5514pii
JVI.00356-15journal_volume
89pub_type
杂志文章abstract::The large-particle fraction from the cytoplasm of chick embryo fibroblasts infected with Semliki Forest virus was found to catalyze the incorporation of the 5'-triphosphates of guanosine, adenine, cytidine, and uridine into an acid-insoluble alkali-labile product. The conditions affecting the preparation and assay of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.1.97-109.1967
更新日期:1967-02-01 00:00:00
abstract::We previously reported that the adenovirus type 5 E3 14.5-kilodalton protein (14.5K) forms a complex with E3 10.4K and that both proteins are required to down-regulate the epidermal growth factor receptor in adenovirus-infected human cells. Both proteins are also required to prevent cytolysis by tumor necrosis factor ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.3.1665-1673.1992
更新日期:1992-03-01 00:00:00
abstract::Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.42-45.1974
更新日期:1974-01-01 00:00:00
abstract::The major immediate-early (MIE) transactivator proteins of cytomegaloviruses (CMV) play a pivotal role in the initiation of virus-host cell interactions. Therefore, cis- and trans-acting factors influencing the expression of these proteins through their upstream promoter-enhancer regions are important determinants of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.516-529.1993
更新日期:1993-01-01 00:00:00
abstract::Fusion of the membrane of the Moloney murine leukemia virus (Mo-MLV) Env protein is facilitated by cleavage of the R peptide from the cytoplasmic tail of its TM subunit, but the mechanism for this effect has remained obscure. The fusion is also controlled by the isomerization of the intersubunit disulfide of the Env S...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02039-07
更新日期:2008-03-01 00:00:00
abstract::Mononuclear phagocytes (MP) and T lymphocytes play a pivotal role in the host immune response to human immunodeficiency virus type 1 (HIV-1) infection. Regulation of such immune responses can be mediated, in part, through the interaction of the T-lymphocyte-expressed molecule CD40 ligand (CD40L) with its receptor on M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4308-4320.2001
更新日期:2001-05-01 00:00:00
abstract::GP64, the major envelope glycoprotein of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) budded virion, is important for host cell receptor binding and mediates low-pH-triggered membrane fusion during entry by endocytosis. In the current study, we examined the functional role of the AcMNPV GP64 tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02104-07
更新日期:2008-04-01 00:00:00
abstract::The role of the 5' nontranslated region in the replication of hepatitis A virus (HAV) was studied by analyzing the translation and replication of chimeric RNAs containing the encephalomyocarditis virus (EMCV) internal ribosome entry segment (IRES) and various lengths (237, 151, or 98 nucleotides [nt]) of the 5'-termin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.2861-2868.1996
更新日期:1996-05-01 00:00:00
abstract:UNLABELLED:Rotaviruses (RVs) are 11-segmented, double-stranded RNA viruses that cause severe gastroenteritis in children. In addition to an error-prone genome replication mechanism, RVs can increase their genetic diversity by reassorting genes during host coinfection. Such exchanges allow RVs to acquire advantageous ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01562-14
更新日期:2014-09-01 00:00:00
abstract::The polypeptides of three paramyxoviruses (simian virus 5, Newcastle disease virus, and Sendai virus) were separated by polyacrylamide gel electrophoresis. Glycoproteins were identified by the use of radioactive glucosamine as a carbohydrate precursor. The protein patterns reveal similarities among the three viruses. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.1.47-52.1971
更新日期:1971-01-01 00:00:00
abstract::A number of naturally occurring hepatitis B virus mutants that cannot synthesize the virus precore protein have been identified. Such mutants have been associated with more severe forms of hepatitis, including fulminant hepatitis. The most common mutation observed is a substitution of G to A in the distal precore gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5682-5684.1992
更新日期:1992-09-01 00:00:00
abstract::The host cell receptor for Moloney murine leukemia virus was solubilized from murine L-cell membranes and characterized. In initial studies designed to identify a receptor-rich cell line, different mouse cells were screened for binding to Moloney gp70, the viral envelope glycoprotein which determines host cell-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.900-908.1986
更新日期:1986-06-01 00:00:00
abstract::Adeno-associated viruses (AAVs) are small single-stranded DNA viruses that can package and deliver nongenomic DNA for therapeutic gene delivery. AAV8, a liver-tropic vector, has shown great promise for the treatment of hemophilia A and B. However, as with other AAV vectors, host anti-capsid immune responses are a dete...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00218-12
更新日期:2012-08-01 00:00:00
abstract::Massive infection of memory CD4 T cells is a hallmark of early simian immunodeficiency virus (SIV) infection, with viral infection peaking at day 10 postinfection (p.i.), when a majority of memory CD4 T cells in mucosal and peripheral tissues are infected. It is not clear if mononuclear cells from the monocyte and mac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06372-11
更新日期:2012-01-01 00:00:00
abstract::Influenza virus enters its host cell by receptor-mediated endocytosis followed by acid-activated membrane fusion in endosomes. The viral ribonucleoprotein particles (vRNPs) delivered into the cytosol then dissociate from the matrix protein, M1, and from each other, after which they are individually imported into the n...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8391-8401.1996
更新日期:1996-12-01 00:00:00
