Abstract:
:Infectious bursal disease virus (IBDV), a double-stranded RNA (dsRNA) virus belonging to the Birnaviridae family, is an economically important avian pathogen. The IBDV capsid is based on a single-shelled T=13 lattice, and the only structural subunits are VP2 trimers. During capsid assembly, VP2 is synthesized as a protein precursor, called pVP2, whose 71-residue C-terminal end is proteolytically processed. The conformational flexibility of pVP2 is due to an amphipathic alpha-helix located at its C-terminal end. VP3, the other IBDV major structural protein that accomplishes numerous roles during the viral cycle, acts as a scaffolding protein required for assembly control. Here we address the molecular mechanism that defines the multimeric state of the capsid protein as hexamers or pentamers. We used a combination of three-dimensional cryo-electron microscopy maps at or close to subnanometer resolution with atomic models. Our studies suggest that the key polypeptide element, the C-terminal amphipathic alpha-helix, which acts as a transient conformational switch, is bound to the flexible VP2 C-terminal end. In addition, capsid protein oligomerization is also controlled by the progressive trimming of its C-terminal domain. The coordination of these molecular events correlates viral capsid assembly with different conformations of the amphipathic alpha-helix in the precursor capsid, as a five-alpha-helix bundle at the pentamers or an open star-like conformation at the hexamers. These results, reminiscent of the assembly pathway of positive single-stranded RNA viruses, such as nodavirus and tetravirus, add new insights into the evolutionary relationships of dsRNA viruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Luque D,Saugar I,Rodríguez JF,Verdaguer N,Garriga D,Martín CS,Velázquez-Muriel JA,Trus BL,Carrascosa JL,Castón JRdoi
10.1128/JVI.00077-07subject
Has Abstractpub_date
2007-07-01 00:00:00pages
6869-78issue
13eissn
0022-538Xissn
1098-5514pii
JVI.00077-07journal_volume
81pub_type
杂志文章abstract::A systematic analysis of immune responses on a population level is critical for a human immunodeficiency virus type 1 (HIV-1) vaccine design. Our studies in Botswana on (i) molecular analysis of the HIV-1 subtype C (HIV-1C) epidemic, (ii) frequencies of major histocompatibility complex class I HLA types, and (iii) cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.20.10155-10168.2002
更新日期:2002-10-01 00:00:00
abstract::Viral DNA sequences were not detected in high-molecular-weight host DNA until well after the onset of viral DNA replication. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.397-399.1979
更新日期:1979-04-01 00:00:00
abstract::CD8+ T cells were previously shown to be important in preventing lymphoproliferation and immunodeficiency following infection of murine AIDS (MAIDS)-resistant mice with the LP-BM5 mixture of murine leukemia viruses. To further evaluate the mechanisms contributing to MAIDS resistance, we studied mice lacking CD8+ T cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1808-1813.1997
更新日期:1997-03-01 00:00:00
abstract::The interferon response elicited by Salmonella typhimurium mutants in mice is not dependent on the presence of a complete cell wall lipopolysaccharide. In fact, a mutant (G30/C21) which has lost all the polysaccharide side chains and sugars of the O antigen and contains only 2-keto-3-deoxyoctonate and lipid is indisti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.6.1164-1167.1967
更新日期:1967-12-01 00:00:00
abstract::All herpesviruses encode a homolog of glycoprotein M (gM), which appears to function in virion morphogenesis. Despite its conservation, gM is inessential for the lytic replication of alphaherpesviruses. In order to address the importance of gM in gammaherpesviruses, we disrupted it in the murine gammaherpesvirus 68 (M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3459-3467.2005
更新日期:2005-03-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) latently infects KS tumors, primary effusion lymphomas (PELs), and PEL cell lines. K12 (T0.7) is the most abundant transcript expressed in latent KSHV infection. We characterize here the K12 transcript from a PEL tumor prior to passage in cell culture. The PEL tumor KSHV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.11880-11888.2002
更新日期:2002-12-01 00:00:00
abstract::Levels of the p53 tumor suppressor protein are increased in human cytomegalovirus (HCMV)-infected cells and may be important for HCMV pathogenesis. In normal cells p53 levels are kept low due to an autoregulatory feedback loop where p53 activates the transcription of mdm2 and mdm2 binds and ubiquitinates p53, targetin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3833-3843.2006
