Abstract:
:The coding properties of RNA transcribed in vitro by purified vaccinia cores have been investigated using Krebs ascites tumor cells, L cells, and reticulocyte lysates. Six to 10 proteins synthesized in vitro are separated on polyacrylamide gels by electrophoresis in the presence of sodium dodecyl sulfate. Their molecular weights vary from 10,000 to 44,000. The electrophoretic behavior of these proteins is similar to that of early proteins isolated from infected L cells. The tryptic peptide analysis of one of these proteins indicates similarity in amino acid sequences. These results show fidelity of both in vitro transcription and molecular weight above 44,000 are synthesized in vitro does not seem due to a competition between 12S mRNA synthesized in excess and RNA of a higher sedimentation coefficient present in a lower amount.
journal_name
J Viroljournal_title
Journal of virologyauthors
Jaureguiberry G,Ben-Hamida F,Chapeville F,Beaud Gdoi
10.1128/JVI.15.6.1467-1474.1975subject
Has Abstractpub_date
1975-06-01 00:00:00pages
1467-74issue
6eissn
0022-538Xissn
1098-5514journal_volume
15pub_type
杂志文章abstract::Human cytomegalovirus (HCMV) pathogenesis is dependent on the hematogenous spread of the virus to host tissue. While data suggest that infected monocytes are required for viral dissemination from the blood to the host organs, infected endothelial cells are also thought to contribute to this key step in viral pathogene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01016-06
更新日期:2006-12-01 00:00:00
abstract:UNLABELLED:Influenza A virus (IAV) entry is a multistep process that requires the interaction of the virus with numerous host factors. In this study, we demonstrate that prolidase (PEPD) is a cellular factor required by IAV for successful entry into target cells. PEPD was selected as a candidate during an entry screen ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00800-14
更新日期:2014-10-01 00:00:00
abstract::Genetic studies with immunocompetent mice show the importance of both T cells and gamma interferon (IFN-γ) for survival of a measles virus (MV) challenge; however, the direct role of T cells and IFN-γ within the MV-infected brain has not been addressed. Organotypic brain explants represent a successful ex vivo system ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01496-10
更新日期:2011-04-01 00:00:00
abstract::Polyclonal and monoclonal antibodies to individual herpes simplex virus (HSV) glycoproteins were tested for ability to inhibit adsorption of radiolabeled HSV type 1 (HSV-1) strain HFEMsyn [HSV-1(HFEM)syn] to HEp-2 cell monolayers. Polyclonal rabbit antibodies specific for glycoprotein D (gD) or gC and three monoclonal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.2.475-482.1985
更新日期:1985-08-01 00:00:00
abstract::Human cytomegalovirus encodes four unique short (US) region proteins, each of which is independently sufficient for causing the down-regulation of major histocompatibility complex (MHC) class I molecules on the cell surface. This down-regulation enables infected cells to evade recognition by cytotoxic T lymphocytes (C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02397-05
更新日期:2006-06-01 00:00:00
abstract::Chikungunya virus (CHIKV) is a mosquito-borne alphavirus responsible for chikungunya fever. Nonstructural protein 2 (nsP2), a multifunctional protein essential for viral replication, has an N-terminal helicase region (nsP2h), which as both nucleotide triphosphatase and RNA triphosphatase activities, as well as a C-ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01470-20
更新日期:2020-12-16 00:00:00
abstract::In previous studies, we demonstrated that single-chain variable fragments (scFvs) from anti-human immunodeficiency virus (HIV) Env monoclonal antibodies act as entry inhibitors when tethered to the surface of target cells by a glycosyl-phosphatidylinositol (GPI) anchor. Interestingly, even if a virus escapes inhibitio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02080-17
更新日期:2018-03-14 00:00:00
abstract::Nonstructural protein 5B (NS5B) of bovine viral diarrhea virus (BVDV) contains sequence motifs that are predictive of an RNA-dependent RNA polymerase activity. We describe the expression and purification of the BVDV NS5B protein derived from an infectious cDNA clone of BVDV (NADL strain). BVDV NS5B protein was active ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9365-9369.1998
更新日期:1998-11-01 00:00:00
