Abstract:
:The phenotypic defects of two mutants of vaccinia virus, the lesions of which map to gene G2R, were characterized in vivo, and the results suggest a role for the G2R protein in viral transcription elongation. Both a temperature-sensitive mutant, Cts56, and an isatin-beta-thiosemicarbazone-dependent deletion mutant, G2A, in gene G2R have a characteristic and unique defect in late viral gene expression. The G2R mutants synthesize early viral RNA, early viral proteins, and viral DNA normally under nonpermissive conditions. In G2R mutants, late viral protein synthesis begins at the normal time, low-molecular-weight viral proteins are synthesized in normal quantities, but synthesis of high-molecular-weight viral proteins is reduced in amount. Intermediate and late promoter utilization is normal in G2R mutants, but intermediate and late RNAs are reduced in size. The reduction in length of the intermediate and late mRNAs represents a truncation of mRNA 3' ends. Thus, intermediate and late RNAs are too short to encode large proteins but long enough to encode small proteins, therefore accounting for the protein synthesis phenotype. These results suggest that the G2R protein acts to regulate the elongation potential of the viral RNA polymerase late during a vaccinia virus infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Black EP,Condit RCdoi
10.1128/JVI.70.1.47-54.1996subject
Has Abstractpub_date
1996-01-01 00:00:00pages
47-54issue
1eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::The HTLV-1 singly spliced open reading frame I protein, p12(I), is highly unstable and appears to be necessary for persistent infection in rabbits. Here we demonstrate that p12(I) forms dimers through two putative leucine zipper domains and that its stability is augmented by specific proteasome inhibitors. p12(I) is u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6460-6467.1999
更新日期:1999-08-01 00:00:00
abstract::Enveloped virus vectors are used in a wide variety of applications. We have discovered that treatment of cultured cells with phosphatidylserine (PS) liposomes can increase virus vector infection by up to 20-fold. This effect does not abrogate virus receptor requirements, is specific to PS compared to other phospholipi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11496-11500.2005
更新日期:2005-09-01 00:00:00
abstract::A ribonucleoprotein particle containing about 20% ribonucleic acid (RNA), and containing little if any phospholipid or glucosamine, was recovered in high yield after treatment of Schmidt-Ruppin strain of Rous sarcoma virus and B77 virus with the nonionic detergent Nonidet P-40. This structure, which probably derives f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.5.1010-1020.1972
更新日期:1972-11-01 00:00:00
abstract::The induction of an interferon-induced antiviral state is a powerful cellular response against viral infection that limits viral spread. Here, we show that a preexisting antiviral state inhibits the replication of influenza A viruses in human A549 cells by preventing transport of the viral genome to the nucleus and th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02220-13
更新日期:2013-12-01 00:00:00
abstract::While epidermal growth factor receptor (EGFR) has been shown to be important in the entry process for multiple viruses, including hepatitis C virus (HCV), the molecular mechanisms by which EGFR facilitates HCV entry are not well understood. Using the infectious cell culture HCV model (HCVcc), we demonstrate that the b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00750-12
更新日期:2012-10-01 00:00:00
abstract::In response to viral infection, host cells elicit a number of responses, including the expression of alpha/beta interferon (IFN-alpha/beta). In these cells, IFN regulatory factor-3 (IRF-3) undergoes a sequence of posttranslational modifications that allow it to act as a potent transcriptional coactivator of specific I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8411-8420.2004
更新日期:2004-08-01 00:00:00
abstract::Nevirapine forms the mainstay of our efforts to curtail the pediatric AIDS epidemic through prevention of mother-to-child transmission of HIV-1. A key limitation, however, is the rapid selection of HIV-1 strains resistant to nevirapine following the administration of a single dose. This rapid selection of resistance s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01010-10
更新日期:2010-10-01 00:00:00
abstract::Four virion activities of Newcastle disease virus (hemagglutinating, neuraminidase, hemolytic, and infectious activities) were examined before and after heat stress in low-salt buffer and physiological salt buffer (phosphate-buffered saline). The hemagglutinating and neuraminidase activities of the Australia-Victoria ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.18-26.1983
