Abstract:
:Hepatitis C virus (HCV) contains two membrane-associated envelope glycoproteins, E1 and E2, which assemble as a heterodimer in the endoplasmic reticulum (ER). In this study, predictive algorithms and genetic analyses of deletion mutants and glycosylation site variants of the E1 glycoprotein were used to suggest that the glycoprotein can adopt two topologies in the ER membrane: the conventional type I membrane topology and a polytopic topology in which the protein spans the ER membrane twice with an intervening cytoplasmic loop (amino acid residues 288 to 360). We also demonstrate that the E1 glycoprotein is able to associate with the HCV core protein, but only upon oligomerization of the core protein in the presence of tRNA to form capsid-like structures. Yeast two-hybrid and immunoprecipitation analyses reveal that oligomerization of the core protein is promoted by amino acid residues 72 to 91 in the core. Furthermore, the association between the E1 glycoprotein and the assembled core can be recapitulated using a fusion protein containing the putative cytoplasmic loop of the E1 glycoprotein. This fusion protein is also able to compete with the intact E1 glycoprotein for binding to the core. Mutagenesis of the cytoplasmic loop of E1 was used to define a region of four amino acids (residues 312 to 315) that is important for interaction with the assembled HCV core. Taken together, our studies suggest that interaction between the self-oligomerized HCV core and the E1 glycoprotein is mediated through the cytoplasmic loop present in a polytopic form of the E1 glycoprotein.
journal_name
J Viroljournal_title
Journal of virologyauthors
Nakai K,Okamoto T,Kimura-Someya T,Ishii K,Lim CK,Tani H,Matsuo E,Abe T,Mori Y,Suzuki T,Miyamura T,Nunberg JH,Moriishi K,Matsuura Ydoi
10.1128/JVI.01203-06subject
Has Abstractpub_date
2006-11-01 00:00:00pages
11265-73issue
22eissn
0022-538Xissn
1098-5514pii
JVI.01203-06journal_volume
80pub_type
杂志文章abstract::A vaccine regimen that elicits broadly neutralizing antibodies (bNAbs) is a major goal in HIV-1 vaccine research. In this study, we assessed the immunogenicity of the CAP256 superinfecting viral envelope (CAP256 SU) protein delivered by modified vaccinia virus Ankara (MVA) and DNA vaccines in different prime-boost com...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02155-18
更新日期:2019-04-03 00:00:00
abstract::The role of cellular genes in West Nile virus (WNV) replication is not well understood. Examination of cellular transcripts upregulated during WNV infection revealed an increase in the expression of the src family kinase (SFK) c-Yes. WNV-infected cell lines treated with the SFK inhibitor PP2 demonstrated a 2- to 4-log...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11943-11951.2005
更新日期:2005-09-01 00:00:00
abstract::Synthesis of the fiber protein of human adenovirus serotype 2 (Ad2) is 100-fold lower in abortively infected monkey cells, compared with productive infections, despite only a 5- to 10-fold reduction in fiber mRNA levels. Previously Anderson and Klessig (Proc. Natl. Acad Sci. USA 81:4023-4027, 1984) demonstrated a dire...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.10.4376-4385.1989
更新日期:1989-10-01 00:00:00
abstract::In wild aquatic birds and poultry around the world, influenza A viruses carrying 15 antigenic subtypes of hemagglutinin (HA) and 9 antigenic subtypes of neuraminidase (NA) have been described. Here we describe a previously unidentified antigenic subtype of HA (H16), detected in viruses circulating in black-headed gull...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2814-2822.2005
更新日期:2005-03-01 00:00:00
abstract::2G12 is a broadly neutralizing anti-HIV-1 monoclonal human IgG1 antibody reactive with a high-mannose glycan cluster on the surface of glycoprotein gp120. A key feature of this very highly mutated antibody is domain exchange of the heavy-chain variable region (V(H)) with the V(H) of the adjacent Fab of the same immuno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01111-10
更新日期:2010-10-01 00:00:00
abstract::The simian picornaviruses were isolated from various primate tissues during the development of general tissue culture methods in the 1950s to 1970s or from specimens derived from primates used in biomedical research. Twenty simian picornavirus serotypes are recognized, and all are presently classified within the Enter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1244-1251.2002
更新日期:2002-02-01 00:00:00
abstract::Intact, purified particles of the nodaviruses flock house virus and nodamura virus that were either transfected into cells that were resistant to infection or introduced into in vitro translation systems directed the synthesis of viral proteins. We infer that direct interaction of these nodavirus particles with cytopl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7974-7977.1997
