Abstract:
:A specific endoribonucleolytic activity was detected when detergent-lysed vesicular stomatitis of Sendai virus was incubated with the precursor to Escherichia coli tRNA Tyr. The cleavage products produced and the characteristics of the reaction were similar to those previously reported for human KB cell RNase NU. Like RNase NU, the virus-associated reaction generates 5'-hydroxyl and 3'-phosphate groups at the cleavage sites. At protein concentrations similar to those used to test vesicular stomatitis and Sendai viruses, virions of Sindbis virus and poliovirus also exhibited endoribonucleolytic activity, but reovirus, simian virus 40, and minute virus of mice did not. This endoribonuclease may be of physiological relevance to some of the viruses we tested.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kolakofsky D,Altman Sdoi
10.1128/JVI.25.1.274-284.1978subject
Has Abstractpub_date
1978-01-01 00:00:00pages
274-84issue
1eissn
0022-538Xissn
1098-5514journal_volume
25pub_type
杂志文章abstract::Purified preparations of human adenovirus type 12 showed two bands when subjected to isopycnic centrifugation in a density gradient of cesium chloride. Their density difference was about 0.003 g/ml, suggesting a small difference in their deoxyribonucleic acid to protein ratio. Virions with a lighter density can kill h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.4.426-433.1971
更新日期:1971-04-01 00:00:00
abstract::The S2 gene of bluetongue virus, serotype 17, has been cloned, and the nonstructural protein NS2 has been expressed. Synthetic peptides matching regions within the amino acid sequence of NS2 were used to map three single-stranded RNA (ssRNA)-binding regions within the protein. A prokaryotic expression system was then ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.2.499-506.2002
更新日期:2002-01-01 00:00:00
abstract::The coding properties of RNA transcribed in vitro by purified vaccinia cores have been investigated using Krebs ascites tumor cells, L cells, and reticulocyte lysates. Six to 10 proteins synthesized in vitro are separated on polyacrylamide gels by electrophoresis in the presence of sodium dodecyl sulfate. Their molecu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.6.1467-1474.1975
更新日期:1975-06-01 00:00:00
abstract::Two foot-and-mouth disease virus (FMDV) genome sequences have been determined for isolates collected from recent field outbreaks in North Africa (Egypt) and the Middle East (Palestinian Autonomous Territories). These data represent the first examples of complete genomic sequences for the FMDV SAT 2 topotype VII, which...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01231-12
更新日期:2012-08-01 00:00:00
abstract::Cell-free translation of separated Sendai virus mRNA species identified the message for polypeptide M and suggested the identity of the message for polypeptide NP. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.411-414.1978
更新日期:1978-10-01 00:00:00
abstract::We present data that retroviral gene expression in early hematopoietic cells is subjected to transcriptional controls similar to those previously described for embryonic stem cells. Transient transfection experiments revealed that both the viral enhancer region in the U3 region of the long terminal repeat as well as a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7541-7547.1995
更新日期:1995-12-01 00:00:00
abstract::Aluminum (Al)-based salts are widely used adjuvants in ruminants and other species to strengthen the immune response elicited against vaccine antigen(s). However, they can lead to the formation of long-lasting granulomas composed of abundant activated macrophages. Small ruminant lentiviruses (SRLV) are widely distribu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01859-20
更新日期:2020-12-22 00:00:00
abstract::We analyzed wild mouse DNAs for the number and type of proviral genes related to the env sequences of various murine leukemia viruses (MuLVs). Only Mus species closely related to laboratory mice carried these retroviral sequences, and the different subclasses of viral env genes tended to be restricted to specific taxo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3082-3088.1987
更新日期:1987-10-01 00:00:00
abstract::The regulation of murine cytomegalovirus early (E) gene expression was studied in the cell line B25, which is stably transfected with the immediate-early ie1/ie3 gene complex. Infection of B25 cells in the presence of the protein synthesis inhibitor cycloheximide resulted in the expression of some E genes, whereas for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.1907-1919.1990
