Abstract:
UNLABELLED:Triggering the amoebal phagocytosis process is a sine qua non condition for most giant viruses to initiate their replication cycle and consequently to promote their progeny formation. It is well known that the amoebal phagocytosis process requires the recognition of particles of >500 nm, and most amoebal giant viruses meet this requirement, such as mimivirus, pandoravirus, pithovirus, and mollivirus. However, in the context of the discovery of amoebal giant viruses in the last decade, Marseillevirus marseillevirus (MsV) has drawn our attention, because despite its ability to successfully replicate in Acanthamoeba, remarkably it does not fulfill the >500-nm condition, since it presents an ∼250-nm icosahedrally shaped capsid. We deeply investigated the MsV cycle by using a set of methods, including virological, molecular, and microscopic (immunofluorescence, scanning electron microscopy, and transmission electron microscopy) assays. Our results revealed that MsV is able to form giant vesicles containing dozens to thousands of viral particles wrapped by membranes derived from amoebal endoplasmic reticulum. Remarkably, our results strongly suggested that these giant vesicles are able to stimulate amoebal phagocytosis and to trigger the MsV replication cycle by an acidification-independent process. Also, we observed that MsV entry may occur by the phagocytosis of grouped particles (without surrounding membranes) and by an endosome-stimulated pathway triggered by single particles. Taken together, not only do our data deeply describe the main features of MsV replication cycle, but this is the first time, to our knowledge, that the formation of giant infective vesicles related to a DNA virus has been described. IMPORTANCE:Triggering the amoebal phagocytosis process is a sine qua non condition required by most giant viruses to initiate their replication cycle. This process requires the recognition of particles of >500 nm, and many giant viruses meet this requirement. However, MsV is unusual, as despite having particles of ∼250 nm it is able to replicate in Acanthamoeba Our results revealed that MsV is able to form giant vesicles, containing dozens to thousands of viral particles, wrapped in membranes derived from amoebal endoplasmic reticulum. Remarkably, our results strongly suggest that these giant vesicles are able to stimulate phagocytosis using an acidification-independent process. Our work not only describes the main features of the MsV replication cycle but also describes, for the first time to our knowledge, the formation of huge infective vesicles in a large DNA viruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Arantes TS,Rodrigues RA,Dos Santos Silva LK,Oliveira GP,de Souza HL,Khalil JY,de Oliveira DB,Torres AA,da Silva LL,Colson P,Kroon EG,da Fonseca FG,Bonjardim CA,La Scola B,Abrahão JSdoi
10.1128/JVI.00177-16subject
Has Abstractpub_date
2016-05-12 00:00:00pages
5246-55issue
11eissn
0022-538Xissn
1098-5514pii
JVI.00177-16journal_volume
90pub_type
杂志文章abstract::Studies of rotavirus morphogenesis, transport, and release have shown that although these viruses are released from the apical surface of polarized intestinal cells before cellular lysis, they do not follow the classic exocytic pathway. Furthermore, increasing evidence suggests that lipid rafts actively participate in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3935-3946.2006
更新日期:2006-04-01 00:00:00
abstract::We previously showed that murine polyomavirus mutants that lack both middle T (MT) and small T (ST) functions have a severe pleiotropic defect in early and late viral gene expression as well as genome amplification. The respective contribution of MT and ST to this phenotype was unclear. This work separates the roles o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00679-06
更新日期:2006-08-01 00:00:00
abstract::A procedure has been developed for the isolation of Newcastle disease virus (NDV) envelope proteins. The two surface glycoproteins and the non-glycosylated membrane protein were solubilized with 2% Triton X-100 and 1 m KCl. Removal of the KCl by dialysis yielded by precipitation a pure preparation of the non-glycosyla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.2.263-271.1973
更新日期:1973-02-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) epidemic in Southeast Asia has been largely due to the emergence of clade E (HIV-1E). It has been suggested that HIV-1E is derived from a recombinant lineage of subtype A (HIV-1A) and subtype E, with multiple breakpoints along the E genome. We obtained complete genome se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10752-10765.2000
更新日期:2000-11-01 00:00:00
abstract::Monoclonal antibody-resistant mutants have been widely used to estimate virus mutation frequencies. We demonstrate that standard virion neutralization inevitably underestimates monoclonal antibody-resistant mutant genome frequencies of vesicular stomatitis virus, due to phenotypic masking-mixing when wild-type (wt) vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5030-5036.1989
