Abstract：
:SER virus, a paramyxovirus that is closely related to simian virus 5 (SV5), is unusual in that it fails to induce syncytium formation. The SER virus F protein has an unusually long cytoplasmic tail (CT), and it was previously observed that truncations or specific mutations of this domain result in enhanced syncytium formation. In addition to the long CT, the SER F protein has nine amino acid differences from the F protein of SV5. We previously observed only a partial suppression of fusion in a chimeric SV5 F protein with a CT derived from SER virus, indicating that these other amino acid differences between the SER and SV5 F proteins also play a role in regulating the fusion phenotype. To examine the effects of individual amino acid differences, we mutated the nine SER residues individually to the respective residues of the SV5 F protein. We found that most of the mutants were expressed well and were transported to the cell surface at levels comparable to that of the wild-type SER F protein. Many of the mutants showed enhanced lipid mixing, calcein transfer, and syncytium formation even in the presence of the long SER F protein CT. Some mutants, such as the I310 M, T438S, M489I, T516V, and N529K mutants, also showed fusion at lower temperatures of 32, 25, and 18 degrees C. The residue Asn529 plays a critical role in the suppression of fusion activity, as the mutation of this residue to lysine caused a marked enhancement of fusion. The effect of the N529K mutation on the enhancement of fusion by a previously described mutant, L539,548A, as well as by chimeric SV5/SER F proteins was also dramatic. These results indicate that activation to a fusogenic conformation is dependent on the interplay of residues in the ectodomain, the transmembrane domain, and the CT domain of paramyxovirus F proteins.
journal_name
J Viroljournal_title
Journal of virologyauthors
Seth S,Goodman AL,Compans RWdoi
10.1128/JVI.78.16.8513-8523.2004subject
Has Abstractpub_date
2004-08-01 00:00:00pages
8513-23issue
16eissn
0022-538Xissn
1098-5514pii
78/16/8513journal_volume
78pub_type
杂志文章abstract：:The interferon response elicited by Salmonella typhimurium mutants in mice is not dependent on the presence of a complete cell wall lipopolysaccharide. In fact, a mutant (G30/C21) which has lost all the polysaccharide side chains and sugars of the O antigen and contains only 2-keto-3-deoxyoctonate and lipid is indisti...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.1.6.1164-1167.1967
更新日期：1967-12-01 00:00:00
abstract：:We are exploring the use of interferon (IFN) genes for somatic cell gene therapy and have investigated the possibility of transforming cells into low constitutive IFN producers over prolonged periods of time without impeding cell survival or replication. Here we report the possibility of conferring a permanent antivir...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.65.2.664-671.1991
更新日期：1991-02-01 00:00:00
abstract：:The conversion of simian virus 40 (SV40) component II deoxyribonucleic acid to component I has been used to assay polynucleotide ligase in extracts of tissue culture cells. All cell types examined, including chicken, hamster, mouse, monkey, and human cells, contained adenosine triphosphate-dependent ligase. After infe...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.4.5.719-726.1969
更新日期：1969-11-01 00:00:00
abstract：:Phylogenetic analyses demonstrate significant diversity in worldwide circulating strains of human immunodeficiency virus type 1 (HIV-1). Detailed studies have revealed a complex pattern of intersubtype recombinations, as well as evidence of sub-subtypes circulating in various populations. In this study, we characteriz...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.78.22.12438-12445.2004
更新日期：2004-11-01 00:00:00
abstract：:The role of nitric oxide after poliovirus infection of the human HeLa (carcinoma) and U937 (promonocytic) cell lines has been analyzed. Both types of cells produced detectable levels of nitric oxide after poliovirus infection. However, this production was not sufficient to limit viral productivity. On the other hand, ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.3.2538-2540.1998
更新日期：1998-03-01 00:00:00
abstract：:Resistance or susceptibility of various mouse strains to central nervous system disease caused by different strains of coronavirus is well known. Data from the present study draw attention to an additional, genetically determined mechanism controlling CV infections. The resistance to A59 and JHM virus (JHMV) associate...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.62.9.3371-3377.1988
更新日期：1988-09-01 00:00:00
abstract：:The bovine leukemia virus (BLV) is an oncogenic retrovirus that is associated with the development of persistent lymphocytosis (PL) and lymphoma in cattle. While B lymphocytes have been shown to be the primary cellular target of BLV, recent studies suggest that some T lymphocytes and monocytes may be infected by the v...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.70.4.2178-2183.1996
