Abstract:
:Most of the viral DNA extracted from simian virus 40 (SV40)-infected African green monkey kidney cells consists of circular molecules about 5.3 kilobases in contour length. However, about 1% of the viral DNA was found to occur as closed circular dimers that appeared to be formed, preferentially, late in infection. The monomeric units of dimers were organized in a head-to-tail, tandem arrangement; moreover, the monomeric units were not defective; i.e., they lacked deletions or other rearrangements. After infections with dimer DNA, nondefective monomers were formed. These findings suggest that dimers are not intermediates in the production of defective SV40 genomes. The majority of the dimers formed in mixed infections with two mutants were homodimers, but about 5% of the circular dimers were heterodimers and must have arisen by intermolecular recombination.
journal_name
J Viroljournal_title
Journal of virologyauthors
Goff SP,Berg Pdoi
10.1128/JVI.24.1.295-302.1977subject
Has Abstractpub_date
1977-10-01 00:00:00pages
295-302issue
1eissn
0022-538Xissn
1098-5514journal_volume
24pub_type
杂志文章abstract::Hemagglutinin from influenza A/PR8 virus was purified after treatment of the virus with sodium deoxycholate followed by extraction with tri-n-butyl phosphate. This fully disrupted the virus while preserving hemagglutinating activity. The hemagglutinin was obtained in the form of small aggregates that could be separate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.457-467.1978
更新日期:1978-05-01 00:00:00
abstract::Human herpesvirus 8 (HHV-8) viral interleukin-6 (vIL-6) is a cytokine that is poorly secreted and localized largely to the endoplasmic reticulum (ER). It has been implicated, along with other HHV-8 proinflammatory and/or angiogenic viral proteins, in HHV-8-associated Kaposi's sarcoma, primary effusion lymphoma (PEL), ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00909-20
更新日期:2020-09-15 00:00:00
abstract::Newcastle disease virus (NDV) edits its P-gene mRNA by inserting a nontemplated G residue(s) at a conserved editing site (3'-UUUUUCCC-template strand). In the wild-type virus, three amino-coterminal P-gene-derived proteins, P, V, and W, are produced at frequencies of approximately 68, 29, and 2%, respectively. By appl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.420-428.2001
更新日期:2001-01-01 00:00:00
abstract::The small RNA segment (S segment) of Uukuniemi (UUK) virus encodes two proteins, the nucleocapsid protein (N) and a nonstructural protein (NSs), by an ambisense strategy. The function of NSs has not been elucidated for any of the bunyaviruses expressing this protein. We have now expressed the N and NSs proteins in Sf9...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4233-4241.1992
更新日期:1992-07-01 00:00:00
abstract::CD4C/HIV(nef) transgenic (Tg) mice express Nef in CD4+ T cells and in the cells of the macrophage/monocyte/dendritic lineage, and they develop an AIDS-like disease similar to human AIDS. In these mice, Nef is constitutively expressed throughout life. To rule out the contribution of any developmental defects caused by ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01466-09
更新日期:2009-11-01 00:00:00
abstract:UNLABELLED:Influenza A virus (IAV) entry is a multistep process that requires the interaction of the virus with numerous host factors. In this study, we demonstrate that prolidase (PEPD) is a cellular factor required by IAV for successful entry into target cells. PEPD was selected as a candidate during an entry screen ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00800-14
更新日期:2014-10-01 00:00:00
abstract::Alpha/beta interferons (IFN-alpha/beta) are key mediators of innate immunity and important modulators of adaptive immunity. The mechanisms by which IFN-alpha/beta are induced are becoming increasingly well understood. Recent studies showed that Toll-like receptors 7 and 8 expressed by plasmacytoid dendritic cells (pDC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.16.10376-10385.2005
更新日期:2005-08-01 00:00:00
abstract::Infectious bursal disease virus (IBDV), a double-stranded RNA (dsRNA) virus belonging to the Birnaviridae family, is an economically important avian pathogen. The IBDV capsid is based on a single-shelled T=13 lattice, and the only structural subunits are VP2 trimers. During capsid assembly, VP2 is synthesized as a pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00077-07
更新日期:2007-07-01 00:00:00
abstract::Among the numerous immunological abnormalities observed in chronically human immunodeficiency virus (HIV)-infected individuals, perturbations in memory CD4 T cells are thought to contribute specifically to disease pathogenesis. Among these, functional imbalances in the frequencies of T regulatory cells (Tregs) and int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02163-18
更新日期:2019-04-17 00:00:00
abstract::Several C/EBP binding sites within the Rous sarcoma virus (RSV) long terminal repeat (LTR) and gag enhancers were mutated, and the effect of these mutations on viral gene expression was assessed. Minimal site-specific mutations in each of three adjacent C/EBP binding sites in the LTR reduced steady-state viral RNA lev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2862-2870.1993
更新日期:1993-05-01 00:00:00
abstract::We previously reported that herpes simplex virus type 1 (HSV-1) can activate the stress-activated protein kinases (SAPKs) p38 and JNK. In the present study, we undertook a comprehensive and comparative analysis of the requirements for viral protein synthesis in the activation of JNK and p38. Infection with the UL36 mu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8348-8360.2005
更新日期:2005-07-01 00:00:00
abstract::Latent HIV proviruses are silenced as the result of deacetylation and methylation of histones located at the viral long terminal repeat (LTR). Inhibition of histone deacetylases (HDACs) leads to the reemergence of HIV-1 from latency, but the contribution of histone lysine methyltransferases (HKMTs) to maintaining HIV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00836-11
更新日期:2011-09-01 00:00:00
abstract::The mammalian tripartite motif protein, TRIM5α, recognizes retroviral capsids entering the cytoplasm and blocks virus infection. Depending on the particular TRIM5α protein and retrovirus, complete disruption of the TRIM5α RING domain decreases virus-restricting activity to various degrees. TRIM5α exhibits RING domain-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00341-11
