Abstract:
:Cells nonproductively transformed by a variant of the Snyder-Theilen strain of feline sarcoma virus (FeSV) expressed an 85,000-dalton polyprotein (P85) with associated tyrosine-specific protein kinase activity. We identified within this polyprotein a single tyrosine acceptor site for its enzyme activity. This acceptor site, as well as two serine phosphorylation sites localized with the p12 structural component of Snyder-Theilen FeSv P85, was phosphorylated in cells nonproductively transformed by Snyder-Theilen FeSv. In contrast, infection by Snyder-Theilen FeSV transformation-defective mutants resulted in phosphorylation only of the two serine acceptor sites, indicating phosphorylation of the tyrosine acceptor site to be transformation specific. In addition, we describe in vitro labeling conditions, using unfractionated cell extracts, which resulted in preferential phosphorylation of the single Snyder-Theilen FeSV tyrosine-specific acceptor site.
journal_name
J Viroljournal_title
Journal of virologyauthors
Blomberg J,Van de Ven WJ,Reynolds FH Jr,Nalewaik RP,Stephenson JRdoi
10.1128/JVI.38.3.886-894.1981subject
Has Abstractpub_date
1981-06-01 00:00:00pages
886-94issue
3eissn
0022-538Xissn
1098-5514journal_volume
38pub_type
杂志文章abstract::The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters em...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01259-13
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abstract::Alkylation of T7 bacteriophage by methyl methane sulfonate blocked superinfection exclusion. This blockage could be correlated with a delay in the synthesis of phage-specific proteins. Therefore we conclude that protein synthesis directed by the primary infecting phage is required for efficient exclusion of superinfec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.708-710.1982
更新日期:1982-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01717-08
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pub_type: 杂志文章
doi:10.1128/jvi.77.12.6601-6612.2003
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.3.743-749.1985
更新日期:1985-12-01 00:00:00
abstract::Ribonucleoprotein from cells acutely or persistently infected with measles virus were shown to be infectious by the calcium phosphate technique. Very little or no infectivity was obtained when calcium phosphate precipitation was omitted. Electron microscopy showed that the majority of ribonucleoprotein structures isol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.329-333.1979
更新日期:1979-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.8.4268-4277.2004
更新日期:2004-04-01 00:00:00
abstract::The major immediate-early (MIE) transactivator proteins of cytomegaloviruses (CMV) play a pivotal role in the initiation of virus-host cell interactions. Therefore, cis- and trans-acting factors influencing the expression of these proteins through their upstream promoter-enhancer regions are important determinants of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.516-529.1993
更新日期:1993-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-04-14 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8384-8391.1998
更新日期:1998-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6763-6766.1994
更新日期:1994-10-01 00:00:00
abstract::Herpes B virus (BV) naturally infects macaque monkeys and is genetically similar to herpes simplex virus (HSV). Zoonotic infection of humans can cause encephalitis and if untreated has a fatality rate of ∼80%. The frequent use of macaques in biomedical research emphasizes the need to understand the molecular basis of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00593-10
更新日期:2010-09-01 00:00:00
abstract::The roles of the capsid protein (CP) and the CP coding sequence of tobacco etch potyvirus (TEV) in genome amplification were analyzed. A series of frameshift-stop codon mutations that interrupted translation of the CP coding sequence at various positions were introduced into the TEV genome. A series of 3' deletion mut...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4370-4379.1996
更新日期:1996-07-01 00:00:00
abstract::RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to estimate the number of viral RNA abundance classes, their relative concentrations, and the fraction of each DNA strand fro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.2.465-477.1976
更新日期:1976-11-01 00:00:00
abstract::We report sequence hypervariability in the viral protein 1 (VP1) interaction domain of VP2 in the norovirus (NoV) genogroup II genotype 4 (GII.4) lineage on 3 levels: (i) the global evolution of pandemic/epidemic strains from the mid-1970s through post-2006, (ii) the local emergence of an epidemic strain, and (iii) an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00228-11
