Abstract:
:The major human tRNA(Lys) isoacceptors, and, are selectively packaged into human immunodeficiency virus type 1 (HIV-1) during assembly, where acts as a primer for reverse transcription. Lysyl-tRNA synthetase (LysRS) is also incorporated into HIV-1, independently of tRNA(Lys), via its interaction with Gag, and is a strong candidate for being the signal that specifically targets tRNA(Lys) for viral incorporation. We have transfected 293T cells with HIV-1 proviral DNA and short interfering RNA (siRNA) specific for LysRS to study the effect of diminished cellular LysRS upon tRNA(Lys) packaging, annealing to viral genomic RNA, and viral production and infectivity. At early time points after siRNA transfection, an 80% inhibition of LysRS incorporation into viruses reflects an 80% reduction of newly synthesized LysRS, rather than a more limited 20 to 25% decrease in the concentration of total cell LysRS, indicating that newly synthesized LysRS in the cell may be the main source of viral LysRS. Viruses produced from cells transfected with siRNA show reduced tRNA(Lys) packaging, reduced annealing to viral RNA, and reduced viral infectivity.
journal_name
J Viroljournal_title
Journal of virologyauthors
Guo F,Cen S,Niu M,Javanbakht H,Kleiman Ldoi
10.1128/jvi.77.18.9817-9822.2003subject
Has Abstractpub_date
2003-09-01 00:00:00pages
9817-22issue
18eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::Varicella-zoster virus (VZV) causes varicella and establishes latency in sensory nerve ganglia, but the characteristics of VZV latency are not well defined. Immunohistochemical detection of the VZV immediate-early 63 (IE63) protein in ganglion neurons has been described, but there are significant discrepancies in esti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02416-09
更新日期:2010-04-01 00:00:00
abstract::The phenotypic defects of two mutants of vaccinia virus, the lesions of which map to gene G2R, were characterized in vivo, and the results suggest a role for the G2R protein in viral transcription elongation. Both a temperature-sensitive mutant, Cts56, and an isatin-beta-thiosemicarbazone-dependent deletion mutant, G2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.47-54.1996
更新日期:1996-01-01 00:00:00
abstract::Epstein-Barr virus (EBV)-associated membrane antigens have been purified from the plasma membranes of the producer cell line P3HR-1 NONO. The antigens were assayed with a specific rabbit anti-ebv antiserum using an 125I-labeled staphylococcal protein A binding assay. The antigens have been shown to be present on purif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.2.458-467.1979
更新日期:1979-11-01 00:00:00
abstract::Monoclonal antibodies (MAbs) directed against epitopes in the V2 domain of human immunodeficiency virus type 1 gp120 often possess neutralizing activity, but these generally are highly type specific, neutralize only laboratory isolates, or have low potency. The most potent of these is C108g, directed against a type-sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.6909-6917.2005
更新日期:2005-06-01 00:00:00
abstract::Generating durable humoral immunity through vaccination depends upon effective interactions of follicular helper T (Tfh) cells with germinal center (GC) B cells. Th1 polarization of Tfh cells is an important process shaping the success of Tfh-GC B cell interactions by influencing costimulatory and cytokine-dependent T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01737-19
更新日期:2020-02-28 00:00:00
abstract:UNLABELLED:Adeno-associated virus (AAV) has long been known to inhibit helper adenovirus (Ad) replication independently of AAV Rep protein expression. More recently, replication of Ad serotype 5 (Ad5)/AAV serotype 2 (AAV-2) hybrid vectors was shown to be inhibited incisby a sequence near the 3' end of AAVrep, termed th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03120-15
更新日期:2016-03-28 00:00:00
abstract::The small envelope protein (E) plays a role of central importance in the assembly of coronaviruses. This was initially established by studies demonstrating that cellular expression of only E protein and the membrane protein (M) was necessary and sufficient for the generation and release of virus-like particles. To inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01577-06
更新日期:2007-03-01 00:00:00
abstract::We previously conducted a phase I/II study using arterial infusions of ONYX-015 (dl1520), a replication-selective adenoviral vector, with E1b deleted, for patients with metastatic colorectal cancer. No dose-limiting toxicities occurred, but >90% of the patients experienced fever. The effects of temperature on the repl...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.79.1.581-591.2005
更新日期:2005-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6710-6713.2001
更新日期:2001-07-01 00:00:00
abstract::A region in addition to and outside the long terminal repeats (LTRs) in the gag gene of the Prague A strain of Rous sarcoma virus was found to be essential in cis for efficient cell transformation by cloned viral DNA. Transformation in chicken embryo fibroblasts, which requires infectious virus production and reinfect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.11.3401-3409.1987
更新日期:1987-11-01 00:00:00
abstract::Feline panleukopenia virus (FPV) and its host range variant, canine parvovirus (CPV), can bind the feline transferrin receptor (TfR), while only CPV binds to the canine TfR. Introducing two CPV-specific changes into FPV (at VP2 residues 93 and 323) endowed that virus with the canine TfR binding property and allowed ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10099-10105.2003
更新日期:2003-09-01 00:00:00
abstract::Human adenovirus 41 (HAdV-41) causes acute gastroenteritis in young children. The main characteristics of HAdV-41 infection are diarrhea and vomiting. Nevertheless, the precise mechanism of HAdV-41-induced diarrhea is unknown, as a suitable small-animal model has not been described. In this study, we used the human mi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00026-18
更新日期:2018-03-14 00:00:00
abstract::The parechoviruses differ in many biological properties from other picornaviruses, and their replication strategy is largely unknown. In order to identify the viral RNA replication complex in human parechovirus type 1 (HPEV-1)-infected cells, we located viral protein and RNA in correlation to virus-induced membrane al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8512-8523.2003
更新日期:2003-08-01 00:00:00
