Trimeric hantavirus nucleocapsid protein binds specifically to the viral RNA panhandle.


:Hantaviruses are tripartite negative-sense RNA viruses and members of the Bunyaviridae family. The nucleocapsid (N) protein is encoded by the smallest of the three genome segments (S). N protein is the principal structural component of the viral capsid and is central to the hantavirus replication cycle. We examined intermolecular N-protein interaction and RNA binding by using bacterially expressed Sin Nombre virus N protein. N assembles into di- and trimeric forms. The mono- and dimeric forms exist transiently and assemble into a trimeric form. In contrast, the trimer is highly stable and does not efficiently disassemble into the mono- and dimeric forms. The purified N-protein trimer is able to discriminate between viral and nonviral RNA molecules and, interestingly, recognizes and binds with high affinity the panhandle structure composed of the 3' and 5' ends of the genomic RNA. In contrast, the mono- and dimeric forms of N bind RNA to form a complex that is semispecific and salt sensitive. We suggest that trimerization of N protein is a molecular switch to generate a protein complex that can discriminate between viral and nonviral RNA molecules during the early steps of the encapsidation process.


J Virol


Journal of virology


Mir MA,Panganiban AT




Has Abstract


2004-08-01 00:00:00














  • A three-helix junction is the interface between two functional domains of prohead RNA in 29 DNA packaging.

    abstract::The double-stranded-DNA bacteriophages employ powerful molecular motors to translocate genomic DNA into preformed capsids during the packaging step in phage assembly. Bacillus subtilis bacteriophage 29 has an oligomeric prohead RNA (pRNA) that is an essential component of its packaging motor. The crystal structure of ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Zhao W,Saha M,Ke A,Morais MC,Jardine PJ,Grimes S

    更新日期:2012-11-01 00:00:00

  • Activity increase in respiratory chain complexes by rubella virus with marginal induction of oxidative stress.

    abstract::Mitochondria are important for the viral life cycle, mainly by providing the energy required for viral replication and assembly. A highly complex interaction with mitochondria is exerted by rubella virus (RV), which includes an increase in the mitochondrial membrane potential as a general marker for mitochondrial acti...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Claus C,Schönefeld K,Hübner D,Chey S,Reibetanz U,Liebert UG

    更新日期:2013-08-01 00:00:00

  • Expanded tropism and altered activation of a retroviral glycoprotein resistant to an entry inhibitor peptide.

    abstract::The envelope of class I viruses can be a target for potent viral inhibitors, such as the human immunodeficiency virus type 1 (HIV-1) inhibitor enfuvirtide, which are derived from the C-terminal heptad repeat (HR2) of the transmembrane (TM) subunit. Resistance to an HR2-based peptide inhibitor of a model retrovirus, su...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Amberg SM,Netter RC,Simmons G,Bates P

    更新日期:2006-01-01 00:00:00

  • Scavenger receptor BI and BII expression levels modulate hepatitis C virus infectivity.

    abstract::Hepatitis C virus (HCV) enters cells via a pH- and clathrin-dependent endocytic pathway. Scavenger receptor BI (SR-BI) and CD81 are important entry factors for HCV internalization into target cells. The SR-BI gene gives rise to at least two mRNA splice variants, SR-BI and SR-BII, which differ in their C termini. SR-BI...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Grove J,Huby T,Stamataki Z,Vanwolleghem T,Meuleman P,Farquhar M,Schwarz A,Moreau M,Owen JS,Leroux-Roels G,Balfe P,McKeating JA

    更新日期:2007-04-01 00:00:00

  • Effects of an epitope-specific CD8+ T-cell response on murine coronavirus central nervous system disease: protection from virus replication and antigen spread and selection of epitope escape mutants.

    abstract::Both CD4(+) and CD8(+) T cells are required for clearance of the murine coronavirus mouse hepatitis virus (MHV) during acute infection. We investigated the effects of an epitope-specific CD8(+) T-cell response on acute infection of MHV, strain A59, in the murine CNS. Mice with CD8(+) T cells specific for gp33-41 (an H...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Chua MM,MacNamara KC,San Mateo L,Shen H,Weiss SR

    更新日期:2004-02-01 00:00:00

  • Respiratory syncytial virus (RSV) G glycoprotein is not necessary for vaccine-enhanced disease induced by immunization with formalin-inactivated RSV.