abstract::Previously we have described a stepwise, energy-dependent pathway for human immunodeficiency virus type 1 (HIV-1) capsid assembly in a cell-free system. In this pathway, Gag polypeptides utilize the cellular factor HP68 and assemble into immature capsids by way of assembly intermediates that have defined biochemical c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1645-1656.2004
更新日期:2004-02-01 00:00:00
abstract:UNLABELLED:Based on an explant reactivation model, it has been proposed that CD8(+) T cells maintain latency in trigeminal ganglia (TG) of mice latently infected with herpes simplex virus 1 (HSV-1) [T. Liu, K. M. Khanna, X. Chen, D. J. Fink, and R. L. Hendricks, J Exp Med 191:1459-1466, 2000, doi:10.1084/jem.191.9.1459...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00255-16
更新日期:2016-04-29 00:00:00
abstract::The human T-cell leukemia virus type 1 (HTLV-1) transforming protein, Tax, is a potent transactivator of both viral and cellular gene expression. The ability of Tax to transform cells is believed to depend on its transactivation of cellular-growth-regulatory genes. Expression of proliferating cell nuclear antigen (PCN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1181-1190.1997
更新日期:1997-02-01 00:00:00
abstract::A number of naturally occurring hepatitis B virus mutants that cannot synthesize the virus precore protein have been identified. Such mutants have been associated with more severe forms of hepatitis, including fulminant hepatitis. The most common mutation observed is a substitution of G to A in the distal precore gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5682-5684.1992
更新日期:1992-09-01 00:00:00
abstract::Glycoprotein gIII of pseudorabies virus is a major antigen found in the envelopes of virus particles as well as in and on the surfaces of infected cells. It is not an essential gene product for virus growth in tissue culture. In this report, we provide evidence that, although it is not essential, the gIII protein is r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2512-2515.1988
更新日期:1988-07-01 00:00:00
abstract::Caprine arthritis encephalitis virus (CAEV) is a lentivirus closely related to visna virus and more distantly to other lentiviruses, such as human immunodeficiency virus. The genomes of visna virus and CAEV contain a tat gene encoding a protein able to weakly transactivate its own long terminal repeat, suggesting that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5445-5454.1995
更新日期:1995-09-01 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68) is a gammaherpesvirus that was first isolated from murid rodents. MHV68 establishes a latent infection in the spleen and other lymphoid organs. Several gammaherpesviruses, including herpesvirus saimiri, human herpesvirus 8, and MHV68, encode proteins with extensive homology to the D-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7016-7023.2000
更新日期:2000-08-01 00:00:00
abstract:UNLABELLED:Hepatitis B, which caused by hepatitis B virus (HBV) infection, remains a major health threat worldwide. Hepatic injury and regeneration from chronic inflammation are the main driving factors of liver fibrosis and cirrhosis in chronic hepatitis B. Proinflammatory tumor necrosis factor alpha (TNF-α) has been ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03106-14
更新日期:2015-02-01 00:00:00
abstract::We have recently described an intracellular protein, p21, in nonproducer cells transformed by either the Kirsten (Ki-MSV) or Harvey (Ha-MSV) strain of murine sarcoma virus (Shih et al., Virology, in press). The p21 is phosphorylated and has been shown to be coded for by either Ki-MSV or Ha-MSV. In this report, we comp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.2.546-546.1979
更新日期:1979-08-01 00:00:00
abstract::The unique short (Us) region of the pseudorabies virus (PRV) genome, which displays high transcriptional activity during the late phase of infection and has been found to code for glycoproteins, is partially deleted in the genomes of three vaccine strains (A57, Norden, and NIA-4). This deletion is located in the SalI ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.307-311.1985
更新日期:1985-10-01 00:00:00
abstract::We report that adaptation to infect the guinea pig did not modify the capacity of foot-and-mouth disease virus (FMDV) to kill suckling mice and to cause an acute and transmissible disease in the pig, an important natural host for this pathogen. Adaptive amino acid replacements (I(248)-->T in 2C, Q(44)-->R in 3A, and L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00340-07
更新日期:2007-08-01 00:00:00
