Abstract:
:Cytoplasmic virus-specific RNA and polyribosomes from a chronically infected feline thymus tumor cell line, F-422, were analyzed by using in vitro-synthesized feline leukemia virus (Rickard strain) (R-FeLV) complementary DNA (cDNA) probe. By hybridization kinetics analysis, cytoplasmic, polyribosomat, and nuclear RNAs were found to be 2.1, 2.6, and 0.7% virus specific, respectively. Size classes within subcellular fractions were determined by sucrose gradient centrifugation in the presence of dimethyl sulfoxide followed by hybridization. The cytoplasmic fraction contained a 28S size class, which corresponds to the size of virion subunit RNA, and 36S, 23S, and 15 to 18S RNA species. The virus-specific 36S, 23S, and 15 to 18S species but not the 28S RNA were present in both the total and polyadenylic acid-containing polyribosomal RNA. Anti-FeLV gamma globulin bound to rapidly sedimenting polyribosomes, with the peak binding at 400S. The specificity of the binding for nascent virus-specific protein was determined in control experiments that involved mixing polyribosomes with soluble virion proteins, absorption of specific gamma globulin with soluble virion proteins, and puromycin-induced nascent protein release. The R-FeLV cDNA probe hybridized to RNA in two polyribosomal regions (approximately 400 to 450S and 250S) within the polyribosomal gradients before but not after EDTA treatment. The 400 to 450S polyribosomes contained three major peaks of virus-specific RNA at 36S, 23S, and 15 to 18S, whereas the 250S polyribosomes contained predominantly 36S and 15 to 18S RNA. Further experiments suggest that an approximately 36S minor subunit is present in virion RNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Conely AJ,Velicer LFdoi
10.1128/JVI.25.3.750-763.1978subject
Has Abstractpub_date
1978-03-01 00:00:00pages
750-63issue
3eissn
0022-538Xissn
1098-5514journal_volume
25pub_type
杂志文章abstract::Currently there is limited information about the quality of immune responses elicited by candidate human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env)-based immunogens in primates. Here we describe a comprehensive analysis of neutralizing antibody and T-cell responses obtained in cynomolgus macaque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01102-08
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5522-5528.1993
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pub_type: 杂志文章
doi:10.1128/JVI.37.3.1094-1098.1981
更新日期:1981-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.2163-2170.1997
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journal_title:Journal of virology
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.9817-9822.2003
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.1.460-473.2006
更新日期:2006-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15494-15502.2005
更新日期:2005-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.591-593.1984
更新日期:1984-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.3040-3042.1988
更新日期:1988-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2615-2623.1994
更新日期:1994-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.72.7.5978-5983.1998
更新日期:1998-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1977-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12369-12375.2002
更新日期:2002-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.65.4.1905-1909.1991
更新日期:1991-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.6.1367-1377.1975
更新日期:1975-06-01 00:00:00