Identification of transcribed regions of Epstein-Barr virus DNA in Burkitt lymphoma-derived cells.

Abstract:

:RNA was extracted from the Burkitt lymphoma-derived cell line Raji and from Burkitt lymphoma tumor biopsies, isotope labeled in vitro by iodination with 125I, and hybridized to electrophoretically separated restriction endonuclease fragments of Epstein-Barr virus DNA on nitrocellulose membranes. The results indicated that only certain parts of the Epstein-Barr virus genome are represented as polyribosomal RNA in Raji cells, with a pronounced dominance of RNA sequences complementary to a 2.0 x 10(6)-dalton segment of Epstein-Barr virus DNA located close to the left end of the viral genome. A map of virus-specific polyribosomal RNA sequences was constructed, which indicated that a minimum of three regions of the Epstein-Barr virus genome are expressed in Raji cells. Total-cell RNA preparations from five Burkitt lymphoma biopsies contained RNA sequences homologous to the same regions of Epstein-Barr virus DNA as polyribosomal RNA from Raji cells, albeit at different relative proportions.

journal_name

J Virol

journal_title

Journal of virology

authors

Rymo L

doi

10.1128/JVI.32.1.8-18.1979

subject

Has Abstract

pub_date

1979-10-01 00:00:00

pages

8-18

issue

1

eissn

0022-538X

issn

1098-5514

journal_volume

32

pub_type

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