Abstract:
:The adenovirus major late promoter (MLP) is active during both the early and late phases of infection. During the early phase the activity of the MLP is similar to those of the other early viral promoters, but during the late phase the rate of transcription from the MLP becomes much greater by comparison. We report here that sequence-specific binding proteins are induced during the late phase which interact with three regions in the first intron of the MLP transcription unit from positions +37 to +68, +80 to +105, and +105 to +125 relative to the transcription initiation site. To measure the significance of these binding sites for transcription during the late phase, we constructed MLP-beta-globin fusions and substituted them for early region 3 in adenovirus recombinants. Deletion of the binding sites caused significant reductions in the rate of transcription, specifically during the late phase of infection. Deletion of all three sites reduced the rate of transcription 25- to 50-fold and the accumulation of cytoplasmic MLP-beta-globin RNA 200-fold. These results indicate that the high rate of transcription from the MLP during the late phase of infection results from the interaction of virus-induced transcription factors with three binding sites in the first intron of the major late transcription unit.
journal_name
J Viroljournal_title
Journal of virologyauthors
Leong K,Lee W,Berk AJdoi
10.1128/JVI.64.1.51-60.1990subject
Has Abstractpub_date
1990-01-01 00:00:00pages
51-60issue
1eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::Lipid biosynthesis was measured in cultured chicken embryo cells after infection with fowlpox virus. Between 24 and 72 h postinfection, fowlpox virus-infected cells incorporated less [14C]acetate and 3H2O into fatty acids and sterols than did mock-infected cells, demonstrating a virus-dependent inhibition of general l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.2.749-752.1982
更新日期:1982-08-01 00:00:00
abstract:UNLABELLED:The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an endoplasmic reticulum (ER)-resident enzyme that synthesizes phosphatidylinositol 4-phosphate (PI4P). PI4KIIIα is an essential host factor for hepatitis C virus (HCV) replication. Interaction with HCV nonstructural protein 5A (NS5A) lea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01063-14
更新日期:2014-09-01 00:00:00
abstract:UNLABELLED:Influenza virus hemagglutinin (HA) mediates virus entry by binding to cell surface receptors and fusing the viral and endosomal membranes following uptake by endocytosis. The acidic environment of endosomes triggers a large-scale conformational change in the transmembrane subunit of HA (HA2) involving a loop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00939-15
更新日期:2015-10-01 00:00:00
abstract::In vitro mapping studies of the MD145 norovirus (Caliciviridae) ORF1 polyprotein identified two stable cleavage products containing the viral RNA-dependent RNA polymerase (RdRp) domains: ProPol (a precursor comprised of both the proteinase and polymerase) and Pol (the mature polymerase). The goal of this study was to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2393-2403.2005
更新日期:2005-02-01 00:00:00
abstract::Hepatitis E virus (HEV), a positive-strand RNA virus, is an important causative agent of waterborne hepatitis. Expression of cDNA (encoding amino acids 1 to 979 of HEV nonstructural open reading frame 1) in insect cells resulted in synthesis of a 110-kDa protein (P110), a fraction of which was proteolytically processe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6249-6255.2001
更新日期:2001-07-01 00:00:00
abstract::The relatively small package capacity (less than 5 kb) of adeno-associated virus (AAV) vectors has been effectively doubled with the development of dual-vector heterodimerization approaches. However, the efficiency of such dual-vector systems is limited not only by the extent to which intermolecular recombination occu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.364-379.2005
更新日期:2005-01-01 00:00:00
abstract::We describe here the neurovirulence properties of a herpes simplex virus type 1 gamma34.5 second-site suppressor mutant. gamma34.5 mutants are nonneurovirulent in animals and fail to grow in a variety of cultured cells due to a block at the level of protein synthesis. Extragenic suppressors with restored capacity to r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5189-5196.2001
更新日期:2001-06-01 00:00:00
