Abstract:
:Retinoic acid-inducible gene I (RIG-I) is a key sensor for recognizing nucleic acids derived from RNA viruses and triggers beta interferon (IFN-β) production. Because of its important role in antiviral innate immunity, the activity of RIG-I must be tightly controlled. Here, we used yeast two-hybrid screening to identify a SEC14 family member, SEC14L1, as a RIG-I-associated negative regulator. Transfected SEC14L1 interacted with RIG-I, and endogenous SEC14L1 associated with RIG-I in a viral infection-inducible manner. Overexpression of SEC14L1 inhibited transcriptional activity of the IFN-β promoter induced by RIG-I but not TANK-binding kinase 1 (TBK1) and interferon regulatory factor 3 (IRF3). Knockdown of endogenous SEC14L1 in both HEK293T cells and HT1080 cells potentiated RIG-I and Sendai virus-triggered IFN-β production as well as attenuated the replication of Newcastle disease virus. SEC14L1 interacted with the N-terminal domain of RIG-I (RIG-I caspase activation and recruitment domain [RIG-I-CARD]) and competed with VISA/MAVS/IPS-1/Cardif for RIG-I-CARD binding. Domain mapping further indicated that the PRELI-MSF1 and CRAL-TRIO domains but not the GOLD domain of SEC14L1 are required for interaction and inhibitory function. These findings suggest that SEC14L1 functions as a novel negative regulator of RIG-I-mediated antiviral signaling by preventing RIG-I interaction with the downstream effector.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li MT,Di W,Xu H,Yang YK,Chen HW,Zhang FX,Zhai ZH,Chen DYdoi
10.1128/JVI.01073-13subject
Has Abstractpub_date
2013-09-01 00:00:00pages
10037-46issue
18eissn
0022-538Xissn
1098-5514pii
JVI.01073-13journal_volume
87pub_type
杂志文章abstract:UNLABELLED:Hepadnaviruses (hepatitis B viruses [HBVs]) are the only animal viruses that replicate their DNA by reverse transcription of an RNA intermediate. Until recently, the known host range of hepadnaviruses was limited to mammals and birds. We obtained and analyzed the first amphibian HBV genome, as well as severa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00832-16
更新日期:2016-08-12 00:00:00
abstract::The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.6.1254-1262.1974
更新日期:1974-06-01 00:00:00
abstract::The 2009 pandemic influenza virus (pdm/09) has been frequently introduced to pigs and has reassorted with other swine viruses. Recently, H3N2 reassortants with pdm/09-like internal genes were isolated in Guangxi and Hong Kong, China. Genetic and epidemiological analyses suggest that these viruses have circulated in sw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06824-11
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abstract::Goat and rabbit antisera prepared against a purified Rauscher murine leukemia virus glycoprotein (gp69/71) rapidly neutralized spleen focus-forming virus in Rauscher and Friend virus preparations. Absorption studies revealed that most of the neutralizing activity of goat anti-Rauscher virus gp69/71 serum was directed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.187-189.1974
更新日期:1974-07-01 00:00:00
abstract::Open reading frame 45 (ORF45) of Kaposi's sarcoma-associated herpesvirus 8 (KSHV) is an immediate-early phosphorylated tegument protein and has been shown to play important roles at both early and late stages of viral infection. Homologues of ORF45 exist only in gammaherpesviruses, and their homology is limited. These...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02209-08
更新日期:2009-03-01 00:00:00
abstract::The herpes simplex virus 1 (HSV-1) multifunctional regulatory protein ICP27 shuttles between the nucleus and cytoplasm in its role as a viral mRNA export factor. Arginine methylation on glycine- and arginine-rich motifs has been shown to regulate protein export. ICP27 contains an RGG box and has been shown to be methy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00238-09
更新日期:2009-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05291-11
更新日期:2011-10-01 00:00:00
abstract::We have previously reported that infection of 9- to 13-day-old chicken embryos with RAV-1 results in rapid development of a novel B-cell lymphoma in which proviral insertion has activated expression of the c-myb gene (E. Pizer and E. H. Humphries, J. Virol. 63:1630-1640, 1989). The biological properties of these B-cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.1.512-523.1992
更新日期:1992-01-01 00:00:00
