Abstract:
:Influenza virus enters its host cell by receptor-mediated endocytosis followed by acid-activated membrane fusion in endosomes. The viral ribonucleoprotein particles (vRNPs) delivered into the cytosol then dissociate from the matrix protein, M1, and from each other, after which they are individually imported into the nucleus via the nuclear pores. For some time, it has been believed that the low pH in endosomes may, in some way, trigger the capsid disassembly events necessary for nuclear transport. This report provides direct evidence that the association of M1 with vRNPs is sensitive to mildly acidic pH within the infected cell. Recombinant M1, expressed in cultured cells, was found to associate with vRNPs and inhibit their nuclear import. Brief acidification of the cytosolic compartment eliminated the interfering activity and allowed the incoming vRNPs to enter the nucleus. Newly assembled progeny M1-vRNP complexes in the cytosol of infected cells were also dissociated by brief acidification. Acidic pH was thus found to serve as a switch that allowed M1 to carry out its multiple functions in the uncoating, nuclear transport, and assembly of vRNPs.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bui M,Whittaker G,Helenius Adoi
10.1128/JVI.70.12.8391-8401.1996subject
Has Abstractpub_date
1996-12-01 00:00:00pages
8391-401issue
12eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
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pub_type: 杂志文章
doi:10.1128/JVI.70.10.7318-7321.1996
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pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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pub_type: 杂志文章
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