Abstract:
:The Epstein-Barr virus (EBV) immediate-early protein BZLF1 mediates the switch between the latent and lytic forms of EBV infection and has been previously shown to induce a G(1)/S block in cell cycle progression in some cell types. To examine the effect of BZLF1 on cellular gene expression, we performed microarray analysis on telomerase-immortalized human keratinocytes that were mock infected or infected with a control adenovirus vector (AdLacZ) or a vector expressing the EBV BZLF1 protein (AdBZLF1). Cellular genes activated by BZLF1 expression included E2F-1, cyclin E, Cdc25A, and a number of other genes involved in cell cycle progression. Immunoblot analysis confirmed that BZLF1 induced expression of E2F-1, cyclin E, Cdc25A, and stem loop binding protein (a protein known to be primarily expressed during S phase) in telomerase-immortalized keratinocytes. Similarly, BZLF1 increased expression of E2F-1, cyclin E, and stem loop binding protein (SLBP) in primary tonsil keratinocytes. In contrast, BZLF1 did not induce E2F-1 expression in normal human fibroblasts. Cell cycle analysis revealed that while BZLF1 dramatically blocked G(1)/S progression in normal human fibroblasts, it did not significantly affect cell cycle progression in primary human tonsil keratinocytes. Furthermore, in EBV-infected gastric carcinoma cells, the BZLF1-positive cells had an increased number of cells in S phase compared to the BZLF1-negative cells. Thus, in certain cell types (but not others), BZLF1 enhances expression of cellular proteins associated with cell cycle progression, which suggests that an S-phase-like environment may be advantageous for efficient lytic EBV replication in some cell types.
journal_name
J Viroljournal_title
Journal of virologyauthors
Mauser A,Holley-Guthrie E,Zanation A,Yarborough W,Kaufmann W,Klingelhutz A,Seaman WT,Kenney Sdoi
10.1128/jvi.76.24.12543-12552.2002subject
Has Abstractpub_date
2002-12-01 00:00:00pages
12543-52issue
24eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
杂志文章abstract::Herpes simplex virus type 1 (HSV-1) infection causes the active degradation of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), and this process is reliant on the expression of the HSV-1 immediate-early protein Vmw110. In this study we investigated in more detail the mechanism by which the degradation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.650-657.1999
更新日期:1999-01-01 00:00:00
abstract::Paramyxoviruses such as human parainfluenza viruses that bear inserts encoding protective antigens of heterologous viruses can induce an effective immunity against the heterologous viruses in experimental animals. However, vectors based on common human pathogens would be expected to be restricted in replication in the...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
abstract::Cottontail rabbit papillomavirus (CRPV)-induced papillomas progress at a high frequency to carcinomas and thus can serve as a model for high-cancer-risk human papillomavirus infection. Previously, we have shown that antibodies to nonstructural and structural proteins are detected in only a fraction of papilloma-bearin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.4043-4048.1994
更新日期:1994-06-01 00:00:00
abstract::Six monoclonal antibodies were isolated that exhibited specificity for a furin cleavage site deletion mutant (V3526) of Venezuelan equine encephalitis virus (VEEV). These antibodies comprise a single competition group and bound the E3 glycoprotein of VEEV subtype I viruses but failed to bind the E3 glycoprotein of oth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01345-10
更新日期:2010-12-01 00:00:00
abstract::Retroviral genomes are dimeric, comprised of two sense-strand RNAs linked at their 5' ends by noncovalent base pairing and tertiary interactions. Viral maturation involves large-scale morphological changes in viral proteins and in genomic RNA dimer structures to yield infectious virions. Structural studies have largel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01602-09
更新日期:2010-01-01 00:00:00
abstract::Recombinant protein subunit AIDS vaccines have been based predominantly on the virus envelope protein. Such vaccines elicit neutralizing antibody responses that can provide type-specific sterilizing immunity, but in most cases do not confer protection against divergent viruses. In this report we demonstrate that a mul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1049-1058.2003