更新日期:2011-08-01 00:00:00
abstract::Attempts to elicit broadly neutralizing antibody responses by human immunodeficiency virus type 1 (HIV-1) vaccine antigens have been met with limited success. To better understand the requirements for cross-neutralization of HIV-1, we have characterized the neutralizing antibody specificities present in the sera of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02749-06
更新日期:2007-06-01 00:00:00
abstract::We isolated revertants of a polyomavirus whose origin of DNA replication contains a point mutation in the palindrome to which large T antigen binds. Four independent second-site revertants contain an Asp-286----Asn-286 substitution in large T antigen. This mutant large T antigen activates replication of DNAs containin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.242-249.1989
更新日期:1989-01-01 00:00:00
abstract::Recognition of viruses by germ line-encoded pattern recognition receptors of the innate immune system is essential for rapid production of type I interferon (IFN) and early antiviral defense. We investigated the mechanisms of viral recognition governing production of type I IFN during herpes simplex virus (HSV) infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01167-07
更新日期:2007-12-01 00:00:00
abstract::Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of the AAV nonstructural protein Rep78/68 to the Rep binding site (RBS) on AAVS1 is thought to be an essential step, the mechanism of the site-specific integration, particularly, how the site of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02014-06
更新日期:2007-02-01 00:00:00
abstract::We have investigated the species-specific replication of polyomavirus DNA in the cell-free system that was established previously (Y. Murakami, T. Eki, M. Yamada, C. Prives, and J. Hurwitz, Proc. Natl. Acad. Sci. USA 83:6347-6351, 1986). Extracts from various species of cells supported polyomavirus DNA replication in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4874-4881.1991
更新日期:1991-09-01 00:00:00
abstract::Measles virus (MeV) is an enveloped RNA virus bearing two envelope glycoproteins, the hemagglutinin (H) and fusion (F) proteins. Upon receptor binding, the H protein triggers conformational changes of the F protein, causing membrane fusion and subsequent virus entry. MeV may persist in the brain, infecting neurons and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01727-19
更新日期:2020-01-06 00:00:00
abstract::The matrix (M) protein of vesicular stomatitis virus (VSV) is a potent inhibitor in vivo of transcription by all three host RNA polymerases (RNAP). In the case of host RNA polymerase II (RNAPII), the inhibition is due to lack of activity of the TATA-binding protein (TBP), which is a subunit of the basal transcription ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4453-4458.2001
更新日期:2001-05-01 00:00:00
abstract::Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00046-06
更新日期:2006-06-01 00:00:00
abstract::The transmission bottleneck is defined as the number of viral particles that transmit from one host to establish an infection in another. Genome sequence data have been used to evaluate the size of the transmission bottleneck between humans infected with the influenza virus; however, the methods used to make these est...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00014-20
更新日期:2020-06-16 00:00:00
abstract::Measles virus (MeV), like all viruses of the order Mononegavirales, utilizes a complex consisting of genomic RNA, nucleoprotein, the RNA-dependent RNA polymerase, and a polymerase cofactor, the phosphoprotein (P), for transcription and replication. We previously showed that a recombinant MeV that does not express anot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01733-19
更新日期:2020-01-31 00:00:00
abstract::Nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) possesses an RNA-dependent RNA polymerase activity responsible for viral genome RNA replication. Despite several reports on the characterization of this essential viral enzyme, little is known about the reaction pathway of NS5B-catalyzed nucleotide incorporati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9134-9143.2000
更新日期:2000-10-01 00:00:00
abstract::Tryptic peptide maps were prepared for four purified structural proteins derived from several murine leukemia viruses (MuLV's). Analyses of these peptide maps reveal that the p30 proteins of Rauscher, Moloney, and Gross MuLV's are very similar to each other, as are the p10's obtained from these three viruses. In contr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.5.1148-1157.1975
更新日期:1975-05-01 00:00:00
abstract::Enteroviral persistence has been implicated in the pathogenesis of several chronic human diseases, including dilated cardiomyopathy, insulin-dependent diabetes mellitus, and chronic inflammatory myopathy. However, these viruses are considered highly cytolytic, and it is unclear what mechanisms might permit their long-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4430-4440.2002
更新日期:2002-05-01 00:00:00
abstract::H5N1 and H9N2 avian influenza virus subtypes top the World Health Organization's list for the greatest pandemic potential. Inactivated H5N1 vaccines induce limited immune responses and, in the case of live-attenuated influenza virus vaccines (LAIV), there are safety concerns regarding the possibility of reassortment b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02490-12
更新日期:2013-05-01 00:00:00
abstract::Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5' untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02674-08
更新日期:2009-07-01 00:00:00
abstract::From a stock of varkappa phage grown on Salmonella, a host-range mutant which attacks Escherichia coli was isolated. As in the case of Salmonella, only motile strains of E. coli are sensitive to varkappa. The phage shows an eclipse period of 35 min and a minimal latent period of 52 min. The adsorption rate constant is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.3.591-598.1967
更新日期:1967-06-01 00:00:00
abstract::A map of bacteriophage lambda was constructed, including accurate positions for all 41 cut sites made by 12 different restriction enzymes. Over 100 fragments from single, multiple, and partial enzyme digestions were measured versus standards that were calibrated with respect to DNA molecules of known sequence. The dat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.390-400.1980
更新日期:1980-01-01 00:00:00