abstract::Glycans on human immunodeficiency virus (HIV) envelope protein play an important role in infection and evasion from host immune responses. To examine the role of specific glycans, we introduced single or multiple mutations into potential N-linked glycosylation sites in hypervariable regions (V1 to V3) of the env gene ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01691-07
更新日期:2008-01-01 00:00:00
abstract::The human T-cell leukemia virus type 1 Tax protein activates the expression of cellular immediate early genes controlled by the serum response element (SRE), which contains both the serum response factor (SRF) binding element (CArG box) and the ternary complex factor (TCF) binding element (Ets box). We show that TCF b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.23.11394-11397.2000
更新日期:2000-12-01 00:00:00
abstract::During latent herpes simplex virus type 1 (HSV-1) infection in the trigeminal ganglia of mice, three virus-specific transcripts, 2.0, 1.5, and 1.45 kilobases (kb), are detectable by Northern (RNA) blot analysis, but only the 2.0-kb transcript can be detected in HSV-1-infected tissue culture cells (J.G. Spivack and N. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3281-3287.1988
更新日期:1988-09-01 00:00:00
abstract::The Graffi murine leukemia virus (MuLV) is a retroviral mixture that induces predominantly myeloid leukemia in several inbred strains of mice. To analyze the viral component responsible for the myeloid leukemogenesis, we cloned several proviruses from a Graffi MuLV-infected cell line. Several infectious molecular clon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4722-4731.1993
更新日期:1993-08-01 00:00:00
abstract::Incorporation of human immunodeficiency virus type 1 (HIV-1) envelope proteins into vesicular stomatitis virus (VSV) particles was studied in a system that allows expressed envelope proteins to rescue phenotypically a temperature-sensitive mutant of VSV (tsO45). This mutant exhibits defective transport of its own enve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.360-365.1993
更新日期:1993-01-01 00:00:00
abstract::Apoptosis of neurons and astrocytes is induced by human immunodeficiency type 1 (HIV-1) infection in vitro and has been demonstrated in brain tissue from patients with AIDS. We analyzed a panel of diverse HIV-1 primary isolates for the ability to replicate and induce neuronal and astrocyte apoptosis in primary human b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.897-906.1999
更新日期:1999-02-01 00:00:00
abstract::The vaccinia virus F10 protein is one of two virally encoded protein kinases. A phenotypic analysis of infections involving a tetracycline-inducible recombinant (vDeltaiF10) indicated that F10 is involved in the early stages of virion morphogenesis, as previously reported for the mutants ts28 and ts15. The proteins en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2171-2190.2005
更新日期:2005-02-01 00:00:00
abstract::Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL) cells are predominantly infected with latent Kaposi's sarcoma-associated herpesvirus (KSHV), presenting a barrier to the destruction of tumor cells. Latent KSHV can be reactivated to undergo lytic replication. Here we report that in PEL cells, oxidative stress ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01742-10
更新日期:2011-01-01 00:00:00
abstract::Vertebrates mount a strong innate immune response against viruses, largely by activating the interferon system. Double-stranded RNA (dsRNA), a common intermediate formed during the life cycle of many viruses, is a potent trigger of this response. In contrast, no general inducible antiviral defense mechanism has been r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10442-10448.2004
更新日期:2004-10-01 00:00:00
abstract::The human immunodeficiency virus Tat protein is essential for virus replication and is a candidate vaccine antigen. Macaques immunized with Tat or chemically modified Tat toxoid having the same clade B sequence developed strong antibody responses. We compared these antisera for their abilities to recognize diverse Tat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3157-3166.2003
更新日期:2003-03-01 00:00:00
abstract::Therapeutic suppression of human immunodeficiency virus type 1 (HIV-1) replication may help elucidate interactions between the host cellular immune responses and HIV-1 infection. We performed a detailed longitudinal evaluation of two subjects before and after the start of highly active antiretroviral therapy (HAART). ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6721-6728.1999
更新日期:1999-08-01 00:00:00
