Abstract:
:The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential protein. Previous studies have shown that the UL25 gene product is a virion component (M. A. Ali et al., Virology 216:278-283, 1996) involved in virus penetration and capsid assembly (C. Addison et al., Virology 138:246-259, 1984). In this study, we describe the isolation of a UL25 mutant (KUL25NS) that was constructed by insertion of an in-frame stop codon in the UL25 open reading frame and propagated on a complementing cell line. Although the mutant was capable of synthesis of viral DNA, it did not form plaques or produce infectious virus in noncomplementing cells. Antibodies specific for the UL25 protein were used to demonstrate that KUL25NS-infected Vero cells did not express the UL25 protein. Western immunoblotting showed that the UL25 protein was associated with purified, wild-type HSV A, B, and C capsids. Transmission electron microscopy indicated that the nucleus of Vero cells infected with KUL25NS contained large numbers of both A and B capsids but no C capsids. Analysis of infected cells by sucrose gradient sedimentation analysis confirmed that the ratio of A to B capsids was elevated in KUL25NS-infected Vero cells. Following restriction enzyme digestion, specific terminal fragments were observed in DNA isolated from KUL25NS-infected Vero cells, indicating that the UL25 gene was not required for cleavage of replicated viral DNA. The latter result was confirmed by pulsed-field gel electrophoresis (PFGE), which showed the presence of genome-size viral DNA in KUL25NS-infected Vero cells. DNase I treatment prior to PFGE demonstrated that monomeric HSV DNA was not packaged in the absence of the UL25 protein. Our results indicate that the product of the UL25 gene is required for packaging but not cleavage of replicated viral DNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
McNab AR,Desai P,Person S,Roof LL,Thomsen DR,Newcomb WW,Brown JC,Homa FLdoi
10.1128/JVI.72.2.1060-1070.1998subject
Has Abstractpub_date
1998-02-01 00:00:00pages
1060-70issue
2eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.6999-7008.2001
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8609-8614.2004
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4841-4848.1998
更新日期:1998-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02281-09
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1712-1716.1987
更新日期:1987-05-01 00:00:00
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更新日期:2016-11-28 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.70.2.737-744.1996
更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.66.10.5929-5936.1992
更新日期:1992-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.52.3.1036-1038.1984
更新日期:1984-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1188-1193.1989
更新日期:1989-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8234-8242.2000
更新日期:2000-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02904-14
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.329-333.1979
更新日期:1979-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/JVI.00537-07
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.795-806.1980
更新日期:1980-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.3.886-894.1981
更新日期:1981-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5883-5890.1990
更新日期:1990-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01834-18
更新日期:2019-06-14 00:00:00