Abstract:
:The Kaposi's sarcoma-associated herpesvirus nuclear egress complex is composed of two proteins, ORF67 and ORF69. In this study, we have recapitulated the KSHV complex by coexpression of these two proteins in insect cells using expression from recombinant baculoviruses. The proteins form a complex at the nuclear membrane as judged by live-cell analysis of protein fusions tagged with green fluorescent protein (GFP) and mCherry. Ultrastructural analysis of infected cells showed that ORF67 expression results in reduplication of the nuclear membrane. When the two proteins are expressed together, numerous virion-size nuclear membrane-derived vesicles were evident at the nuclear margins.
journal_name
J Viroljournal_title
Journal of virologyauthors
Desai PJ,Pryce EN,Henson BW,Luitweiler EM,Cothran Jdoi
10.1128/JVI.05988-11subject
Has Abstractpub_date
2012-01-01 00:00:00pages
594-8issue
1eissn
0022-538Xissn
1098-5514pii
JVI.05988-11journal_volume
86pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
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