Abstract:
:Effectively recognizing invading viruses and subsequently inducing innate antiviral immunity are essential for host antiviral defense. Although these processes are closely regulated by the host to maintain immune balance, viruses have evolved the ability to downregulate or upregulate these processes for their survival. MicroRNAs (miRNAs) are a family of small noncoding RNAs that play vital roles in modulating host immune response. Accumulating evidence demonstrates that host miRNAs as mediators are involved in regulating viral replication and host antiviral immunity in mammals. However, the underlying regulatory mechanisms in fish species are still poorly understood. Here, we found that rhabdovirus infection significantly upregulated host miR-3570 expression in miiuy croaker macrophages. Induced miR-3570 negatively modulated RNA virus-triggered type I interferon (IFN) and antiviral gene production, thus facilitating viral replication. Furthermore, miR-3570 was found to target and posttranscriptionally downregulate mitochondrial antiviral signaling protein (MAVS), which functions as a platform for innate antiviral signal transduction. Moreover, we demonstrated that miR-3570 suppressed the expression of MAVS, thereby inhibiting MAVS-mediated NF-κB and IRF3 signaling. The collective results demonstrated a novel regulation mechanism of MAVS-mediated immunity during RNA viral infection by miRNA.IMPORTANCE RNA viral infection could upregulate host miR-3570 expression in miiuy croaker macrophages. Induced miR-3570 negatively modulates RNA virus-triggered type I IFN and antiviral gene production, thus facilitating viral replication. Remarkably, miR-3570 could target and inhibit MAVS expression, which thus modulates MAVS-mediated NF-κB and IRF3 signaling. The collective results of this study suggest a novel regulation mechanism of MAVS-mediated immunity during RNA viral infection by miR-3570. Thus, a novel mechanism for virus evasion in fish is proposed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Xu T,Chu Q,Cui J,Bi Ddoi
10.1128/JVI.01594-17subject
Has Abstractpub_date
2018-01-02 00:00:00issue
2eissn
0022-538Xissn
1098-5514pii
JVI.01594-17journal_volume
92pub_type
杂志文章abstract::In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02339-07
更新日期:2008-02-01 00:00:00
abstract::Type I interferons (IFNs), as major components of the innate immune system, play a vital role in host resistance to a variety of pathogens. Canonical signaling mediated by type I IFNs activates the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway through binding to the IFN-α/β receptor ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01161-18
更新日期:2018-09-12 00:00:00
abstract::A mouse model of high-risk human papillomavirus infection was developed in which human papillomavirus (HPV) type 16 DNA was inoculated into human foreskin grafted to the skin of severe combined immunodeficient (scid) mice. Grafted skin contained human epidermis and dermis and, like normal human skin, expressed involuc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2716-2721.1995
更新日期:1995-04-01 00:00:00
abstract::Gammaretroviruses, such as murine leukemia virus (MLV), are functionally distinguished from lentiviruses, such as human immunodeficiency virus, by their inability to infect nondividing cells. Attempts to engineer this property into MLV have been hindered by an incomplete understanding of early events in the viral life...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00615-06
更新日期:2006-09-01 00:00:00
abstract::Analyses of prototype vesicular stomatitis (VSV, Indiana serotype) mRNA-32P-labeled viral RNA duplexes have established the assignments of 65 of the 72 large oligonucleotides that are recovered by two-dimensional electrophoresis of RNase T1 digests of the viral RNA. Fifty of the oligonucleotides are recovered in the L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.116-123.1979
更新日期:1979-04-01 00:00:00
abstract::We cloned and analyzed the integrated human papillomavirus type 16 (HPV-16) genomes that are present in the human cervical carcinoma cell lines SiHa and CaSki. The single HPV-16 genome in the SiHa line was cloned as a 10-kilobase (kb) HindIII fragment. Integration of the HPV-16 genome occurred at bases 3132 and 3384 w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.962-971.1987
更新日期:1987-04-01 00:00:00
abstract::Herpes simplex virus type 2 (HSV-2) glycoprotein B (gB-2) gene segments were expressed as recombinant proteins in Escherichia coli. gB-2 recombinant proteins were reacted with human serum immunoglobulin G (IgG) antibodies in Western immunoblot assays. Initially, samples were tested for the presence of HSV-1-specific a...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.70.5.2950-2956.1996
更新日期:1996-05-01 00:00:00