更新日期：1996-04-01 00:00:00
abstract：:Type 3 reovirus inhibits L cell DNA synthesis, whereas type 1 reovirus exerts little or no effect on L cell DNA synthesis. By using recombinant viruses containing both type 1 and type 3 double-standard RNA segments, we determined that one double-stranded RNA segment, the reovirus type 3 S1 double-stranded RNA segment ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.38.1.389-392.1981
更新日期：1981-04-01 00:00:00
abstract：:Woodchuck hepatitis virus (WHV) mutants with core internal deletions (CID) occur naturally in chronically WHV-infected woodchucks, as do hepatitis B virus mutants in humans. We studied the replication of WHV deletion mutants in primary woodchuck hepatocyte cultures and in vivo after transmission to naive woodchucks. B...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.75.8.3811-3818.2001
更新日期：2001-04-01 00:00:00
abstract：:Primary human embryonic kidney (HEK) cells were transformed by a focus assay with BK virus (BKV) DNA molecularly cloned at its unique EcoRI site. Both viral DNA sequences and viral tumor antigens were present and expressed in all the foci that we examined. However, cells isolated from foci were incapable of growth in ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.58.2.680-683.1986
更新日期：1986-05-01 00:00:00
abstract：:We describe respiratory syncytial virus (RSV)-specific cytotoxic T-cell (CTL) lines and clones developed from the spleens of C57BL/6 and BALB/c mice. Line 7 and clones derived from it were H-2Kb restricted, whereas line 12 had both Kb and Db components. Both lines, and all the clones except one, could lyse targets inf...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.65.8.4494-4497.1991
更新日期：1991-08-01 00:00:00
abstract：:Understanding the correlates of immune protection against human immunodeficiency virus and simian immunodeficiency virus (SIV) will require defining the entire cellular immune response against the viruses. Here, we define two novel translation products from the SIV env mRNA that are targeted by the T-cell response in ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00138-09
更新日期：2009-10-01 00:00:00
abstract：:High-risk human papillomavirus type 16 (HPV-16) and HPV-18 are associated with the majority of human cervical carcinomas, and two viral genes, HPV E6 and E7, are commonly found to be expressed in these cancers. The presence of HPV-16 E7 is sufficient to induce epidermal hyperplasia and epithelial tumors in transgenic ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.71.8.5905-5914.1997
更新日期：1997-08-01 00:00:00
abstract：:The DNAs of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses contain overlapping segments of the early region of wild-type SV40 DNA. The complementary DNA strands of these five viruses have been separated with synthetic polyribonucleotides in isopycnic cesium chloride gradients. The relat...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.13.3.677-689.1974
更新日期：1974-03-01 00:00:00
abstract：:In 2010 and 2011, several devastating Newcastle disease (ND) outbreaks occurred in China, affecting broilers, layers, and breeders. The CK-JSX1-201005 virus was isolated from broiler breeder flocks vaccinated with the classical ND virus (NDV) vaccine program, but laying rate decreased from 80% to 30 to 40% in the clin...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02314-12
更新日期：2012-11-01 00:00:00
abstract：:Intracisternal-A-particle-related envelope-encoding (IAPE) proviral elements in the mouse genome encode and express an envelope-like protein that may allow transmission of IAPEs as infectious agents. To test IAPE mobility and potential transmission in mice, we have analyzed the distribution of IAPE elements in the gen...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.70.9.6450-6454.1996
更新日期：1996-09-01 00:00:00
abstract：UNLABELLED:Varicella-zoster virus (VZV) is an alphaherpesvirus that causes varicella and herpes zoster. Membrane fusion is essential for VZV entry and the distinctive syncytium formation in VZV-infected skin and neuronal tissue. Herpesvirus fusion is mediated by a complex of glycoproteins gB and gH-gL, which are necess...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00792-16
更新日期：2016-07-27 00:00:00
abstract：:Hepatitis C virus (HCV) often causes a persistent infection associated with hypergammaglobulinemia, high levels of antiviral antibody and circulating immune complexes, and immune complex disease. We previously reported that only a limited neutralizing activity to vesicular stomatitis virus or HCV pseudotype is generat...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01867-07
更新日期：2008-03-01 00:00:00
