Abstract:
:A consensus binding site for the human papillomavirus (HPV) E2 protein was determined from an unbiased set of degenerate oligonucleotides using cyclic amplification and selection of targets (CASTing). Detectable DNA-protein complexes were formed after six to nine cycles of CASTing. A population of selected binding sites was cloned, and a consensus was determined by statistical analysis of the DNA sequences of individual isolates. Starting from a pool with 20 random bases, a consensus binding site of ACAC-N(5)-GGT was derived. CASTing and electrophoretic mobility shift analyses demonstrate that human but not bovine papillomavirus E2 proteins recognize this sequence. The presence of this sequence in papillomavirus genomes suggests a role for its function. We demonstrate that this site functionally substitutes for the canonical E2 binding site (ACCG-N(4)-CGGT) in both transient-transcription and DNA replication assays. This sequence, in most instances, is interchangeable with the resident E2 binding sites in the context of the HPV type 16 long control region. Where the novel sequence does not support E2-mediated effects on gene expression or DNA replication, we demonstrate that changing the orientation of the novel sequence restores this effect.
journal_name
J Viroljournal_title
Journal of virologyauthors
Newhouse CD,Silverstein SJdoi
10.1128/JVI.75.4.1722-1735.2001subject
Has Abstractpub_date
2001-02-01 00:00:00pages
1722-35issue
4eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
杂志文章abstract::The full-length viral RNA of human immunodeficiency virus type 1 (HIV-1) functions both as the mRNA for the viral structural proteins Gag and Gag/Pol and as the genomic RNA packaged within viral particles. The packaging signal which Gag recognizes to initiate genome encapsidation is in the 5' untranslated region (UTR)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02596-05
更新日期:2006-11-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.01073-13
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journal_title:Journal of virology
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doi:10.1128/JVI.64.2.936-940.1990
更新日期:1990-02-01 00:00:00
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doi:10.1128/JVI.02279-15
更新日期:2015-11-04 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.73.10.8571-8577.1999
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6688-6691.2004
更新日期:2004-06-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.45.1.73-79.1983
更新日期:1983-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6151-6156.1996
更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.73.2.1682-1688.1999
更新日期:1999-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00351-08
更新日期:2008-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
abstract::Fcgamma receptor (FcgammaR)-mediated entry of infectious dengue virus immune complexes into monocytes/macrophages is hypothesized to be a key event in the pathogenesis of complicated dengue fever. FcgammaRIA (CD64) and FcgammaRIIA (CD32), which predominate on the surface of such dengue virus-permissive cells, were com...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00792-06
更新日期:2006-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5526-5540.2001
更新日期:2001-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2922-2927.1993
更新日期:1993-05-01 00:00:00
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journal_title:Journal of virology
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更新日期:2008-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.6.2164-2172.1988
更新日期:1988-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00527-10
更新日期:2010-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.624-633.1985
更新日期:1985-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.1027-1038.1975
更新日期:1975-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1002-1009.1994
更新日期:1994-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00735-09
更新日期:2009-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.4734-4740.2002
更新日期:2002-05-01 00:00:00
abstract::By superinfection of human immunodeficiency virus type 2 (HIV-2) strain HIV-2ben-infected macaques with simian immunodeficiency virus (SIV) strain SIVmac, we investigated the mutual influences of an apathogenic and a pathogenic virus in vivo. Four rhesus and two cynomolgus monkeys were infected with HIV-2ben in 1988 a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1564-1574.1995
更新日期:1995-03-01 00:00:00