abstract::Most mammalian rotaviruses contain tripeptide amino acid sequences in outer capsid proteins VP4 and VP7 which have been shown to act as ligands for integrins alpha2beta1 and alpha4beta1. Peptides containing these sequences and monoclonal antibodies directed to these integrins block rotavirus infection of cells. Here w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.1.228-236.2000
更新日期:2000-01-01 00:00:00
abstract::The unique region of the capsid protein VP1 (VP1u) of human parvovirus B19 (B19) elicits a dominant immune response and has a phospholipase A(2) (PLA(2)) activity, which is necessary for the infection. In contrast to the rest of the parvoviruses, the VP1u of B19 is thought to occupy an external position in the virion,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01435-06
更新日期:2006-12-01 00:00:00
abstract::Combined Southern blot hybridization analyses of DNA digests of several clinical strains of Streptococcus pneumoniae have revealed the presence of a gene (hblR), or part of it, similar to the hbl7 gene coding for the cell wall lytic enzyme of the temperate HB-746 phage. The results confirmed that the genome of HB-746,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.2860-2864.1992
更新日期:1992-05-01 00:00:00
abstract::Herpes simplex virus (HSV) latency in neurons remains poorly understood, and the heterogeneity of the sensory nervous system complicates mechanistic studies. In this study, we used primary culture of adult trigeminal ganglion (TG) mouse neurons in microfluidic devices and an in vivo model to examine the subtypes of se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00430-18
更新日期:2018-06-13 00:00:00
abstract::HBI is a recombinant avian retrovirus with novel pathogenic properties that was derived from the myc-containing virus MC29. In contrast to MC29, which causes endotheliomas in chickens, HBI induces lymphoid tumors. The results of molecular cloning and nucleotide sequencing of HBI reported here show that the virus conta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.3.969-977.1985
更新日期:1985-12-01 00:00:00
abstract::Cleavage of varicella-zoster virus DNA with the restriction endonucleases PstI, XbaI, and BglII resulted in 18, 22, and 20 fragments, respectively. Based on the molecular weights and molarities of these fragments, a molecular weight of 84 x 10(6) could be calculated for the varicella-zoster virus genome. In both the X...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.390-400.1981
更新日期:1981-08-01 00:00:00
abstract::The parvovirus minute virus of mice (MVM) packages predominantly negative-sense single strands, while its close relative LuIII encapsidates strands of both polarities with equal efficiency. Using genomic chimeras and mutagenesis, we show that the ability to package positive strands maps not, as originally postulated, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2287-2300.2005
更新日期:2005-02-01 00:00:00
abstract::The nucleotide and deduced amino acid sequences of the P1 region of the genomes of 10 independent equine rhinitis A virus (ERAV) isolates were determined and found to be very closely related. A panel of seven monoclonal antibodies to the prototype virus ERAV.393/76 that bound to nonneutralization epitopes conserved am...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10550-10556.2001
更新日期:2001-11-01 00:00:00
abstract::Virus-specific CD4(+) T-cell function is thought to play a central role in induction and maintenance of effective CD8(+) T-cell responses in experimental animals or humans. However, the reasons that diminished proliferation of human immunodeficiency virus (HIV)-specific CD4(+) T cells is observed in the majority of in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.20.10900-10909.2003
更新日期:2003-10-01 00:00:00
abstract:UNLABELLED:Venezuelan equine encephalitis virus (VEEV) is an important human and animal pathogen, for which no safe and efficient vaccines or therapeutic means have been developed. Viral particle assembly and budding processes represent potential targets for therapeutic intervention. However, our understanding of the m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02680-15
更新日期:2015-12-09 00:00:00
abstract::Mumps is a highly contagious human disease, characterized by lateral or bilateral nonsuppurative swelling of the parotid glands and neurological complications that can result in aseptic meningitis or encephalitis. A mumps vaccination program implemented since the 1960s reduced mumps incidence by more than 99% and kept...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02778-13
更新日期:2014-03-01 00:00:00
abstract::A 4,500-bp BamHI fragment, located within the HindIII A segment of the vaccinia virus genome, was found to contain eight potential coding regions for polypeptides of 78 to 346 amino acids. The open reading frames with 133, 346, and 125 codons were homologous to profilin (an actin-binding protein), 3-beta-hydroxysteroi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4598-4608.1991