更新日期:1989-12-01 00:00:00
abstract::Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5' end, which are deleted during replication to generate authentic viral genomes. We removed those 5'-end extra nucleotides utilizing a hammerhead ribozy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6394-6400.2000
更新日期:2000-07-01 00:00:00
abstract::Monoclonal antibodies (MAbs) against the rubella virion were used to locate epitopes involved in hemagglutination and neutralization. The MAbs exhibiting high-level hemagglutination-inhibiting activity were shown by Western blot analysis to be specific for the E1 polypeptide; this is consistent with the presence of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.893-898.1986
更新日期:1986-03-01 00:00:00
abstract::Adeno-associated virus (AAV) is a single-stranded DNA virus which requires adenovirus as a helper for productive infection. We studied whether intracellular AAV DNA in KB cells was present in a chromatin-like structure by digesting infected cell nuclei with micrococcal nuclease. Virus DNA was detected by agarose gel e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.79-87.1983
更新日期:1983-10-01 00:00:00
abstract:UNLABELLED:The HIV-1 virion infectivity factor (Vif) targets the cellular cytidine deaminases APOBEC3G (A3G) and APOBEC3F (A3F) for degradation via the host ubiquitin-proteasome pathway. Vif recruits a cellular E3 ubiquitin ligase to polyubiquitinate A3G/F. The activity of Vif critically depends on the cellular core bi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03824-13
更新日期:2014-03-01 00:00:00
abstract::Influenza virus has a set of ribonucleoproteins (RNPs) consisting of viral RNAs, influenza virus polymerase subunits, and nucleoprotein. Intracellular reconstitution of the whole set of RNPs via plasmid transfection results in the generation of influenza virus. By the use of reverse genetics and dual promoters, we con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00781-09
更新日期:2009-09-01 00:00:00
abstract::Callitrichid hepatitis is an infection of New World primates caused by an arenavirus, currently referred to as callitrichid hepatitis virus, that is closely related to lymphocytic choriomeningitis virus (LCMV). We have cloned and sequenced the GP-C gene of callitrichid hepatitis virus and found that the cDNA sequence ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1349-1352.1995
更新日期:1995-02-01 00:00:00
abstract::Mus dunni endogenous virus (MDEV) is activated from cells of the Asian wild mouse M. dunni (also known as Mus terricolor) in response to treatment with either 5-iodo-2'-deoxyuridine or hydrocortisone. MDEV represents a new murine retrovirus interference group and thus appears to use a different receptor for entry into...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4663-4670.1997
更新日期:1997-06-01 00:00:00
abstract:UNLABELLED:The distribution of vesicular stomatitis virus (VSV) nucleocapsids in the cytoplasm of infected cells was analyzed by scanning confocal fluorescence microscopy using a newly developed quantitative approach called the border-to-border distribution method. Nucleocapsids were located near the cell nucleus at ea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00488-16
更新日期:2016-06-10 00:00:00
abstract::Myxoma virus (MV) encodes a cell surface protein (M135R) that is predicted to mimic the host alpha/beta interferon receptor (IFN-alpha/beta-R) and thus prevent IFN-alpha/beta from triggering a host antiviral response. This prediction is based on sequence similarity to B18R, the viral IFN-alpha/beta-R from vaccinia vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01633-06
更新日期:2007-01-01 00:00:00
abstract::Corneal infection with herpes simplex virus 1 (HSV-1) leads to infection of trigeminal ganglia (TG), typically followed by the establishment of latency in the infected neurons. When latency is disrupted, the virus reactivates and migrates back to the cornea, where it restimulates the immune response, leading to lesion...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00659-19
更新日期:2019-07-30 00:00:00
abstract::The Aichi virus 2A protein is not a protease, unlike many other picornavirus 2A proteins, and it is related to a cellular protein, H-rev107. Here, we examined the replication properties of two 2A mutants in Vero cells and a cell-free translation/replication system. In one mutant, amino acids 36 to 126 were replaced wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01051-08
更新日期:2008-10-01 00:00:00
abstract::The vaccinia virus H2R gene (VACWR 100) is conserved in all sequenced members of the poxvirus family and encodes a protein with a predicted transmembrane domain and four invariant cysteines. A recombinant vaccinia virus, in which expression of the H2 protein is stringently regulated, was unable to replicate without in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.4744-4754.2005
更新日期:2005-04-01 00:00:00
