Expression of polyomavirus large T antigen by using a baculovirus vector.

Abstract:

:A gene encoding the large T antigen of polyomavirus was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus so that gene expression was under the control of the strong, very late polyhedrin gene promoter. Significantly more large T antigen was produced in recombinant virus-infected insect cells than was observed in polyomavirus-transformed mouse cells. The insect-derived T antigen exhibited polyomavirus origin-specific DNA binding. The baculovirus expression system provides a convenient source of T antigen for in vitro studies.

journal_name

J Virol

journal_title

Journal of virology

authors

Rice WC,Lorimer HE,Prives C,Miller LK

doi

10.1128/JVI.61.5.1712-1716.1987

subject

Has Abstract

pub_date

1987-05-01 00:00:00

pages

1712-6

issue

5

eissn

0022-538X

issn

1098-5514

journal_volume

61

pub_type

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