Abstract:
:We have investigated the susceptibility of cord blood monocyte-derived macrophages to human immunodeficiency virus type 1 (HIV-1) infection in vitro. Cord blood monocytes were maintained in vitro for 10 to 15 days and then infected with HIV-1. Syncytia were observed 14 days after infection by light microscopy. Viral proteins were detected by immunofluorescence assay. Electron microscopic examination demonstrated typical lentivirus particles within cytoplasmic vacuoles. The supernatants from the HIV-1-infected cultures also contained significant reverse transcriptase activity and p24 antigen. Like adult monocyte/macrophages, cord-derived monocyte/macrophages expressed the CD4 receptor molecule. Pretreatment with blocking antibody prior to infection with HIV-1 Bal significantly reduced or blocked infection of cord monocyte/macrophages. When cord and adult monocyte/macrophages were infected with HIV-1 Bal or Ada-M and directly compared, higher reverse transcriptase activities and p24 antigen expression were obtained with cord monocyte/macrophages. However, no significant difference was found between adult and cord monocyte/macrophages infected with HIV-1 IIIB. These observations suggest that cord monocyte-derived macrophages may be important in the pathogenesis of pediatric AIDS and that the increased susceptibility of cord monocyte/macrophages to HIV-1 infection in vitro may be relevant to the enhanced susceptibility of neonates to HIV-1 diseases in vivo.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ho WZ,Lioy J,Song L,Cutilli JR,Polin RA,Douglas SDdoi
10.1128/JVI.66.1.573-579.1992subject
Has Abstractpub_date
1992-01-01 00:00:00pages
573-9issue
1eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::Genetic and biochemical studies have suggested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses capsids, but its arrangement remained unknown. Here, we report the first visualization of a unique vertex in the Kaposi's sarcoma-associated herpesvirus (KSHV) capsid by cryoelec...
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pub_type: 杂志文章
doi:10.1128/JVI.65.2.672-676.1991
更新日期:1991-02-01 00:00:00
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更新日期:1980-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3624-3630.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4829-4831.1997
更新日期:1997-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7565-7567.1992
更新日期:1992-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.61.2.405-410.1987
更新日期:1987-02-01 00:00:00