更新日期:1997-10-01 00:00:00
abstract:UNLABELLED:Adeno-associated virus (AAV) has long been known to inhibit helper adenovirus (Ad) replication independently of AAV Rep protein expression. More recently, replication of Ad serotype 5 (Ad5)/AAV serotype 2 (AAV-2) hybrid vectors was shown to be inhibited incisby a sequence near the 3' end of AAVrep, termed th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03120-15
更新日期:2016-03-28 00:00:00
abstract::The nucleotide sequence of the src gene and flanking regions of the Schmidt-Ruppin strain of Rous sarcoma virus (SR-A) was determined. The src region of SR-A was very homologous to that of recovered avian sarcoma virus (rASV1441), with only 17 differences among 1,578 nucleotides. The size of the predicted protein was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.1.12-18.1982
更新日期:1982-10-01 00:00:00
abstract:UNLABELLED:Many viruses have the capacity to prevent a cell from being infected by a second virus, often termed superinfection exclusion. Alphaherpesviruses, including the human pathogen herpes simplex virus 1 (HSV-1) and the animal herpesvirus pseudorabies virus (PRV), encode a membrane-bound glycoprotein, gD, that ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00089-16
更新日期:2016-03-28 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential protein. Previous studies have shown that the UL25 gene product is a virion component (M. A. Ali et al., Virology 216:278-283, 1996) involved in virus penetration and capsid assembly (C. Addison e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1060-1070.1998
更新日期:1998-02-01 00:00:00
abstract::Mouse hepatitis virus (MHV) infection spreads from MHV-infected DBT cells, which express the MHV receptor CEACAM1 (MHVR), to BHK cells, which are devoid of the receptor, by intercellular membrane fusion (MHVR-independent fusion). This mode of infection is a property of wild-type (wt) JHMV cl-2 virus but is not seen in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.950-958.2002
更新日期:2002-02-01 00:00:00
abstract::ts5, a temperature-sensitive mutant of influenza B virus, belongs to one of seven recombination groups. When the mutant infected MDCK cells at the nonpermissive temperature (37.5 degrees C), infectious virus was produced at very low levels compared with the yield at the permissive temperature (32 degrees C) and hemagg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3264-3273.1993
更新日期:1993-06-01 00:00:00
abstract:UNLABELLED:Oncolytic viruses (OVs) are attractive avenues of cancer therapy due to the absence of toxic side effects often seen with current treatment modalities. Bovine herpesvirus 1 (BHV-1) is a species-specific virus that does not induce cytotoxicity in normal primary human cells but can infect and kill various huma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00849-14
更新日期:2014-06-01 00:00:00
abstract::The protein (VPg) linked to the 5' termini of poliovirus RNAs resolved into two species when subjected to non-equilibrium electrofocusing. The differently charged forms of VPg were not due to protein phosphorylation nor to variability of the number of phosphate residues associated with the nucleotide moiety remaining ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.3.863-871.1981
更新日期:1981-06-01 00:00:00
abstract::Mutagenesis of the transmembrane domain and cytoplasmic tail of human immunodeficiency virus type 1 envelope glycoprotein gp160 revealed that its intracellular transport and processing in transfected cell lines were modulated by a functional domain included in the carboxy-terminal sequence consisting of residues 751 t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3100-3103.1990
更新日期:1990-06-01 00:00:00
abstract::A significant percentage of the population latently harbors Epstein-Barr virus (EBV) in B cells. One EBV-encoded protein, latent membrane protein 2A (LMP2A), is expressed in tissue culture models of EBV latent infection, in human infections, and in many of the EBV-associated proliferative disorders. LMP2A constitutive...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7355-7362.2005
更新日期:2005-06-01 00:00:00
abstract::To investigate the hypothesis that neurological sequelae are associated with persistent West Nile virus (WNV) and neuropathology, we developed an electrophysiological motor unit number estimation (MUNE) assay to measure the health of motor neurons temporally in hamsters. The MUNE assay was successful in identifying ch...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00017-09
更新日期:2009-05-01 00:00:00