abstract::Epstein-Barr virus (EBV)-induced B-cell growth transformation, a central feature of the virus' strategy for colonizing the human B-cell system, requires full virus latent gene expression and is initiated by transcription from the viral promoter Wp. Interestingly, when EBV accesses other cell types, this growth-transfo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10458-10467.2000
更新日期:2000-11-01 00:00:00
abstract::Intracellular simian virus 40 (SV40) chromatin was photoreacted with a 3H-labeled psoralen derivative, hydroxymethyltrimethylpsoralen (HMT), at 48 h postinfection. Psoralen compounds have been shown to readily penetrate intact cells and, in the presence of long-wavelength UV light, form covalent adducts to DNA, prefer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.78-87.1982
更新日期:1982-01-01 00:00:00
abstract::Nef is a regulatory gene product of human immunodeficiency virus type 1 (HIV-1) and other primate lentiviruses which enhances virion infectivity by an unknown mechanism. We report here that Nef is detectable at moderate levels in preparations of HIV-1 virions which lack active viral protease (PR). Significantly smalle...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1013-1018.1997
更新日期:1997-02-01 00:00:00
abstract::The goal of an effective AIDS vaccine is to generate immunity that will prevent human immunodeficiency virus 1 (HIV-1) acquisition. Despite limited progress toward this goal, renewed optimism has followed the recent success of the RV144 vaccine trial in Thailand. However, the lack of complete protection in this trial ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00315-12
更新日期:2012-05-01 00:00:00
abstract::Oncostatin M (OSM) is released together with type I interferon (IFN) by activated dendritic cells, suggesting a concerted action of these cytokines in the biological response against infection. We found that OSM increases the antiviral effect of IFN-alpha in Huh7 hepatoma cells infected with hepatitis A or hepatitis C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02167-08
更新日期:2009-04-01 00:00:00
abstract::Two chimpanzees immunized with woodchuck hepatitis virus (WHV) surface antigen (WHsAg) developed antibodies cross-reactive with hepatitis B virus (HBV) surface antigen (HBsAg). After challenge with HBV, one animal was completely protected and the other experienced a subclinical infection, without evidence of liver dis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.895-901.1986
更新日期:1986-12-01 00:00:00
abstract::Activation of the interferon-inducible, double-stranded RNA-dependent protein kinase was monitored in monolayer cultures of control and interferon-treated HeLa cells infected with encephalomyocarditis virus. The extent of phosphorylation in the intact cell of the alpha-subunit of eucaryotic protein synthesis initiatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.3.894-898.1985
更新日期:1985-06-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infects human cells by binding to surface CD4 molecules and directly fusing with the cell membrane. Although mouse cells expressing human CD4 bind HIV, they do not become infected, apparently because of a block in membrane fusion. To study this problem, we constructed a recombinant v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2149-2156.1990
更新日期:1990-05-01 00:00:00
abstract::Corynebacteriophages beta and gamma are closely related but heteroimmune; hence, gamma reproduces in C7(beta). A series of gamma mutants, designated gamma-bin (beta-inhibited), has been isolated. They reproduce in only 2 to 14% of infected C7(beta) cells, and, as a result, plaque with an efficiency of 10(-4) to 10(-5)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.28-33.1978
更新日期:1978-10-01 00:00:00
abstract::Treatment of patients with lamivudine (3TC) results in loss of detectable levels of hepatitis B virus (HBV) DNA from serum; however, the relapse rate, with regard to both reappearance of virus in the bloodstream and hepatic inflammation, is high when therapy is terminated. Although the rebound observed in patients has...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8148-8160.2002
更新日期:2002-08-01 00:00:00
abstract::Hemagglutinin (HA) of influenza virus must be activated by proteolysis before the virus can become infectious. Previous studies indicated that HA cleavage is driven by membrane-bound or extracellular serine proteases in the respiratory tract. However, there is still uncertainty as to which proteases are critical for a...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2017-07-27 00:00:00