Abstract:
:Epstein-Barr (EBV) is a powerful immortalizing virus for human B lymphocytes in vitro and is associated with several human neoplasias in vivo. Previously, we have shown that the majority of EBV-infected cells in the peripheral blood of healthy, persistently infected individuals do not express the activated phenotype, e.g., high levels of cell surface CD23 and CD80 (B7), characteristically expressed on in vitro-immortalized cells. Here, we show that > or = 90% of the CD23-, virus-infected cells in the peripheral blood are in G0 and therefore resting. The remaining cells may be G1 arrested, but we were unable to detect a significant number of cells traversing the S-G2-M stages of the cell cycle. The mRNA for LMP2A, but not EBNA1 originating from Qp, was readily detected in this population, and these cells appear competent in the processing and presentation of antigen by class I major histocompatibility complex. We propose that these resting B cells are the site of long-term latent persistence for EBV. We further propose that the persistence of the virus in a resting B7- B cell provides an important mechanism to escape immunosurveillance. The demonstration that EBV can persist latently in a resting B cell means that the immortalizing functions of EBV can be down regulated in a normal B cell. This conclusion has important implications for understanding and controlling EBV-associated neoplasia.
journal_name
J Viroljournal_title
Journal of virologyauthors
Miyashita EM,Yang B,Babcock GJ,Thorley-Lawson DAdoi
10.1128/JVI.71.7.4882-4891.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
4882-91issue
7eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::Transcription of the terminal protein (TP) gene of Epstein-Barr virus (EBV) in Burkitt's lymphoma cells, in EBV-negative Burkitt's lymphoma cells converted with transformation-defective (P3HR1) and transformation-competent (B95-8, AG876) EBV strains, and in EBV-immortalized cell lines was studied. A TP1 cDNA probe spa...
journal_title:Journal of virology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1980-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.75.13.6242-6244.2001
更新日期:2001-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.68.4.2347-2354.1994
更新日期:1994-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1991-12-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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