abstract：:Radioactive DNA complementary to nucleotide sequences in Moloney murine sarcoma virus (MSV) and Moloney leukemia virus (M-MuLV) complex was made by the endogenous reverse transcriptase reaction. These virus stocks contained a threefold excess of MSV over M-MuLV as measured by biological assay. The complementary DNA wa...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.18.2.481-490.1976
更新日期：1976-05-01 00:00:00
abstract：:P-element-induced wimpy-like (Piwil) proteins restrict the replication of mobile genetic elements in the germ line. They are also expressed in many transformed cell lines. In this study, we discovered that the human Piwil 2 (Hili) protein can also inhibit HIV replication, especially in activated CD4+ T cells that are ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00237-17
更新日期：2017-05-12 00:00:00
abstract：:We have mapped the temperature-sensitive (ts) lesions of three mutants, ts51, ts53, and ts65, and two other mutants, ts7 and ts20, to regions on the vaccinia virus genome that encode the 147- and 22-kilodalton subunits of the viral DNA-dependent RNA polymerase, respectively. Plasmid and bacteriophage clones from the H...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.63.2.705-713.1989
更新日期：1989-02-01 00:00:00
abstract：:Human enterovirus 71 (EV-71) is one of the major etiologic causes of hand, foot, and mouth disease (HFMD) among young children worldwide, with fatal instances of neurological complications becoming increasingly common. Global VP1 capsid sequences (n = 628) sampled over 4 decades were collected and subjected to compreh...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01019-09
更新日期：2010-04-01 00:00:00
abstract：:Human cytomegalovirus (HCMV) is a ubiquitously distributed pathogen that causes severe disease in immunosuppressed patients and newborn infants infected in utero. The viral envelope glycoprotein B (gB) is an attractive molecule for active vaccination and passive immunoprophylaxis and therapy. Using human monoclonal an...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00434-13
更新日期：2013-08-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) strains can be separated into genetic subtypes based on phylogenetic analysis of the envelope gene. Once it had been shown that population-wide intrasubtype genetic variation of HIV-1 strains increases in the course of the AIDS epidemic, it remained uncertain whether HIV-1 s...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.71.9.6332-6338.1997
更新日期：1997-09-01 00:00:00
abstract：:Extracts prepared from either mouse cells or monkey cells were examined for the ability to support in vitro bovine papillomavirus type 1 (BPV1) DNA replication, and they were used in parallel as a source of host replication proteins for affinity chromatography. DNA synthesis exhibited an absolute requirement for BPV1 ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.4.2341-2350.1995
更新日期：1995-04-01 00:00:00
abstract：:Human cytomegalovirus (HCMV) encodes four putative G protein-coupled receptors, including pUL78, whose rodent orthologues are known to be important for replication and spread in their hosts. To investigate the mechanism by which pUL78 contributes to viral replication and pathogenesis, we generated a derivative of the ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.05900-11
更新日期：2012-11-01 00:00:00
abstract：:Simian varicella virus (SVV) and varicella-zoster virus (VZV) are closely related alphaherpesviruses that cause varicella (chickenpox) in nonhuman primates and humans, respectively. After resolution of the primary disease, SVV and VZV establish latent infection of neural ganglia and may later reactivate to cause a sec...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00407-07
更新日期：2007-08-01 00:00:00
abstract：:VP16-H is an essential structural protein of herpes simplex virus type 1 (HSV-1) and is also a potent activator of virus immediate-early (IE) gene expression. Current models of functional determinants within VP16-H indicate that it consists of two domains, an N-terminal domain involved in recruiting VP16-H to a multic...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.74.21.10112-10121.2000
更新日期：2000-11-01 00:00:00
abstract：:A more complete assessment of ovine prion strain diversity will be achieved by complementing biological strain typing in conventional and ovine PrP transgenic mice with a biochemical analysis of the resultant PrPSc. This will provide a correlation between ovine prion strain phenotype and the molecular nature of differ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01454-08
更新日期：2008-11-01 00:00:00
abstract：:SF162 is a primary (PR), non-syncytium-inducing, macrophagetropic human immunodeficiency virus type 1 (HIV-1) clade B isolate which is resistant to antibody-mediated neutralization. Deletion of the first or second hypervariable envelope gp120 region (V1 or V2 loop, respectively) of this virus does not abrogate its abi...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.10.7840-7845.1998
更新日期：1998-10-01 00:00:00
