Abstract：
:The hepatitis C virus (HCV) envelope proteins, E1 and E2, form noncovalent heterodimers and are leading candidate antigens for a vaccine against HCV. Studies in mammalian cell expression systems have focused primarily on E2 and its folding, whereas knowledge of E1 folding remains fragmentary. We used a cell-free in vitro translation system to study E1 folding and asked whether the flanking proteins, Core and E2, influence this process. We translated the polyprotein precursor, in which the Core is N-terminal to E1, and E2 is C-terminal, and found that when the core protein was present, oxidation of E1 was a slow, E2-independent process. The half-time for E1 oxidation was about 5 h in the presence or absence of E2. In contrast with previous reports, analysis of three constructs of different lengths revealed that the E2 glycoprotein undergoes slow oxidation as well. Unfolded or partially folded E1 bound to the endoplasmic reticulum chaperones calnexin and (with lower efficiency) calreticulin, whereas no binding to BiP/GRP78 or GRP94 could be detected. Release from calnexin and calreticulin was used to assess formation of mature E1. When E1 was expressed in the absence of Core and E2, its oxidation was impaired. We conclude that E1 folding is a process that is affected not only by E2, as previously shown, but also by the Core. The folding of viral proteins can thus depend on complex interactions between neighboring proteins within the polyprotein precursor.
journal_name
J Viroljournal_title
Journal of virologyauthors
Merola M,Brazzoli M,Cocchiarella F,Heile JM,Helenius A,Weiner AJ,Houghton M,Abrignani Sdoi
10.1128/JVI.75.22.11205-11217.2001subject
Has Abstractpub_date
2001-11-01 00:00:00pages
11205-17issue
22eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
杂志文章abstract：:We previously reported that herpes simplex virus type 1 (HSV-1) can activate the stress-activated protein kinases (SAPKs) p38 and JNK. In the present study, we undertook a comprehensive and comparative analysis of the requirements for viral protein synthesis in the activation of JNK and p38. Infection with the UL36 mu...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.79.13.8348-8360.2005
更新日期：2005-07-01 00:00:00
abstract：UNLABELLED:Human noroviruses are icosahedral single-stranded RNA viruses. The capsid protein is divided into shell (S) and protruding (P) domains, which are connected by a flexible hinge region. There are numerous genetically and antigenically distinct noroviruses, and the dominant strains evolve every other year. Vacc...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.03176-14
更新日期：2015-03-01 00:00:00
abstract：:The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential protein. Previous studies have shown that the UL25 gene product is a virion component (M. A. Ali et al., Virology 216:278-283, 1996) involved in virus penetration and capsid assembly (C. Addison e...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.2.1060-1070.1998
更新日期：1998-02-01 00:00:00
abstract：:The purpose of this study was to determine whether a cell surface protein that can serve as coreceptor for herpes simplex virus type 1 (HSV-1) entry, herpesvirus entry mediator (previously designated HVEM but renamed HveA), also mediates HSV-1-induced cell-cell fusion. We found that transfection of DNA from KOS-804, a...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.7.5802-5810.1998
更新日期：1998-07-01 00:00:00
abstract：:We have analyzed the mechanism by which the matrix (M1) protein associates with cellular membranes during influenza A virus assembly. Interaction of the M1 protein with the viral hemagglutinin (HA) or neuraminidase (NA) glycoprotein was extensively analyzed by using wild-type and transfectant influenza viruses as well...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.70.10.6653-6657.1996
更新日期：1996-10-01 00:00:00
abstract：:The infection of human fetal thymus organ cultures (FTOC) with coxsackievirus B4 E2 (CVB4 E2) was investigated. Both positive- and negative-strand viral RNA were detected by real-time quantitative reverse transcription-PCR (RT-PCR) in CVB4 E2-infected FTOC, which supported high yields of virus production (approximatel...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.78.18.9854-9861.2004
更新日期：2004-09-01 00:00:00
abstract：:Monoclonal antibodies (MAb) reactive with the glycoprotein of vesicular stomatitis virus (VSV) serotypes Indiana (VSV-Ind) and New Jersey (VSV-NJ) were used to protect mice against lethal infection. MAb which reacted with a number of distinct epitopes and which could neutralize the virus in vitro could also protect ag...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.51.1.208-214.1984
更新日期：1984-07-01 00:00:00
