Abstract:
:Understanding the role of host factors during lethal influenza virus infection is critical to deciphering the events that determine the fate of the host. One such factor is encoded by the Mx1 gene, which confers resistance to influenza virus infection. Here, we compared pathology and global gene expression profiles in lung tissue from BALB/c (Mx1(-)) and BALB · A2G-Mx1 mice (Mx1(+/+)) infected with the fully reconstructed 1918 pandemic influenza virus. Mx1(+/+) mice showed less tissue damage than Mx(-) animals, and pathology and mortality were further reduced by treating the mice with interferon prior to infection. Using global transcriptional profiling, we identified distinct molecular signatures associated with partial protection, complete protection, and the contribution of interferon to the host response. In the absence of interferon treatment, partial protection was characterized by the generation of an acute response with the upregulation of genes associated with apoptosis, reactive oxygen species, and cell migration. Complete protection was characterized by the downregulation of cytokine and chemokine genes previously associated with influenza virus pathogenesis. The contribution of interferon treatment to total protection in virus-infected Mx1(+/+) mice was characterized by the altered regulation of cell cycle genes. These genes were upregulated in Mx1(+/+) mice treated with interferon but downregulated in the absence of interferon treatment. Our results suggest that Mx1(+/+) mice generate a protective antiviral response by controlling the expression of key modulator molecules associated with influenza virus lethality.
journal_name
J Viroljournal_title
Journal of virologyauthors
Cilloniz C,Pantin-Jackwood MJ,Ni C,Carter VS,Korth MJ,Swayne DE,Tumpey TM,Katze MGdoi
10.1128/JVI.06156-11subject
Has Abstractpub_date
2012-03-01 00:00:00pages
2437-46issue
5eissn
0022-538Xissn
1098-5514pii
JVI.06156-11journal_volume
86pub_type
杂志文章abstract::To examine the cell fusion activity of hepatitis C virus (HCV) envelope proteins (E1 and E2), we have established a sensitive cell fusion assay based on the activation of a reporter gene as described previously (O. Nussbaum, C. C. Broder, and E. A. Berger, J. Virol. 68:5411-5422, 1994). The chimeric HCV E1 and E2 prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5066-5074.2000
更新日期:2000-06-01 00:00:00
abstract::Investigation of the human antibody response to the 1957 pandemic H2N2 influenza A virus has been largely limited to serologic studies. We generated five influenza virus hemagglutinin (HA)-reactive human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of healthy donors who were born betw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.07158-11
更新日期:2012-06-01 00:00:00
abstract::We set out to test the hypothesis that interleukin-22 (IL-22), a cytokine crucial for epithelial cell homeostasis and recovery from tissue injury, would be protective during influenza virus infection. Recent studies have identified phenotypically and functionally unique intestinal NK cells capable of producing the cyt...
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pub_type: 杂志文章
doi:10.1128/JVI.00187-10
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.656-661.1984
更新日期:1984-09-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) is the causative agent for Kaposi sarcoma (KS), primary effusion lymphoma (PEL), and a subset of multicentric Castleman disease (MCD). The KSHV life cycle has two principal gene repertoires, latent and lytic. KSHV viral interleukin-6 (vIL-6), an analog of human ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01192-15
更新日期:2015-10-21 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3139-3143.1990
更新日期:1990-06-01 00:00:00
abstract::We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M. Binley, R. W. Sanders, B. Clas, N. Schuelke, A. Master, Y. Guo, F. Kajumo, D. J. Anselma, P. J. Maddon, W. C. Olson, and J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5678-5684.2003
更新日期:2003-05-01 00:00:00
abstract::Gene expression of human immunodeficiency virus (HIV) is modulated by both cellular transcription factors, which bind to cis-acting regulatory elements in the HIV-1 long terminal repeat (LTR) and the viral transactivator, tat. The enhancer element in the HIV-1 LTR which extends from -103 to -82 is critical for gene ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1002-1009.1994
更新日期:1994-02-01 00:00:00
abstract::Varicella-zoster virus (VZV) is the causative agent of chickenpox and herpes zoster (shingles). After the primary infection, the virus remains latent in sensory ganglia and reactivates upon weakening of the cellular immune system due to various conditions, erupting from sensory neurons and infecting the corresponding ...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
abstract::A number of eucaryotic viruses have devised strategies to minimize the deleterious effects on protein synthesis caused by activation of the interferon-induced, double-stranded-RNA-activated protein kinase, P68. In a recent report, we described the down regulation of the P68 protein kinase in cells infected by human im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.632-640.1991
更新日期:1991-02-01 00:00:00
abstract::Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan mic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00230-15
更新日期:2015-07-01 00:00:00
abstract::SER virus, a paramyxovirus that is closely related to simian virus 5 (SV5), is unusual in that it fails to induce syncytium formation. The SER virus F protein has an unusually long cytoplasmic tail (CT), and it was previously observed that truncations or specific mutations of this domain result in enhanced syncytium f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8513-8523.2004
更新日期:2004-08-01 00:00:00
abstract::From purified linear and superhelical DNAs, the restriction endonuclease maps of four xenotropic murine leukemia virus DNAs from NFS, NZB, BALB/c, and AKR mice were determined with ten restriction endonucleases. Each xenotropic proviral DNA was found to be a unique restriction endonuclease map, with differences in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.963-970.1981
更新日期:1981-12-01 00:00:00
abstract::Marek's disease virus (MDV) is a herpesvirus of chickens that induces T lymphomas and tumors within 4 to 5 weeks of infection. Although the ability of MDV to induce tumors was demonstrated many years ago and although a number of viral oncogenic proteins have been identified, the mechanism by which the MDV is implicate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5985-5996.2003
