Abstract:
:Precore and core proteins are two related co-carboxy-terminal proteins of hepatitis B virus. Precore protein contains the entire sequence of core protein plus an amino-terminal extension of 29 amino acid residues. Both proteins can display a common antigenic determinant known as core antigen (HBcAg). Clinically, HBcAg is detected in the nucleus, cytoplasm, or both of hepatitis B virus-infected hepatocytes. In order to understand the mechanism that regulates nuclear transport of HBcAg, various portions of precore and core proteins were linked to a reporter protein, human alpha-globin, and expressed in mammalian cells. Our results indicate that the precore protein-specific sequence, although important for nuclear transport, does not contain a nuclear localization signal. Instead, a signal for nuclear transport is located near the carboxy termini of precore and core proteins in the arginine-rich domain. This signal is made up of a set of two direct PRRRRSQS repeats and is highly conserved among mammalian hepadnaviruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Yeh CT,Liaw YF,Ou JHdoi
10.1128/JVI.64.12.6141-6147.1990subject
Has Abstractpub_date
1990-12-01 00:00:00pages
6141-7issue
12eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::The replication and transcription of double-stranded RNA (dsRNA) viruses occur within a polymerase complex particle in which the viral genome is enclosed throughout the entire life cycle of the virus. A single protein subunit in the polymerase complex is responsible for the template-dependent RNA polymerization activi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01044-08
更新日期:2008-09-01 00:00:00
abstract::Cells latently infected with herpes simplex virus (HSV) contain nucleosomal DNA similar to that of host cell chromatin. Recent studies have demonstrated that histones in the latency-associated transcript (LAT) promoter and intron regions contain histone modifications permissive for transcription. However, those histon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02447-06
更新日期:2007-05-01 00:00:00
abstract::While epidermal growth factor receptor (EGFR) has been shown to be important in the entry process for multiple viruses, including hepatitis C virus (HCV), the molecular mechanisms by which EGFR facilitates HCV entry are not well understood. Using the infectious cell culture HCV model (HCVcc), we demonstrate that the b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00750-12
更新日期:2012-10-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) is a recently described arterivirus responsible for disease in swine worldwide. Comparative sequence analysis of 3'-terminal structural genes of the single-stranded RNA viral genome revealed the presence of two genotypic classes of PRRSV, represented by the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.270-280.1999
更新日期:1999-01-01 00:00:00
abstract::Hepatitis B virus (HBV) is an enveloped DNA virus that presumably buds at intracellular membranes of infected cells. HBV budding involves two endocytic host proteins, the ubiquitin-interacting adaptor gamma 2-adaptin and the Nedd4 ubiquitin ligase. Here, we demonstrate that HBV release also requires the cellular machi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00479-07
更新日期:2007-09-01 00:00:00
abstract::Herpesvirus saimiri (HVS) is the prototype gamma-2 herpesvirus, which naturally infects the squirrel monkey Saimiri sciureus, causing an asymptomatic but persistent infection. The latent phase of gamma-2 herpesviruses is characterized by their ability to persist in a dividing cell population while expressing a limited...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02171-06
更新日期:2007-04-01 00:00:00
abstract::Three defense functions of immunoglobulin A (IgA), immune exclusion, intracellular neutralization, and virus excretion, were assessed in a measles virus model using polarized epithelial cells expressing the polymeric immunoglobulin receptor and monoclonal antibodies against the viral H and F envelope proteins and the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.10972-10979.2002
更新日期:2002-11-01 00:00:00
abstract::We have examined the attachment and penetration phenotypes of several glycoprotein gIII mutants of pseudorabies virus (PRV) and have identified the first one-third of gIII as a region that mediates efficient virus attachment to PK15 and Vero cells. This portion of gIII, amino acids 25 through 157 of the wild-type sequ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2646-2654.1993
更新日期:1993-05-01 00:00:00