更新日期:1983-01-01 00:00:00
abstract::Previous experiments have demonstrated that adenovirus infection of human and mouse cells leads to an E1A-dependent activation of the DNA-binding capacity of a cellular transcription factor termed E2F. E2F binds to two sites in the adenovirus E2 early promoter which have been shown to be critical for E1A-dependent E2 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3643-3650.1989
更新日期:1989-09-01 00:00:00
abstract::Serological surveys have revealed that 30 to 50% of wild-caught African green monkeys have antibodies reactive to simian immunodeficiency virus (SIV), a retrovirus related to human immunodeficiency virus (HIV). Although the nucleotide sequence of one SIVagm isolate, Tyo1, was recently reported, the extent of genetic v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1800-1802.1989
更新日期:1989-04-01 00:00:00
abstract:UNLABELLED:The possibility that vaccination with adenovirus (AdV) vectors increased mucosal T cell activation remains a central hypothesis to explain the potential enhancement of HIV acquisition within the Step trial. Modeling this within rhesus macaques is complicated because human adenoviruses, including human adenov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03850-13
更新日期:2014-08-01 00:00:00
abstract::Herpesvirus lytic DNA replication requires both the cis-acting element, the origin, and trans-acting factors, including virally encoded origin-binding protein, DNA replication enzymes, and auxiliary factors. Two lytic DNA replication origins (ori-Lyt) of Kaposi's sarcoma-associated herpesvirus (KSHV) have been identif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01319-07
更新日期:2008-03-01 00:00:00
abstract::Interferon (IFN)-stimulated gene factor 2 (ISGF2) plays a role in transcription of the beta IFN (IFN-beta) gene and IFN-stimulated genes (ISGs) and may function as a central mediator of cytokine responses. Constitutive ISGF2 transgene expression resulted in substantial resistance to three RNA virus families. This phen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4470-4478.1992
更新日期:1992-07-01 00:00:00
abstract::Infectious bursal disease virus (IBDV) is a nonenveloped virus with an icosahedral capsid composed of two proteins, VP2 and VP3, that derive from the processing of the polyprotein NH(2)-pVP2-VP4-VP3-COOH. The virion contains VP1, the viral polymerase, which is both free and covalently linked to the two double-stranded...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3296-3303.2004
更新日期:2004-04-01 00:00:00
abstract::To clarify the role of core protein phosphorylation in pregenomic-RNA encapsidation of human and duck hepatitis B viruses (HBV and DHBV, respectively), we have examined the phosphorylation states of different forms of intracellular HBV core protein and the phenotypic effects of mutations in the phosphorylation sites o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4721-4728.2000
更新日期:2000-05-01 00:00:00
abstract::In vitro transcription of the avian tumor virus RNA by RNA-directed DNA polymerase is initiated on the unique cellular 4S RNA. Previous studies have shown that on the average there is one such RNA primer hydrogen bonded to each viral 35S RNA. The present study confirms that finding and demonstrates that, at least for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.3.553-558.1975
更新日期:1975-09-01 00:00:00
abstract::Partial deletion of the second hypervariable region from the envelope of the primary-like SF162 virus increases the exposure of certain neutralization epitopes and renders the virus, SF162DeltaV2, highly susceptible to neutralization by clade B and non-clade B human immunodeficiency virus (HIV-positive) sera (L. Stama...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5526-5540.2001
更新日期:2001-06-01 00:00:00
abstract::Adeno-associated viruses (AAVs) are small single-stranded DNA viruses that can package and deliver nongenomic DNA for therapeutic gene delivery. AAV8, a liver-tropic vector, has shown great promise for the treatment of hemophilia A and B. However, as with other AAV vectors, host anti-capsid immune responses are a dete...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00218-12
更新日期:2012-08-01 00:00:00
abstract::Using a method for freeze-drying intact cells, uninfected and murine leukemia virus (MuLV)-infected JLSV9 cell surfaces, as well as murine mammary tumor virus (MuMTV)-infected cell surfaces, were examined by electron microscopy. The 10-nm knobs of MuLV and the 5-nm spikes of MuMTV were clearly revealed on the surfaces...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.358-365.1977
