Abstract:
:Woodchuck hepatitis virus (WHV) mutants with core internal deletions (CID) occur naturally in chronically WHV-infected woodchucks, as do hepatitis B virus mutants in humans. We studied the replication of WHV deletion mutants in primary woodchuck hepatocyte cultures and in vivo after transmission to naive woodchucks. By screening 14 wild-caught, chronically WHV-infected woodchucks, two woodchucks, WH69 and WH70, were found to harbor WHV CID mutants. Consistent with previous results, WHV CID mutants from both animals had deletions of variable lengths (90 to 135 bp) within the middle of the WHV core gene. In woodchuck WH69, WHV CID mutants represented a predominant fraction of the viral population in sera, normal liver tissues, and to a lesser extent, in liver tumor tissues. In primary hepatocytes of WH69, the replication of wild-type WHV and CID mutants was maintained at least for 7 days. Although WHV CID mutants were predominant in fractions of cellular WHV replicative intermediates, mutant covalently closed circular DNAs (cccDNAs) appeared to be a small part of cccDNA-enriched fractions. Analysis of cccDNA-enriched fractions from liver tissues of other woodchucks confirmed that mutant cccDNA represents only a small fraction of the total cccDNA pool. Four naive woodchucks were inoculated with sera from woodchuck WH69 or WH70 containing WHV CID mutants. All four woodchucks developed viremia after 3 to 4 weeks postinoculation (p.i.). They developed anti-WHV core antigen (WHcAg) antibody, lymphoproliferative response to WHcAg, and anti-WHV surface antigen. Only wild-type WHV, but no CID mutant, was found in sera from these woodchucks. The WHV CID mutant was also not identified in liver tissue from one woodchuck sacrificed in week 7 p.i. Three remaining woodchucks cleared WHV. Thus, the presence of WHV CID mutants in the inocula did not significantly change the course of acute self-limiting WHV infection. Our results indicate that the replication of WHV CID mutants might require some specific selective conditions. Further investigations on WHV CID mutants will allow us to have more insight into hepadnavirus replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lu M,Hilken G,Yang D,Kemper T,Roggendorf Mdoi
10.1128/JVI.75.8.3811-3818.2001subject
Has Abstractpub_date
2001-04-01 00:00:00pages
3811-8issue
8eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
杂志文章abstract::Bacteriophage CL31 was isolated on a Corynebacterium lilium strain. Out of 30 strains tested, only CL31 was able to form plaques on Corynebacterium glutamicum ATCC 13287, Brevibacterium lactofermentum ATCC 21086, and Arthrobacter sp. strain SI55, but at a very low frequency. This phage belongs to group B of Bradley's ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1540-1545.1987
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abstract::White root rot, caused by the ascomycete Rosellinia necatrix, is a devastating disease worldwide, particularly in fruit trees in Japan. Here we report on the biological and molecular properties of a novel bipartite double-stranded RNA (dsRNA) virus encompassing dsRNA-1 (8,931 bp) and dsRNA-2 (7,180 bp), which was isol...
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journal_title:Journal of virology
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doi:10.1128/JVI.45.1.18-26.1983
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journal_title:Journal of virology
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doi:10.1128/JVI.44.3.1056-1067.1982
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pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
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journal_title:Journal of virology
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doi:10.1128/JVI.69.8.4994-5010.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.847-850.1988
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.8277-8281.1994
更新日期:1994-12-01 00:00:00
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journal_title:Journal of virology
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更新日期:1995-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00