Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase.

abstract：:Temperature-sensitive (ts) mutants of the T4 phage rII gene were islated and used in temperature shift experiments that revelaed two different expressions for the normal rII (rII+) gene function in vivo: (i) an early expression (0 to 12 min postinfection at 30 C) that prevents restriction of T4 growth in Escherichia c...

journal_title：Journal of virology

authors： Heere LJ,Karam JD

更新日期：1975-10-01 00:00:00

abstract：:Cell-to-cell spread of tobacco mosaic virus is facilitated by the virus-encoded 30-kDa movement protein (MP). This process involves interaction of viral proteins with host components, including the cytoskeleton and the endoplasmic reticulum (ER). During virus infection, high-molecular-weight forms of MP were detected ...

journal_title：Journal of virology

authors： Reichel C,Beachy RN

更新日期：2000-04-01 00:00:00

abstract：:Viruses cause about 15% of the cancers that are still the leading causes of human mortality. The discovery of viral oncogenes has enhanced our understanding of viral oncogenesis. However, the underlying molecular mechanisms of virus-induced cancers are complex and require further investigation. The present study has a...

journal_title：Journal of virology

authors： Xu S,Xue C,Li J,Bi Y,Cao Y

更新日期：2011-01-01 00:00:00

abstract：:Clinical deterioration in human immunodeficiency virus type 1 (HIV-1) disease is associated with an increased viral burden in the peripheral blood and a loss of circulating CD4+ T cells. HIV-1 isolates obtained prior to this stage of disease often have a "slow-low," non-syncytium-inducing (NSI) phenotype, whereas thos...

journal_title：Journal of virology

authors： Kaneshima H,Su L,Bonyhadi ML,Connor RI,Ho DD,McCune JM

更新日期：1994-12-01 00:00:00

abstract：:Recently developed antiviral strategies based upon RNA interference (RNAi), which harnesses an innate cellular system for the targeted down-regulation of gene expression, appear highly promising and offer alternative approaches to conventional highly active antiretroviral therapy or efforts to develop an AIDS vaccine....

journal_title：Journal of virology

authors： Leonard JN,Schaffer DV

更新日期：2005-02-01 00:00:00

abstract：:The genetic evolution of human immunodeficiency virus type 1 (HIV-1) in the brain is distinct from that in lymphoid tissues, indicating tissue-specific compartmentalization of the virus. Few primary HIV-1 envelope glycoproteins (Envs) from uncultured brain tissues have been biologically well characterized. In this stu...

journal_title：Journal of virology

authors： Ohagen A,Devitt A,Kunstman KJ,Gorry PR,Rose PP,Korber B,Taylor J,Levy R,Murphy RL,Wolinsky SM,Gabuzda D

更新日期：2003-11-01 00:00:00

abstract：:We investigated the protective role of antibodies in vaginal secretions of mice that were immune to vaginal challenge with herpes simplex virus type 2 (HSV-2). Unfractionated vaginal immunoglobulins from immune and nonimmune mice and affinity-purified immunoglobulin G (IgG) and secretory IgA (S-IgA) from immune secret...

journal_title：Journal of virology

authors： Parr EL,Parr MB

更新日期：1997-11-01 00:00:00

abstract：:Recently it has been shown that kaposica, an immune evasion protein of Kaposi's sarcoma-associated herpesvirus, inactivates complement by acting on C3-convertases by accelerating their decay as well as by acting as a cofactor in factor I-mediated inactivation of their subunits C3b and C4b. Here, we have mapped the fun...

journal_title：Journal of virology

authors： Mullick J,Singh AK,Panse Y,Yadav V,Bernet J,Sahu A

更新日期：2005-05-01 00:00:00

abstract：:The biological activity encoded in the putative protease gene (pro) of human T-cell leukemia virus type I was investigated by using a vaccinia virus expression vector. The 53-kilodalton gag precursor polyprotein was processed into the mature p19, p24, and p15 gag proteins when the gag and protease-coding sequence was ...

journal_title：Journal of virology

authors： Nam SH,Kidokoro M,Shida H,Hatanaka M

更新日期：1988-10-01 00:00:00

abstract：:Most of the viral DNA extracted from simian virus 40 (SV40)-infected African green monkey kidney cells consists of circular molecules about 5.3 kilobases in contour length. However, about 1% of the viral DNA was found to occur as closed circular dimers that appeared to be formed, preferentially, late in infection. The...

journal_title：Journal of virology

authors： Goff SP,Berg P

更新日期：1977-10-01 00:00:00

abstract：:Coronavirus nonstructural protein 8 (nsp8) has been suggested to have diverse activities, including noncanonical template-dependent polymerase activities. Here, we characterized a recombinant form of the human coronavirus 229E (HCoV-229E) nsp8 and found that the protein has metal ion-dependent RNA 3'-terminal adenylyl...