更新日期:2006-04-01 00:00:00
abstract::An assay to identify interactions between Citrus Dwarfing Viroid (CDVd) and Citrus Tristeza Virus (CTV) showed that viroid titer was enhanced by the coinfecting CTV in Mexican lime but not in etrog citron. Since CTV encodes three RNA silencing suppressors (RSSs), p23, p20 and p25, an assay using transgenic Mexican lim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02619-13
更新日期:2014-01-01 00:00:00
abstract::Hepatitis C virus (HCV) often causes a persistent infection associated with hypergammaglobulinemia, high levels of antiviral antibody and circulating immune complexes, and immune complex disease. We previously reported that only a limited neutralizing activity to vesicular stomatitis virus or HCV pseudotype is generat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01867-07
更新日期:2008-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) transactivates EBV genes in latently infected B cells. We have shown that mitotic hyperphosphorylation of EBNA2 suppresses its ability to transactivate the latent membrane protein 1 (LMP1) promoter. In this follow-up study, we identify EBNA2 Ser243 as a phosphorylatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.2045-2050.2006
更新日期:2006-02-01 00:00:00
abstract::Immature retroviral particles are assembled by self-association of the structural polyprotein precursor Gag. During maturation the Gag polyprotein is proteolytically cleaved, yielding mature structural proteins, matrix (MA), capsid (CA), and nucleocapsid (NC), that reassemble into a mature viral particle. Proteolytic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05564-11
更新日期:2012-02-01 00:00:00
abstract::We have previously identified 11 epitopes located in two topologically nonoverlapping antigenic sites (A and B) and a third bridging site (C) on the human type 3 parainfluenza virus (PIV3) hemagglutinin-neuraminidase (HN) glycoprotein by using monoclonal antibodies (MAbs) which inhibit hemagglutination and virus infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.90-96.1986
更新日期:1986-10-01 00:00:00
abstract::To study fundamental aspects of RNA recombination, an in vivo RNA recombination system was established. This system allowed the efficient generation of recombinant cytopathogenic pestiviruses after transfection of synthetic, nonreplicatable, subgenomic transcripts in cells infected with a replicating noncytopathogenic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6271-6281.2004
更新日期:2004-06-01 00:00:00
abstract::The endogenous transcriptase present in purified vesicular stomatitis (VS) virions was solubilized with a Triton X-100 high-salt solution. The polymerase activity was purified on glycerol gradients and by phosphocellulose column chromatography; the viral proteins present in the active enzyme fractions were identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1325-1335.1973
更新日期:1973-12-01 00:00:00
abstract::When short pulses of [(3)H]uracil were administered to Bacillus subtilis infected with phage 2C, the main species of labeled RNA was a 10S component that hybridized chiefly, but not exclusively, with the heavy strand of 2C DNA. After long pulses, most of the radioactivity was found in the 23S, 16S, and 5S rRNA's, whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.6.1482-1493.1974
更新日期:1974-12-01 00:00:00
abstract::Hepatitis C virus (HCV) is a single-stranded positive-sense RNA hepatotropic virus. Despite cellular defenses, HCV is able to replicate in hepatocytes and to establish a chronic infection that could lead to severe complications and hepatocellular carcinoma. An important player in subverting the host response to HCV in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00880-17
更新日期:2017-08-24 00:00:00
abstract::Cytomegalovirus (CMV) disease after bone marrow (BM) transplantation is often associated with BM graft failure. There are two possible reasons for such a correlation. First, a poor hematopoietic reconstitution of unrelated etiology could promote the progression of CMV infection by the lack of immune control. Alternati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.5006-5015.1998
更新日期:1998-06-01 00:00:00
abstract::Adenovirus types 5 and 3 (Ad5 and Ad3), two human adenovirus serotypes of evolutionarily divergent subgroups, show very different levels of E1A gene expression early after infection of permissive cells. Since adenovirus E1A gene expression is known to be transcriptionally autoregulated, we have investigated the differ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1833-1838.1989
更新日期:1989-04-01 00:00:00