abstract:UNLABELLED:Recent experiments suggest that some glycoprotein (GP)-specific monoclonal antibodies (MAbs) can protect experimental animals against the filovirus Ebola virus (EBOV). There is a need for isolation of MAbs capable of neutralizing multiple filoviruses. Antibody neutralization assays for filoviruses frequently...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00101-16
更新日期:2016-03-28 00:00:00
abstract::The initial host response to viral infection occurs after Toll-like receptors (TLRs) on dendritic cells (DC) are stimulated by viral nucleic acids (double-stranded RNA, single-stranded RNA) and alpha interferon (IFN-alpha) and IFN-beta are produced. We hypothesized that pharmacologic induction of innate antiviral resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14355-14370.2005
更新日期:2005-11-01 00:00:00
abstract::Hantaviruses are tripartite negative-sense RNA viruses and members of the Bunyaviridae family. The nucleocapsid (N) protein is encoded by the smallest of the three genome segments (S). N protein is the principal structural component of the viral capsid and is central to the hantavirus replication cycle. We examined in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.8281-8288.2004
更新日期:2004-08-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is consistently found in Kaposi's sarcoma lesions and in body-cavity-based lymphomas. A 17-kb KSHV lambda clone was obtained directly from a Kaposi's sarcoma lesion. DNA sequence analysis of this clone identified an open reading frame whi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1984-1991.1997
更新日期:1997-03-01 00:00:00
abstract::We have devised an enrichment scheme for the isolation of export-competent derivatives of pseudorabies virus glycoprotein gIII signal peptide mutants. Enrichment is based upon a growth advantage imparted upon gIII-containing virions compared with virions lacking the glycoprotein. Each of identified derivatives suppres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.2787-2794.1994
更新日期:1994-05-01 00:00:00
abstract::After uptake into target cells, many nonenveloped viruses undergo conformational changes in the low-pH environment of the endocytic compartment. This results in exposure of amphipathic viral peptides and/or hydrophobic protein domains that are inserted into and either disrupt or perforate the vesicular membranes. The ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00329-11
更新日期:2011-08-01 00:00:00
abstract::The relationship between immunosenescence and the host response to virus infection is poorly understood at the molecular level. Two different patterns of pulmonary host responses to virus were observed when gene expression profiles from severe acute respiratory syndrome coronavirus (SARS-CoV)-infected young mice that ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00489-08
更新日期:2008-10-01 00:00:00
abstract::The family Geminiviridae comprises seven genera differentiated by genome organization, sequence similarity, and insect vector. Capulavirus, an eighth genus, has been proposed to accommodate two newly discovered highly divergent geminiviruses that presently have no known vector. Alfalfa leaf curl virus, identified here...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00453-15
更新日期:2015-09-01 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) infection in the central nervous system (CNS) is characterized by replication in macrophages or brain microglia that express low levels of the CD4 receptor and is the cause of HIV-associated dementia and related cognitive and motor disorders that affect 20 to 30% o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01237-14
更新日期:2014-08-01 00:00:00
abstract::Human cytomegalovirus (HCMV) pathogenesis is dependent on the hematogenous spread of the virus to host tissue. While data suggest that infected monocytes are required for viral dissemination from the blood to the host organs, infected endothelial cells are also thought to contribute to this key step in viral pathogene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01016-06
更新日期:2006-12-01 00:00:00
abstract::After isolation, the DNA of simian virus 40 appeared as a negative supertwist (form I) or as an open circle with at least one single-strand scission (form II). Under the denaturation conditions usually applied, such as heating in the presence of formaldehyde or application of alkali, form I molecules could appear as "...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.585-598.1975
更新日期:1975-03-01 00:00:00
abstract::Sulfation is a posttranslational modification of proteins which occurs on either the tyrosine residues or the carbohydrate moieties of some glycoproteins. In the case of secretory proteins, sulfation has been hypothesized to act as a signal for export from the cell. We have shown that the human immunodeficiency virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.6953-6959.1992
更新日期:1992-12-01 00:00:00