Abstract:
:Monoclonal antibody-resistant mutants have been widely used to estimate virus mutation frequencies. We demonstrate that standard virion neutralization inevitably underestimates monoclonal antibody-resistant mutant genome frequencies of vesicular stomatitis virus, due to phenotypic masking-mixing when wild-type (wt) virions are present in thousandsfold greater numbers. We show that incorporation of antibody into the plaque overlay medium (after virus penetration at 37 degrees C) can provide accurate estimates of genome frequencies of neutral monoclonal antibody-resistant mutant viruses in wt clones. By using this method, we have observed two adjacent G----A base transition frequencies in the I3 epitope to be of the order of 10(-4) in a wt glycine codon. This appears to be slightly lower than the frequencies observed at other sites for total (viable and nonviable) virus genomes when using a direct sequence approach.
journal_name
J Viroljournal_title
Journal of virologyauthors
Holland JJ,de la Torre JC,Steinhauer DA,Clarke D,Duarte E,Domingo Edoi
10.1128/JVI.63.12.5030-5036.1989subject
Has Abstractpub_date
1989-12-01 00:00:00pages
5030-6issue
12eissn
0022-538Xissn
1098-5514journal_volume
63pub_type
杂志文章abstract::Mutants (N(-)nin) of bacteriophage lambda in which the N gene product is not required for growth on wild-type Escherichia coli do not plate on recA bacterial mutants. Secondary mutants, selected for growth on recA, lie within the immunity region to the right of gene cI and appear identical to the cro mutants of Eisen ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.6.938-945.1972
更新日期:1972-06-01 00:00:00
abstract::The development of resistance to host defense mechanisms such as tumor necrosis factor (TNF)- and Fas-mediated apoptosis of transformed or virus-infected cells may be a critical component in the development of disease. To find genes that protect cells from apoptosis, we used an expression cloning strategy and identifi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7509-7517.1997
更新日期:1997-10-01 00:00:00
abstract::Multiple steps of the life cycle of hepatitis B virus (HBV) are known to be coupled to hepatic metabolism. However, the details of involvement of the hepatic metabolic milieu in HBV infection remain incompletely understood. Hepatic lipid metabolism is controlled by a complicated transcription factor network centered o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01771-17
更新日期:2018-03-28 00:00:00
abstract::Lysine-specific demethylase 1 (LSD1) targets cellular proteins, including histone H3, p53, E2F, and Dnmt1, and is involved in the regulation of gene expression, DNA replication, the cell cycle, and the DNA damage response. LSD1 catalyzes demethylation of histone H3K9 associated with herpes simplex virus 1 (HSV-1) imme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00974-20
更新日期:2020-09-15 00:00:00
abstract::The early proteins of simian virus 40 (SV40) large T and small t antigen (T/t antigen) can each cause the transcriptional activation of a variety of cellular and viral promoters. We showed previously that simian cellular DNA-binding factors (the Band A factors) bind to sequences within the SV40 late promoter which are...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.173-184.1990
更新日期:1990-01-01 00:00:00
abstract::Cytomegalovirus (CMV) is acquired by the oral route in children, and primary infection is associated with abundant mucosal replication, as well as the establishment of latency in myeloid cells that results in lifelong infection. The efficiency of primary CMV infection in humans following oral exposure, however, is unk...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00380-17
更新日期:2017-05-26 00:00:00
abstract::Tick-borne encephalitis virus (TBEV), a major tick-borne viral pathogen of humans, is known to cause neurological diseases such as meningitis, encephalitis, and meningoencephalitis. However, the life cycle and pathogenesis of TBEV are not well understood. Here, we show that the knockdown or knockout of ADAM15 (a disin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01926-20
更新日期:2021-01-28 00:00:00
abstract::The E6 oncoprotein of high-risk human papillomavirus type 16 (HPV16) interacts with several nuclear transcription factors and coactivators in addition to cytoplasmic proteins, suggesting that nuclear import of HPV16 E6 plays a role in the cellular transformation process. In this study we have investigated the nuclear ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2330-2337.2003
更新日期:2003-02-01 00:00:00
abstract::Recombination studies have established that retroviral long terminal repeats (LTRs) are important genetic determinants of the viral capacity to induce hematopoietic tumors and to specify the type of cell making up the tumor. Plasmids containing LTRs of several murine leukemia viruses linked to the chloramphenicol acet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.1067-1072.1987
更新日期:1987-04-01 00:00:00
abstract::The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters em...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01259-13
更新日期:2013-09-01 00:00:00
abstract::Sequencing and reversion analysis of murine hepatitis virus (MHV) temperature-sensitive (ts) viruses has identified putative ts mutations in the replicase nonstructural proteins (nsp's) of these coronaviruses. In this study, reverse transcriptase PCR sequencing of the RNA genome of an isolate of the MHV ts virus Alb t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00203-08
更新日期:2008-06-01 00:00:00
abstract::The beginning of the second century of research in the field of virology (the first virus was discovered in 1898) was marked by its amalgamation with bioinformatics, resulting in the birth of a new domain--viroinformatics. The availability of more than 100 Web servers and databases embracing all or specific viruses (f...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.02027-14
更新日期:2015-02-01 00:00:00
abstract::Understanding the role of host factors during lethal influenza virus infection is critical to deciphering the events that determine the fate of the host. One such factor is encoded by the Mx1 gene, which confers resistance to influenza virus infection. Here, we compared pathology and global gene expression profiles in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06156-11
更新日期:2012-03-01 00:00:00
abstract::Human foamy virus can establish persistent infections in human hematopoietic cell lines, such as H92.1.7 (erythroblastoid cells), Jurkat (CD4+ T cells), and U937 (myeloid-monocytic cells). The infection is characterized by constant production of infectious viruses (for > 2 1/2 years) with no cytopathic effects on the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.1250-1254.1996
