Abstract:
:Viral protein R (Vpr) of human immunodeficiency virus type 1 has potent karyophilic properties, but details of the mechanism by which it enters the nucleus remain to be clarified. We reported previously that two regions, located between residues 17 and 34 (alphaH1) and between residues 46 and 74 (alphaH2), are indispensable for the nuclear localization of Vpr. Here, we reveal that a chimeric protein composed of the nuclear localization signal of Vpr, glutathione S-transferase, and green fluorescent protein was localized at the nuclear envelope and then entered the nucleus upon addition of importin-alpha. An in vitro transport assay using a series of derivatives of importin-alpha demonstrated that the carboxyl terminus was required for this nuclear import process. We also showed that Vpr interacts with importin-alpha through alphaH1 and alphaH2; only the interaction via alphaH1 is indispensable for the nuclear entry of Vpr. These observations indicate that importin-alpha functions as a mediator for the nuclear entry of Vpr.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kamata M,Nitahara-Kasahara Y,Miyamoto Y,Yoneda Y,Aida Ydoi
10.1128/JVI.79.6.3557-3564.2005subject
Has Abstractpub_date
2005-03-01 00:00:00pages
3557-64issue
6eissn
0022-538Xissn
1098-5514pii
79/6/3557journal_volume
79pub_type
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