abstract:UNLABELLED:Influenza virus hemagglutinin (HA) mediates virus entry by binding to cell surface receptors and fusing the viral and endosomal membranes following uptake by endocytosis. The acidic environment of endosomes triggers a large-scale conformational change in the transmembrane subunit of HA (HA2) involving a loop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00939-15
更新日期:2015-10-01 00:00:00
abstract::Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01117-06
更新日期:2006-11-01 00:00:00
abstract::Spring viremia of carp virus (SVCV) is a highly pathogenic Vesiculovirus in the common carp. The phosphoprotein (P protein) of SVCV is a multifunctional protein that acts as a polymerase cofactor and an antagonist of cellular interferon (IFN) response. Here, we report the 1.5-Å-resolution crystal structure of the P pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00855-20
更新日期:2020-07-16 00:00:00
abstract::The 2009 pandemic influenza virus (pdm/09) has been frequently introduced to pigs and has reassorted with other swine viruses. Recently, H3N2 reassortants with pdm/09-like internal genes were isolated in Guangxi and Hong Kong, China. Genetic and epidemiological analyses suggest that these viruses have circulated in sw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06824-11
更新日期:2012-02-01 00:00:00
abstract::Five regions of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) have been shown to be important in the transcriptional regulation of HIV in HeLa cells. These include the negative regulatory, enhancer, SP1, TATA, and TAR regions. Previous studies in which purified SP1 was used showed that the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2585-2591.1989
更新日期:1989-06-01 00:00:00
abstract::The reticuloendotheliosis viruses (REVs), originally isolated from avian species, constitute a group of retroviruses which are more closely related to mammalian retroviruses than to other avian retroviruses. The envelope glycoproteins of members of the REV group display a striking amino acid sequence identity with a g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3448-3454.1992
更新日期:1992-06-01 00:00:00
abstract::Equine infectious anemia virus was found to be comprised of fourteen polypeptides of molecular weight ranging from 10,000 to 79,000. Eighty percent of the virion protein was accounted for by five polypeptides, including two non-glycosylated components (p29 and p13) comprising one-half of the virion protein and three g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.997-1001.1978
更新日期:1978-12-01 00:00:00
abstract::We have shown previously that processing of the Sindbis virus envelope protein precursor PE2 to envelope protein E2 is not required for virus maturation in cultured vertebrate fibroblast cells and that unprocessed PE2 can be incorporated into infectious virus in place of E2 (J. F. Presley and D. T. Brown, J. Virol. 63...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.1905-1909.1991
更新日期:1991-04-01 00:00:00
abstract::All hepatitis B viruses replicate by protein-primed reverse transcription, employing a specialized reverse transcriptase, P protein, that carries a unique terminal protein (TP) domain. To initiate reverse transcription, P protein must bind to a stem-loop, epsilon, on the pregenomic RNA template. TP then provides a Y r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01196-07
更新日期:2007-12-01 00:00:00
abstract::Rabies, caused by rabies virus (RABV), is a fatal encephalitis in humans and other mammals, which continues to present a public health threat in most parts of the world. Our previous study demonstrated that Toll-like receptor 7 (TLR7) is essential in the induction of anti-RABV antibodies via the facilitation of germin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00111-20
更新日期:2020-04-16 00:00:00
abstract:UNLABELLED:Giardia lamblia virus (GLV) is a small, nonenveloped, nonsegmented double-stranded RNA (dsRNA) virus infecting Giardia lamblia, the most common protozoan pathogen of the human intestine and a major agent of waterborne diarrheal disease worldwide. GLV (genus Giardiavirus) is a member of family Totiviridae, al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02745-14
更新日期:2015-01-15 00:00:00
abstract::The binding of cyclophilin A (CypA) to the human immunodeficiency virus type 1 (HIV-1) capsid protein (CA protein) is required soon after virus entry into natural target cells. In Jurkat T lymphocytes, disrupting CypA-CA interaction either by cyclosporine (Cs) treatment or by alteration (e.g., P90A) of the CA inhibits...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00682-09
更新日期:2009-11-01 00:00:00
abstract::Polyacrylamide gel electrophoresis and tryptic peptide fingerprint analysis of the proteins made in a cell-free system derived from L-cells and immunoprecipitated with simian virus 40 (SV40) anti-T serum demonstrated that both SV40 large-T and small-T antigens are synthesized in vitro in response to mRNA isolated from...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.154-170.1978
更新日期:1978-10-01 00:00:00