abstract::Precursor polyproteins containing translational products of the gag gene of Moloney murine leukemia virus were purified by gel electrophoresis and cleaved into large fragments by hydroxylamine, mild acid hydrolysis, or cyanogen bromide. The hydroxylamine cleavage method (specific for asparagine-glycine bonds) was adap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.841-859.1983
更新日期:1983-06-01 00:00:00
abstract::The regulation of murine cytomegalovirus early (E) gene expression was studied in the cell line B25, which is stably transfected with the immediate-early ie1/ie3 gene complex. Infection of B25 cells in the presence of the protein synthesis inhibitor cycloheximide resulted in the expression of some E genes, whereas for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.1907-1919.1990
更新日期:1990-05-01 00:00:00
abstract::The herpes simplex virus 1 (HSV-1) multifunctional regulatory protein ICP27 shuttles between the nucleus and cytoplasm in its role as a viral mRNA export factor. Arginine methylation on glycine- and arginine-rich motifs has been shown to regulate protein export. ICP27 contains an RGG box and has been shown to be methy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00238-09
更新日期:2009-06-01 00:00:00
abstract::In adenovirus-infected cells, the epidermal growth factor receptor (EGF-R) is internalized from the cell surface via endosomes and is degraded, and the E3 10,400-dalton protein (10.4K protein) is required for this effect (C. R. Carlin, A. E. Tollefson, H. A. Brady, B. L. Hoffman, and W. S. M. Wold, Cell 57:135-144, 19...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3095-3105.1991
更新日期:1991-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00316-11
更新日期:2011-09-01 00:00:00
abstract::Although hepatitis A virus (HAV) is typically transmitted by the fecal-oral route, little is known of its interactions with cells of the gastrointestinal tract. We studied the replication of HAV in polarized cultures of Caco-2 cells, a human cell line which retains many differentiated functions of small intestinal epi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6476-6484.2000
更新日期:2000-07-01 00:00:00
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abstract::Competitive quantitative PCR and reverse transcriptase-PCR were used to quantitate DNA and RNA from an attenuated ribonucleotide reductase-deleted herpes simplex virus type 1 (HSV-1) mutant in the rat trigeminal ganglion after peripheral inoculation following corneal scarification. Amplification of ganglionic DNA with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7083-7091.1994
更新日期:1994-11-01 00:00:00
abstract::The adenovirus major late promoter (MLP) is active during both the early and late phases of infection. During the early phase the activity of the MLP is similar to those of the other early viral promoters, but during the late phase the rate of transcription from the MLP becomes much greater by comparison. We report he...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.51-60.1990
更新日期:1990-01-01 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) is a cytoplasmic receptor/transcription factor that modulates several cellular and immunological processes following activation by pathogen-associated stimuli, though its role during virus infection is largely unknown. Here, we show that AhR is activated in cells infected with mouse...
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pub_type: 杂志文章
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更新日期:2020-01-17 00:00:00
abstract::The murine polyomavirus (Py) enters mouse fibroblasts and kidney epithelial cells via an endocytic pathway that is caveola-independent (as well as clathrin-independent). In contrast, uptake of simian virus 40 into the same cells is dependent on caveola. Following the initial uptake of Py, both microtubules and microfi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2615-2622.2003
更新日期:2003-02-01 00:00:00
abstract::The recently discovered hepatitis G virus (HGV) or GB virus C (GBV-C) is widely distributed in human populations, and homologues such as HGV/GBV-CCPZ and GBV-A are found in a variety of different primate species. Both epidemiological and phylogenetic analyses support the hypothesis that GB viruses coevolved with their...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5787-5794.1999
更新日期:1999-07-01 00:00:00
abstract::Foot-and-mouth disease virus (FMDV), a member of the Picornaviridae, is a pathogen of cloven-hoofed animals and causes a disease of major economic importance. Picornavirus-infected cells show changes in cell morphology and rearrangement of cytoplasmic membranes, which are a consequence of virus replication. We show he...
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pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
abstract::A consensus binding site for the human papillomavirus (HPV) E2 protein was determined from an unbiased set of degenerate oligonucleotides using cyclic amplification and selection of targets (CASTing). Detectable DNA-protein complexes were formed after six to nine cycles of CASTing. A population of selected binding sit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1722-1735.2001
更新日期:2001-02-01 00:00:00
abstract::Empty capsids of foot-and-mouth disease virus (FMDV) type A22 Iraq 24/64, whose structure has been solved by X-ray crystallography, are unusual for picornaviruses since they contain VP2 and VP4, the cleavage products of the protein precursor VP0. Both the N terminus of VP1 and the C terminus of VP4, which pack togethe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9743-9752.1997
更新日期:1997-12-01 00:00:00
abstract::To replicate its segmented, double-stranded RNA (dsRNA) genome, the rotavirus RNA-dependent RNA polymerase, VP1, must recognize viral plus-strand RNAs (+RNAs) and guide them into the catalytic center. VP1 binds to the conserved 3' end of rotavirus +RNAs via both sequence-dependent and sequence-independent contacts. Se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01689-10
更新日期:2011-03-01 00:00:00
abstract::Infection of receptor-bearing cells by coronaviruses is mediated by their spike (S) proteins. The coronavirus (SARS-CoV) that causes severe acute respiratory syndrome (SARS) infects cells expressing the receptor angiotensin-converting enzyme 2 (ACE2). Here we show that codon optimization of the SARS-CoV S-protein gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10628-10635.2004
更新日期:2004-10-01 00:00:00