更新日期:2003-01-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) tegument is the least understood component of the virion, and the mechanism of tegument assembly and incorporation into virions during viral egress has not yet been elucidated. In the present study, the addition of tegument proteins (VP13/14, VP16, VP22, and US9) and envelope gl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9934-9951.2002
更新日期:2002-10-01 00:00:00
abstract:UNLABELLED:Bluetongue is one of the major infectious diseases of ruminants and is caused by bluetongue virus (BTV), an arbovirus existing in nature in at least 26 distinct serotypes. Here, we describe the development of a vaccine platform for BTV. The advent of synthetic biology approaches and the development of revers...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02183-14
更新日期:2014-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2268-2273.1992
更新日期:1992-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.20.11125-11138.2003
更新日期:2003-10-01 00:00:00
abstract::The nucleic acids of influenza A, B, and C viruses were compared. Susceptibility to nucleases demonstrates that influenza C virus, just as influenza A and B viruses, possesses single-stranded RNA as its genome. The base compositions of the RNAs of influenza A, B, and influenza C virus are almost identical and comparat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.738-744.1976
更新日期:1976-05-01 00:00:00
abstract::In contrast to humans, several primate species are believed to have harbored simian immunodeficiency viruses (SIVs) since ancient times. In particular, the geographically dispersed species of African green monkeys (AGMs) are all infected with highly diversified SIVagm viruses at high prevalences (greater than 50% of s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8449-8460.2001
更新日期:2001-09-01 00:00:00
abstract::Alternative splicing has been recognized as a major mechanism for creating proteomic diversity from a limited number of genes. However, not all determinants regulating this process have been characterized. Using subviral human immunodeficiency virus (HIV) env constructs we observed an enhanced splicing of the RNA when...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02105-06
更新日期:2007-04-01 00:00:00
abstract::The adenovirus type 2 L1 region, which is located at 30.7 to 39.2 map units on the viral genome, is transcribed from the major late promoter during both early and late stages of virus replication, and a 52,000-Mr (52K) protein-55K protein doublet has been translated in vitro on L1-specific RNA. To investigate the bios...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.839-847.1986
更新日期:1986-03-01 00:00:00
abstract::Cells nonproductively transformed by a variant of the Snyder-Theilen strain of feline sarcoma virus (FeSV) expressed an 85,000-dalton polyprotein (P85) with associated tyrosine-specific protein kinase activity. We identified within this polyprotein a single tyrosine acceptor site for its enzyme activity. This acceptor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.3.886-894.1981
更新日期:1981-06-01 00:00:00
abstract::Identification of cellular receptors used by coronavirus (CoV) entry into the host cells is critical to an understanding of pathogenesis and to development of intervention strategies. The fourth CoV genus, Deltacoronavirus, evolutionarily related to the Gammacoronavirus, has just been defined recently. In the current ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00318-18
更新日期:2018-05-29 00:00:00
abstract::Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is known to take an endosomal pathway for cell entry; however, it is thought to enter directly from the cell surface when a receptor-bound virion spike (S) protein is affected by trypsin, which induces cleavage of the S protein and activates its fusion po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01412-08
更新日期:2008-12-01 00:00:00
abstract::Gene transfer to differentiated airway epithelia with existing viral vectors is very inefficient when they are applied to the apical surface. This largely reflects the polarized distribution of receptors on the basolateral surface. To identify new receptor-ligand interactions that might be used to redirect vectors to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9234-9239.2000
更新日期:2000-10-01 00:00:00
abstract::The cytotoxic T-lymphocyte (CTL) response to lymphocytic choriomeningitis virus infection determines the outcome of infection. Here we show that this response in BALB/c mice (H-2d), when analyzed both at the primary CTL level and using CTL clones, is predominantly monospecific. The vast majority of CTL have a common s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.10.4303-4310.1989
更新日期:1989-10-01 00:00:00