abstract::Cytoplasmic virus-specific RNA and polyribosomes from a chronically infected feline thymus tumor cell line, F-422, were analyzed by using in vitro-synthesized feline leukemia virus (Rickard strain) (R-FeLV) complementary DNA (cDNA) probe. By hybridization kinetics analysis, cytoplasmic, polyribosomat, and nuclear RNAs...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.3.750-763.1978
更新日期:1978-03-01 00:00:00
abstract::Using isogenic recombinant murine coronaviruses expressing wild-type murine hepatitis virus strain 4 (MHV-4) or MHV-A59 spike glycoproteins or chimeric MHV-4/MHV-A59 spike glycoproteins, we have demonstrated the biological functionality of the N-terminus of the spike, encompassing the receptor binding domain (RBD). We...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.841-850.2003
更新日期:2003-01-01 00:00:00
abstract:UNLABELLED:We have shown that glycoprotein K (gK) and its interacting partner, the UL20 protein, play crucial roles in virion envelopment. Specifically, virions lacking either gK or UL20 fail to acquire an envelope, thus causing accumulation of capsids in the cytoplasm of infected cells. The herpes simplex virus 1 (HSV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00278-14
更新日期:2014-06-01 00:00:00
abstract::Three serial passage series of simian virus 40 (SV40) in CV-1 cells were initiated by infection directly from the same wild-type plaque isolate, three series were initiated by infection with another plaque isolate, and two series were initiated with each of two other plaque isolates. Aberrant SV40 genomes were not det...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.2.730-733.1982
更新日期:1982-05-01 00:00:00
abstract::Recruitment and extravasation of T cells through the blood-brain barrier are favored by adhesion molecule-mediated interactions of circulating T cells with endothelial cells. Since a common pathological finding in human T-cell leukemia virus type 1 (HTLV-1)-associated diseases is the infiltration of HTLV-1-infected T ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8522-8530.1997
更新日期:1997-11-01 00:00:00
abstract::The Kaposi's sarcoma-associated herpesvirus nuclear egress complex is composed of two proteins, ORF67 and ORF69. In this study, we have recapitulated the KSHV complex by coexpression of these two proteins in insect cells using expression from recombinant baculoviruses. The proteins form a complex at the nuclear membra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05988-11
更新日期:2012-01-01 00:00:00
abstract:UNLABELLED:Lethal mutagenesis is a broad-spectrum antiviral strategy that exploits the high mutation rate and low mutational tolerance of many RNA viruses. This approach uses mutagenic drugs to increase viral mutation rates and burden viral populations with mutations that reduce the number of infectious progeny. We inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03483-14
更新日期:2015-04-01 00:00:00
abstract::We have been studying defective interfering (DI) genomes of the RNA enveloped virus Sindbis virus. Deletion mapping of a DI cDNA demonstrated that only sequences at the 3' and 5' termini of the genome are required for the DI RNA to be biologically active. We constructed a series of cDNAs that transcribe DI RNAs differ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.1.47-53.1988
更新日期:1988-01-01 00:00:00
abstract::We demonstrate that the S4 genome segment of baboon reovirus (BRV) contains two sequential partially overlapping open reading frames (ORFs), both of which are functional in vitro and in virus-infected cells. The 15-kDa gene product (p15) of the 5"-proximal ORF induces efficient cell-cell fusion when expressed by itsel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.5.2131-2140.2002
更新日期:2002-03-01 00:00:00
abstract::Little is known about the mechanisms used by enveloped viruses to separate themselves from the cell surface at the final step of budding. However, small sequences in the Gag proteins of several retroviruses (L domains) have been implicated in this process. A sequence has been identified in the M proteins of rhabdoviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3359-3365.1999
更新日期:1999-04-01 00:00:00
abstract::The catalytic core domain (CCD) of human immunodeficiency virus type 1 (HIV-1) integrase (IN) harbors the enzyme active site and binds viral and chromosomal DNA during integration. Thirty-five CCD mutant viruses were constructed, paying particular attention to conserved residues in the Phe(139)-Gln(146) flexible loop ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2493-2505.2005
更新日期:2005-02-01 00:00:00