journal_title：Journal of virology

authors： Tvarogová J,Madhugiri R,Bylapudi G,Ferguson LJ,Karl N,Ziebuhr J

更新日期：2019-05-29 00:00:00

abstract：:A general theme that has emerged from studies of DNA tumor viruses is that otherwise unrelated oncoproteins encoded by these viruses often target the same important cellular factors. Major oncogenic determinants for human adenovirus type 9 (Ad9) and high-risk human papillomaviruses (HPV) are the E4-ORF1 and E6 oncopro...

journal_title：Journal of virology

authors： Lee SS,Glaunsinger B,Mantovani F,Banks L,Javier RT

更新日期：2000-10-01 00:00:00

abstract：:Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after treatment, antigenic markers and DNA polymerase activities specific to particles of each of the two types were assayed in th...

journal_title：Journal of virology

authors： Kuff EL,Lueders KK,Orenstein JM,Wilson SH

更新日期：1976-08-01 00:00:00

abstract：:We developed antigen microarrays to profile the breadth, strength, and kinetics of epitope-specific antiviral antibody responses in vaccine trials with a simian-human immunodeficiency virus (SHIV) model for human immunodeficiency virus (HIV) infection. These arrays contained 430 distinct proteins and overlapping pepti...

journal_title：Journal of virology

authors： Neuman de Vegvar HE,Amara RR,Steinman L,Utz PJ,Robinson HL,Robinson WH

更新日期：2003-10-01 00:00:00

abstract：:Polyoma virus complementary RNA, synthesized in vitro by using highly purified Escherichia coli RNA polymerase and nondefective form I polyoma DNA, was translated in a wheat germ cell-free system. Polypeptides were synthesized that comigrated on sodium dodecyl sulfate-polyacrylamide gels with the polyoma capsid protei...

journal_title：Journal of virology

authors： Mangel WF,Hewick RM,Bayley ST,Wheeler T,Harvey R,Waterfield MD,Smith AE

更新日期：1978-02-01 00:00:00

abstract：:Utilization of internal ribosome entry segment (IRES) structures in the 5' noncoding region (5'NCR) of picornavirus RNAs for initiation of translation requires a number of host cell factors whose distribution may vary in different cells and whose requirement may vary for different picornaviruses. We have examined the ...

journal_title：Journal of virology

authors： Graff J,Cha J,Blyn LB,Ehrenfeld E

更新日期：1998-12-01 00:00:00

abstract：:Closed circular unintegrated DNA of the SEATO strain of gibbon ape leukemia virus (GaLV-S) was isolated from canine thymus fibroblasts after cocultivation with chronically infected bat lung fibroblasts. Restriction endonuclease HindIII cleaves GaLV-S DNA once, thus allowing isolation and cloning of HindIII-digested un...

journal_title：Journal of virology

authors： Gelmann EP,Trainor CD,Wong-Staal F,Reitz MS

更新日期：1982-10-01 00:00:00

abstract：:CCCTC-binding factor (CTCF) has been implicated in various aspects of viral and host chromatin organization and transcriptional control. We showed previously that CTCF binds to a cluster of three sites in the first intron of the Kaposi's sarcoma-associated herpesvirus (KSHV) multicistronic latency-associated transcrip...

journal_title：Journal of virology

authors： Kang H,Cho H,Sung GH,Lieberman PM

更新日期：2013-02-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) infects alveolar macrophages (AMϕ), causing dysregulated alpha interferon (IFN-α) and tumor necrosis factor alpha (TNF-α) production through a mechanism(s) yet to be resolved. Here, we show that AMϕ infected with PRRSV secreted a reduced quantity of IFN-α fol...

journal_title：Journal of virology

authors： Chen WY,Schniztlein WM,Calzada-Nova G,Zuckermann FA

更新日期：2018-01-02 00:00:00

abstract：:Nonhuman primates are widely used to study correlates of protective immunity in AIDS research. Successful cellular immune responses have been difficult to identify because heterogeneity within macaque major histocompatibility complex (MHC) genes results in quantitative and qualitative differences in immune responses. ...

journal_title：Journal of virology

authors： Wiseman RW,Wojcechowskyj JA,Greene JM,Blasky AJ,Gopon T,Soma T,Friedrich TC,O'Connor SL,O'Connor DH

更新日期：2007-01-01 00:00:00

abstract：:Chikungunya virus (CHIKV) is a mosquito-borne alphavirus responsible for chikungunya fever. Nonstructural protein 2 (nsP2), a multifunctional protein essential for viral replication, has an N-terminal helicase region (nsP2h), which as both nucleotide triphosphatase and RNA triphosphatase activities, as well as a C-ter...