    abstract::Following respiratory syncytial virus (RSV) challenge, mice immunized with RSV G or with formalin-inactivated RSV (FI-RSV) exhibit severe disease associated with type 2 cytokine production and pulmonary eosinophilia. This has led to the proposal that the presence of RSV G is the factor in FI-RSV that induces disease-e...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Johnson TR,Teng MN,Collins PL,Graham BS

    更新日期:2004-06-01 00:00:00

  • Basis for variable response of arboviruses to guanidine treatment.

    abstract::The effect of guanidine on the replication of the group A arboviruses, Sindbis virus, and Semliki Forest virus (SFV) was studied. Guanidine rapidly, but reversibly, inhibited SFV ribonucleic acid (RNA) synthesis. The synthesis of all species of viral RNA was inhibited, but that of ribonuclease-resistant forms was leas...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Friedman RM

    更新日期:1970-11-01 00:00:00

  • Merkel cell polyomavirus-infected Merkel cell carcinoma cells require expression of viral T antigens.

    abstract::Merkel cell carcinoma (MCC) is the most aggressive skin cancer. Recently, it was demonstrated that human Merkel cell polyomavirus (MCV) is clonally integrated in approximately 80% of MCC tumors. However, direct evidence for whether oncogenic viral proteins are needed for the maintenance of MCC cells is still missing. ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Houben R,Shuda M,Weinkam R,Schrama D,Feng H,Chang Y,Moore PS,Becker JC

    更新日期:2010-07-01 00:00:00

  • Viral DNA-Dependent Induction of Innate Immune Response to Hepatitis B Virus in Immortalized Mouse Hepatocytes.

    abstract:UNLABELLED:Hepatitis B virus (HBV) infects hundreds of millions of people worldwide and causes acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HBV is an enveloped virus with a relaxed circular (RC) DNA genome. In the nuclei of infected human hepatocytes, conversion of RC DNA from the incoming viri...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Cui X,Clark DN,Liu K,Xu XD,Guo JT,Hu J

    更新日期:2015-10-21 00:00:00

  • Expression of the Autographa californica nuclear polyhedrosis virus apoptotic suppressor gene p35 in nonpermissive Spodoptera littoralis cells.

    abstract::Apoptosis was postulated as the main barrier to replication of the Autographa californica nuclear polyhedrosis virus (AcMNPV) in a Spodoptera littoralis SL2 cell line (N. Chejanovsky and E. Gershburg, Virology 209:519-525, 1995). Thus, we hypothesized that the viral apoptotic suppressor gene p35 is either poorly expre...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gershburg E,Rivkin H,Chejanovsky N

    更新日期:1997-10-01 00:00:00

  • Exon 3 of the human cytomegalovirus major immediate-early region is required for efficient viral gene expression and for cellular cyclin modulation.

    abstract::The human cytomegalovirus (HCMV) major immediate-early (IE) proteins share an 85-amino-acid N-terminal domain specified by exons 2 and 3 of the major IE region, UL122-123. We have constructed IE Delta30-77, a recombinant virus that lacks the majority of IE exon 3 and consequently expresses smaller forms of both IE1 72...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: White EA,Spector DH

    更新日期:2005-06-01 00:00:00

  • Targeting Innate Immunity for Antiviral Therapy through Small Molecule Agonists of the RLR Pathway.

    abstract:UNLABELLED:The cellular response to virus infection is initiated when pathogen recognition receptors (PRR) engage viral pathogen-associated molecular patterns (PAMPs). This process results in induction of downstream signaling pathways that activate the transcription factor interferon regulatory factor 3 (IRF3). IRF3 pl...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pattabhi S,Wilkins CR,Dong R,Knoll ML,Posakony J,Kaiser S,Mire CE,Wang ML,Ireton RC,Geisbert TW,Bedard KM,Iadonato SP,Loo YM,Gale M Jr

    更新日期:2015-12-16 00:00:00

  • Atypical RNA Elements Modulate Translational Readthrough in Tobacco Necrosis Virus D.

    abstract::Tobacco necrosis virus, strain D (TNV-D), is a positive-strand RNA virus in the genus Betanecrovirus and family Tombusviridae The production of its RNA-dependent RNA polymerase, p82, is achieved by translational readthrough. This process is stimulated by an RNA structure that is positioned immediately downstream of th...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Newburn LR,White KA

    更新日期:2017-03-29 00:00:00

  • Analysis of natural variants of the human immunodeficiency virus type 1 gag-pol frameshift stem-loop structure.