abstract::The synthesis of viral RNA by wild-type vesicular stomatitis virus (L(1)VSV) and a small, plaque-size mutant (S(2)VSV) was studied in vitro and in chicken embryo (CE) and mouse L-cell cultures. Virus-specific RNA synthesized in CE or L cells infected with either L(1) or S(2)VSV at low multiplicity was of the same size...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.2.253-264.1973
更新日期:1973-08-01 00:00:00
abstract::The three primary capsid proteins (A, B, and C) of adeno-associated viruses have been shown previously to contain overlapping amino acid sequences (R. McPherson and J. Rose, J. Virol. 46:523-529, 1983). In the present study we demonstrate definitively that these proteins are encoded in the right half of the adeno-asso...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.591-597.1984
更新日期:1984-11-01 00:00:00
abstract:UNLABELLED:JC polyomavirus (JCPyV) infection of immunocompromised individuals results in the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML). The viral capsid of JCPyV is composed primarily of the major capsid protein virus protein 1 (VP1), and pentameric arrangement of VP1 monomers results...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00089-15
更新日期:2015-04-01 00:00:00
abstract::The binding properties of seven CD4-blocking monoclonal antibodies raised against recombinant gp120 of human immunodeficiency virus type 1 strain MN (HIV-1MN) and two CD4-blocking monoclonal antibodies to recombinant envelope glycoproteins gp120 and gp160 of substrain IIIB of HIVLAI were analyzed. With a panel of reco...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.6179-6191.1993
更新日期:1993-10-01 00:00:00
abstract:UNLABELLED:Influenza virus transcription requires functional coupling with cellular transcription for the cap-snatching process. Despite this fact, RNA polymerase II (RNAP II) is degraded during infection in a process triggered by the viral polymerase. Reassortant viruses from the A/PR/8/34 (PR8) strain that induce (hv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02263-13
更新日期:2014-03-01 00:00:00
abstract:UNLABELLED:Triggering the amoebal phagocytosis process is a sine qua non condition for most giant viruses to initiate their replication cycle and consequently to promote their progeny formation. It is well known that the amoebal phagocytosis process requires the recognition of particles of >500 nm, and most amoebal gia...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00177-16
更新日期:2016-05-12 00:00:00
abstract::The phosphoprotein (P) of the nonsegmented negative-sense RNA viruses is a multimeric modular protein that is essential for RNA transcription and replication. Despite great variability in length and sequence, the architecture of this protein is conserved among the different viral families, with a long N-terminal intri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01789-19
更新日期:2020-02-28 00:00:00
abstract::The hepatitis B virus X protein (HBX) is essential for the establishment of HBV infection in vivo and exerts a pleiotropic effect on diverse cellular functions. The yeast two-hybrid system had indicated that HBX could interact with two subunits of the 26S proteasome. Here we demonstrate an association in vivo of HBX w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7231-7240.1999
更新日期:1999-09-01 00:00:00
abstract::T-cell subsets were studied by fluorescence-activated cell sorter analysis in 57 feline immunodeficiency virus (FIV)-seropositive cats with naturally acquired FIV infection to see whether CD4(+)-CD8+ alterations were comparable to those observed in human immunodeficiency virus-infected patients. CD4+ values were decre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.3.1484-1488.1992
更新日期:1992-03-01 00:00:00
abstract::The C-type lectins DC-SIGN and DC-SIGNR [collectively referred to as DC-SIGN(R)] bind and transmit human immunodeficiency virus (HIV) and simian immunodeficiency virus to T cells via the viral envelope glycoprotein (Env). Other viruses containing heavily glycosylated glycoproteins (GPs) fail to interact with DC-SIGN(R...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1337-1346.2003
更新日期:2003-01-01 00:00:00
abstract::A vigorous expansion of antigen-specific CD8(+) T cells lacking apparent effector function was observed in a rhesus macaque acutely infected with the simian immunodeficiency virus (SIV) strain SIVmac239. Antigen-specific CD8(+) T cells were identified using antigenic-peptide class I major histocompatibility complex te...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.6.3028-3033.2001
更新日期:2001-03-01 00:00:00