abstract::The tempo and intensity of retroviral neuropathogenesis are dependent on the capacity of the virus to invade the central nervous system. For murine leukemia viruses, an important determinant of neuroinvasiveness is the virus-encoded protein glycosylated Gag, the function of which in the virus life cycle is not known. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5619-5625.1998
更新日期:1998-07-01 00:00:00
abstract:UNLABELLED:While numerous viral microRNAs (miRNAs) expressed by DNA viruses, especially herpesvirus family members, have been reported, there have been very few reports of miRNAs derived from RNA viruses. Here we describe three miRNAs expressed by bovine foamy virus (BFV), a member of the spumavirus subfamily of retrov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03587-13
更新日期:2014-05-01 00:00:00
abstract::The mechanism whereby picornaviruses inhibit host protein synthesis while their own synthetic processes proceed unabated has remained elusive. One of our approaches to this problem was to study the ability of cell-free extracts derived from uninfected and mengovirus-infected Ehrlich ascites tumor cells to translate vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.182-194.1976
更新日期:1976-04-01 00:00:00
abstract::Murine gammaherpesvirus 68 (gammaHV68, or MHV-68) is a genetically tractable, small animal model for the analysis of gammaherpesvirus pathogenesis. The gammaHV68 genome is colinear with the genomes of other sequence gammaherpesviruses, containing large blocks of conserved genes interspersed by a number of putative gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1973-1984.2000
更新日期:2000-02-01 00:00:00
abstract::Astrovirus is one of the major causes of infant and childhood diarrhea worldwide. Our understanding of astrovirus pathogenesis trails behind our knowledge of its molecular and epidemiologic properties. Using a recently developed small-animal model, we investigated the mechanisms by which astrovirus induces diarrhea an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1564-1574.2004
更新日期:2004-02-01 00:00:00
abstract::The 72-kDa immediate early 1 (IE1) protein encoded by human cytomegalovirus (hCMV) is a nuclearly localized promiscuous regulator of viral and cellular transcription. IE1 has long been known to associate with host mitotic chromatin, yet the mechanisms underlying this interaction have not been specified. In this study,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02606-13
更新日期:2014-01-01 00:00:00
abstract::Infection of domestic rabbits with cottontail rabbit papillomavirus (CRPV) causes local papillomas which progress to carcinomas in more than 80% of cases. This animal model system therefore allows the identification of molecular mechanisms required for the induction and progression of epithelial tumors. The viral E2 p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11209-11215.2002
更新日期:2002-11-01 00:00:00
abstract::Infection of microglia and other cells of the macrophage/monocyte lineage in the central nervous system (CNS) by human immunodeficiency virus type I (HIV-1) underlies the development of giant cell encephalitis (GCE). It is currently unknown whether GCE depends on the emergence of virus populations specifically adapted...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11686-11699.2001
更新日期:2001-12-01 00:00:00
abstract::The human papillomavirus 1 (HPV-1) virion is composed of two virally encoded proteins: a 57,000-molecular-weight polypeptide (57K polypeptide), which is the product of the L1 open reading frame (ORF), and a 78K polypeptide, which is derived from the L2 ORF. The 57K (L1) product, which represents the major structural c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2793-2799.1987
更新日期:1987-09-01 00:00:00
abstract::A radioimmunoassay (RIA) has been used to measure the quantity of protein P27 (mol wt 27,000; group specific-1 [gs-1]) of avian leukoviruses in different types of chicken embryos and tissues of adult chickens. The RIA used was 200- to 300-fold more sensitive than the complement fixation test and was able to detect as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.340-346.1974
更新日期:1974-02-01 00:00:00
abstract::The interaction of simian virus 40 (SV40) T antigen (T-ag) with the viral origin has served as a model for studies of site-specific recognition of a eukaryotic replication origin and the mechanism of DNA unwinding. These studies have revealed that a motif termed the "beta-hairpin" is necessary for assembly of T-ag on ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02451-06
更新日期:2007-05-01 00:00:00
abstract::After the contagion measles virus (MV) crosses the respiratory epithelium within myeloid cells that express the primary receptor signaling lymphocytic activation molecule (SLAM), it replicates briskly in SLAM-expressing cells in lymphatic organs. Later, the infection spreads to epithelia expressing nectin-4, an adhere...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03037-12
更新日期:2013-03-01 00:00:00
abstract::Bovine herpesvirus 1 (BoHV-1) is an alphaherpesvirus that causes disease in cattle populations worldwide. Sphingomyelin (SM) is the most abundant sphingolipid in the mammalian cell membrane, where it preferentially associates with cholesterol to form lipid raft domains. SM is a substrate for the lysosome-resident enzy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01547-18
更新日期:2019-02-19 00:00:00
abstract::T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.1155-1159.1976
更新日期:1976-06-01 00:00:00
abstract::The murine polyomavirus (Py) enters mouse fibroblasts and kidney epithelial cells via an endocytic pathway that is caveola-independent (as well as clathrin-independent). In contrast, uptake of simian virus 40 into the same cells is dependent on caveola. Following the initial uptake of Py, both microtubules and microfi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2615-2622.2003
更新日期:2003-02-01 00:00:00