更新日期:2003-05-01 00:00:00
abstract::A map of bacteriophage lambda was constructed, including accurate positions for all 41 cut sites made by 12 different restriction enzymes. Over 100 fragments from single, multiple, and partial enzyme digestions were measured versus standards that were calibrated with respect to DNA molecules of known sequence. The dat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.390-400.1980
更新日期:1980-01-01 00:00:00
abstract::Herpesviruses encode a set of core proteins essential for lytic replication of their genomes. Three of these proteins form a tripartite helix-primase complex that, in the case of Epstein-Barr virus (EBV), consists of the helicase BBLF4, the primase BSLF1, and the linker protein BBLF2/3. BBLF2/3 and its homologs in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.245-256.2005
更新日期:2005-01-01 00:00:00
abstract::We have reported previously that ocular infection of different strains of mice with recombinant herpes simplex virus 1 (HSV-1) constitutively expressing interleukin-2 (IL-2) provokes central nervous system (CNS) demyelination and optic neuropathy, as determined by changes in visual evoked cortical potentials and patho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02241-13
更新日期:2013-11-01 00:00:00
abstract::Two of 25 healthy pet sooty mangabey (SM) monkeys (Cercocebus atys) living in West Africa were seropositive by immunoblot when surveyed for antibody to simian immunodeficiency virus of macaques (SIVmac). SIVsmLIB1 was isolated from one of the pet sooty mangabeys. Nucleotide sequence data showed that this isolate is a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4480-4485.1991
更新日期:1991-08-01 00:00:00
abstract::In this study, we investigated the interaction of reovirus particles with cell membranes by using a 51Cr release assay. We confirmed prior observations (J. Borsa, B. D. Morash, M. D. Sargent, T. P. Copps, P. A. Lievaart, and J. G. Szekely, J. Gen. Virol. 45:161-170, 1979) that intermediate subviral particles (ISVPs) o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5339-5345.1993
更新日期:1993-09-01 00:00:00
abstract::Duck embryo fibroblast (DEF) and chicken embryo fibroblast (CEF) cultures infected with Marek's disease virus were studied by combined fluorescent antibody and electron microscopy techniques. In both DEF and CEF cultures, cells containing immunofluorescent (IF) antigen also contained herpesvirus particles; conversely,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.1.79-90.1970
更新日期:1970-01-01 00:00:00
abstract::The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.6.1254-1262.1974
更新日期:1974-06-01 00:00:00
abstract::Experiments were conducted to investigate the roles of Marek's disease virus serotype 1 (MDV-1) major tegument proteins VP11/12, VP13/14, VP16, and VP22 in viral growth in cultured cells. Based on a bacterial artificial chromosome clone of MDV-1 (BAC20), mutant viruses were constructed in which the MDV-1 homologs of U...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1959-1970.2002
更新日期:2002-02-01 00:00:00
abstract::Purified retrovirus Gag proteins can assemble in vitro into virus-like particles (VLPs) in the presence of RNA. It was shown previously that a Rous sarcoma virus Gag protein missing only the protease domain forms spherical particles resembling immature virions lacking a membrane but that a similar protein missing the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.21.10260-10268.2000
更新日期:2000-11-01 00:00:00
abstract::Defensins are innate immune effector peptides expressed at mucosal surfaces throughout the human body and are potently antiviral in vitro The role of defensins in viral pathogenesis in vivo is poorly understood; however, recent studies have revealed that defensin-virus interactions in vivo are complicated and distinct...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00904-15
更新日期:2016-05-12 00:00:00
abstract::Human immunodeficiency virus type 1 Tat exerts prominent angiogenic effects which may lead to a variety of vasculopathic conditions in AIDS patients. Because endothelial cells undergo prominent cytoskeletal rearrangement during angiogenesis, we investigated the specific effects of Tat on the endothelial cell actin cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.779-789.2004
更新日期:2004-01-01 00:00:00
abstract::We have developed a reconstituted system which models the events associated with human immunodeficiency virus type 1 (HIV-1) plus-strand transfer. These events include synthesis of plus-strand strong-stop DNA [(+) SSDNA] from a minus-strand DNA donor template covalently attached to human tRNA3Lys, tRNA primer removal,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4794-4805.1999
更新日期:1999-06-01 00:00:00
abstract::Homologous recombination is an important biological process that facilitates genome rearrangement and repair of DNA double-strand breaks (DSBs). The induction of Epstein-Barr virus (EBV) lytic replication induces ataxia telangiectasia-mutated (ATM)-dependent DNA damage checkpoint signaling, leading to the clustering o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00049-09
更新日期:2009-07-01 00:00:00
abstract::The human cytomegalovirus (HCMV) tegument protein pp71, encoded by gene UL82, stimulates viral immediate-early (IE) transcription. pp71 interacts with the cellular protein hDaxx at nuclear domain 10 (ND10) sites, resulting in the reversal of hDaxx-mediated repression of viral transcription. We demonstrate that pp71 di...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01215-08
更新日期:2008-12-01 00:00:00
abstract::Immunogenic regions of the gp41 transmembrane protein of human immunodeficiency virus type 1 (HIV-1) were previously mapped by examining polyclonal sera from HIV-infected patients and rodent polyclonal and monoclonal antibodies (MAbs) to peptides of gp41. To define the epitopes within these regions to which infected h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4832-4838.1991
更新日期:1991-09-01 00:00:00
abstract::A series of plasmids containing hepatitis A virus (HAV) cDNA was constructed such that positive-strand HAV RNA could be transcribed with T7 RNA polymerase. The plasmids differed in the number of 5'-terminal nucleotides representing the junctions between vectors and HAV sequences that were present in the transcripts. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2757-2760.1991
更新日期:1991-05-01 00:00:00