更新日期:1991-09-01 00:00:00
abstract::The interferon response elicited by Salmonella typhimurium mutants in mice is not dependent on the presence of a complete cell wall lipopolysaccharide. In fact, a mutant (G30/C21) which has lost all the polysaccharide side chains and sugars of the O antigen and contains only 2-keto-3-deoxyoctonate and lipid is indisti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.6.1164-1167.1967
更新日期:1967-12-01 00:00:00
abstract::High-risk human papillomavirus type 16 (HPV16) is the primary causative agent of cervical cancer and therefore is responsible for significant morbidity and mortality worldwide. Cellular transformation is mediated directly by the expression of viral oncogenes, the least characterized of which, E5, subverts cellular pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06243-11
更新日期:2012-05-01 00:00:00
abstract::Transcriptionally active replication complexes bound to smooth membrane vesicles were isolated from poliovirus-infected cells. In electron microscopic, negatively stained preparations, the replication complex appeared as an irregularly shaped, oblong structure attached to several virus-induced vesicles of a rosettelik...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1156-1163.1990
更新日期:1990-03-01 00:00:00
abstract::The E3 complex transcription unit of adenovirus encodes four major mRNAs (a, c, f, and h) and two minor (d and e) mRNAs with overlapping exons, alternative splice sites, and two polyadenylation sites, termed E3A (upstream) and E3B (downstream). mRNAs a and d use the E3A polyadenylation site, and mRNAs c, e, f, and h u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5914-5923.1992
更新日期:1992-10-01 00:00:00
abstract::The region of the UL component of the herpes simplex virus type 1 genome between nucleotides 17,793 and 25,150 includes three open reading frames that code for the protein products of the UL8, UL9, and UL10 genes (D.J. McGeogh, M.A. Dalrymple, A.J. Davison, A. Dolan, M.C. Frame, D. McNab, L.J. Perry, J.E. Scott, and P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4251-4261.1994
更新日期:1994-07-01 00:00:00
abstract::Three distinct strains of murine rotavirus were adapted to growth in cell culture. These strains are genetically related but not identical; they are serotypically heterogeneous. The cultivatable strains were substantially more infectious (approximately 10(6)-fold) for suckling mice than heterologous simian rotaviruses...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.2.585-590.1986
更新日期:1986-02-01 00:00:00
abstract::The mosquito-transmitted dengue virus (DENV) infects millions of people in tropical and subtropical regions. Maturation of DENV particles requires proper cleavage of the viral polyprotein, including processing of 8 of the 13 substrate cleavage sites by dengue virus NS2B/NS3 protease. With no available direct-acting an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00045-17
更新日期:2017-04-28 00:00:00
abstract::The mitogen-activated protein (MAP) kinase signal transduction pathway is an intracellular signaling cascade which mediates cellular responses to growth and differentiation factors. The MAP kinase pathway can be activated by a wide range of stimuli dependent on the cell types, and this is normally a transient response...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.8051-8056.1995
更新日期:1995-12-01 00:00:00
abstract::Norovirus (NoV) infections are a significant health burden to society, yet the lack of reliable tissue culture systems has hampered the development of appropriate antiviral therapies. Here we show that the NoV NS3 protein, derived from murine NoV (MNV), is intimately associated with the MNV replication complex and the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02138-16
更新日期:2017-01-18 00:00:00
abstract::The lymphocytic choriomeningitis virus (LCMV) genome consists of a large RNA segment and a small RNA segment. The three major structural proteins of this virus are an internal nucleoprotein and two surface glycoproteins. Intertypic reassortants between the Armstrong and WE strains of LCMV were made to map proteins enc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.3.966-968.1985
更新日期:1985-03-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) primary infection is characterized by the use of CCR5 as a coreceptor for viral entry, which is associated with the non-syncytium-inducing (NSI) phenotype in lymphoid cells. Syncytium-inducing (SI) variants of HIV-1 appear in advanced stages of HIV-1 infection and are charac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10319-10325.2001
更新日期:2001-11-01 00:00:00