更新日期:1990-05-01 00:00:00
abstract::Nucleocytoplasmic shuttling and interaction with the cellular mRNA export factor UAP56 are prerequisites for the mRNA export activity of human cytomegalovirus (HCMV) pUL69. Although the murine cytomegalovirus homolog pM69 shuttles, it fails to export mRNAs due to its inability to recruit UAP56. However, chimeric prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00730-12
更新日期:2012-07-01 00:00:00
abstract::Hepatitis C virus (HCV) infection remains a major worldwide health problem despite development of highly effective direct-acting antivirals. HCV rapidly evolves upon acute infection and generates multiple viral variants (quasispecies), leading to immune evasion and persistent viral infection. Identification of epitope...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00622-20
更新日期:2020-08-17 00:00:00
abstract::Hepatitis C virus (HCV) has two envelope proteins, E1 and E2, which form a heterooligomer. During dissection of interacting regions of HCV E1 and E2, we found the presence of an interfering compound or compounds in skim milk. Here we report that human as well as bovine lactoferrin, a multifunctional immunomodulator, b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.5997-6002.1997
更新日期:1997-08-01 00:00:00
abstract::Mosquito-borne alphaviruses, which replicate alternately and obligately in mosquitoes and vertebrates, appear to experience lower rates of evolution than do many RNA viruses that replicate solely in vertebrates. This genetic stability is hypothesized to result from the alternating host cycle, which constrains evolutio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14253-14260.2005
更新日期:2005-11-01 00:00:00
abstract::Virions produced by cells in the presence of actinomycin D (Act D virions) contain reverse transcriptase but are deficient in 70S genomic RNA. To assess the role of genomic RNA in encapsidation of a functional reverse transcriptase and to study the interaction of the enzyme and its template in the cores of intact viri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.2.478-488.1977
更新日期:1977-11-01 00:00:00
abstract::It has been reported that gene expression directed by the long terminal repeat of Rous sarcoma virus (RSV) is trans activated by a protein encoded in an alternate reading frame within the RSV gag gene (S. Broome and W. Gilbert, Cell 40:537-546, 1985). We have made specific mutations to test the role of the putative tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.11.4003-4008.1988
更新日期:1988-11-01 00:00:00
abstract::The lymphoid cell line #1670 has been derived from the infiltrated spleen of a tumor-bearing marmoset monkey infected with Herpesvirus saimiri. The cells contain both types of H. saimiri DNA, unique light (L-) DNA (36% cytosine plus guanine) and repetitive heavy (H-) DNA (71% cytosine plus guanine), without producing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.794-803.1977
更新日期:1977-06-01 00:00:00
abstract::Although shrews are one of the largest groups of mammals, little is known about their role in the evolution and transmission of viral pathogens, including coronaviruses (CoVs). We captured 266 Asian house shrews (Suncus murinus) in Jiangxi and Zhejiang Provinces, China, during 2013 to 2015. CoV RNA was detected in 24 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00764-17
更新日期:2017-08-10 00:00:00
abstract::Hepatitis C virus (HCV) is an enveloped positive-stranded RNA hepatotropic virus. HCV pseudoparticles infect liver-derived cells, supporting a model in which liver-specific molecules define HCV internalization. Three host cell molecules have been reported to be important entry factors or receptors for HCV internalizat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02286-07
更新日期:2008-05-01 00:00:00
abstract::Replication of flock house virus (FHV) RNA1 and production of subgenomic RNA3 in the yeast Saccharomyces cerevisiae provide a useful tool for the dissection of FHV molecular biology and host-encoded functions involved in RNA replication. The replication template activity of RNA1 can be separated from its coding potent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3905-3919.2002
更新日期:2002-04-01 00:00:00