更新日期:1977-01-01 00:00:00
abstract::Human papillomavirus type 1 (HPV1) E4 protein is associated with cytoplasmic and nuclear inclusions in productively infected keratinocytes. Here we have used transient expression of HPV1 E4 (also known as E1E4) protein in keratinocytes to reproduce formation of E4 inclusions. Immunofluorescence analysis showed that pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.673-684.2003
更新日期:2003-01-01 00:00:00
abstract::Our ability to rapidly respond to an emerging influenza pandemic is hampered somewhat by the lack of a susceptible small-animal model. To develop a more sensitive model, we pathotyped 18 low-pathogenic non-mouse-adapted influenza A viruses of human and avian origin in DBA/2 and C57BL/6 mice. The majority of the isolat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02444-09
更新日期:2010-08-01 00:00:00
abstract::Molluscum contagiosum virus (MCV), the only member of the Molluscipoxvirus genus, causes benign papules in healthy people but disfiguring lesions in immunocompromised patients. The sequence of MCV has been completed, revealing that MCV encodes a probable type I topoisomerase enzyme. All poxviruses sequenced to date al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3401-3406.1998
更新日期:1998-04-01 00:00:00
abstract::Like other herpesviruses, Kaposi's sarcoma-associated herpesvirus (KSHV, also designated human herpesvirus 8) can establish a latent infection in the infected host. During latency a small number of genes are expressed. One of those genes encodes latency-associated nuclear antigen (LANA), which is constitutively expres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6585-6594.2004
更新日期:2004-06-01 00:00:00
abstract::Mononuclear phagocytes (MP) and T lymphocytes play a pivotal role in the host immune response to human immunodeficiency virus type 1 (HIV-1) infection. Regulation of such immune responses can be mediated, in part, through the interaction of the T-lymphocyte-expressed molecule CD40 ligand (CD40L) with its receptor on M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4308-4320.2001
更新日期:2001-05-01 00:00:00
abstract::The phosphoprotein pp65 (ppUL83) of human cytomegalovirus (HCMV) is abundantly synthesized during lytic infection in cultured fibroblasts. As a major constituent of extracellular particles, it gains entry to infected cells immediately after adsorption and subsequently translocates to the cell nucleus. This efficient t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.5959-5968.1995
更新日期:1995-10-01 00:00:00
abstract::The hepatitis C virus (HCV) core protein is known to be a multifunctional protein, besides being a component of viral nucleocapsids. Previously, we have shown that the core protein binds to the cytoplasmic domain of lymphotoxin beta receptor, which is a member of tumor necrosis factor receptor (TNFR) family. In this s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3691-3697.1998
更新日期:1998-05-01 00:00:00
abstract::The RNAs of replication-defective murine and primate type C transforming viruses were analyzed for the presence of nucleotide sequences homologous to the genomes of their respective helper type C viruses by using DNAs complementary (cDNA) to either the 5'-terminal (cDNA5') or total (cDNAtotal) nucleotide sequences of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.570-576.1978
更新日期:1978-06-01 00:00:00
abstract::Luteoviruses and the luteovirus-like pea enation mosaic virus (PEMV; genus Enamovirus) are transmitted by aphids in a circulative, nonreplicative manner. Acquired virus particles persist for several weeks in the aphid hemolymph, in which a GroEL homolog, produced by the primary endosymbiont of the aphid, is abundantly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7258-7265.1997
更新日期:1997-10-01 00:00:00
abstract::Immunosuppressed patients are at risk for developing Epstein-Barr Virus (EBV)-positive lymphomas that express the major EBV oncoprotein, LMP1. Although increasing evidence suggests that a small number of lytically infected cells may promote EBV-positive lymphomas, the impact of enhanced lytic gene expression on the ab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00770-12
更新日期:2012-08-01 00:00:00
abstract::Strains of Theiler's murine encephalomyelitis virus (TMEV) are divided into two subgroups, TO and GDVII. TMEV strains show subgroup-specific virus growth and cell tropism and induce subgroup-specific diseases. Using site-directed mutagenesis, we demonstrated that the amino acid at position 57 of the leader protein (L(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00693-06
更新日期:2006-12-01 00:00:00