abstract::Lentiviruses are nononcogenic retroviruses that cause persistent infections and slowly progressive diseases. Visna virus, a lentivirus of sheep, persists in cells of the macrophage lineage despite the presence of neutralizing antibodies in the animal. These antibodies are measured by prevention of virus replication in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.1.37-44.1986
更新日期:1986-07-01 00:00:00
abstract::RNA was extracted from the Burkitt lymphoma-derived cell line Raji and from Burkitt lymphoma tumor biopsies, isotope labeled in vitro by iodination with 125I, and hybridized to electrophoretically separated restriction endonuclease fragments of Epstein-Barr virus DNA on nitrocellulose membranes. The results indicated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.8-18.1979
更新日期:1979-10-01 00:00:00
abstract::Tryptic digests of four polypeptides found in Kunjin virus-infected Vero cells, NV5, NV4, V3, and NV3, were compared by peptide mapping. The polypeptides to be analyzed were labeled with radioactive methionine and separated by electrophoresis through polyacrylamide gels containing sodium dodecyl sulfate. Because infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.2.651-661.1977
更新日期:1977-11-01 00:00:00
abstract::BALB/c mice infected on the scarified cornea with herpes simplex virus type 1 strain 35 [HSV-1(35)] rarely developed ocular disease even at challenge doses as high as 10(7) PFU per eye. In contrast, HSV-1(RE) consistently induced stromal keratitis at an inoculum of 2 x 10(4) PFU. The goal of this study was to determin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2187-2192.1990
更新日期:1990-05-01 00:00:00
abstract::Members of the Geminiviridae have single-stranded DNA genomes that replicate in nuclei of infected plant cells. All geminiviruses encode a conserved protein (Rep) that catalyzes initiation of rolling-circle replication. Earlier studies showed that three conserved motifs-motifs I, II, and III-in the N termini of gemini...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02143-10
更新日期:2011-02-01 00:00:00
abstract::Leishmania RNA virus (LRV) is a double-stranded RNA virus that infects some strains of the protozoan parasite leishmania As with other totiviruses, LRV presumably expresses its polymerase by a ribosomal frameshift, resulting in a capsid-polymerase fusion protein. We have demonstrated previously that an LRV capsid-poly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10448-10455.2003
更新日期:2003-10-01 00:00:00
abstract::Arginine methylation of human immunodeficiency virus type 1 (HIV-1) Tat protein downregulates its key function in viral-gene transactivation. The fate of methylated Tat is unknown, so it is unclear whether methylated Tat is degraded or persists in the cell for additional functions. Here we show that the arginine methy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00499-09
更新日期:2009-11-01 00:00:00
abstract::CtBP has been shown to be a highly conserved corepressor of transcription. E1A and all the various transcription factors to which CtBP binds contain a conserved PLDLS CtBP-interacting domain, and EBNA3C includes a PLDLS motif (amino acids [aa] 728 to 732). Here we show that EBNA3C binds to CtBP both in vitro and in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7749-7755.2001
更新日期:2001-08-01 00:00:00
abstract::Tetherin (CD317/BST-2), an interferon-induced membrane protein, restricts the release of nascent retroviral particles from infected cell surfaces. While human immunodeficiency virus type 1 (HIV-1) encodes the accessory gene vpu to overcome the action of tetherin, the lineage of primate lentiviruses that gave rise to H...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01515-09
更新日期:2009-11-01 00:00:00
abstract::During the acute phase of infection with influenza A virus, the degree of lymphopenia correlates with severity of disease. Factors that contribute to T-cell activation during influenza virus infection may contribute to this observation. Since the immune response is initiated when dendritic cells (DC) interact with T c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5460-5469.2000
更新日期:2000-06-01 00:00:00
abstract:UNLABELLED:Enterovirus A71 (EV-A71) is a major cause of hand, foot, and mouth disease (HFMD) and is particularly prevalent in parts of Southeast Asia, affecting thousands of children and infants each year. Revealing the evolutionary and epidemiological dynamics of EV-A71 through time and space is central to understandi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00706-15
更新日期:2015-09-01 00:00:00