abstract::Host cell-mediated proteolytic cleavage of the human immunodeficiency virus type 1 (HIV-1) gp160 precursor glycoprotein into gp120 and gp41 subunits is required to generate fusion-competent envelope glycoprotein (Env) spikes. The gp120-directed broadly neutralizing monoclonal antibodies (bNabs) isolated from HIV-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01247-13
更新日期:2013-12-01 00:00:00
abstract::Adeno-associated virus (AAV) integrates very efficiently into a specific site (AAVS1) of human chromosome 19. Two elements of the AAV genome are sufficient: the inverted terminal repeats (ITRs) and the Rep78 or Rep68 protein. The incorporation of the AAV integration machinery in nonviral delivery systems is of great i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7653-7658.1998
更新日期:1998-09-01 00:00:00
abstract::The hepatitis C virus (HCV) envelope proteins, E1 and E2, form noncovalent heterodimers and are leading candidate antigens for a vaccine against HCV. Studies in mammalian cell expression systems have focused primarily on E2 and its folding, whereas knowledge of E1 folding remains fragmentary. We used a cell-free in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.22.11205-11217.2001
更新日期:2001-11-01 00:00:00
abstract::The importance of neutralizing antibodies (NAbs) in protection against hepatitis C virus (HCV) remains controversial. We infused a chimpanzee with H06 immunoglobulin from a genotype 1a HCV-infected patient and challenged with genotype strains efficiently neutralized by H06 in vitro. Genotype 1a NAbs afforded no protec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01194-15
更新日期:2015-09-01 00:00:00
abstract::Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the complex cis-acting oriLyt region, which spans nearly 3 kb. DNA synthesis requires six core proteins together with UL84 and IE2. Previously, two essential regions were identified within oriLyt. Essential region I (nucleotides [nt] 92209 to 92573) ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00058-07
更新日期:2007-07-01 00:00:00
abstract::West Nile virus has spread rapidly across the United States, and there is currently no approved human vaccine or therapy to prevent or treat disease. Passive immunization with antibodies against the envelope protein represents a promising means to provide short-term prophylaxis and treatment for West Nile virus infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14606-14613.2005
更新日期:2005-12-01 00:00:00
abstract::The gastric mucosa is an important portal of entry for lymphocytic choriomeningitis virus (LCMV) infections. Within hours after intragastric (i.g.) inoculation, virus appears in the gastric epithelia, then in the mesenteric lymph nodes and spleen, and then in the liver and brain. By 72 h i.g.-inoculated virus is widel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8613-8619.1998
更新日期:1998-11-01 00:00:00
abstract::The matrix (MA) protein of human immunodeficiency virus type 1 (HIV-1) forms the outer protein shell directly underneath the lipid envelope of the virion. The MA protein has a key role in different aspects of virus assembly, including the incorporation of the HIV-1 Env protein complex, which contains a transmembrane g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3824-3830.1995
更新日期:1995-06-01 00:00:00
abstract::Epstein-Barr virus (EBV) undergoes latent and lytic replication cycles, and its reactivation from latency to lytic replication is initiated by expression of the two viral immediate-early transactivators, Zta and Rta. In vitro, reactivation of EBV can be induced by anti-immunoglobulin, tetradecanoyl phorbol acetate, an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01602-10
更新日期:2011-03-01 00:00:00
abstract::Marek's disease herpesvirus A antigen (MDHV-A) was identified as a 61,000- to 65,000-dalton glycoprotein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation from the culture medium of both [35S]methionine- and [14C]glucosamine-labeled infected cells by specific rabbit serum directed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.3.1228-1234.1983
更新日期:1983-03-01 00:00:00
abstract::The genetic evolution of human immunodeficiency virus type 1 (HIV-1) in the brain is distinct from that in lymphoid tissues, indicating tissue-specific compartmentalization of the virus. Few primary HIV-1 envelope glycoproteins (Envs) from uncultured brain tissues have been biologically well characterized. In this stu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12336-12345.2003
更新日期:2003-11-01 00:00:00