abstract：:The pattern of induced protein species of the prototype strain of the parvovirus minute virus of mice was determined in permissive A9 mouse fibroblast cells by high-resolution two-dimensional gel electrophoresis. Identities of the viral proteins in the gels were assigned by probing two-dimensional blots with antisera ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.67.9.5126-5138.1993
更新日期：1993-09-01 00:00:00
abstract：:The human T-cell leukemia virus type 1 (HTLV-1) transforming protein, Tax, is a potent transactivator of both viral and cellular gene expression. The ability of Tax to transform cells is believed to depend on its transactivation of cellular-growth-regulatory genes. Expression of proliferating cell nuclear antigen (PCN...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.71.2.1181-1190.1997
更新日期：1997-02-01 00:00:00
abstract：:Bluetongue virus (BTV), a member of the Reoviridae family, is an insect-borne animal pathogen. Virus release from infected cells is predominantly by cell lysis, but some BTV particles are also released from the plasma membrane. The nonstructural protein NS3 has been implicated in this process. Using alternate initiato...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02352-10
更新日期：2011-05-01 00:00:00
abstract：:Epstein-Barr virus (EBV), a member of human gammaherpesvirus, infects mainly B cells. EBV has two alternative life cycles, latent and lytic, and is reactivated occasionally from the latent stage to the lytic cycle. To combat EBV-associated disorders, understanding the molecular mechanisms of the EBV lytic replication ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00975-17
更新日期：2017-11-14 00:00:00
abstract：:Cytoplasmic virus-specific RNA and polyribosomes from a chronically infected feline thymus tumor cell line, F-422, were analyzed by using in vitro-synthesized feline leukemia virus (Rickard strain) (R-FeLV) complementary DNA (cDNA) probe. By hybridization kinetics analysis, cytoplasmic, polyribosomat, and nuclear RNAs...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.25.3.750-763.1978
更新日期：1978-03-01 00:00:00
abstract：:The activation of the human polyomavirus BK causes polyomavirus-associated nephropathy in immunocompromised humans. Studies of the virus have been restricted since the virus DNA replication is species specific. Cell-based and cell-free DNA replication systems, including the BK virus (BKV) monopolymerase DNA replicatio...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00527-10
更新日期：2010-07-01 00:00:00
abstract：:An immunochemical analysis of the hemagglutinin (VP4) of the simian rotavirus SA11 was performed to better understand the structure and function of this molecule. Following immunization of mice with double-shelled virus particles and VP4-enriched fractions from CsCl gradients, a battery of anti-SA11 hybridomas was gen...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.62.6.2164-2172.1988
更新日期：1988-06-01 00:00:00
abstract：:Recent clinical trials examining 3'-azido-3'-deoxythymidine (AZT, zidovudine, or Retrovir) combined with L-2', 3'-dideoxy-3'-thiacytidine (3TC or lamivudine) have shown that combination therapy with these nucleoside analogs affords significant virological and clinical benefits. The addition of 3TC to AZT delays AZT re...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.6.5093-5098.1998
更新日期：1998-06-01 00:00:00
abstract：:Muscle is an attractive target for gene delivery because of its mass and because vectors can be delivered in a noninvasive fashion. Adeno-associated virus (AAV) has been shown to be effective for muscle-targeted gene transfer. Recent progress in characterization of AAV serotype 1 (AAV1) and AAV6 demonstrated that thes...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.77.4.2768-2774.2003
更新日期：2003-02-01 00:00:00
abstract：:The ATP-dependent DNase activity of Escherichia coli disappeared or was markedly reduced after infection with double-stranded DNA phages, T2, T3, T4, T5, T6, T7, lambda, phi80, and P1, but not with the single-stranded DNA phage f1, or the RNA phage Qbeta. This DNase activity was not reduced when chloramphenicol was ad...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.14.6.1611-1612.1974
更新日期：1974-12-01 00:00:00
abstract：:Sequencing and reversion analysis of murine hepatitis virus (MHV) temperature-sensitive (ts) viruses has identified putative ts mutations in the replicase nonstructural proteins (nsp's) of these coronaviruses. In this study, reverse transcriptase PCR sequencing of the RNA genome of an isolate of the MHV ts virus Alb t...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00203-08
更新日期：2008-06-01 00:00:00
abstract：:Rinderpest virus is a morbillivirus and is the causative agent of a widespread and important disease of cattle. The viral genome is a single strand of RNA in the negative sense. We have constructed plasmids containing cDNA copies of the 5' and 3' termini of the virus separated by a reporter gene and have shown that an...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.71.2.1265-1271.1997