abstract::The Vpr accessory gene product of human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus is believed to play a role in permitting entry of the viral core into the nucleus of nondividing cells. A second role for Vpr was recently suggested by Rogel et al. (M. E. Rogel, L. I. Wu, and M. Emerman, J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6705-6711.1995
更新日期:1995-11-01 00:00:00
abstract::Nucleic acids from an unidentified virus from ringed seals (Phoca hispida) were amplified using sequence-independent PCR, subcloned, and then sequenced. The full genome of a novel RNA virus was derived, identifying the first sequence-confirmed picornavirus in a marine mammal. The phylogenetic position of the tentative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01240-07
更新日期:2008-01-01 00:00:00
abstract::The first step in the replication of the plus-stranded poliovirus RNA is the synthesis of a complementary minus strand. This process is initiated by the covalent attachment of UMP to the terminal protein VPg, yielding VPgpU and VPgpUpU. We have previously shown that these products can be made in vitro in a reaction th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10359-10370.2000
更新日期:2000-11-01 00:00:00
abstract::Yaba tumor poxvirus deoxyribonucleic acid (DNA) has a density of 1.6905 in CsCl and its T(m) value in 0.015 m citrate in saline is 82.3. The guanine plus cytosine content estimated from these properties was taken to be 32.5 +/- 0.5%, a value 2 to 3% less than for DNA from other poxviruses reported to date. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.2.114-118.1969
更新日期:1969-02-01 00:00:00
abstract::Following its entry into cells, pseudorabies virus (PRV) utilizes microtubules to deliver its nucleocapsid to the nucleus. Previous studies have shown that PRV VP1/2 is an effector of dynein-mediated capsid transport. However, the mechanism of PRV for recruiting microtubule motor proteins for successful neuroinvasion ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02052-18
更新日期:2019-03-21 00:00:00
abstract::Human cytomegalovirus (HCMV) exhibits a highly restricted host range. In this study, we sought to examine the relative significance of host and viral factors in activating early gene expression of the HCMV UL54 (DNA polymerase) promoter in murine cells. Appropriate activation of the UL54 promoter at early times is ess...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.26-35.2001
更新日期:2001-01-01 00:00:00
abstract::Sequence analysis of the short unique (Us) segment of the bovine herpesvirus 1 (BHV-1) genome predicted that the Us open reading frame (ORF) 4 encodes a protein with homology to glycoprotein G (gG) of other alpha-herpesviruses (P. Leung-Tack, J.-C. Audonnet, and M. Riviere, Virology 199:409-421, 1994). RNA analysis sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3032-3038.1996
更新日期:1996-05-01 00:00:00
abstract:UNLABELLED:Hepatitis C virus (HCV) infects 180 million people worldwide and is a leading cause of liver diseases such as fibrosis, cirrhosis, and hepatocellular carcinoma. It has been shown that HCV can spread to naive cells using two distinct entry mechanisms, "cell-free" entry of infectious extracellular virions that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03241-13
更新日期:2014-05-01 00:00:00
abstract::Progressive multifocal leukoencephalopathy (PML) is an often-fatal demyelinating disease of the central nervous system. PML results when oligodendrocytes within immunocompromised individuals are infected with the human JC virus (JCV). We have identified an oligodendrocyte precursor cell line, termed G144, that support...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00735-17
更新日期:2017-09-27 00:00:00
abstract::The nucleoprotein of the WSN strain of influenza was found to be phosphorylated in vitro. The phosphate-protein bond was stable to hot trichloroacetic acid, RNase, DNase, succinic acid, and succinic acid-hydroxylamine, but sensitive to hydrolysis by bacterial alkaline phosphatase. This suggested that the nucleoprotein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.401-405.1977
更新日期:1977-10-01 00:00:00
abstract::The human cytomegalovirus UL4 gene encodes a 48-kDa glycoprotein, expression of which is repressed at the translational level by a short upstream open reading frame (uORF2) within the UL4 transcript leader. Mutation of the uORF2 initiation codon in the viral genome eliminates ribosomal stalling at the uORF2 terminatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.7188-7192.2001
更新日期:2001-08-01 00:00:00