更新日期:1996-02-01 00:00:00
abstract::The potential use of the modified vaccinia virus Ankara (MVA) strain as a live recombinant vector to deliver antigens and elicit protective immune responses against infectious diseases demands a comprehensive understanding of the effect of MVA infection on human host gene expression. We used microarrays containing mor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.11.5820-5834.2004
更新日期:2004-06-01 00:00:00
abstract::The relationship between NS protein phosphorylation and RNA polymerase activities was determined in nucleocapsids purified from vesicular stomatitis virus grown in BHK cells. Phosphate incorporation into endogenous NS protein under transcription conditions reached a maximum value of 0.06 mol/mol of NS within 20 to 30 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3259-3264.1990
更新日期:1990-07-01 00:00:00
abstract::The TAATGARAT motif in the herpes simplex virus (HSV) immediate-early (IE) gene promoters plays a key role in their activation by the Oct-1-Vmw65 complex, but its role in mediating inhibitory effects of cellular octamer-binding proteins is less clear. We have used indicator viruses containing reporter constructs with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3495-3500.1998
更新日期:1998-04-01 00:00:00
abstract::To assess the possible contribution of host immune responses to the exertion of Fv2-associated resistance to Friend virus (FV)-induced disease development, we inoculated C57BL/6 (B6) mice that lacked various subsets of lymphocytes with FV containing no lactate dehydrogenase-elevating virus. Fv2(r) B6 mice lacking CD4(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02506-13
更新日期:2013-12-01 00:00:00
abstract::Infection by viruses depends on a balance between capsid stability and dynamics. This study investigated biologically and biotechnologically relevant aspects of the relationship in foot-and-mouth disease virus (FMDV) between capsid structure and thermostability and between thermostability and infectivity. In the FMDV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02293-18
更新日期:2019-05-01 00:00:00
abstract::Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1108-1114.1974
更新日期:1974-11-01 00:00:00
abstract::We have used deletion and recombinant long terminal repeat (LTR) mutants to examine enhancer activity differences between LTRs of the nonpathogenic Akv and the thymus lymphomagenic MCF13 murine retroviruses. Deletion mutant analysis revealed that major control regions for MCF13 and Akv LTR enhancer activity were simil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3353-3361.1989
更新日期:1989-08-01 00:00:00
abstract::Infectious leukemia virus production by two chronically infected NIH/MOL lines was strongly inhibited by interferon treatment of the cells. The corresponding degree of inhibition in JLSV-11 cells was much lower. Multiplication of encephalomyocarditis virus in all three cell lines was barely affected by interferon trea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.3.1017-1027.1983
更新日期:1983-03-01 00:00:00
abstract::Recent studies have explored the chromatin structures associated with the herpes simplex virus type 1 (HSV-1) genome during latency, particularly with regard to specific histone tail modifications such as acetylation and dimethylation. The objective of our present study was to develop a rapid systemic method of in viv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00070-07
更新日期:2007-06-01 00:00:00
abstract::Sequence analysis of the short unique (Us) segment of the bovine herpesvirus 1 (BHV-1) genome predicted that the Us open reading frame (ORF) 4 encodes a protein with homology to glycoprotein G (gG) of other alpha-herpesviruses (P. Leung-Tack, J.-C. Audonnet, and M. Riviere, Virology 199:409-421, 1994). RNA analysis sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3032-3038.1996
更新日期:1996-05-01 00:00:00
abstract::R325-beta TK+, a herpes simplex virus 1 mutant carrying a 500-base-pair deletion in the alpha 22 gene and the wild-type (beta) thymidine kinase (TK) gene, was previously shown to grow efficiently in HEp-2 and Vero cell lines. We report that in rodent cell lines exemplified by the Rat-1 line, plating efficiency was red...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.2.338-346.1985
更新日期:1985-08-01 00:00:00
abstract::A virus structurally similar to viruses associated with maedi, progressive pneumonia, and visna of sheep has been isolated from buffy coat cells of cattle with chronic lymphocytosis. Electron microscope studies revealed three variants of the virion: (i) an intracytoplasmic form 98 to 116 nm in diameter when occurring ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.197-204.1974
更新日期:1974-01-01 00:00:00
abstract::Many steps along the herpesvirus assembly and maturation pathway remain unclear. In particular, the acquisition of the virus tegument is a poorly understood process, and the molecular interactions involved in tegument assembly have not yet been defined. Previously we have shown that the two major herpes simplex virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.13082-13093.2005
更新日期:2005-10-01 00:00:00
abstract::To gain further insight on the function of the herpes simplex virus type 1 (HSV-1) packaging signal (a sequence), we constructed a recombinant virus containing a unique a sequence, which was flanked by two loxP sites in parallel orientation. The phenotype of this recombinant, named HSV-1 LaL, was studied in cell lines...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8402-8412.2000
更新日期:2000-09-01 00:00:00
abstract::The Epstein-Barr virus (EBV) genome contains an open reading frame, BHRF1, that encodes a presumptive membrane protein with sequence similarity to the proto-oncogene bcl2, which is linked to human B-cell follicular lymphoma. Potential roles for BHRF1 in EBV's ability to growth transform human B cells and to replicate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.1899-1906.1992
更新日期:1992-04-01 00:00:00
abstract::A distinctive feature of chronic human immunodeficiency virus type 1 (HIV-1) infection is the presence of multiple coexisting genetic variants, or subpopulations, that comprise the HIV-1 population detected in the peripheral blood. Analysis of HIV-1 RNA decay dynamics during the initiation of highly active antiretrovi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02486-08
更新日期:2009-05-01 00:00:00