abstract::Membrane-containing extracts isolated from tobacco plants infected with the plus-strand RNA virus, potato virus X (PVX), supported synthesis of four major, high-molecular-weight PVX RNA products (R1 to R4). Nuclease digestion and hybridization studies indicated that R1 and R2 are a mixture of partially single-stranded...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4795-4799.1996
更新日期:1996-07-01 00:00:00
abstract::Influenza virus endonuclease activity was studied in vitro with model virion RNA (vRNA) and cRNA molecules. We show that endonuclease activity can be partially rescued by transplanting vRNA-like promoter features into the model cRNA promoter. This study defines three distinctive features within the vRNA promoter--abse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.2019-2023.2002
更新日期:2002-02-01 00:00:00
abstract::Inhibition of host-directed gene expression by the matrix (M) protein of vesicular stomatitis virus (VSV) effectively blocks host antiviral responses, promotes virus replication, and disables the host cell. However, dendritic cells (DC) have the capacity to resist these effects and remain functional during VSV infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00680-13
更新日期:2013-11-01 00:00:00
abstract::In this report, we describe the isolation, molecular genetic mapping, and phenotypic characterization of vaccinia virus mutants resistant to cytosine arabinoside (araC) and phosphonoacetic acid (PAA). At 37 degrees C, 8 microM araC was found to prevent macroscopic plaque formation by wild-type virus and to cause a 10(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4323-4336.1993
更新日期:1993-07-01 00:00:00
abstract::A system has been developed for generating chimeric yellow fever/Japanese encephalitis (YF/JE) viruses from cDNA templates encoding the structural proteins prM and E of JE virus within the backbone of a molecular clone of the YF17D strain. Chimeric viruses incorporating the proteins of two JE strains, SA14-14-2 (human...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3095-3101.1999
更新日期:1999-04-01 00:00:00
abstract::The human cytidine deaminase Apobec3F (h-A3F), a protein related to the previously recognized antiviral factor Apobec3G (h-A3G), has antiviral activity against human immunodeficiency virus type 1 (HIV-1) that is suppressed by the viral protein Vif. The mechanism of HIV-1 Vif-mediated suppression of h-A3F is not fully ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.9579-9587.2005
更新日期:2005-08-01 00:00:00
abstract::It has been suggested that vaccination prior to infection may direct the mutational evolution of human immunodeficiency virus type 1 (HIV-1) to a less fit virus, resulting in an attenuated course of disease. The present study was initiated to explore whether prior immunization might prevent the reversion of the virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01193-10
更新日期:2010-12-01 00:00:00
abstract::In the influenza H5N1 virus incident in Hong Kong in 1997, viruses that are closely related to H5N1 viruses initially isolated in a severe outbreak of avian influenza in chickens were isolated from humans, signaling the possibility of an incipient pandemic. However, it was not possible to prepare a vaccine against the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8303-8307.1999
更新日期:1999-10-01 00:00:00
abstract::BALB/c mice sensitized to herpes simplex virus type 1 (HSV-1) develop a vigorous delayed-type hypersensitivity (DTH) response upon intradermal virus antigen challenge. Although CD4(+) T cells are a key mediator of this response, neutrophils are the most abundant cells at the antigen challenge site both initially and a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8050-8057.2002
更新日期:2002-08-01 00:00:00
abstract::Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome consisting of six negative-stranded RNA segments. To rescue a recombinant THOV, the viral structural proteins were produced from expression plasmids by means of a vaccinia virus expressing the T7 RNA polymerase. Genomic virus RNAs (vRNAs) were gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9282-9286.2001
更新日期:2001-10-01 00:00:00
abstract::Nonconservative substitutions for Tyr-115 in the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) lead to enzymes displaying lower affinity for deoxynucleoside triphosphates (dNTPs) (A. M. Martín-Hernández, E. Domingo, and L. Menéndez-Arias, EMBO J. 15:4434-4442, 1996). Several mutations at th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6293-6298.1999
更新日期:1999-08-01 00:00:00