    abstract::Human immunodeficiency virus type 1 uses ribosomal frameshifting for translation of the Gag-Pol polyprotein. Frameshift activities are thought to be tightly regulated. Analysis of gag p1 sequences from 270 plasma virions identified in 64% of the samples the occurrence of polymorphism that could lead to changes in ther...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Telenti A,Martinez R,Munoz M,Bleiber G,Greub G,Sanglard D,Peters S

    更新日期:2002-08-01 00:00:00

  • A highly divergent picornavirus in a marine mammal.

    abstract::Nucleic acids from an unidentified virus from ringed seals (Phoca hispida) were amplified using sequence-independent PCR, subcloned, and then sequenced. The full genome of a novel RNA virus was derived, identifying the first sequence-confirmed picornavirus in a marine mammal. The phylogenetic position of the tentative...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kapoor A,Victoria J,Simmonds P,Wang C,Shafer RW,Nims R,Nielsen O,Delwart E

    更新日期:2008-01-01 00:00:00

  • A heterologous heparin-binding domain can promote functional attachment of a pseudorabies virus gC mutant to cell surfaces.

    abstract::The efficient attachment of pseudorabies virus to cultured cells is dependent on an electrostatic interaction between negatively charged cell surface heparan sulfate and the viral envelope glycoprotein gC. Deletion of the first one-third of gC severely impairs virus attachment, but the mutant virions are still capable...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Flynn SJ,Ryan P

    更新日期:1995-02-01 00:00:00

  • Porcine teschoviruses comprise at least eleven distinct serotypes: molecular and evolutionary aspects.

    abstract::Nucleotide sequencing and phylogenetic analysis of 10 recognized prototype strains of the porcine enterovirus (PEV) cytopathic effect (CPE) group I reveals a close relationship of the viral genomes to the previously sequenced strain F65, supporting the concept of a reclassification of this virus group into a new picor...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Zell R,Dauber M,Krumbholz A,Henke A,Birch-Hirschfeld E,Stelzner A,Prager D,Wurm R

    更新日期:2001-02-01 00:00:00

  • A bulged region of the hepatitis B virus RNA encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesis.

    abstract::Human hepatitis B virus (HBV) is a small DNA virus that replicates inside the viral nucleocapsid by reverse transcription of an RNA intermediate. Encapsidation of this RNA pregenome is mediated by the interaction of the viral replication enzyme P with the structured 5'-proximal RNA element epsilon; replication was tho...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Nassal M,Rieger A

    更新日期:1996-05-01 00:00:00

  • Induction of protective immunity against Japanese encephalitis in mice by immunization with a plasmid encoding Japanese encephalitis virus premembrane and envelope genes.

    abstract::A DNA vaccine plasmid containing the Japanese encephalitis (JE) virus premembrane (prM) and envelope (E) genes (designated pcDNA3JEME) was evaluated for immunogenicity and protective efficacy in mice. Two immunizations of 4-week-old female ICR mice with pcDNA3JEME by intramuscular or intradermal injections at a dose o...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Konishi E,Yamaoka M,Khin-Sane-Win,Kurane I,Mason PW

    更新日期:1998-06-01 00:00:00

  • Adeno-associated virus type 5 utilizes alternative translation initiation to encode a small Rep40-like protein.

    abstract::Alternative splicing of adeno-associated virus type 2 (AAV2) P19-generated pre-mRNAs generates the small Rep proteins Rep52 and Rep40, which differ in their carboxyl termini. Both proteins are required for optimal packaging of AAV2 genomes. AAV5 Rep-encoding P19-generated transcripts are primarily polyadenylated withi...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Farris KD,Pintel DJ

    更新日期:2010-01-01 00:00:00

  • Tetherin Sensitivity of Influenza A Viruses Is Strain Specific: Role of Hemagglutinin and Neuraminidase.

    abstract:UNLABELLED:The expression of the antiviral host cell factor tetherin is induced by interferon and can inhibit the release of enveloped viruses from infected cells. The Vpu protein of HIV-1 antagonizes the antiviral activity of tetherin, and tetherin antagonists with Vpu-like activity have been identified in other virus...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gnirß K,Zmora P,Blazejewska P,Winkler M,Lins A,Nehlmeier I,Gärtner S,Moldenhauer AS,Hofmann-Winkler H,Wolff T,Schindler M,Pöhlmann S

    更新日期:2015-09-01 00:00:00

  • Oligomer formation of the gB glycoprotein of herpes simplex virus type 1.

