Abstract:
:In the influenza H5N1 virus incident in Hong Kong in 1997, viruses that are closely related to H5N1 viruses initially isolated in a severe outbreak of avian influenza in chickens were isolated from humans, signaling the possibility of an incipient pandemic. However, it was not possible to prepare a vaccine against the virus in the conventional embryonated egg system because of the lethality of the virus for chicken embryos and the high level of biosafety therefore required for vaccine production. Alternative approaches, including an avirulent H5N4 virus isolated from a migratory duck as a surrogate virus, H5N1 virus as a reassortant with avian virus H3N1 and an avirulent recombinant H5N1 virus generated by reverse genetics, have been explored. All vaccines were formalin inactivated. Intraperitoneal immunization of mice with each of vaccines elicited the production of hemagglutination-inhibiting and virus-neutralizing antibodies, while intranasal vaccination without adjuvant induced both mucosal and systemic antibody responses that protected the mice from lethal H5N1 virus challenge. Surveillance of birds and animals, particularly aquatic birds, for viruses to provide vaccine strains, especially surrogate viruses, for a future pandemic is stressed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Takada A,Kuboki N,Okazaki K,Ninomiya A,Tanaka H,Ozaki H,Itamura S,Nishimura H,Enami M,Tashiro M,Shortridge KF,Kida Hdoi
10.1128/JVI.73.10.8303-8307.1999subject
Has Abstractpub_date
1999-10-01 00:00:00pages
8303-7issue
10eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::We have been investigating the first viral genes to be transcribed in Spodoptera frugiperda cells after the infection with Autographa california nuclear polyhedrosis virus in order to identify regulatory proteins required for the activation of early and/or late gene transcription. By screening a cDNA library from poly...
journal_title:Journal of virology
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doi:10.1128/JVI.67.2.753-758.1993
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.6.1164-1167.1967
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.10003-10012.2005
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.115-123.1978
更新日期:1978-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3557-3564.2005
更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.1.20-25.1984
更新日期:1984-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2629-2635.1988
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.5814-5819.1997
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00