Abstract:
:Foot-and-mouth disease virus (FMDV), a member of the Picornaviridae, is a pathogen of cloven-hoofed animals and causes a disease of major economic importance. Picornavirus-infected cells show changes in cell morphology and rearrangement of cytoplasmic membranes, which are a consequence of virus replication. We show here, by confocal immunofluorescence and electron microscopy, that the changes in morphology of FMDV-infected cells involve changes in the distribution of microtubule and intermediate filament components during infection. Despite the continued presence of centrosomes in infected cells, there is a loss of tethering of microtubules to the microtubule organizing center (MTOC) region. Loss of labeling for gamma-tubulin, but not pericentrin, from the MTOC suggests a targeting of gamma-tubulin (or associated proteins) rather than a total breakdown in MTOC structure. The identity of the FMDV protein(s) responsible was determined by the expression of individual viral nonstructural proteins and their precursors in uninfected cells. We report that the only viral nonstructural protein able to reproduce the loss of gamma-tubulin from the MTOC and the loss of integrity of the microtubule system is FMDV 3C(pro). In contrast, infection of cells with another picornavirus, bovine enterovirus, did not affect gamma-tubulin distribution, and the microtubule network remained relatively unaffected.
journal_name
J Viroljournal_title
Journal of virologyauthors
Armer H,Moffat K,Wileman T,Belsham GJ,Jackson T,Duprex WP,Ryan M,Monaghan Pdoi
10.1128/JVI.00907-08subject
Has Abstractpub_date
2008-11-01 00:00:00pages
10556-66issue
21eissn
0022-538Xissn
1098-5514pii
JVI.00907-08journal_volume
82pub_type
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2667-2673.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.4.1381-1387.1988
更新日期:1988-04-01 00:00:00
abstract:UNLABELLED:Recent evidence suggests that even in treated infections, human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) replication may continue in lymph nodes (LN), serving as a potential virus reservoir. Here we investigated the effects of lentivirus infection on natural killer (NK) cell frequ...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01203-06
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02395-08
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pub_type: 杂志文章
doi:10.1128/JVI.03771-13
更新日期:2014-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.25.1.274-284.1978
更新日期:1978-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01324-18
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.709-716.1976
更新日期:1976-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.663-672.1981
更新日期:1981-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00748-16
更新日期:2016-09-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.516-525.1987
更新日期:1987-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.741-747.1984
更新日期:1984-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00799-14
更新日期:2014-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.983-991.1986
更新日期:1986-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-09-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2806-2814.1998
更新日期:1998-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.1.109-112.1969
更新日期:1969-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.746-749.1997
更新日期:1997-01-01 00:00:00
abstract::The relationship between the vaccinia virus hemagglutinin and hemadsorption was examined. Hemagglutinin synthesis was temporally related to the appearance of the hemadsorption reaction. Only chicken erythrocytes, which reacted with hemagglutinin, hemadsorbed to infected cells, and both of these reactions were inhibite...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.2.290-296.1972
更新日期:1972-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.2.417-423.1985
更新日期:1985-08-01 00:00:00
abstract::A particles with the diameter of 70 to 80 nm were isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type. Most of the A particles banded at 1.23 to 1.24 g/ml, whereas 3 to 10% banded at 1.29 g/ml in equilibrium sucrose gradients. They banded at 1.30 g/ml in CsCl gradi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.4.924-933.1974
更新日期:1974-10-01 00:00:00
abstract::Analysis of the complete sequence of a human immunodeficiency virus (HIV) isolate (Ant70) obtained from a Cameroonian patient indicates that this virus is the most divergent strain within the HIV-1 family hitherto described. Comparison of the Pol protein, usually highly conserved within the HIV-1 family, shows only ab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1586-1596.1994
更新日期:1994-03-01 00:00:00
abstract::In vitro mapping studies of the MD145 norovirus (Caliciviridae) ORF1 polyprotein identified two stable cleavage products containing the viral RNA-dependent RNA polymerase (RdRp) domains: ProPol (a precursor comprised of both the proteinase and polymerase) and Pol (the mature polymerase). The goal of this study was to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2393-2403.2005
更新日期:2005-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.2.381-388.1977
更新日期:1977-05-01 00:00:00
abstract::The biologically relevant form of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) glycoprotein is oligomeric, with the major points of contact between oligomeric partners located in the ectodomain of gp41. To identify and map conserved epitopes and regions in gp41 where structure is influenced by quater...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.2674-2684.1997
更新日期:1997-04-01 00:00:00
abstract::The viral early-to-late switch of papillomavirus infection is tightly linked to keratinocyte differentiation and is mediated in part by alternative mRNA splicing. Here, we report that SRp20, a cellular splicing factor, controls the early-to-late switch via interactions with A/C-rich RNA elements. An A/C-rich SE4 eleme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01719-08
更新日期:2009-01-01 00:00:00