更新日期:2012-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1998-2005.1999
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abstract::The biologically relevant form of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) glycoprotein is oligomeric, with the major points of contact between oligomeric partners located in the ectodomain of gp41. To identify and map conserved epitopes and regions in gp41 where structure is influenced by quater...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.2674-2684.1997
更新日期:1997-04-01 00:00:00
abstract::Retrovirus infection is normally limited to cells within a specific host range which express a cognate receptor that is recognized by the product of the env gene. We describe retrovirus infection of cells outside of their normal host range when the infection is performed in the presence of a replication-defective aden...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1887-1894.1995
更新日期:1995-03-01 00:00:00
abstract::Linear hepadnavirus DNA in primary hepatocyte cultures efficiently participates in intra- and intermolecular nonhomologous recombination at its ends. The products of this recombination are (i) monomeric covalently closed circular DNAs (cccDNAs) with deletions and insertions around the site of joining and (ii) oligomer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4029-4036.1995
更新日期:1995-07-01 00:00:00
abstract:UNLABELLED:Coronaviruses (CoVs) are unique in encoding a 3'→5' exoribonuclease within nonstructural protein 14 (nsp14-ExoN) that is required for high-fidelity replication, likely via proofreading. nsp14 associates with the CoV RNA-dependent RNA polymerase (nsp12-RdRp), and nsp14-ExoN activity is enhanced by binding nsp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00110-15
更新日期:2015-06-01 00:00:00
abstract::Expression of the region of the feline calicivirus (FCV) ORF1 encoded by nucleotides 3233 to 4054 in an in vitro rabbit reticulocyte system resulted in synthesis of an active proteinase that specifically processes the viral nonstructural polyprotein. Site-directed mutagenesis of the cysteine (Cys1193) residue in the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6626-6633.1999
更新日期:1999-08-01 00:00:00
abstract::The Epstein-Barr virus (EBV) BamHI-A rightward transcripts (BARTs) are expressed in all EBV-associated tumors as well as in latently infected B cells in vivo and cultured B-cell lines. One of the BART family transcripts contains an open reading frame, RPMS1, that encodes a nuclear protein termed RPMS. Reverse transcri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.6.2946-2956.2001
更新日期:2001-03-01 00:00:00
abstract::Bovine herpesvirus 1 (BoHV-1) is an alphaherpesvirus that causes disease in cattle populations worldwide. Sphingomyelin (SM) is the most abundant sphingolipid in the mammalian cell membrane, where it preferentially associates with cholesterol to form lipid raft domains. SM is a substrate for the lysosome-resident enzy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01547-18
更新日期:2019-02-19 00:00:00
abstract::The great majority of viral mRNAs in mouse C127 cells transformed by bovine papillomavirus type 1 (BPV) have a common 3' end at the early polyadenylation site which is 23 nucleotides (nt) downstream of a canonical poly(A) consensus signal. Twenty percent of BPV mRNA from productively infected cells bypasses the early ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7705-7710.1993
更新日期:1993-12-01 00:00:00
abstract::Hypoxia-inducible factor 1 (HIF-1) is a ubiquitously expressed transcriptional regulator involved in induction of numerous genes associated with angiogenesis and tumor growth. Kaposi's sarcoma, associated with increased angiogenesis, is a highly vascularized, endothelial cell-derived tumor. Previously, we have shown t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00611-07
更新日期:2007-10-01 00:00:00
abstract::Herpesvirus saimiri (HVS) is divided into three subgroups, A, B, and C, based on sequence divergence at the left end of genomic DNA in which the saimiri transforming protein (STP) resides. Subgroup A and C strains transform primary common marmoset lymphocytes to interleukin-2-independent growth, whereas subgroup B str...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.8102-8110.2000
更新日期:2000-09-01 00:00:00
abstract::Recent studies of varicella-zoster virus (VZV) DNA sequence variation, involving large numbers of globally distributed clinical isolates, suggest that this virus has diverged into at least three distinct genotypes designated European (E), Japanese (J), and mosaic (M). In the present study, we determined and analyzed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00835-06
更新日期:2006-10-01 00:00:00