    abstract::Oligomer formation of the gB glycoprotein of herpes simplex virus type 1 was studied by sedimentation analysis of radioactively labeled infected cell and virion lysates. Fractions from sucrose gradients were precipitated with a pool of gB-specific monoclonal antibodies and analyzed by sodium dodecyl sulfate-polyacryla...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Highlander SL,Goins WF,Person S,Holland TC,Levine M,Glorioso JC

    更新日期:1991-08-01 00:00:00

  • In Vivo Knockdown of the Herpes Simplex Virus 1 Latency-Associated Transcript Reduces Reactivation from Latency.

    abstract::During herpes simplex virus (HSV) latency, most viral genes are silenced, with the exception of one region of the genome encoding the latency-associated transcript (LAT). This long noncoding RNA was originally described as having a role in enhancing HSV-1 reactivation. However, subsequent evidence showing that the LAT...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Watson ZL,Washington SD,Phelan DM,Lewin AS,Tuli SS,Schultz GS,Neumann DM,Bloom DC

    更新日期:2018-07-31 00:00:00

  • Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses.

    abstract::This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells. Good clean-out of infectivity from rhinovirus harvests was obtained with the continuous-flow B-V and B-IX rotors. Use of the B-V rotor resulted in the successful concentration of rhinov...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gerin JL,Richter WR,Fenters JD,Holper JC

    更新日期:1968-09-01 00:00:00

  • Three arginine residues within the RGG box are crucial for ICP27 binding to herpes simplex virus 1 GC-rich sequences and for efficient viral RNA export.

    abstract::ICP27 is a multifunctional protein that is required for herpes simplex virus 1 mRNA export. ICP27 interacts with the mRNA export receptor TAP/NXF1 and binds RNA through an RGG box motif. Unlike other RGG box proteins, ICP27 does not bind G-quartet structures but instead binds GC-rich sequences that are flexible in str...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Corbin-Lickfett KA,Souki SK,Cocco MJ,Sandri-Goldin RM

    更新日期:2010-07-01 00:00:00

  • Induction and biological properties of defective interfering particles of rabies virus.

    abstract::A method for obtaining large quantities of defective interfering (DI) rabies virus particles that fulfill all the criteria delineated by Huang and Baltimore (1970) is described. The purified rabies DI virion was found to be much shorter (60 to 80 nm) than the complete virion (180 nm) and to have a viral genome of abou...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wiktor TJ,Dietzschold B,Leamnson RN,Koprowski H

    更新日期:1977-02-01 00:00:00

  • Epigenetic regulation of the latency-associated region of Marek's disease virus in tumor-derived T-cell lines and primary lymphoma.

    abstract::Meq is the major Marek's disease virus (MDV)-encoded oncoprotein and is essential for T-cell lymphomagenesis. Meq and several noncoding RNAs, including three microRNA (MiR) clusters, are expressed from the repeats of the MDV genome during latent infection of T cells. To investigate the state of the chromatin in this a...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Brown AC,Nair V,Allday MJ

    更新日期:2012-02-01 00:00:00

  • Identification of a receptor-binding pocket on the envelope protein of friend murine leukemia virus.

    abstract::Based on previous structural and functional studies, a potential receptor-binding site composed of residues that form a pocket at one end of the two long antiparallel helices in the receptor-binding domain of Friend 57 murine leukemia virus envelope protein (RBD) has been proposed. To test this hypothesis, directed su...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Davey RA,Zuo Y,Cunningham JM

    更新日期:1999-05-01 00:00:00

  • Proteomic characterization of pseudorabies virus extracellular virions.

    abstract::Pseudorabies virus (PRV), a member of the Alphaherpesvirinae, has a complex multilayered extracellular virion that is structurally conserved among other herpesviruses. PRV virions contain a double-stranded DNA genome within a proteinaceous capsid surrounded by the tegument, a layer of viral and cellular proteins. The ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kramer T,Greco TM,Enquist LW,Cristea IM

    更新日期:2011-07-01 00:00:00

  • Sequence, function, and regulation of the Vmw65 gene of herpes simplex virus type 2.

    abstract::We determined the sequence of the gene for the virion transactivator protein Vmw65 of herpes simplex virus type 2 (HSV-2), strain 333. An analysis of the coding sequence revealed an overall high degree of primary sequence conservation (86%) relative to the HSV-1 protein, although the carboxy-terminal region which enco...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Greaves RF,O'Hare P

    更新日期:1991-12-01 00:00:00