journal_title：Journal of virology

authors： Law YS,Wang S,Tan YB,Shih O,Utt A,Goh WY,Lian BJ,Chen MW,Jeng US,Merits A,Luo D

更新日期：2020-12-16 00:00:00

abstract：:Enviroxime is an antienterovirus compound that targets viral protein 3A and/or 3AB and suppresses a step in enterovirus replication by unknown mechanism. To date, four antienterovirus compounds, i.e., GW5074, Flt3 inhibitor II, TTP-8307, and AN-12-H5, are known to have similar mutations in the 3A protein-encoding regi...

journal_title：Journal of virology

authors： Arita M,Kojima H,Nagano T,Okabe T,Wakita T,Shimizu H

更新日期：2011-03-01 00:00:00

abstract：:Treatment with sodium dodecyl sulfate (SDS) converted the vaccinia virus strain IHD-J into particles of two types: (i) ghosts which possessed a thin-membrane vesicle derived from basement part of the virus membrane with attached lateral bodies and a membranous structure derived from the core wall and (ii) aggregates o...

journal_title：Journal of virology

authors： Ichihashi Y,Oie M,Tsuruhara T

更新日期：1984-06-01 00:00:00

abstract：UNLABELLED:Pattern recognition receptors (PRR) sense certain molecular patterns uniquely expressed by pathogens. Retinoic-acid-inducible gene I (RIG-I) is a cytosolic PRR that senses viral nucleic acids and induces innate immune activation and secretion of type I interferons (IFNs). Here, using influenza vaccine antige...

journal_title：Journal of virology

authors： Kulkarni RR,Rasheed MA,Bhaumik SK,Ranjan P,Cao W,Davis C,Marisetti K,Thomas S,Gangappa S,Sambhara S,Murali-Krishna K

更新日期：2014-12-01 00:00:00

abstract：:Following intranasal administration, the severe acute respiratory syndrome (SARS) coronavirus replicated to high titers in the respiratory tracts of BALB/c mice. Peak replication was seen in the absence of disease on day 1 or 2, depending on the dose administered, and the virus was cleared within a week. Viral antigen...

journal_title：Journal of virology

authors： Subbarao K,McAuliffe J,Vogel L,Fahle G,Fischer S,Tatti K,Packard M,Shieh WJ,Zaki S,Murphy B

更新日期：2004-04-01 00:00:00

abstract：:The L1 and L2 capsid genes of human papillomavirus type 31 (HPV-31) are expressed upon keratinocyte differentiation from a promoter located in the E7 open reading frame (ORF) of the early region. Late transcripts must therefore pass through and ignore the early polyadenylation sequences to use the downstream late AAUA...

journal_title：Journal of virology

authors： Terhune SS,Hubert WG,Thomas JT,Laimins LA

更新日期：2001-09-01 00:00:00

abstract：:HLA-B*57 is strongly associated with immune control of HIV and delayed AIDS progression. The closely related, but less protective, HLA-B*58:01 presents similar epitopes, but HLA-B*58:01(+) individuals do not generate CD8(+) T cells targeting the KF11-Gag epitope, which has been linked to low viremia. Here we show that...

journal_title：Journal of virology

authors： Kløverpris HN,Stryhn A,Harndahl M,Payne R,Towers GJ,Chen F,Riddell L,Walker BD,Ndung'u T,Leslie A,Buus S,Goulder P

更新日期：2013-10-01 00:00:00

abstract：:To assess the RNA helicase activity of hepatitis C virus (HCV) nonstructural protein 3 (NS3), a polypeptide encompassing amino acids 1175 to 1657, which cover only the putative helicase domain, was expressed in Escherichia coli by a pET expression vector. The protein was purified to near homogeneity and assayed for RN...

journal_title：Journal of virology

authors： Tai CL,Chi WK,Chen DS,Hwang LH

更新日期：1996-12-01 00:00:00

abstract：UNLABELLED:The role of NF-κB in influenza A virus (IAV) infection does not reveal a coherent picture, as pro- and also antiviral functions of this transcription factor have been described. To address this issue, we used clustered regularly interspaced short palindromic repeat with Cas9 (CRISPR-Cas9)-mediated genome eng...

journal_title：Journal of virology

authors： Dam S,Kracht M,Pleschka S,Schmitz ML

更新日期：2016-08-12 00:00:00

abstract：:A panel of murine monoclonal antibodies (MAbs) to the human immunodeficiency virus type 1 trans-activator tat protein were characterized. The anti-tat MAbs were mapped to the different domains of the tat protein by Western blot (immunoblot) and Pepscan analyses. One-half of the MAbs tested mapped to the amino-terminal...

journal_title：Journal of virology

authors： Brake DA,Goudsmit J,Krone WJ,Schammel P,Appleby N,Meloen RH,Debouck C

更新日期：1990-02-01 00:00:00