abstract::Although several virologic and immunologic factors associated with an increased risk of perinatal human immunodeficiency virus type 1 (HIV-1) transmission have been described, the mechanism of mother-to-child transmission is still unclear. More specifically, the question of whether selective pressures influence the tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3050-3057.2003
更新日期:2003-03-01 00:00:00
abstract::Antisera produced in mice recognize primarily type-specific antigenic determinants on both the major core protein, p30, and the major envelope proteins, gp70 and p15(E), of the endogenous leukemia viruses (MuLV) of BALB/c mice. Three different mouse sera were investigated in detail. (i) Antisera prepared in C57BL/6 mi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.345-349.1979
更新日期:1979-10-01 00:00:00
abstract::Fluorosulfonylbenzoyl 5'-adenosine (FSBA) bound to one site in simian virus 40 large T antigen (T) and covalently modified greater than 95% of the molecules in a complete reaction. This analog for ATP specifically cross-links to the Mg-phosphate pocket in ATP-binding sites. Cyanogen bromide cleavage and tryptic digest...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4939-4947.1990
更新日期:1990-10-01 00:00:00
abstract::To determine which sequences or structures in the poliovirus 5' noncoding region (5'NCR) are involved in binding proteins used for internal ribosome binding and protein synthesis initiation, translation competition assays were performed in rabbit reticulocyte lysates in the presence and absence of HeLa cell extract. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.3101-3109.1992
更新日期:1992-05-01 00:00:00
abstract::Rabies virus (RV) of the Rhabdoviridae family grows in alpha/beta interferon (IFN)-competent cells, suggesting the existence of viral mechanisms preventing IFN gene expression. We here identify the viral phosphoprotein P as the responsible IFN antagonist. The critical involvement of P was first suggested by the observ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7673-7681.2005
更新日期:2005-06-01 00:00:00
abstract::Epstein-Barr (EBV) is a powerful immortalizing virus for human B lymphocytes in vitro and is associated with several human neoplasias in vivo. Previously, we have shown that the majority of EBV-infected cells in the peripheral blood of healthy, persistently infected individuals do not express the activated phenotype, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.4882-4891.1997
更新日期:1997-07-01 00:00:00
abstract::Ribonucleic acid extracted from the virions of two human rhinoviruses is infective and is similar in size to poliovirus ribonucleic acid. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.2.278-280.1971
更新日期:1971-02-01 00:00:00
abstract::Phage P22 can integrate as prophage into a recombination-deficient (Rec(-)) strain of Salmonella typhimurium. At 37 C, the integration efficiency is only 10% that in Rec(+) infection, but at 25 C the efficiencies in Rec(-) and Rec(+) hosts are similar. Rec(-) lysogens cannot be induced by ultraviolet irradiation or by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.7.702-709.1968
更新日期:1968-07-01 00:00:00
abstract::Galectin-9 is a pleiotropic immune modulator affecting numerous cell types of innate and adaptive immunity. Patients with chronic infection with either hepatitis C virus (HCV) or HIV have elevated circulating levels. Limited data exist on the regulation of natural killer (NK) cell function through interaction with gal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01085-12
更新日期:2013-05-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) of macaques isolate SIVmac239 is highly resistant to neutralization by polyclonal antisera or monoclonal antibodies, a property that it shares with most primary isolates of human immunodeficiency virus type 1 (HIV-1). This resistance is important for the ability of the virus to pers...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.9993-10003.2003
更新日期:2003-09-01 00:00:00
abstract::ICP27 is a multifunctional protein that is required for herpes simplex virus 1 mRNA export. ICP27 interacts with the mRNA export receptor TAP/NXF1 and binds RNA through an RGG box motif. Unlike other RGG box proteins, ICP27 does not bind G-quartet structures but instead binds GC-rich sequences that are flexible in str...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00509-10
更新日期:2010-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) strains can be separated into genetic subtypes based on phylogenetic analysis of the envelope gene. Once it had been shown that population-wide intrasubtype genetic variation of HIV-1 strains increases in the course of the AIDS epidemic, it remained uncertain whether HIV-1 s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6332-6338.1997
更新日期:1997-09-01 00:00:00