abstract::Infection of cells with poliovirus induces a massive intracellular membrane reorganization to form vesicle-like structures where viral RNA replication occurs. The mechanism of membrane remodeling remains unknown, although some observations have implicated components of the cellular secretory and/or autophagy pathways....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01820-06
更新日期:2007-01-01 00:00:00
abstract::Vaccine-elicited humoral immune responses comprise an array of antibody forms and specificities, with only a fraction contributing to protective host immunity. Elucidation of antibody effector functions responsible for protective immunity against human immunodeficiency virus type 1 (HIV-1) acquisition is a major goal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01552-17
更新日期:2018-03-14 00:00:00
abstract::Candidate foot-and-mouth disease (FMD) DNA vaccines designed to produce viral capsids lacking infectious viral nucleic acid were evaluated. Plasmid DNAs containing a portion of the FMDV genome coding for the capsid precursor protein (P1-2A) and wild-type or mutant viral proteinase 3C (plasmids P12X3C or P12X3C-mut, re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4454-4457.1998
更新日期:1998-05-01 00:00:00
abstract::Mengovirus 14S subviral protein particles generated in infected L cells and in a cell-free translation system primed with mengovirus RNA were purified by sucrose gradient centrifugation and immunoaffinity chromatography. The preparations from both sources contained essentially pure proteins epsilon, alpha, and gamma, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.275-284.1986
更新日期:1986-01-01 00:00:00
abstract:UNLABELLED:As a herpesvirus, Epstein-Barr virus (EBV) establishes a latent infection that can periodically undergo reactivation, resulting in lytic replication and the production of new infectious virus. Latent membrane protein-1 (LMP1), the principal viral oncoprotein, is a latency-associated protein implicated in reg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00711-15
更新日期:2015-08-01 00:00:00
abstract::The M2-2 protein of respiratory syncytial virus (RSV) is involved in regulation of viral RNA transcription and replication. Encoded by the next-to-last gene of RSV, the M2-2 open reading frame (ORF) overlaps with the upstream M2-1 ORF, suggesting that the production of the M2-2 protein might be tightly regulated durin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.13943-13952.2005
更新日期:2005-11-01 00:00:00
abstract::Monoclonal antibodies (MAbs) to human immunodeficiency virus type 1 were produced. Two antibodies reacted with the 17-kilodalton core protein (p17) of the virus and with its polyprotein precursor. To various degrees, each MAb neutralized infection by the cell-free virus. With a series of sequential overlapping hexapep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.267-272.1989
更新日期:1989-01-01 00:00:00
abstract::The epithelium plays a key role in the spread of Lassa virus. Transmission from rodents to humans occurs mainly via inhalation or ingestion of droplets, dust, or food contaminated with rodent urine. Here, we investigated Lassa virus infection in cultured epithelial cells and subsequent release of progeny viruses. We s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02240-09
更新日期:2010-04-01 00:00:00
abstract::Prestimulation of the TLR4 pathway with lipopolysaccharide (LPS) protects mice from lethal infection with H5N1 influenza virus. Here, we reveal that the TLR4-TRIF pathway is required for this protective effect by using mice whose TLR4-related molecules were knocked out. Microarray analysis of primary mouse lung cultur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06168-11
更新日期:2012-01-01 00:00:00
abstract::The entry of animal viruses into cells is associated with permeabilization of the infected cells to protein toxins such as alpha-sarcin (C. Fernández-Puentes and L. Carrasco, Cell 20:769-775, 1980). This phenomenon has been referred to as "the early permeabilization by animal viruses" (L. Carrasco, Virology 113:623-62...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2572-2577.1991
更新日期:1991-05-01 00:00:00
abstract::We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the G...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5300-5305.1994
更新日期:1994-08-01 00:00:00
abstract::The Epstein-Barr virus (EBV) major envelope glycoprotein gp340 is the subject of current efforts to develop an EBV subunit vaccine. The importance of gp340-specific humoral immunity has been highlighted by studies of natural infection in humans and gp340 immunization of experimental animals. The former studies have de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.1246-1251.1992
更新日期:1992-02-01 00:00:00