更新日期：1997-02-01 00:00:00
abstract：UNLABELLED:The porcine sapovirus (SaV) (PoSaV) Cowden strain is one of only a few culturable enteric caliciviruses. Compared to the wild-type (WT) PoSaV Cowden strain, tissue culture-adapted (TC) PoSaV has two conserved amino acid substitutions in the RNA-dependent RNA polymerase (RdRp) and six in the capsid protein (V...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02197-15
更新日期：2015-11-18 00:00:00
abstract：:Adenovirus (Ad) entry into cells is initiated by the binding of the fiber knob to a cell surface receptor. The coxsackie- and adenovirus receptor (CAR) functions as the attachment receptor for many, but not all, Ad serotypes. Ad type 37 (Ad37), a subgroup D virus that causes keratoconjunctivitis in humans, does not in...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.75.11.5375-5380.2001
更新日期：2001-06-01 00:00:00
abstract：:A sequence of polyadenylic acid, homogeneous in composition but heterogeneous in length, was isolated from complete pancreatic RNase digests of both middle and bottom RNAs of cowpea mosaic virus. The polyadenylic acid was 3'-terminal and occurred once per molecule. A fragment consisting of the polyadenylic acid and ap...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.26.2.243-248.1978
更新日期：1978-05-01 00:00:00
abstract：:Coronaviruses are the largest RNA viruses, and their genomes encode replication machinery capable of efficient replication of both positive- and negative-strand viral RNAs as well as enzymes capable of processing large viral polyproteins into putative replication intermediates and mature proteins. A model described re...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00049-07
更新日期：2007-07-01 00:00:00
abstract：:The ICP18.5 gene (UL28) of herpes simplex virus type 1 is a member of a well-conserved gene family among herpesviruses and is thought to play a role in localization of viral glycoproteins. We have cloned, sequenced, and expressed the entire pseudorabies virus (PRV) ICP18.5 open reading frame in Escherichia coli as a C...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.65.7.3746-3758.1991
更新日期：1991-07-01 00:00:00
abstract：UNLABELLED:The latent HIV reservoir is a major impediment to curing HIV infection. The contribution of CD4(+) T cell activation status to the establishment and maintenance of the latent reservoir was investigated by enumerating viral DNA components in a cohort of 12 individuals commencing antiretroviral therapy (ART) c...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.03331-13
更新日期：2014-03-01 00:00:00
abstract：:The human cytomegalovirus (HCMV) UL138 protein downregulates the cell surface expression of the multidrug resistance-associated protein 1 (MRP1) transporter. We examined the genetic requirements within UL138 for MRP1 downregulation. We determined that the acidic cluster dileucine motif is essential for UL138-mediated ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00430-19
更新日期：2019-05-15 00:00:00
abstract：:Mason-Pfizer monkey virus 60-70S RNA has a molecular weight of 8 times 10-6 when analyzed on polyacrylamide gels. Dissociation of 60-70S RNA of Mason-Pfizer monkey virus and murine leukemia virus by heat or formamide (40%) resulted in conversion to identical subunit structures of 2.8 times 10-6 daltons; treatment with...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.15.2.423-427.1975
更新日期：1975-02-01 00:00:00
abstract：:The iridovirus frog virus 3 (FV3) can replicate in culture in fat head minnow (FHM) fish cells or in BHK-21 hamster cells. Viral DNA replication commences about 3 h after infection of FHM cells with FV3. Between 3 and 6 h postinfection (p.i.), a portion of the intranuclear FV3 DNA is partly unmethylated. At later time...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.67.12.6973-6978.1993
更新日期：1993-12-01 00:00:00
abstract：:The emergence of drug resistance-conferring mutations can severely compromise the success of chemotherapy directed against human immunodeficiency virus type 1 (HIV-1). The M184V and/or L74V mutation in the reverse transcriptase (RT) gene are frequently found in viral isolates from patients treated with the nucleoside ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.77.16.8621-8632.2003
更新日期：2003-08-01 00:00:00
abstract：:Hemagglutinin (HA) of influenza virus must be activated by proteolysis before the virus can become infectious. Previous studies indicated that HA cleavage is driven by membrane-bound or extracellular serine proteases in the respiratory tract. However, there is still uncertainty as to which proteases are critical for a...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00421-17
更新日期：2017-07-27 00:00:00
